Abstract:
:By using a combination of biochemical methods (i.e., endoglycosidase H digestion and immunoblot and plant lectin binding studies), it was verified that pig flavin-containing monooxygenase (FMO1) was N-glycosylated. By using mass spectrometry approaches [i.e., peptide mapping, gas chromatography/mass spectrometry, microbore HPLC/electrospray ionization mass spectrometry (LC/ESI/MS), chemical ionization gas chromatography/mass spectrometry (CI/GC/MS), and matrix-assisted laser desorption mass spectrometry (MALDI/MS)], we were able to confirm that pig FMO1 was N-glycosylated and we were able to identify the site of N-glycosylation. Pig FMO1 contains two putative consensus sites of N-glycosylation. The results showed that pig FMO1 amino acid Asn120 was selectively N-glycosylated. Highly purified pig FMO1 avidly bound concanavalin A and reacted positively for carbohydrates by the periodic acid/Schiff's base method of analysis. In addition, treatment of pig FMO1 with endo-N-acetylglucosaminidase converted the enzyme to another species with a molecular mass approximately 5000 Da lower than that of the parent protein as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot experiments. Peptide mapping of pig FMO1 showed that the protein used in the study was not contaminated with another glycoprotein. MALDI/MS experiments showed that pig FMO1 was present with the expected molecular mass but that higher-molecular mass forms consistent with the presence of N-linked high-mannose oligosaccharide structures were also covalently attached to the enzyme. The presence of N-acetylglucosamine isolated from acid hydrolysates of the N-linked high-mannose oligosaccharide of pig FMO1 was confirmed by high-pH anion exchange HPLC studies and verified by CI/GC/MS studies of derivatized monosaccharide fractions. Further analysis of pig FMO1 proteolytic peptides by LC/ESI/MS showed that the only residue that was N-glycosylated in pig FMO1 was Asn120. Knowledge of the structural aspects of FMO may be useful in understanding the membrane association properties of the enzyme.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Korsmeyer KK,Guan S,Yang ZC,Falick AM,Ziegler DM,Cashman JRdoi
10.1021/tx980117psubject
Has Abstractpub_date
1998-10-01 00:00:00pages
1145-53issue
10eissn
0893-228Xissn
1520-5010pii
tx980117pjournal_volume
11pub_type
杂志文章abstract::The relation between organophosphorus-induced delayed neuropathy (OPIDN) and brain neuropathy target esterase (NTE) inhibition is further examined in hens by structure-activity studies leading to the most potent in vitro NTE inhibitors known, which are then examined for their neuropathic effects in vivo in hens. The p...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00050a011
更新日期:1995-12-01 00:00:00
abstract::The seaweed Fucus vesiculosus is unusual when compared with other algal species, in that it can survive in toxic-metal-contaminated aquatic environments. The metallothionein gene has been identified in F. vesiculosus by Kille and co-workers (Morris, C. A., Nicolaus, B., Sampson, V., Harwood, J. L., and Kille, P. (1999...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx050206j
更新日期:2006-03-01 00:00:00
abstract::Cryogenic (4-10 K) laser-induced vibrationless ground state and vibronic excited state fluorescence emission spectra of the adducts resulting from reaction in vitro of human serum albumin and the carcinogen (+-)-r-7,t-8-dihydroxy-c-9,c-10-epoxy-7,8,9,10- tetrahydrobenzo[a]-pyrene were recorded in order to determine th...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00025a012
更新日期:1992-01-01 00:00:00
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journal_title:Chemical research in toxicology
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更新日期:2002-05-01 00:00:00
abstract::Taconite mining involves potential exposure to non-asbestiform amphibole mineral fiber. More recent studies have demonstrated increased mortality from respiratory cancers and heart disease among workers in the taconite industry. This finding is not consistent with recent exposure assessment findings, nor is the toxico...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.5b00472
更新日期:2016-02-15 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.5b00130
更新日期:2015-07-20 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2020-08-17 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章,评审
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更新日期:2012-01-13 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00026a022
更新日期:1992-03-01 00:00:00
abstract::2'-Deoxyoxanosine (dOxo) is a novel DNA lesion produced from dGuo by reaction with nitrous acid or nitric oxide [Suzuki, T., Yamaoka, R., Nishi, M., Ide, H., and Makino, K. (1996) J. Am. Chem. Soc. 118, 2515-2516]. We investigated the reaction of dOxo with glycine (Gly) under physiological conditions. When 5 mM dOxo w...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx990164x
更新日期:2000-04-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx034194i
更新日期:2004-03-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx00043a021
更新日期:1995-01-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2014-05-19 00:00:00
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journal_title:Chemical research in toxicology
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doi:10.1021/tx0000926
更新日期:2000-09-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx300017s
更新日期:2012-07-16 00:00:00
abstract::The nuclear receptor human pregnane X receptor (hPXR) is a ligand-regulated transcription factor that responds to a wide range of endogenous and xenobiotic molecules. Upon activation with ligands, hPXR can increase induction levels of metabolic enzymes. Therefore, hPXR plays a critical role in drug metabolism and excr...
journal_title:Chemical research in toxicology
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更新日期:2011-10-17 00:00:00
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journal_title:Chemical research in toxicology
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journal_title:Chemical research in toxicology
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
abstract::Interstrand cross-links are minor components of the collection of products formed in DNA by ionizing radiation. Through their formation by other damaging agents, it is known that interstrand cross-links exert significant effects on replication and transcription. The structures of DNA interstrand cross-links produced a...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx7002307
更新日期:2007-11-01 00:00:00
abstract::Reactive oxygen species can induce the formation of tandem DNA lesions. We recently showed that the treatment of calf thymus DNA with Cu2+/H2O2/ascorbate could result in the efficient formation of a tandem lesion where a 5,6-dihydroxy-5,6-dihydrothymidine (or thymidine glycol) is situated on the 5' side of an 8-oxo-7,...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2009-03-16 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:2013-11-18 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章,评审
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更新日期:2011-11-21 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.5b00166
更新日期:2015-07-20 00:00:00
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journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/acs.chemrestox.5b00032
更新日期:2015-04-20 00:00:00
abstract::We have previously reported that mono- and dichlorinated biphenyls (PCBs) can be metabolized to dihydroxy compounds and further oxidized to reactive metabolites which form adducts with nitrogen and sulfur nucleophiles including DNA [Amaro et al. (1966) Chem. Res. Toxicol. 9, 623-629; Oakley et al. (1996) Carcinogenesi...
journal_title:Chemical research in toxicology
pub_type: 杂志文章
doi:10.1021/tx960103o
更新日期:1996-12-01 00:00:00