Abstract：
OBJECTIVE:Human trophoblast proteolytic activity is believed to have implications for early implantation events and maintenance of chorionic structural integrity later in gestation. Abnormal release of chorion-derived extracellular matrix proteins such as fibronectin may identify patients at risk for preterm labor and delivery. The aim of this study was to characterize the enzyme(s) potentially responsible for trophoblast-mediated proteolysis of fibronectin. STUDY DESIGN:Human term cytotrophoblasts were analyzed for their capacity to cleave fibronectin into discrete proteolytic fragments. Selective protease inhibitors were used to characterize trophoblast-derived enzymes with fibronectinase activity. Analysis and quantitation of fibronectin fragment release was determined by Western immunoblots and enzyme-linked immunosorbent assays. RESULTS:Fibronectinase activity in trophoblast cultures was found to be both cell mediated and secreted, with the release of discrete fibronectin fragments into the media. Cell-mediated proteolytic activity could be partially inhibited by serum, whereas conditioned media containing fibronectinase activity was completely inhibited by serum, a serine protease inhibitor, and a selective inhibitor of urokinase-type plasminogen activator. Digestion of fibronectin with pure urokinase produced a similar pattern of fibronectin fragments compared with fibronectinase-generated fragments. Immunodepletion of urokinase from trophoblast media abolished fibronectinase activity. CONCLUSIONS:Trophoblast-derived urokinase-type plasminogen activator has significant proteolytic activity in vitro with the capability of cleaving fibronectin into discrete fragments. In early pregnancy this activity could be part of the enzymatic cascade leading to uterine extracellular matrix remodeling and implantation. Later in pregnancy trophoblast derived urokinase could promote normal or inflammation-induced changes in the chorionic extracellular matrix.
journal_name
Am J Obstet Gynecoljournal_title
American journal of obstetrics and gynecologyauthors
Monzón-Bordonaba F,Wang CL,Feinberg RFdoi
10.1016/s0002-9378(97)80012-9subject
Has Abstractpub_date
1997-01-01 00:00:00pages
58-65issue
1 Pt 1eissn
0002-9378issn
1097-6868pii
S0002937897706501journal_volume
176pub_type
杂志文章abstract：:A retrospective study of 56 patients treated for carcinoma in situ of the vulva over a 24-year period has shown a relative increase in the occurrence of this neoplasm. Only 6.7% of patients in the first 14 years of the study period were less than 35 years of age, as compared with 29.3% in the last decade. In the earli...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(84)90669-0
更新日期：1984-11-15 00:00:00
abstract：:Intrapartum fetal scalp blood pH studies remain controversial in obstetrics. Electronic fetal monitoring, by contrast, has achieved nearly uniform acceptance. Controlled comparative studies from one institution are unlikely to provide adequate numbers. Intrapartum fetal monitoring is used consistently at our instituti...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(84)90233-3
更新日期：1984-06-01 00:00:00
abstract：:Bleeding from the site of cordocentesis can be detected by ultrasound examination, but significant hemorrhage into the amniotic fluid rarely occurs. To evaluate the relative contribution of amniotic fluid thromboplastins and the quantity of Wharton's jelly in facilitating coagulation at the puncture site, amniotic flu...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(89)90464-x
更新日期：1989-02-01 00:00:00
abstract：:We report 2 cases of 47,XXX that were diagnosed prenatally and were screened positive for trisomy 21 by biochemical and ultrasound markers. These cases underline the importance of discussing the sex chromosome abnormalities during the genetic counseling after an abnormal triple screen test or ultrasound examination. ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2004.12.037
更新日期：2005-05-01 00:00:00
abstract：:Antisperm cell-mediated immunity has been associated with infertility in men and women. The purpose of this study was to determine whether the products of activated lymphocytes and macrophages (lymphokines and monokines) affect the fertilizing ability of human spermatozoa in the zona-free hamster egg penetration test....
