Abstract:
:Purine deoxyribonucleoside 3'-phosphates were reacted separately with the four configurational isomers of benzo[c]phenanthrene 3,4-dihydrodiol 1,2-epoxide. Products resulting from the cis and trans opening of the epoxide ring by the exocyclic amino groups of deoxyadenosine and deoxyguanosine 3'-phosphates were separated by high-pressure liquid chromatography and identified by comparison of the observed circular dichroism spectra with the known spectra for the corresponding nucleoside adducts. The 16 structurally identified benzo[c]phenanthrene-purine deoxyribonucleoside 3'-phosphate adducts were then separately postlabeled according to the Randerath method, and the positions of the individual bisphosphates were mapped by thin-layer chromatography. Chromatographic conditions were developed that allowed separation of the four adducts for 3 of the 4 dihydrodiol epoxide isomers.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Canella KA,Peltonen K,Yagi H,Jerina DM,Dipple Adoi
10.1021/tx00029a015subject
Has Abstractpub_date
1992-09-01 00:00:00pages
685-90issue
5eissn
0893-228Xissn
1520-5010journal_volume
5pub_type
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