Abstract:
:The cytologic localization and cellular levels of insulin-like growth factor binding protein-4 (IGFBP-4) in follicular and stromal compartments of normal and polycystic ovary syndrome (PCOS) ovaries during follicular growth and regression were investigated by the avidin/biotin immunoperoxidase method with a polyclonal antibody to human IGFBP-4, and a comparative assessment of IGFBP-4 expression in normal and PCOS ovaries was provided. In normal human ovaries, IGFBP-4 was immunolocalized to the oocyte throughout follicular growth, while the surrounding granulosa and theca cells were negligible for IGFBP-4 immunostaining in primordial, preantral and antral follicles. IGFBP-4 immunostaining became apparent, however, in the lutein cells of corpora lutea and the granulosa and theca cells of atretic follicles. In PCOS ovaries, prominent immunostaining for IGFBP-4 was apparent not only in the oocyte, but also in the surrounding granulosa cells in preantral follicles. In antral follicles from PCOS women without hyperinsulinemia, IGFBP-4 immunostaining was more prominent in the granulosa cells than the theca cells, whereas in antral follicles from PCOS women with hyperinsulinemia IGFBP-4 immunostaining was more prominent in the theca cells than the granulosa cells. Furthermore, in atretic follicles within PCOS ovaries IGFBP-4 immunostaining was prominent in the theca cells, regardless of the association of hyperinsulinemia. These results demonstrate for the first time that there is a great difference in cellular expression of IGFBP-4 between normal and PCOS human ovaries. In light of the high affinity of IGFBP-4 for IGF-1, the abundant expression of IGFBP-4 in granulosa and theca cells of preantral and antral follicles of PCOS ovaries may lead to decreases in the bioavailability of IGF-I in those follicles. The decrease in IGF-I-mediated stimulation of gonadotropin actions on granulosa and theca cells in preantral and antral follicles may impair the induction of aromatase activity, causing an androgenic microenvironment which is characteristic of atretic follicles and PCOS follicles.
journal_name
Endocr Jjournal_title
Endocrine journalauthors
Peng X,Maruo T,Samoto T,Mochizuki Mdoi
10.1507/endocrj.43.269subject
Has Abstractpub_date
1996-06-01 00:00:00pages
269-78issue
3eissn
0918-8959issn
1348-4540journal_volume
43pub_type
杂志文章abstract::Thyrotropin-releasing hormone (TRH)-stimulated thyroid stimulating hormone (TSH) response in normal subjects is suppressed by oral glucose administration. Pharmacologic studies indicate that this suppressive action of glucose is mediated by an increase in hypothalamic somatostatin (SRIH) tone. Since activation of the ...
journal_title:Endocrine journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1507/endocrj.52.69
更新日期:2005-02-01 00:00:00
abstract::KRN568 is a calcimimetic compound which acts on the calcium sensing receptors (CaR) on the parathyroid gland to suppress secretion of PTH. A recent report has demonstrated that CaRs are expressed on cultured human antral gastrin cells and that gastrin secretion is stimulated by an increase in extracellular calcium lev...
journal_title:Endocrine journal
pub_type: 临床试验,杂志文章
doi:10.1507/endocrj.47.517
更新日期:2000-10-01 00:00:00
abstract::To examine the specific changes in gene expression in thyroid carcinomas, we performed sequence specific-differential display (SS-DD) analysis by using a specific degenerate primer for the superfamily of the small G protein. After subcloning and sequencing analysis, one of the genes that showed decreased expression in...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.46.459
更新日期:1999-06-01 00:00:00
abstract::We previously developed a radioreceptor assay which is presumably specific for detection of blocking-type anti-TSH receptor (TSHR) antibodies using unsolubilized porcine TSHR. Employing this assay, we measured blocking TSH-binding inhibitory immunoglobulin (TBII) in the sera from 30 untreated Graves' patients and comp...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.48.703
更新日期:2001-12-01 00:00:00
abstract::Adult growth hormone deficiency (GHD) is a well defined clinical condition, which is characterized by abnormal body composition, impaired physical activity and decreased quality of life. In addition, in recent years, growing interest has been shown towards cardiovascular risks in adult patients affected by GHD. In thi...
