Separation of phospholipase A2 in Habu snake venom by glycyrrhizin (GL)-affinity column chromatography and identification of a GL-sensitive enzyme.

abstract：:Ogi-Keishi-Gomotsu-To-Ka-Kojin (OKGK) is a traditional herbal medicine (Kampo medicine) which has been found to ameliorate hypercholesterolemia and hypertriglyceridemia in rats and rabbits. In the present study, the effect of OKGK on acyl-CoA:cholesterol acyltransferase (ACAT) was studied in order to elucidate the mec...

journal_title：Biological & pharmaceutical bulletin

authors： Wu CZ,Inoue M,Ogihara Y

更新日期：1999-09-01 00:00:00

abstract：:Costunolide, isolated from the stem bark of Magnolia sieboldii, is a sesquiterpene lactone that exhibits various biological and immunological actions. We investigated the induction mechanism of apoptosis by costunolide in a human B cell leukemia NALM-6 cell culture system. Costunolide (10 microM)-induced apoptosis tim...

journal_title：Biological & pharmaceutical bulletin

authors： Kanno S,Kitajima Y,Kakuta M,Osanai Y,Kurauchi K,Ujibe M,Ishikawa M

更新日期：2008-05-01 00:00:00

abstract：:An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN pa...

journal_title：Biological & pharmaceutical bulletin

authors： Yamada Y,Kogure K,Nakamura Y,Inoue K,Akita H,Nagatsugi F,Sasaki S,Suhara T,Harashima H

更新日期：2005-10-01 00:00:00

abstract：:Over-expression of P-glycoprotein (P-gp) in lymphocytes is implicated in the failure of immunosuppressant therapy. We investigated P-gp function in peripheral-blood CD3+, CD4+, and CD8+ cells of 14 healthy subjects and 12 patients with systemic lupus erythematosus (SLE). P-gp function was estimated by the transporter ...

journal_title：Biological & pharmaceutical bulletin

authors： Henmi K,Yoshida M,Yoshikawa N,Hirano T

更新日期：2008-05-01 00:00:00

abstract：:Meiosis activating sterol (MAS), the intermediate of cholesterol biosynthesis, is an important substance to stimulate oocytes maturation in FSH-induced signal transduction pathway. Lanosterol 14alpha-demethylase (CYP51) converts lanosterol to MAS. Although MAS is firstly isolated from bovine testis, the information ab...

journal_title：Biological & pharmaceutical bulletin

authors： Wang F,Shen Y,Song X,Xia G,Chen X,Zhou B,Lei L

更新日期：2006-03-01 00:00:00

abstract：:Resveratrol, which is found in grapes, red wine, and berries, has many beneficial health effects, such as anti-cancer, neuro-protective, anti-inflammatory, and life-prolonging effects. However, the cellular mechanisms by which resveratrol acts are relatively unknown, especially in terms of possible regulation of recep...

journal_title：Biological & pharmaceutical bulletin

authors： Lee BH,Hwang SH,Choi SH,Shin TJ,Kang J,Lee SM,Nah SY

更新日期：2011-01-01 00:00:00

abstract：:Significant selective cyclooxygenase-2 (COX-2) inhibitory activities were observed for two new 1,4-naphthoquinone sodium salts, sodium 3-hydroxide-2[[sodium 3-hydroxide-1,4-dioxo(2-naphthyl)]ethyl]naphthalene-1,4-dione (impatienolate) (1) and sodium 2-hydroxide-3-(2-hydroxyethyl)naphthalene-1,4-dione (balsaminolate) (...

journal_title：Biological & pharmaceutical bulletin

authors： Oku H,Ishiguro K

更新日期：2002-05-01 00:00:00

abstract：:This study sought to investigate the ameliorating effects of soy 11S protein on the impacts of alcohol consumption in rat hepatocytes and in reducing total cholesterol levels and total lipid levels in the serum. Liver histology and the clinically important enzyme markers (Aspartate Aminotransferase: AST and Alanine Am...

journal_title：Biological & pharmaceutical bulletin

authors： Park KJ,Kim HY,Chang BJ,Lee HH

更新日期：2004-10-01 00:00:00

abstract：:Isofatty acid-containing phosphatidylglycerol (Fr. 7-C), isolated from Bacillus stearothermophilus UBT8038, enhances the induction of concanavalin A (Con A)-activated suppressor T (Ts) cells in a dose dependent manner (0.01-1 microgram/ml). Its further biological properties on mouse mixed lymphocyte reaction (MLR) has...

journal_title：Biological & pharmaceutical bulletin

authors： Kohama Y,Semba T,Tanaka K,Iida K,Moriyama K,Suda T,Tone H,Itoh S,Mimura T

更新日期：1994-11-01 00:00:00

abstract：:All living organisms on earth are composed of L-amino acids and D-sugars. Therefore the presence and function of D-amino acids in living organisms have not been studied. Recently, however, D-aspartic acid (D-Asp) which is a D-amino acid, has been detected in various proteins from human tissue samples such as eye lens,...

