Comparative study on recombinant chloroplastic and cytosolic ascorbate peroxidase isozymes of spinach.

abstract：:There is evidence to suggest that extracellular matrix molecules, such as proteoglycans, are involved in the regulation of mineral deposition in calcifying tissues. One mineralizing system which is characterized by extremely rapid mineralization is the hen eggshell. This eggshell consists of a pair of nonmineralized e...

journal_title：Archives of biochemistry and biophysics

authors： Arias JL,Carrino DA,Fernández MS,Rodríguez JP,Dennis JE,Caplan AI

更新日期：1992-10-01 00:00:00

abstract：:Cytochromes P450 are enzymes that catalyse the oxidation of a wide variety of compounds that range from small volatile compounds, such as monoterpenes to larger compounds like steroids. These enzymes can be modified to selectively oxidise substrates of interest, thereby making them attractive for applications in the b...

journal_title：Archives of biochemistry and biophysics

authors： Stok JE,Giang PD,Wong SH,De Voss JJ

更新日期：2019-09-15 00:00:00

abstract：:Drought is one of the most serious factors affecting crop yields in the world. Macleaya cordata (Willd.) is a draught-tolerant medicinal plant that has been proposed as a pioneer crop to be cultivated in arid areas. However, the exact molecular mechanisms through which M. cordata responds to draught stress remain elus...

journal_title：Archives of biochemistry and biophysics

authors： Yu L,Zhou L,Liu W,Huang P,Jiang R,Tang Z,Cheng P,Zeng J

更新日期：2020-05-15 00:00:00

abstract：:Previous studies in which dicyclohexylcarbodiimide (DCCD) was used to inactivate F1-ATPase enzymes have suggested that two glutamate residues in the beta-subunit are essential for catalysis. In the Escherichia coli F1-ATPase, these are residues beta-Glu-181 and beta-Glu-192. Oligonucleotide-directed mutagenesis was us...

journal_title：Archives of biochemistry and biophysics

authors： Parsonage D,Wilke-Mounts S,Senior AE

更新日期：1988-02-15 00:00:00

abstract：:The influenza A virus nonstructural protein NS1 is a multifunctional dimeric protein that acts as a potent inhibitor of the host cellular antiviral state. The C-terminal effector domain of NS1 binds host proteins, including CPSF30, and is a target for the development of new antiviral drugs. Here we present crystallogr...

journal_title：Archives of biochemistry and biophysics

authors： Xia S,Robertus JD

更新日期：2010-02-15 00:00:00

abstract：:The effect of high temperature stress on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase was examined in wheat (Triticum aestivum L.) leaves, which normally possess 46- and 42-kDa activase forms. Heat stress at 38 degrees C significantly reduced total activase mRNA levels compared ...

journal_title：Archives of biochemistry and biophysics

authors： Law RD,Crafts-Brandner SJ

更新日期：2001-02-15 00:00:00

abstract：:Intrinsically disordered proteins represent about one third of eukaryotic proteins. An additional third correspond to proteins containing folded domains as well as large intrinsically disordered regions (IDR). While IDRs may represent functionally autonomous domains, in some instances it has become clear that they pro...

journal_title：Archives of biochemistry and biophysics

authors： Arbesú M,Pons M

更新日期：2019-11-30 00:00:00

abstract：:The thermal denaturation of clathrin-coated vesicles isolated from bovine brain tissue has been studied by differential scanning calorimetry and has been compared to basket structures reformed from isolated triskelion trimers of clathrin and to isolated triskelions. The coated vesicles and reformed baskets displayed s...

journal_title：Archives of biochemistry and biophysics

authors： Schwarz FP,Steer CJ,Kirchhoff WH

更新日期：1989-09-01 00:00:00

abstract：:The effects of insulin on phospholipid metabolism and generation of diacylglycerol (DAG) and on activation of protein kinase C in rat hepatocytes were compared to those of vasopressin and angiotension II. Insulin provoked increases in [3H]glycerol labeling of phosphatidic acid (PA), diacylglycerol (DAG), and other gly...

journal_title：Archives of biochemistry and biophysics

authors： Cooper DR,Hernandez H,Kuo JY,Farese RV

更新日期：1990-02-01 00:00:00

abstract：:3-Methyl-substituted fatty acids are first oxidatively decarboxylated (alpha-oxidation) before they are degraded further via beta-oxidation. We synthesized [1-14C]phytanic and 3-[1-14C]methylmargaric acids in order to study their alpha-oxidation in isolated rat hepatocytes, rat liver homogenates and subcellular fracti...

journal_title：Archives of biochemistry and biophysics

authors： Huang S,Van Veldhoven PP,Vanhoutte F,Parmentier G,Eyssen HJ,Mannaerts GP

