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Cytotoxicity resulting from addition of HIV-1 Nef N-terminal peptides to yeast and bacterial cells.

Abstract：

:The Nef protein of human immunodeficiency type 1 (HIV-1) has been implicated in diverse intracellular functions; however, extracellular functions have been less studied. Nef and the N-terminus of Nef possess membrane-perturbing and fusogenic activities in artificial membranes that also cause cytotoxicity to human cells, including lymphocytes. The present study investigates the toxicity of HIV-1 Nef peptides employing yeast and bacterial cells. The N-terminal portion of Nef was found to cause cell killing in Escherichia coli and in a variety of yeast cells. This activity was enhanced by myristylation of the Nef N-terminus, a modification that did not lead to toxicity in a control peptide. Cell death in yeast was due to permeabilization of the cell membrane as determined by the propidium iodide uptake of peptide-treated cells. Extracellular Nef, or its breakdown products, may have effects similar to the Nef peptides described here and could be responsible, at least in part, for the death of cells in lymphoid tissues during AIDS. Assays using yeast or bacteria are convenient, inexpensive, and robust and should be useful in further analysis and screening of inhibitors of this activity associated with HIV-1 Nef.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Macreadie IG,Lowe MG,Curtain CC,Hewish D,Azad AA

doi

10.1006/bbrc.1997.6349

subject

Has Abstract

pub_date

1997-03-27 00:00:00

pages

707-11

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(97)96349-X

journal_volume

232

pub_type

杂志文章

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