journal_title:Endocrine journal
pub_type: 杂志文章,评审
doi:10.1507/endocrj.EJ15-0337
更新日期:2015-01-01 00:00:00
abstract::To evaluate the prevalence of lymphocytic infiltrate in a large series of pituitary adenomas, we retrospectively studied tumor tissues from 1400 patients. Based on immunocytochemical data, tumors were classified as PRL (n=411), multihormonal (n=310), immunonegative (n=275), ACTH (n=166), GH (n=137), alpha subunit (n=4...
journal_title:Endocrine journal
pub_type: 杂志文章,多中心研究
doi:10.1507/endocrj.45.357
更新日期:1998-06-01 00:00:00
abstract::In the last ten years a liquid formulation of liothyronine (L-T3) became available. To date, no studies on its systematic use have been reported. This study is aimed at assessing the reliability of liquid L-T3 in achieving target TSH in patients with differentiated thyroid cancers (DTC). Twenty-one high risk DTC patie...
journal_title:Endocrine journal
pub_type: 临床试验,杂志文章
doi:10.1507/endocrj.EJ16-0040
更新日期:2016-06-30 00:00:00
abstract::Gonadotropin-releasing hormone (GnRH) and its agonists have been known to directly affect steroid hormone production in human granulosa cells. In this study, we examined effects of GnRH on Ca2+ mobilization, protein kinase C activity and steroidogenesis of human granulosa cells. Human granulosa cells were harvested by...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.45.175
更新日期:1998-04-01 00:00:00
abstract::Sarcopenia is an emerging risk factor for metabolic disorders. No study of the association of sarcopenia with insulin resistance, diabetes, and metabolic syndrome (MS) according to age group and obesity status in the general population has been reported. We investigated these associations in the Korean population. Par...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej13-0244
更新日期:2014-01-01 00:00:00
abstract::We report on two siblings with classic simple virilizing 21-hydroxylase deficiency whose neonatal screening for serum 17alpha-hydroxyprogesterone (17-OHP) gave normal results. The proband, a girl with clitoromegaly whose screening 17-OHP value had been 9.2 ng/ml, was diagnosed at the age of 6 months, and her elder bro...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.45.427
更新日期:1998-06-01 00:00:00
abstract::The human endometrium undergoes cyclical changes including proliferation, differentiation, tissue breakdown, and shedding (menstruation) throughout a woman's reproductive life. The postovulatory rise in ovarian progesterone induces profound remodeling and differentiation of the estradiol-primed endometrium. This chang...