更新日期：1992-07-01 00:00:00

abstract：:Normal thymocytes and thymoma cells have been exposed to the toxic action of tert-butyl hydroperoxide (t-BOOH) to study the mechanisms of oxidative lethal cell injury and the sensitivity of normal and tumor cells to an oxidative stress. From our data, we can suggest that: (a) In thymoma cells two mechanisms can operat...

journal_title：Archives of biochemistry and biophysics

authors： Bartoli GM,Piccioni E,Agostara G,Calviello G,Palozza P

更新日期：1994-07-01 00:00:00

abstract：:Peroxynitrite is a strong oxidant that reacts with a variety of biomolecules in vivo and in vitro. When rat thymocytes in phosphate buffer are exposed to 25 microM peroxynitrite for 10 min, DNA single strand breaks (SSB) can be detected. These SSB are repaired if the cells are incubated in fresh media at 37 degrees C ...

journal_title：Archives of biochemistry and biophysics

authors： Salgo MG,Pryor WA

更新日期：1996-09-15 00:00:00

abstract：:Mitogens increase prostaglandin synthesis in fibroblasts. In the present studies, transcriptional activation of the prostaglandin G/H synthase isoenzyme 2 (PGHS-2) gene is shown to be responsible for this induction. Transcription of the PGHS-2 gene maximally increased within 15 min of stimulation, and elevated cellula...

journal_title：Archives of biochemistry and biophysics

authors： Evett GE,Xie W,Chipman JG,Robertson DL,Simmons DL

更新日期：1993-10-01 00:00:00

abstract：:Sphingosine-1-phosphate (SPP) acts as a first messenger in immortalized human airway epithelial cells (CFNPE9o(-)), possibly interacting with an Edg family receptor. Expression of the SPP receptors Edg-1 and Edg-3, as well as a low level of Edg-5/H218, was detected in these cells, in agreement with their ability to sp...

journal_title：Archives of biochemistry and biophysics

authors： Orlati S,Porcelli AM,Hrelia S,Van Brocklyn JR,Spiegel S,Rugolo M

更新日期：2000-03-01 00:00:00

abstract：:Properly regulated cyclin proteolysis is critical for normal cell cycle progression. A nine-amino acid peptide motif called the destruction box (D box) is present at the N terminus of the yeast mitotic cyclins. This short sequence is required for cyclin ubiquitination and subsequent proteolysis. The anaphase-promoting...

journal_title：Archives of biochemistry and biophysics

authors： Meyn MA 3rd,Melloy PG,Li J,Holloway SL

更新日期：2002-11-15 00:00:00

abstract：:Phosphoenolpyruvate (PEP) carboxylase is regulated by reversible phosphorylation in higher plants. Recently several genes encoding PEP carboxylase kinase have been cloned. The purpose of this article is to assess the contribution that information on the structure and expression of these genes is making to our understa...

journal_title：Archives of biochemistry and biophysics

authors： Nimmo HG

更新日期：2003-06-15 00:00:00

abstract：:The soluble form of mitochondrial adenosine triphosphatase was purified in an electrophoretically and immunologically pure form from sweet potato root tissue. The enzyme consisted of six kinds of subunits with different molecular weights (52,500, 51,500, 35,500, 26,000, 23,000, and 12,000), and its molecular weight wa...

journal_title：Archives of biochemistry and biophysics

authors： Iwasaki Y,Asahi T

更新日期：1983-11-01 00:00:00

abstract：:Glutamine transport in both normal and acidotic rat kidney mitochondria was investigated using both isotopic techniques and by spectroscopic measurements in which glutamine metabolism was allowed to occur. Widely used criteria for demonstrating the occurrence of carrier-mediated transport were successfully applied in ...

journal_title：Archives of biochemistry and biophysics

authors： Atlante A,Passarella S,Minervini GM,Quagliariello E

更新日期：1994-12-01 00:00:00

abstract：:The relationship between prolonged exercise, oxidative stress, and the protective capacity of the antioxidant defense system has been determined. Venous blood samples were removed from seven trained athletes before and up to 120 h after completion of a half-marathon for measurements of blood antioxidants, antioxidant ...

journal_title：Archives of biochemistry and biophysics

authors： Duthie GG,Robertson JD,Maughan RJ,Morrice PC

更新日期：1990-10-01 00:00:00

abstract：:Glycerol is a dietary component which is metabolized primarily by the liver and kidney where it is used mainly for glucose synthesis. The metabolism of glycerol is very similar to that of dihydroxyacetone which can be considered its more oxidized counterpart. The effects of these substrates on hepatic lipogenesis and ...

