Abstract:
:We have isolated a human cDNA clone encoding a novel protein of 22 kDa that is a human counterpart of the rat oncoprotein PTTG. We show that the corresponding gene (hpttg) is overexpressed in Jurkat cells (a human T lymphoma cell line) and in samples from patients with different kinds of hematopoietic malignancies. Analysis of the sequence showed that hPTTG has an amino-terminal basic domain and a carboxyl-terminal acidic domain, and that it is a proline-rich protein with several putative SH3-binding sites. Subcellular fractionation studies show that, although hPTTG is mainly a cytosolic protein, it is partially localized in the nucleus. In addition we demonstrate that the acidic carboxyl-terminal region of hPTTG acts as a transactivation domain when fused to a heterologous DNA binding domain, both in yeast and in mammalian cells.
journal_name
Oncogenejournal_title
Oncogeneauthors
Domínguez A,Ramos-Morales F,Romero F,Rios RM,Dreyfus F,Tortolero M,Pintor-Toro JAdoi
10.1038/sj.onc.1202140subject
Has Abstractpub_date
1998-10-29 00:00:00pages
2187-93issue
17eissn
0950-9232issn
1476-5594journal_volume
17pub_type
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