Abstract:
:Megakaryocyte polyploidization is an advantageous and regulated mechanism which leads to an increase in platelet production. In megakaryocyte cell lines, polyploidization can be obtained by using cytochalasin B, an inhibitor of the actin polymerization. The Nucleolar Organizer Regions (AgNORs) are parts of nucleolar DNA transcribed into ribosomal RNA. They are detected by silver staining technique and their number is proportional to protein synthesis. In order to estimate protein synthesis in polyploidizing megakaryocytes, AgNORs were measured in three cell lines with megakaryocyte properties (DAMI, HEL and K562) after a 4-day culture in the presence or absence of cytochalasin B, an inhibitor of the actin polymerization. The mean number of AgNORs per cell was 16.4 +/- 4.3 (m +/- SEM); 24.4 +/- 2.5 and 13.6 +/- 3.1 for DAMI, HEL and K-562 cell lines, respectively. The addition of cytochalasin B (2 micrograms/ml) increased significantly the number of AgNORs per cell (DAMI: 437%, HEL: 384% and K-562: 345% of controls, p < 0.05 by t-test). Moreover, the numbers of nucleoles per cell after addition of cytochalasin B were augmented significantly (DAMI: 258%, HEL: 271% and K-562: 264% of controls, p < 0.05 by t-test). The total protein content estimated by Bradford's method increased significantly to 938%, 326% and 388% of controls in DAMI, HEL and K562, respectively (p < 0.05 by t-test) in cells where actin was inhibited by cytochalasin B. In the presence of cytochalasin B, the endomitotic index (EI) [mean of (log2 DNA content expressed in N) 1] measured by flow cytometry increased to 368%, 207% and 538%, for DAMI, HEL and K-562 cell lines, respectively (p < 0.05 by t-test) after treatment with cytochalasin B. In contrast, the number of AgNORs per unit of DNA (EI) and the total protein content per unit of DNA did not change for DAMI, HEL and K-562 cell lines (p < 0.05 by t-test) after treatment with cytochalasin B. In conclusion, the increase in the number of the Nucleolar Organizer Regions by an agent known to stimulate polyploidization of megakaryocytic cell lines suggests that polyploidization occurs by enhanced protein production proportionally to DNA synthesis.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Baatout S,Chatelain B,Staquet P,Symann M,Chatelain Csubject
Has Abstractpub_date
1998-01-01 00:00:00pages
459-64issue
1Aeissn
0250-7005issn
1791-7530journal_volume
18pub_type
杂志文章abstract::Proteoglycan epitopes recognized by poly- or monoclonal antibodies were studied at the cell surface of 3LL murine tumor cell lines with different metastatic capacity. A decreased expression of heparan sulphate and chondroitin sulphate core protein epitopes was observed in the highly metastatic variant HM compared to t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract:BACKGROUND/AIM:Perioperative intra-abdominal infection has been reported as a risk factor for metastasis. The aim of this study was to investigate the mechanism by which peritonitis induces immunosuppression in the lung, which in turn promotes lung metastasis. MATERIALS AND METHODS:C57BL/6 mice were intravenously admi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12733
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:Cyclooxygenases (COXs) and prostanoids play pivotal roles in colon carcinogenesis. This study was designed to determine the chemopreventive effects of ONO-8711, a selective prostaglandin E receptor EP1 antagonist, on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in male F344...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Recent results concerning new antitumor metallocene derivatives have shown that not only numerous titanocene dihalides and bis(pseudohalides) exhibit antiproliferative activity, but that also some titanocene complexes (C5H5)2TiX2 containing carboxylates or hydrochlorinated aminothiophenolates as ligands X, are charact...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract:BACKGROUND:Cyclin D1 is a key protein involved in cell cycle regulation. A common A870G single nucleotide polymorphism in exon 4 of the cyclin D1 gene (CCND1) has an effect on the transcription of two different cyclin D1 mRNAs. Association of the genetic polymorphism of A870G of CCND1 with several cancer risks has been...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Cisplatin is one of the most widely used and effective chemotherapeutic agents for the treatment of several human malignancies. Although the effectiveness of cisplatin is high, its toxicities justify the demand for improved formulations of this drug. A liposomal formulation of cisplatin, Lipoplatin, was developed in o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
abstract::Consumption of dietary fat has been linked to the high incidence of certain cancers. However, recent research has stimulated interest in conjugated linoleic acid (CLA), a newly recognized anticarcinogenic fatty acid. Human MCF-7 breast cancer cells were incubated for 12 d in culture medium supplemented with various co...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::Analysis of the overall patterns of gene expression at the polypeptide synthesis level has revealed two S-phase specific, proliferation sensitive nuclear proteins [cyclin (PCNA) and dividin (phosphoprotein)] whose rate of synthesis increases at the G1/S transition border of the cell cycle. All available evidence sugge...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:The optimal palliative chemotherapy in endometrial cancer is unknown. CASE REPORT:A patient was diagnosed to have primary endometrioid endometrial cancer metastatic to the pelvic, paraaortic and right inguinal lymph nodes (FIGO IVb). After radical surgery, she received 6 adjuvant cycles of cisplatin and epi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract::In the colon of F344 rats treated with 2 x 15 mg/kg body weight of azoxymethane (AOM), the density (number of lesions/cm2/rat) of flat aberrant crypt foci (ACF) was 13-fold higher (p < 0.05) in the surface area of mucosa immediately adjacent to lymphoid follicles compared with the density of these lesions in the rest ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Cell proliferation measurements using bromodeoxyuridine administration to patients and flow cytometry to measure Tpot in head and neck cancer are discussed. Several hundred patients have now been assessed and the majority of studies suggest that this group of tumours represent a relatively fast proliferating site with...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1998-11-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is the sixth most common malignancy in humans including type I diabetic and normal rats. Tobacco and alcohol, as well as dysregulation of oncogenes and tumor suppressor genes, epigenetic changes and mitochondrial mutations have been implicated in OSCC development. Recent epidemiolog...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2007-11-01 00:00:00
abstract::Explantation and cultivation in vitro of 55 human biopsies of histologically normal urothelial tissue, and 63 biopsies from transitional cell carcinomas (TCC) revealed a primary success rate of about 75% and a secondary success rate of about 50% with no significant differences, between normal and TCC derived cultures....