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(89)90179-8
更新日期：1989-05-01 00:00:00
abstract：:Amide-linked local anesthetic agents, such as lidocaine and bupivacaine, can become "trapped" in their ionized forms on the fetal side of the placenta, and therefore their net transfer across the placenta is increased. An ester-linked local anesthetic agent, 2-chloroprocaine, is rapidly metabolized, and placental tran...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(85)90295-9
更新日期：1985-02-01 00:00:00
abstract：:In Finland an organized mass screening program covers the total female population. Based on the material (429,832) from a nationwide mass screening registry it was estimated that the female population not subjected to electrocoagulation of the uterine cervix (362,511) had a sixfold risk of frankly invasive carcinoma, ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(78)90115-1
更新日期：1978-07-01 00:00:00
abstract：OBJECTIVE:Our aim was to determine the ability of pathogens commonly associated with genital tract infection and preterm labor to incorporate arachidonic acid and to metabolize it to prostaglandins or to other eicosanoids. STUDY DESIGN:Four common genital tract pathogens, Escherichia coli, Streptococcus viridans, Bact...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(92)91631-j
更新日期：1992-05-01 00:00:00
abstract：:During a 40 year period 374 patients with infiltrative carcinoma of the vulva were evaluated. Some form of radical surgery was the treatment chosen for 278 patients. The absolute 5 year survival rates for all clinical stages treated with some form of radical surgery was 66.8 per cent with a corrected rate of 73.9 per ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(16)33392-0
更新日期：1976-04-15 00:00:00
abstract：:There are conflicting data in the literature concerning the effects of oral contraceptives on platelet aggregation. We studied 26 healthy women who used oral contraceptives for at least three months. The control group consisted of 25 healthy women of childbearing age, who were receiving no medications. No differences ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(78)90897-9
更新日期：1978-10-01 00:00:00
abstract：OBJECTIVE:To characterize the serum metabolome of a primate model of in utero high-fat exposure. STUDY DESIGN:Serum from maternal and fetal (e130) macaque monkeys exposed to either a high-fat or control diet were analyzed by gas chromatography-mass spectrometry. Multivariate data analysis was performed to reduce the g...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2009.06.041
更新日期：2009-09-01 00:00:00
abstract：:Pancervicovaginal (Papanicolaou) smears exhibiting pseudomycilial-like clumps of organisms obtained from 35 women employing IUD's were studied by direct immunofluorescent technique for identification of A. israelii and A. naeslundi. In every case the specific fluorescence was achieved with species-specific antiserum a...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(78)90604-x
更新日期：1978-06-01 00:00:00
abstract：:The results of preoperative computed tomography and operative findings were assessed retrospectively in 52 patients with cervical, uterine, and ovarian neoplasms. Overall sensitivity and specificity for all disease states was 57% and 79%, respectively. Overall diagnostic accuracy was 69%. The use of computed tomograph...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(86)90325-x
更新日期：1986-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to show and discuss an association between fetal inflammatory response syndrome (FIRS) and an adverse neonatal outcome defined as combined severe neonatal morbidity and mortality in preterm neonates hospitalized in our neonatal intensive care unit. STUDY DESIGN:This was an observati...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2013.08.030
更新日期：2013-12-01 00:00:00
abstract：:Experiments were performed in conscious rabbits to investigate when during pregnancy cardiac baroreceptor reflex gain decreases. A decrease in gain was first observed after 3 weeks of gestation (-34.1 +/- 4.9 to -15.7 +/- 3.1 beats/min/mm Hg, p < 0.05) and became more pronounced after 4 weeks (-30.2 +/- 4.7 to -8.6 +/...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(97)70571-4
更新日期：1997-03-01 00:00:00
abstract：:Chlamydia trachomatis is becoming increasingly known for its ability to cause severe injury to the female genital tract. Little investigation has been done in its role in diseases of Bartholin's glands. We present what we believe to be the first case report of an abscess of a Bartholin's gland in which Chlamydia trach...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：OBJECTIVE:Our purpose was to describe the techniques we have developed to remove nonpalpable subcutaneous and intramuscular Norplant capsules with the patient under local anesthesia and with use of real-time ultrasonographic guidance in an office setting. STUDY DESIGN:This was a noncomparative, descriptive study of ou...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(98)70321-7
更新日期：1998-06-01 00:00:00