journal_title：Biological & pharmaceutical bulletin

authors： Fujii N

更新日期：2005-09-01 00:00:00

abstract：:In order to study the disposition of benzphetamine (BZP) and its metabolites, desmethyl benzphetamine (norBZP), p-hydroxy desmethyl benzphetamine (OHnorBZP), methamphetamine (MA) and amphetamine (AP), from plasma to hair in rats, an analytical method for identifying these drugs in plasma, urine and hair was developed ...

journal_title：Biological & pharmaceutical bulletin

authors： Kikura R,Nakahara Y

更新日期：1995-12-01 00:00:00

abstract：:A plasmid-cured strain of the marine Vibrio alginolyticus 138-2 retains a respiration-driven Na+ pump. Examinations of several strains of V. alginolyticus and V. parahaemolyticus revealed that these murine Vibrio always possessed the respiration-driven Na+ pump irrespective of the presence or absence of plasmids. Thes...

journal_title：Biological & pharmaceutical bulletin

authors： Nakamura T,Hayashi S,Yamanaka R,Hamashima H,Arai T,Unemoto T

更新日期：1993-08-01 00:00:00

abstract：:The hydrodynamic method by rapid intravenous injection of a large volume of plasmid DNA is known to be an efficient and liver-specific method of in vivo gene delivery and achieves high levels of foreign gene expression, particularly in hepatocytes. Low transgene activities have also been observed in other organs such ...

journal_title：Biological & pharmaceutical bulletin

authors： Hattori Y,Koga K,Izumisawa T,Yamasaki M,Narishima R,Yoshida S,Fukui T,Maitani Y

更新日期：2009-04-01 00:00:00

abstract：:Recently, a silent polymorphism of C3435T of the MDR1 gene, encoding the multidrug resistant transporter MDR1/P-glycoprotein, has been found to be associated with susceptibility to ulcerative colitis (UC), but this remains controversial. This study was conducted to find a possible reason for the discrepancies, and it ...

journal_title：Biological & pharmaceutical bulletin

authors： Osuga T,Sakaeda T,Nakamura T,Yamada T,Koyama T,Tamura T,Aoyama N,Okamura N,Kasuga M,Okumura K

更新日期：2006-02-01 00:00:00

abstract：:Catalase protects cells from reactive oxygen species-induced damage by catalyzing the breakdown of hydrogen peroxide to oxygen and water. Arsenite decreases catalase activity; it activates phosphatidylinositol 3-kinase (PI3K) and its key downstream effector Akt in a variety of cells. The PI3K pathway is known to inhib...

journal_title：Biological & pharmaceutical bulletin

authors： Kim S,Lee SH,Kang S,Lee L,Park JD,Ryu DY

更新日期：2011-01-01 00:00:00

abstract：:We studied the protective effects of Echinacea purpurea against radiation by evaluating changes in the peripheral blood cell count and peripheral blood antioxidant activity. E. purpurea administration had a suppressive effect on radiation-induced leukopenia, especially on lymphocytes and monocytes, and resulted in a f...

journal_title：Biological & pharmaceutical bulletin

authors： Mishima S,Saito K,Maruyama H,Inoue M,Yamashita T,Ishida T,Gu Y

更新日期：2004-07-01 00:00:00

abstract：:Despite the relatively high prevalence of migraine or headache, the pathophysiological mechanisms triggering headache-associated peripheral hypersensitivities, are unknown. Since nitric oxide (NO) is well known as a causative factor in the pathogenesis of migraine or migraine-associated hypersensitivities, a mouse mod...

journal_title：Biological & pharmaceutical bulletin

authors： Kim SJ,Yeo JH,Yoon SY,Kwon SG,Lee JH,Beitz AJ,Roh DH

更新日期：2018-02-01 00:00:00

abstract：:Nafamostat mesilate, an ester drug with extensive hydrolysis in vivo, exhibits species difference in the relative contribution for its hydrolysis in blood and tissues. For the rat, the main hydrolysis site may be blood and human may be tissue (mainly by liver). The paper gave in vivo evidence that human tissue may giv...

journal_title：Biological & pharmaceutical bulletin

authors： Cao YG,Chen YC,Hao K,Zhang M,Liu XQ

更新日期：2008-11-01 00:00:00

abstract：:The medial prefrontal cortex (mPFC) plays critical roles in the development of cocaine addiction. Numerous studies have reported about the effects of cocaine on neuronal and synaptic activities in the nucleus accumbens and ventral tegmental area, which are brain regions associated with cocaine addiction; however, a li...

journal_title：Biological & pharmaceutical bulletin

authors： Sasase H,Izumi S,Deyama S,Hinoi E,Kaneda K

更新日期：2019-01-01 00:00:00

abstract：:The effect of the Dynorphin A analog, E2078, on the physicochemical properties of lipid membranes was investigated. E2078 induces the leakage of calcein, especially out of negatively-charged vesicles. The peptide binds to dipalmitoylphosphatidylglycerol sonicated vesicles according to the Lamgmuir isotherm, with a bin...

