Abstract:
:The extended (E) isoform unique to Fibrinogen420 (Fib420) is distinguished from the conventional chain of Fibrinogen340 by the presence of an additional 236-residue carboxyl terminus globular domain (EC). A recombinant form of EC (rEC), having a predicted mass of 27,653 Daltons, was expressed in yeast (Pichia pastoris) and purified by anion exchange column chromatography. Purified rEC appears to be predominantly intact, as judged by N-terminal sequence analysis, mass spectral analysis of the C-terminal cyanogen bromide (CNBr) fragment, and comparison of recognition by epitope-specific monoclonal antibodies. Carbohydrate determination, coupled with analysis of CNBr digestion fragments, confirms N-linked glycosylation at Asn667, the site at which sugar is attached in E. Analysis of CNBr digestion fragments confirms that two disulfide bridges exist at cysteine pairs E613/644 and E780/793. In the presence of 5 mmol/L EDTA, rEC is highly susceptible to plasmic degradation, but Ca2+ (5 mmol/L) renders rEC resistant. No protective effect from plasmic degradation was conferred to rEC by the peptides GPRPamide or GHRP, nor did rEC bind to a GPR peptide column. These results suggest that the EC domain contains a calcium-binding site, but lacks a polymerization pocket. By analogy with the site elucidated in the gammaC domain, we predict that the EC calcium binding site involves residues E772-778: DADQWEE.
journal_name
Bloodjournal_title
Bloodauthors
Applegate D,Haraga L,Hertzberg KM,Steben LS,Zhang JZ,Redman CM,Grieninger Gsubject
Has Abstractpub_date
1998-11-15 00:00:00pages
3669-74issue
10eissn
0006-4971issn
1528-0020journal_volume
92pub_type
杂志文章相关文献
BLOOD文献大全abstract::Interferon (IFN)-alpha inhibits DNA synthesis stimulated by low molecular weight B-cell growth factor (BCGF) in hairy cells in vitro, suggesting that the therapeutic efficacy of IFN-alpha in hairy cell leukemia (HCL) involves growth inhibition of malignant B cells. Evidence that the 16-Kd cell surface protein Leu-13 m...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-12-15 00:00:00
abstract::Cerebral malaria is a severe multifactorial condition associated with the interaction of high numbers of infected erythrocytes to human brain endothelium without invasion into the brain. The result is coma and seizures with death in more than 20% of cases. Because the brain endothelium is at the interface of these pro...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-06-226415
更新日期:2009-11-05 00:00:00
abstract::Antibody-induced antigenic modulation (AIAM) of CD10 and CD19 was studied on NALM-6, RAJI, and JOK-1 cell lines using fluorescence microscopy (FM), flow cytometry (FCM), and immunoelectron microscopy (IEM). Cross-linking with monoclonal antibodies (MoAbs) induced rapid redistribution of CD10 and CD19 on the cell surfa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-03-15 00:00:00
abstract::Levels of erythrocyte glycolytic intermediates after the phosphofructokinase (PFK) step, including 2,3-bisphosphoglycerate (2,3-DPG), were decreased at rest in patients from separate families with type VII glycogenosis. The concentration of 2,3-DPG was about half of the normal control value during a period of unrestri...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
abstract::Before the contemporary development of rationally designed antineoplastic therapies, cladribine was identified as a lymphocyte-specific agent. Its profound impact on the natural history of hairy cell leukemia, with responses approaching 100% and a median duration of response of nearly a decade after only a single 7-da...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-02-246140
更新日期:2010-10-21 00:00:00
abstract::Our previous study demonstrated that 2-methoxyestradiol (2ME2), an estrogen derivative, induces apoptosis in multiple myeloma (MM) cells; however, the related transcriptional events are unclear. In the present study, we used oligonucleotide microarrays to identify genes altered during 2ME2-induced apoptosis in MM cell...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3146
更新日期:2003-05-01 00:00:00
abstract::Most juvenile chronic myelogenous leukemia (JCML) cells have limited long-term proliferative capacity, and only a minority of immature cells give rise to colonies in semisolid cultures. Clonogenic JCML progenitors cannot be maintained in culture because they differentiate, and within a few weeks the leukemic clone is ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::Reduced oxygenation of a variety of cells results in transcriptional upregulation of several genes, including the hematopoietic hormone erythropoietin, the angiogenic vascular endothelial growth factor (VEGF), and glycolytic enzymes such as aldolase. Recently, the heme protein cytochrome b558 of the nicotinamide adeni...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-01-15 00:00:00