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-09-01 00:00:00
abstract:BACKGROUND:The safety and advantages of perioperative autologous blood transfusion (ABT) were evaluated on hepatectomy for hepatocellular carcinoma (HCC). MATERIALS AND METHODS:Blood samples were obtained and stored from 30 patients with HCC. HCC cells were investigated by the presence of AFPmRNA using RT-PCR after st...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract:BACKGROUND/AIM:Vanek's tumor is an inflammatory fibroid polyp (IFP) of very low incidence, which originates in the submucosa of the stomach, duodenum, jejunum and ileum. The etiology of this tumor is still unknown. Histologically, IFP is characterized by submucosal spindle cell proliferation in fibromyxoid stroma with ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-12-01 00:00:00
abstract::In the study of 65 matched pairs of lung tumor tissue and normal lung parenchyma, cathepsin B (CB) activity was found to be increased about 4.6-fold, when regarding median levels. CB activity was found to be insignificantly higher in adenocarcinoma compared to the other histologic cell types. CB levels did not correla...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Hepatocellular carcinoma is one of the most common malignancies in the world, with the liver being the second most frequently involved organ in metastatic disease. Although the gold standard treatment for malignant liver disease is surgical resection, only few patients can undergo such an intervention. This explains t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:For eradicating portal venous tumor extension and intrahepatic metastasis in hepatocellular carcinoma (HCC), anatomical resection is, in theory, preferable. PATIENTS AND METHODS:We carried-out a retrospective cohort study in 110 patients who underwent curative hepatic resection (anatomical resection; n=20, ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
abstract::Leukemia inhibitory factor (LIF) is prevalently expressed in human medulloblastomas in vivo and in vitro. Blockage of LIF expression or biological function can inhibit in vitro growth and induce differentiation-like phenotypes of medulloblastoma cells (Med-3), suggesting a redifferentiation potential of Med-3 cells wh...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::The effect of selenite on mitochondrial morphology, selenoprotein synthesis, membrane potential, phosphorylating respiration, P/O ratio and respiratory control index were evaluated relative to selenite inhibition of DNA synthesis in murine mammary epithelial cell lines. No inhibition of mitochondrial function was note...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::We report application of a novel immunohistochemical procedure for the staining of apoptotic (AP) cells in paraffin sections using monoclonal antibody (MAb) to single-stranded DNA. MAb differentiated between apoptosis and necrosis and in contrast to in situ end labelling specifically stained only AP cells. AP carcinom...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Signaling between androgen receptor (AR) and mTOR may be crucial for prostate cancer cells to endure the low androgen and suboptimal nutrient conditions produced by androgen deprivation therapy. MATERIALS AND METHODS:AR and mTOR cross-talk was examined in LNCaP cells exposed to either high or low testostero...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
abstract::Methodological aspects of testing chemosensitivity by the 6-day subrenal capsule (SRC) assay in immunocompetent mice were investigated. Human tumor xenografts, serially transplanted in athymic nude mice, were used as source of material. All drugs were given by the intravenous route. Administration of the drugs on days...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND/AIM:To investigate whether high-dose-rate (HDR) intracavitary brachytherapy (IBT) dose ratios can predict treatment outcomes in patients with stage IIB cervical cancer. PATIENTS AND METHODS:Ninety-three patients treated with weekly cisplatin-based concurrent chemoradiotherapy and HDR IBT were analyzed. Pote...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11325
更新日期:2017-01-01 00:00:00
abstract::We have studied the effects of PAI-1 on the formation of metastasis using human fibrosarcoma cells and DNA transfection. A clone (1-3C), which shows low constitutive expression of PAI-1 and low metastatic potential to the lung, was selected from human fibrosarcoma cell line HT-1080. Newly-derived clones transfected wi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Lipomas are the most common benign tumors of the mesenchyme; they are composed of mature lipocytes. Benign fatty tumours can arise in any location in which fat is present. Most patients affected by such tumours are in their fifth or sixth decade of life. Only rarely are children affected. Lipomas may be single or mult...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Bone lesions in multiple myeloma (MM) are screened with radiological skeletal survey (RSS) due to its widespread availability. Although bone lesions can be missed by RSS, more sensitive radiological surveys are not as yet recommended for routine use due to the low availability of the methodology and economic...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Concurrent chemoradiotherapy with gemcitabine improves median survival for patients with unresectable pancreatic cancer. Recently, hyperfractionated accelerated radiotherapy (HART) has been used to treat these patients; however, the safety and efficacy are not well defined. PATIENTS AND METHODS:The standard...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Initial U.S.A. breast cancer screening rates have risen, but not repeat screening, especially among low-income minority populations. Latinas are particularly at-risk of underscreening. Consequently, late-detection is common, with increased risk of dying after diagnosis. Why women with low-income, particularl...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::The influence of human stromal fibroblasts cultured from normal, non-malignant and malignant breast tissues on in vivo growth of human breast tumour cells was studied. Earlier appearance and increased incidence were observed when low cell inocula of breast MDA-MB 231 tumour cells were coinjected with fibroblasts from ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00