journal_title：Archives of biochemistry and biophysics

authors： Carmona A,Freedland RA

更新日期：1989-05-15 00:00:00

abstract：:Glucosidase I, the enzyme catalyzing the first step of N-linked oligosaccharide processing, has been purified from calf liver crude membranes [H. Hettkamp, G. Legler, and E. Bause, (1984) Eur. J. Biochem. 142, 85-90]. Binding experiments with concanavalin A-Sepharose suggest that glucosidase I is a glycoprotein with h...

journal_title：Archives of biochemistry and biophysics

authors： Schweden J,Borgmann C,Legler G,Bause E

更新日期：1986-07-01 00:00:00

abstract：:Previous experiments performed in recombinant systems have suggested that protein-protein interactions occur between the UGTs and may play a significant role in modulating enzyme activity. However, evidence of UGT protein-protein interactions either in vivo or in more physiologically relevant in vitro systems has yet ...

journal_title：Archives of biochemistry and biophysics

authors： Konopnicki CM,Dickmann LJ,Tracy JM,Tukey RH,Wienkers LC,Foti RS

更新日期：2013-07-15 00:00:00

abstract：:Three heme-containing proteins of M(r) 57,000, 46,000, and 42,000 have been isolated from microsomes of elicitor-treated suspension-cultured cells of French bean. Following purification to homogeneity they were shown to be distinct with respect to amino acid and sugar compositions. The M(r) 57,000 protein proved to be...

journal_title：Archives of biochemistry and biophysics

authors： Rodgers MW,Zimmerlin A,Werck-Reichhart D,Bolwell GP

更新日期：1993-07-01 00:00:00

abstract：:Epidemiological evidence strongly suggests that lycopene consumption contributes to prostate cancer risk reduction. Preclinical studies show that lycopene acts via different mechanisms, which have the potential to cooperate in reducing the proliferation of normal and cancerous prostate epithelial cells, in reducing DN...

journal_title：Archives of biochemistry and biophysics

authors： Wertz K,Siler U,Goralczyk R

更新日期：2004-10-01 00:00:00

abstract：:The activities, properties, and steady-state kinetics of the five enzymes catalyzing the synthesis of 1-acyl- and 1-alkyl-sn-glycerol 3-phosphate in the cultured skin fibroblasts from Zellweger syndrome patients and normal controls were studied in detail. Judging from their Km and Vmax values, glycerol phosphate acylt...

journal_title：Archives of biochemistry and biophysics

authors： Webber KO,Datta NS,Hajra AK

更新日期：1987-05-01 00:00:00

abstract：:Laser Raman spectroscopy has been applied to native and dithiothreitol-treated bovine cortical gamma II crystallin to examine the state of the thiol groups and the presence of a putative disulfide bridge. The data reveal significant differences in two key spectral regions. In the thiol stretching region (2500-2600 cm-...

journal_title：Archives of biochemistry and biophysics

authors： Pande J,McDermott MJ,Callender RH,Spector A

更新日期：1989-02-15 00:00:00

abstract：:Liver mitochondria provided with an oxidizable substrate, ATP, oxygen, and an ADP-generating system (soluble F1-ATPase) were used to reevaluate the rate-controlling step(s) intrinsic to all of the processes of mitochondrial oxidative phosphorylation. The quantity termed "control strength" (C), previously defined as th...

journal_title：Archives of biochemistry and biophysics

authors： Davis EJ,Davis-Van Thienen WI

更新日期：1984-09-01 00:00:00

abstract：:The effects of nalidixic acid and hydroxyurea on nuclear and chloroplast DNA formation in cultured cells of Nicotiana tabacum were investigated. At low concentrations (5 and 20 micrograms/ml) nalidixic acid, an inhibitor of DNA gyrase, exhibited a greater inhibitory effect on plastid DNA synthesis than on nuclear DNA ...

journal_title：Archives of biochemistry and biophysics

authors： Heinhorst S,Cannon G,Weissbach A

更新日期：1985-06-01 00:00:00

abstract：:Protein kinase CK2 of Candida albicans has been purified to near homogeneity by a procedure which involves chromatography on DEAE-cellulose, phosphocellulose, Q-Sepharose, and heparin-agarose. The purified enzyme has the characteristic properties of animal and yeast CK2, i.e., it utilizes ATP as well as GTP as phospha...

journal_title：Archives of biochemistry and biophysics

authors： Walz K,Pardo PS,Passeron S

更新日期：1997-04-15 00:00:00

abstract：:alpha1 Antitrypsin (alpha1AT) is the archetypal member of the serpin superfamily. Current knowledge of its inhibitory mechanism does not provide for any heparin-induced enhancement of serine proteinase inhibition. Since previous results have shown that an apparently altered alpha1AT form may be purified from the plasm...

journal_title：Archives of biochemistry and biophysics

authors： Finotti P,de Laureto PP

更新日期：1997-11-01 00:00:00