abstract::SCL/tal is a putative oncogene originally identified through its involvement in the translocation t(1;14)(p32;q11) present in the leukemic cell line DU.528. Subsequent studies have shown an upstream deletion activating expression of SCL/tal to be one of the most common genetic lesions in T-cell acute lymphoblastic leu...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Epithin/PRSS14, a type II transmembrane serine protease, is involved in normal epithelial development and tumor progression. Here we report, as an interacting substrate of epithin, a receptor tyrosine kinase Tie2 that is well known for important roles in the vessel stability. Epithin interacts with and degrades the Ti...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-03-275289
更新日期:2011-01-27 00:00:00
abstract::One hundred forty-two patients between the ages of 1 and 17 years who survived disease-free more than 1 year after marrow transplantation for hematologic malignancy had growth and development evaluations from one to 14 years posttransplant (median 4 years). Prior to transplant all children received multiagent chemothe...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
abstract::The PIT1/SLC20A1 protein, a well-described sodium/phosphate cotransporter and retrovirus receptor, has been identified recently as a modular of proliferation and apoptosis in vitro. The targeted deletion of the PIT1 gene in mice revealed a lethal phenotype due to severe anemia attributed to defects in liver developmen...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-05-427302
更新日期:2013-01-24 00:00:00
abstract::The transcription factor signal transducer and activator of transcription 5 (STAT5) fulfills essential roles in self-renewal in mouse and human hematopoietic stem cells (HSCs), and its persistent activation contributes to leukemic transformation, although little molecular insight into the underlying mechanisms has bee...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-08-303669
更新日期:2011-03-24 00:00:00
abstract::Two kinds of erythrocytes are released in the blood of irradiated adult hybrid mice grafted with parental fetal liver cells: fetal antigen-bearing erythrocytes (Ft+ cells) and adult-type Ft- erythrocytes. Both are of parental origin, as determined by immune lysis using histocompatibility alloantigens. The latter cells...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::Interleukin (IL)-23 is a proinflammatory cytokine belonging to the IL-12 superfamily. The antitumor activity of IL-23 is controversial, and it is unknown whether or not the cytokine can act directly on tumor cells. The aim of this study was to investigate the potential direct antitumor activity of IL-23 in pediatric B...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-10-248245
更新日期:2010-11-11 00:00:00
abstract::Natural killer (NK) cell activity is severely impaired in untreated patients with hairy cell leukemia (HCL). In an attempt to investigate whether this impairment is related to a defect at the target cell binding and/or at the post target cell binding level, we evaluated the peripheral blood mononuclear cells (PBMC) of...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Current monoclonal antibody therapies for multiple myeloma have had limited success, perhaps due to narrow target specificity. We have previously described the ability of polyclonal rabbit antithymocyte globulin (rATG) to induce caspase- and cathepsin-mediated apoptosis in human B and plasma cells. We now extend this ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-06-2269
更新日期:2006-04-01 00:00:00
abstract::An understanding of the number and contribution of individual pluripotent hematopoietic stem cells (HSCs) to the formation of blood lineages has important clinical implications for gene therapy and stem cell transplantation. We have been able to efficiently mark rhesus macaque long-term repopulating stem and progenito...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-08-2935
更新日期:2004-09-01 00:00:00
abstract::Human early thymic precursors have the potential to differentiate into multiple cell lineages, including T cells and plasmacytoid dendritic cells (pDCs). This decision is guided by the induction or silencing of lineage-specific transcription factors. The ETS family member Spi-B is a key regulator of pDC development, w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-05-2090
更新日期:2006-03-15 00:00:00
abstract::Recent studies suggest that the evolutionary history of a cancer is important in forecasting clinical outlook. To gain insight into the clonal dynamics of multiple myeloma (MM) and its possible influence on patient outcomes, we analyzed whole exome sequencing tumor data for 333 patients from Myeloma XI, a UK phase 3 t...