abstract：:This modification of Gustavii's hypothesis recognizes the central role of saline-induced decidual cell necrosis and secondary prostaglandin release in the initiation of myometrial activity. In the absence of significant necrosis in the decidua basalis, passive stretching of the subplacental myometrium is considered to...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(76)90693-1
更新日期：1976-12-15 00:00:00
abstract：:With the establishment of a normal range of values for the growth of the ultrasound fetal femur length, a new approach for the study of growth-retarded fetuses is suggested. Ultrasound femur length was measured on a group of "high-risk" pregnancies, and its ability to differentiate the symmetrically small from the asy...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(82)90390-8
更新日期：1982-09-01 00:00:00
abstract：OBJECTIVE:The molecular mechanisms by which maternal diabetes impairs embryogenesis are not established. This study aimed to determine the developmental genes and molecular pathways that are involved in diabetic embryopathy, by comparing gene expression profiles in the yolk sacs between the embryos of diabetic and cont...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2006.05.054
更新日期：2006-10-01 00:00:00
abstract：BACKGROUND:Several articles have implied that progestogen supplementation during pregnancy to reduce the risk of preterm birth may increase the risk for developing gestational diabetes mellitus. OBJECTIVE:The purpose of the present meta-analysis was to accumulate existing evidence concerning this correlation. DATA SO...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,meta分析
doi：10.1016/j.ajog.2019.05.033
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVE:Fluctuations in sex hormones can trigger angioedema attacks in women with hereditary angioedema. Combined oral contraceptive therapies, as well as pregnancy, can induce severe attacks. The course of angioedema may be very variable in different women. STUDY DESIGN:Within the PREHAEAT project launched by the E...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2008.04.034
更新日期：2008-11-01 00:00:00
abstract：:Puerperal ovarian vein thrombosis is a dangerous complication of childbirth and often leads to inferior vena cava thrombosis and multiple pulmonary emboli. Computed tomography of the abdomen is useful in early diagnosis. Two patients with typical computed tomographic features are presented. ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(88)80168-6
更新日期：1988-10-01 00:00:00
abstract：:Progesterone is essential for the maintenance of pregnancy. Several small trials have suggested that progesterone supplementation may reduce the risk of miscarriage in women with recurrent or threatened miscarriage. Cochrane Reviews summarized the evidence and found that the trials were small with substantial methodol...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/j.ajog.2019.12.006
更新日期：2020-08-01 00:00:00
abstract：:Serum corticosteroid-binding globulin (CBG) concentrations in blood respond to endogenous or exogenous estrogens in a dose-response manner and serve as a biologic assay for estrogens. Because endometrial carcinoma is associated with unopposed or excessive estrogen stimulation, the CBG binding capacity of sera of patie...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(81)90480-4
更新日期：1981-03-15 00:00:00
abstract：:Analyses of cytoplasmic estrogen receptor and progesterone receptor were performed on tumor specimens from 32 patients with uterine sarcomas. While the median and maximal quantitative levels of binding were lower than those observed in endometrial carcinomas, examples of estrogen receptor- and progesterone receptor-po...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(84)80135-0
更新日期：1984-10-15 00:00:00
abstract：OBJECTIVE:The objective of the study was to evaluate the differences in vaginal culture, microscopy, and Gram stain between postmenopausal women who wear pessaries and those who do not to explain pessary-related, bothersome vaginal discharge. STUDY DESIGN:Postmenopausal women not using exogenous estrogen who had eithe...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2014.07.024
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to evaluate the feasibility and efficacy of laparoscopic radiofrequency ablation of uterine fibroids. STUDY DESIGN:Eighteen women with symptomatic intramural uterine myomas underwent radiofrequency ablation under laparoscopic guidance. Postoperative sonographic evaluations of th...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2004.10.591
更新日期：2005-03-01 00:00:00
abstract：:The specific purposes of this report are twofold: (1) to summarize the descriptive epidemiology of urethral-vesical dysfunction in a population of 1439 female patients referred for urodynamic evaluation of symptoms and (2) to analyze the association between demographic, clinical, and investigative data. On the basis o...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(85)90016-x
更新日期：1985-01-15 00:00:00
abstract：:The treatment of two pregnant thyrotoxic patients with propranolol alone is discussed. One patient had a spontaneous remission during pregnancy wile taking propranolol. In the other patient treatment with propranolol allowed postponement of definitive therapy until after delivery, when the fetus was no longer at risk....
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(75)90648-1
更新日期：1975-01-15 00:00:00
