Abstract:
:The zinc-finger transcription factor Krox24 was analysed for its role in differentiation in P19 embryonal carcinoma cells. Reciprocal dominant negative mutants consisting of Krox24 deleted for a crucial region of the zinc-finger domain (delta Krox24) or of the zinc-finger region alone (delta Krox24Zf) abolished the activation of transcription by Krox24 in P19 cells. Expression of Krox24 led to spontaneous differentiation of P19 cells in a lineage-independent fashion. Krox24 transfected populations, as well as individual clones randomly picked from them, displayed a wide array of diverse morphologies and expressed markers characteristic of a variety of differentiated cells. The dominant negative mutants blocked differentiation of P19 cells. We conclude that expression of Krox24 is sufficient for pluripotent differentiation of embryonal carcinoma cells, and that expression of Krox24 or other egr family members is essential to this process.
journal_name
Oncogenejournal_title
Oncogeneauthors
Lanoix J,Mullick A,He Y,Bravo R,Skup Ddoi
10.1038/sj.onc.1202166subject
Has Abstractpub_date
1998-11-12 00:00:00pages
2495-504issue
19eissn
0950-9232issn
1476-5594journal_volume
17pub_type
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