Abstract:
:Embedment-free electron microscopy (EFEM) is a new method which allows the visualisation of cytoskeleton in whole-mounted cells. In this study we employed EFEM to investigate the structure of cellular scaffolds in glioma C6 cell line. The cells were extracted with Triton X-100 that dissolves phospholipids in the membranes and removes most of cytoplasmic soluble proteins. The DNA and nuclear histones were removed with DNase I and high-salt buffer, respectively. The remaining cellular frameworks were temporary embedded in diethylene glycol distearate (DGD), sectioned and observed in transmission and scanning electron microscope after the removal of DGD. The predominant structure was the extensive meshwork of 10-20 nm filaments in the cytoplasm (cytomatrix) and 15-30 nm filaments in the nucleus (nuclear matrix). The 5 nm filaments, presumably corresponding to the actin filaments, were present in the cytomatrix, but not in the nuclear matrix. Moreover, the ultrathin (3 nm) filaments, connecting other cytoskeletal components were detected. Those are possibly identical with the previously described plectin filaments. For the first time we report the occurrence of ultrathin filaments in the nuclear matrix. Thus, in a addition to the well known cytoskeletal components (microtubules, intermediate filaments, actin microfilaments) EFEM showed a new type of filaments (the ultrathin filaments) in the cytomatrix and nuclear matrix. Further immunocytochemical studies are needed to determine the biochemical identity of the filaments observed in EFEM.
journal_name
Acta Neurobiol Exp (Wars)journal_title
Acta neurobiologiae experimentalisauthors
Gajkowska B,Cholewiński M,Gniadecki Rsubject
Has Abstractpub_date
2000-01-01 00:00:00pages
147-58issue
2eissn
0065-1400issn
1689-0035journal_volume
60pub_type
杂志文章abstract::The term reflex is used in its widest sense and denotes the neural processes and effector responses controlled by the evoking stimulus and the feedback stimuli. The reflexes having their specific component facilitated by drive central processes are denoted as higher. Escape, avoidance and approach higher reflexes are ...
journal_title:Acta neurobiologiae experimentalis
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doi:
更新日期:1972-01-01 00:00:00
abstract::Two experiments involving parallel procedures to investigate stimulus generalization in prefrontal dogs under alimentary and defensive reinforcement were compared. Twelve dogs in the alimentary study were trained on a 50 percent partial reinforcement schedule, and 24 dogs were trained to avoid shock with either contin...
journal_title:Acta neurobiologiae experimentalis
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更新日期:1982-01-01 00:00:00
abstract::The relationship between neocortical high voltage spindle activity (HVS) and the level of arousal (state of vigilance) was investigated in imp-DAK rats. Two groups of single records (6 h and 24 h) and one group of repeated ones (6 h daily for three consecutive days) were analysed. Five levels of arousal (states) were ...
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更新日期:1994-01-01 00:00:00
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更新日期:1997-01-01 00:00:00
abstract::Fifteen laboratory born cats were used: seven binocularly deprived, four reared under condition of daily alternating monocular exposure and four controls. At the 7th month of age four of the binocularly deprived cats and all the remaining cats were trained monocularly in a two choice apparatus. In the first stage of t...
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更新日期:1986-01-01 00:00:00
abstract::Parkin and alpha-synuclein (α-syn) are two key proteins involved in the pathophysiology of Parkinson's disease (PD). Oligomerization/aggregation and excessive secretion of α-syn contributes to PD through free radical stress, mitochondrial impairment, and synaptic dysfunction. Parkin, an E3 ubiquitin ligase, is conside...
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pub_type: 杂志文章,评审
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abstract::In an effort to determine the plasticity of the proliferative matrix of the hippocampus in an adult rat to environmental manipulation, the following study was conducted. Fifteen adult male Long-Evans rats (6 months old at the start of the experiment) were placed into two different environments for 3 months. Seven anim...
journal_title:Acta neurobiologiae experimentalis
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更新日期:1982-01-01 00:00:00
abstract::The neurocognitive consequences of correct or incorrect spatial prediction in a sequential S1-S2 paradigm were assessed. Sequential dependence on previous trial outcome (valid or invalid) was assessed by late Event-Related Potentials (ERPs) and behavioral responses. Two different experiments were performed, situating ...
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更新日期:2009-01-01 00:00:00
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doi:
更新日期:2010-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章,评审
doi:
更新日期:1994-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
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更新日期:2013-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The registration of the step phases and the relations between them for all of the four limbs were described in the amygdalar, rubral and funicular cats. The evident changes in motor behavior observed after damage of the amygdala and red nucleus did not result from coordination disorders. Conversely, the damage of the ...
journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Time characteristics of lateral-hypothalamic self-stimulation were analyzed in rats using point process statistics. Inter-barpressing interval distributions were usually unimodal and asymmetrical and resembled shifted exponential distribution, i.e. bar-pressings seem to be performed more or less randomly over time. Wh...
journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Disturbances of enteric nerve-mediated anorectal evacuation mechanisms have medical and social impact. The study aimed at further eliciting the contribution of cholinergic and nitrergic neurotransmission systems to modular nerve networks in different regions of Wistar rat anorectum. Electrical field stimulation (EFS, ...
journal_title:Acta neurobiologiae experimentalis
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doi:
更新日期:2012-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1974-01-01 00:00:00
abstract::A review of recent anatomical evidence shows that every neocortical area and many nuclei from the dorsal thalamus project to restricted neostriatal regions. The neurobehavioral literature offers many examples of the same results obtained with lesions, stimulations or recordings in a neocortical area and the associated...
journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1972-01-01 00:00:00
abstract::Eight cats were binocularly deprived of pattern vision by rearing in masks from the time of eye opening. Twenty five groups of 3 neurons and 28 neuronal pairs were studied in visual orientation columns of their striate cortices. The crosscorrelograms of neuronal discharges were analyzed and the inference of underlying...
journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
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doi:
更新日期:1989-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
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doi:
更新日期:2018-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1973-01-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
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doi:
更新日期:1991-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::The effect of sphingosine on intracellular calcium signalling in glioma C6 cells was studied with Fura-2 video imaging technique. Sphingosine had a direct effect on changes in cytosolic Ca2+ concentration only when applied at high concentration of 100 microM, causing the cytosolic Ca2+ level to rise. However, at a muc...
journal_title:Acta neurobiologiae experimentalis
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00