Abstract：
:A general spectroscopic method is described that might be applied to validating amino acid sequences in peptides and protein fragments with a view to it becoming a routine procedure with which to characterize biotechnology drug products. The tripeptides are the L-enantiomers of GGA, GGH, GGI, GGL, GGF, GHG, LGG, and YGG. The simple procedure calls for their complexation with Cu(II) ion in strong aqueous base. Binding the first three residues in the sequence, beginning at the amine terminus, completes the coordination sphere of the Cu(II) ion, so duplication of the initial sequence from peptide to peptide could be an important limiting factor in determining the extent of differentiation that is possible. The analytical focus is the selectivity associated with the chirality properties of the peptides. Detection is by circular dichroism operating in the visible range. The eight analytes were chosen as representative of a series where the sequences are most similar and therefore potentially the most difficult to discriminate spectroscopically. All have just one chiral center. Using ellipticity data at all (n = 1500) wavelengths in the measured spectra, and two novel data reduction procedures, total discrimination among all eight analytes is achieved. The method has considerable potential for use in quality control of peptide and protein biotechnological drug forms, especially their enantiomeric purities.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Purdie N,Province DW,Johnson EAdoi
10.1021/js980393osubject
Has Abstractpub_date
1999-07-01 00:00:00pages
715-21issue
7eissn
0022-3549issn
1520-6017pii
S0022-3549(15)50829-0journal_volume
88pub_type
杂志文章abstract：:A suspension of micronized prednisolone acetate was separated into four fractions by the technique of centrifugal elutriation. Data showed that each fraction had a narrow particle size. The dissolution experiments were carried out under sink conditions (less than 10% of saturation concentration) in a dissolution appar...
journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600720225
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abstract：:A radioimmunoassay for the quantitation of meobentine sulfate, a novel antidysrhythmic and antifibrillatory agent in biological fluids, is described. Antisera were raised in rabbits in response to immunization with a conjugate of bovine serum albumin and a meobentine analog with a propionic acid sidechain ortho to the...
journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.2600710615
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journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.20403
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doi：10.1002/jps.22349
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600750713
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journal_title：Journal of pharmaceutical sciences
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journal_title：Journal of pharmaceutical sciences
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更新日期：1979-04-01 00:00:00
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更新日期：2017-01-01 00:00:00
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更新日期：2001-09-01 00:00:00
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更新日期：2016-10-01 00:00:00
abstract：:There is a need to evaluate the permeability of human ulcerated tissue and periulcer tissue in order to assess the possible treatment of such a localized pathological lesion with a topical therapy. In vitro percutaneous absorption studies were undertaken to evaluate an animal model that may mimic this clinical situati...
journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1021/js970159i
更新日期：1997-12-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.2600830721
更新日期：1994-07-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.22232
更新日期：2010-09-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.22695
更新日期：2011-11-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.21676
更新日期：2009-09-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.21939
更新日期：2010-04-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600660620
更新日期：1977-06-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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doi：10.1016/j.xphs.2017.03.003
更新日期：2017-09-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.2600700110
更新日期：1981-01-01 00:00:00
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doi：10.1002/jps.2600740509
更新日期：1985-05-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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更新日期：2012-12-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600750205
更新日期：1986-02-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.24412
更新日期：2015-05-01 00:00:00
abstract：:The estimation of aqueous solubilities of organic nonelectrolytes by the General Solubility Equation (GSE) as proposed by Valvani and Yalkowsky (1980) is used in this study. The data and assumptions on which the GSE are based are reevaluated, and the equation is revised. The revised GSE is validated on a set of 580 ph...
journal_title：Journal of pharmaceutical sciences
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doi：10.1002/1520-6017(200102)90:2<234::aid-jps14>3.0.c
更新日期：2001-02-01 00:00:00
abstract：:Several factors affecting the dissolution rate of cholesterol in monooctanoin were investigated. This solvent is used clinically for dissolution of residual cholesterol gallstones in the bile duct after cholecystectomy. The effect of added water on dissolution rate, measured using the static- or rotating-disk methods,...
journal_title：Journal of pharmaceutical sciences
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doi：10.1002/jps.2600730711
更新日期：1984-07-01 00:00:00
abstract：:Stirred media milling is the main technology for producing colloidal nanocrystalline suspensions. A number of studies have been reported on the effect of different operating parameters for lab, pilot, and industrial scales. However, typical milling tool box that can be used to support candidate from selection up to ph...
journal_title：Journal of pharmaceutical sciences
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更新日期：2017-07-01 00:00:00
abstract：:The aim of this work was to evaluate the low molecular weight chitosans (LMWCs) as enhancers of transdermal administration of baicalin, an useful drug for the treatment of atopic dermatitis, viral hepatitis, and HIV infection. Permeation experiments were performed in vitro through mouse skin by using Franz cells. Impr...
journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.22063
更新日期：2010-07-01 00:00:00