journal_title:Blood
pub_type: 杂志文章,随机对照试验
doi:10.1182/blood-2016-11-750612
更新日期:2017-10-05 00:00:00
abstract::In this issue of Blood, Finn et al have taken a factor IX variant with increased specific activity associated with thrombophilia and used it to improve gene therapy of hemophilia B in dogs, and Cantore et al have shown similar results in mice. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-09-452821
更新日期:2012-11-29 00:00:00
abstract::The mammalian target of rapamycin (mTOR) plays crucial roles in proliferative and antiapoptotic signaling in lymphoid malignancies. Rapamycin analogs, which are allosteric mTOR complex 1 (mTORC1) inhibitors, are active in mantle cell lymphoma and other lymphoid neoplasms, but responses are usually partial and short-li...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-346601
更新日期:2012-01-12 00:00:00
abstract::Increasing evidence indicates that the gut microbiota is closely associated with acute graft-versus-host disease (aGVHD) in stem cell transplantation (SCT). Fecal microbiota transplantation (FMT) could represent an alternative treatment option for aGVHD. However, FMT for SCT patients carries a potential risk of infect...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2016-05-717652
更新日期:2016-10-20 00:00:00
abstract::The transcription factor CCAAT enhancer binding protein α (C/EBPα) is a master regulator in granulopoiesis and is frequently disrupted in acute myeloid leukemia (AML). We have previously shown that C/EBPα exerts its effects by regulating microRNAs (miRs) such as miR-223 and miR-34a. Here, we confirm miR-30c as a novel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-12-472183
更新日期:2013-10-03 00:00:00
abstract::The anemia of chronic disease (ACD) results from 3 major processes: slightly shortened red cell survival, impaired reticuloendothelial system iron mobilization, and impaired erythropoiesis. Hepcidin is an acute-phase protein with specific iron regulatory properties, which, along with the anemia seen with increased hep...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-07-2854
更新日期:2006-04-01 00:00:00
abstract::We assessed the effects of the c-kit ligand, stem cell factor (SCF), in the jejunal mucosal mast cell hyperplasia that occurs during infection with the intestinal nematodes, Nippostrongylus brasiliensis or Trichinella spiralis in rats. Compared with vehicle-treated rats, rats treated with SCF (25 micrograms/kg/d, intr...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Surface receptors specific for either the C4b (CR1) or C3d (CR2) component of complement were examined on the neoplastic cells from 30 cases of non-Hodgkin's lymphoma of B-cell origin and on cells derived from 9 normal lymphoid tissues. Lymphocyte suspensions from non-neoplastic peripheral blood, tonsils, and lymph no...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::The outcome of patients with acute myeloid leukemia who are older than 60 years has remained poor because of unfavorable disease characteristics and patient-related factors. The randomized German-Austrian AML Study Group 06-04 protocol was designed on the basis of in vitro synergistic effects of valproic acid (VPA) an...
journal_title:Blood
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2013-12-546283
更新日期:2014-06-26 00:00:00
abstract::ras gene mutations are the most frequent molecular changes found in the preleukemic syndromes of adults and may play a role in initiating these diseases and in their progression to acute leukemia. However, little is known about the incidence or importance of these genetic mutations in childhood myeloproliferative stat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::Thymosin beta 4 (T beta 4) was originally isolated as a thymic hormone. Its functional properties remain obscure; however, the N-terminal peptidic sequence could have a regulatory function on hematopoietic stem cell proliferation. To investigate the mechanism of T beta 4 expression, we studied T beta 4 gene expression...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00