journal_title：Biological & pharmaceutical bulletin

authors： Asai Y,Watanabe S

更新日期：1999-05-01 00:00:00

abstract：:Ty2 retrotransposons are the mobile genetic elements of the yeast Saccharomyces cerevisiae. Transcriptional regulation of Ty2-917 requires a complex set of cis acting elements which are located both upstream and downstream of the transcription initiation site. Previously, the glycolysis regulatory protein Gcr1p has be...

journal_title：Biological & pharmaceutical bulletin

authors： Türkel S

更新日期：2002-09-01 00:00:00

abstract：:Two carboxylesterases with pI 6.0 and 6.2 derived from rat liver microsomes were purified. The two isozymes were remarkably different in substrate specificity, but they had equal enzymatic activity for alpha-naphthyl acetate and were inhibited equally by phenylmethylsulfonyl fluoride (PMSF) and bis-(4-nitrophenyl) pho...

journal_title：Biological & pharmaceutical bulletin

authors： Luan L,Sugiyama T,Takai S,Usami Y,Adachi T,Katagiri Y,Hirano K

更新日期：1997-01-01 00:00:00

abstract：:Using the par to rep region of the 24653 bp plasmid pN315, which is present in Staphylococcus aureus strain N315, we constructed three vectors that can be shuttled between Escherichia coli and S. aureus and maintained stably in S. aureus. Due to plasmid incompatibility, the resident plasmid in S. aureus cells can be r...

journal_title：Biological & pharmaceutical bulletin

authors： Matsuo M,Kurokawa K,Lee BL,Sekimizu K

更新日期：2010-01-01 00:00:00

abstract：:We studied nephrotoxicity following a single injection of cisplatin (CDDP) at low dose (1 mg/kg) in two groups of rats aged 52 weeks (adult, A group) and 9 weeks (young, Y group). Renal platinum (Pt) was detectable in both groups 3 h after the CDDP injection, and, from 6 h to 3 d after injection, its level in the A gr...

journal_title：Biological & pharmaceutical bulletin

authors： Namikawa K,Kinsoku A,Minami T,Okazaki Y,Kadota E,Teramura K,Hashimoto S

更新日期：1995-07-01 00:00:00

abstract：:A rat model of early sepsis induced by lipopolysaccharide (LPS) combined with interleukin-2 (IL-2) was developed. The primary aim was to assess the pharmacokinetics of gentamicin and sepsis-induced pathophysiological changes. Moreover, the effects on the glomerular filtration rate and tubular function were studied in ...

journal_title：Biological & pharmaceutical bulletin

authors： Studena S,Martinkova J,Slizova D,Krs O,Senkerik M,Springer D,Chládek J

更新日期：2012-01-01 00:00:00

abstract：:The prophylactic action of scymnol in a rat peripheral arterial occlusion model, involving injection of 5% lactic acid into the femoral artery, was investigated. Increases in serum lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities and in plasma leve...

journal_title：Biological & pharmaceutical bulletin

authors： Ishida H,Nakayasu H,Nukaya H,Tsuji K

更新日期：1999-08-01 00:00:00

abstract：:Neuroinflammation has recently been implicated as an important mechanism responsible for the progression of neurodegenerative diseases. Activated microglia produce various proinflammatory cytokines and nitric oxide (NO) that are toxic to neurons. Thus, inhibition of microglial activation may alleviate neuroinflammator...

journal_title：Biological & pharmaceutical bulletin

authors： Ock J,Hong SH,Suk K

更新日期：2010-01-01 00:00:00

abstract：:A hydroalcoholic (50%) extract of Emblica officinalis (fruit) (EO-50) reduced the severity of hepatic fibrosis induced by carbon tetrachloride (CCl4) and thioacetamide (TAA). Improved liver function was observed by measuring the levels of aspartate aminotransaminase (AST), alanine aminotransferase (ALT), alkaline phos...

journal_title：Biological & pharmaceutical bulletin

authors： Tasduq SA,Mondhe DM,Gupta DK,Baleshwar M,Johri RK

更新日期：2005-07-01 00:00:00

abstract：:Mouse thioglycollate-induced peritoneal macrophages effectively, in the absence of serum, recognized mouse polymorphonuclear leukocytes (PMNs) mildly oxidized with diamide, superoxide (hypoxanthine/xanthine oxidase) or t-butyhydroperoxide, or modified with N-ethylmaleimide (NEM). The recognition reached a maximum when...

journal_title：Biological & pharmaceutical bulletin

authors： Beppu M,Yokoyama N,Motohashi M,Kikugawa K

更新日期：2001-01-01 00:00:00

abstract：:Coenzyme Q10 (Co-Q10) is a vitamin-like supplement which appears to be safe, with minimal side effects and low drug interaction potential. Co-Q10 is used in the treatment of a variety of disorders related primarily to suboptimal cellular energy metabolism and oxidative injury. Studies supporting the efficacy of Co-Q10...

journal_title：Biological & pharmaceutical bulletin

authors： Ewees MG,Messiha BA,Abo-Saif AA,Abd El-Latif HA

更新日期：2016-01-01 00:00:00