Cold storage of rat skeletal muscle free flaps and pre-ischemic perfusion with modified UW solution.

Abstract:

:We used the rat medial gastrocnemius free flap, based on a pedicle of the femoral artery and vein, in order to test the tolerance of skeletal muscle to cold ischemia-reperfusion (IR) injury, and to determine whether tolerance can be enhanced by pre-ischemic perfusion with tissue/organ preservation solutions. Muscle flaps (n = 6 per group) were subjected to variable periods of cold storage (0, 1, 2, 3, or 4 days) and 24-h normothermic reperfusion. Muscle viability, as determined by nitroblue tetrazolium (NBT) histochemical staining of viable mitochondria and supported by histological examination, was 100%, 26%, 11%, 4%, and 1%, respectively. Using 24-h cold storage/24-h reperfusion as the experimental conditions, groups of muscle flaps (n = 5 per group) were perfused immediately before cold storage with either modified, colloid-free University of Wisconsin (UW) solution, a solution described by Kohout et al. (Br J Plast Surg 1995;48:132-144) or normal saline. Perfusion with modified UW solution or Kohout's solution increased survival to 33% (P < 0.05) and 28% (not statistically significant), respectively, compared with the 19% viability of separate groups of nonperfused or saline-perfused controls. These findings indicate that cold-stored skeletal muscle is highly susceptible to cold IR injury and that the viability can be increased by prior perfusion with a tissue preservation solution such as modified UW solution.

journal_name

Microsurgery

journal_title

Microsurgery

authors

Wagh M,Pantazi G,Romeo R,Hurley JV,Morrison WA,Knight KR

doi

10.1002/1098-2752(2000)20:7<343::aid-micr6>3.0.co;

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

343-9

issue

7

eissn

0738-1085

issn

1098-2752

pii

10.1002/1098-2752(2000)20:7<343::AID-MICR6>3.0.CO;

journal_volume

20

pub_type

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