journal_title:Endocrine journal
pub_type: 杂志文章,评审
doi:10.1507/endocrj.k08e-067
更新日期:2008-10-01 00:00:00
abstract::Betacellulin (BTC) purified from mouse beta cell tumor (betaTC-3) is a new member of the epidermal growth factor (EGF) family which can bind receptor tyrosine kinase, EGF receptor (erbB1) and erbB4. It has been demonstrated that proBTC mRNA was abundantly expressed in human pancreas tissue, and that BTC converted amyl...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.46.755
更新日期:1999-12-01 00:00:00
abstract::Thyroid hormone plays an important role in growth hormone (GH) synthesis and secretion. To study the relationship between thyroid function and urinary GH secretion in the hyperthyroid and hypothyroid states, we measured thyroid hormones, simultaneously with serum and urinary GH levels, in 54 patients with thyroid dise...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.41.517
更新日期:1994-10-01 00:00:00
abstract::The identification of unique point mutations in patients with pseudohypoparathyroidism (PHP) with Albright's hereditary osteodystrophy (AHO) in different ethnic backgrounds has proved that defects within the Gs alpha gene account for Gs alpha deficiency in those patients. To search a mutation hot spot of the Gs alpha ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.44.621
更新日期:1997-08-01 00:00:00
abstract::The aim of this study was to assess the changes in insulin secretion and insulin sensitivity in relation to fasting and 2-hour plasma glucose (PG) levels and to assess the independent contributions of their impairments to non-diabetic hyperglycemia. A total of 2157 Japanese workers (mean age 52.6±7.3 years and mean B...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej11-0157
更新日期:2012-01-01 00:00:00
abstract::21-hydroxylase deficiency (21-OHD) is the most common type of congenital adrenal hyperplasia. In addition to the clinical problems caused by adrenal insufficiency and androgen excess, a risk for obesity and metabolic syndrome during young adulthood is a major ramification of the disease. Although glucocorticoid therap...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ16-0194
更新日期:2016-11-30 00:00:00
abstract::Guanylin (Gn), a bioactive peptide, and its receptor, guanylyl cyclase-C (GC-C), are primarily present in the intestine and maintain homeostasis in body fluids. Recently, rats whose macrophages overexpress Gn and GC-C were found to be resistant to diet-induced obesity. Considering that obesity is strongly related to a...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ15-0193
更新日期:2015-01-01 00:00:00
abstract::To assess the total daily inulin dose (TDD) and contribution of basal insulin to TDD and to identify the predictive factors for insulin requirement profiles in subjects with type 2 diabetes, we retrospectively examined insulin requirement profiles of 275 hospitalized subjects treated with basal-bolus insulin therapy (...
journal_title:Endocrine journal
pub_type: 杂志文章,多中心研究
doi:10.1507/endocrj.EJ14-0487
更新日期:2015-01-01 00:00:00
abstract::Following the accident at the Fukushima Daiichi Nuclear Power Station which occurred on March 11, 2011 due to the Eastern Japan Great Earthquake (the Accident), there have been concerns over elevation of the risk of thyroid cancer among children due to internal exposure to radioactive iodine. In Fukushima Prefecture, ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej14-0032
更新日期:2014-01-01 00:00:00
abstract::This is a case report of an 18-year-old man with central diabetes incipidus (DI). An MRI done three months after the onset of the DI did not disclose a responsible lesion. Four months later, a second MRI showed the location of the tumor origin at the upper pituitary stalk and median eminence. Eight months later, the t...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.46.263
更新日期:1999-04-01 00:00:00
abstract::It has been demonstrated the involvement of branched-chain amino acids (BCAA) on obesity and related metabolic disorder. We investigated the effects of branched-chain amino acids (BCAA) on obesity and on glucose/fat homeostasis in mice fed on a high-fat (45%) diet. BCAA was dissolved in 0.5% methylcellulose and added ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k10e-221
更新日期:2011-01-01 00:00:00
abstract::It is known that endoplasmic reticulum (ER) stress is provoked under diabetic conditions and is possibly involved in the development of insulin resistance. In this study, using ER stress-activated indicator (ERAI) transgenic mice which express green fluorescent protein under ER stress conditions, we directly evaluated...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k09e-140
更新日期:2009-01-01 00:00:00
abstract::Thyroid disease is known to be associated with pulmonary arterial hypertension (PAH). We investigated the prevalence of thyroid disease in patients with idiopathic PAH (IPAH) or heritable PAH (HPAH), and the factors affecting the pathogenesis of thyroid disease. We retrospectively evaluated 59 patients with IPAH or HP...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.EJ17-0155
更新日期:2017-12-28 00:00:00
abstract::We recently reported three cases of severe hypertriglyceridemia caused by tamoxifen-treatment after breast cancer surgery. In that report we showed that one of the three patients had apo E3/3 and another had E4/2 phenotype. There are few data about the relationship between apoprotein E phenotype, one of modifiers of l...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.45.255
更新日期:1998-04-01 00:00:00
abstract::We describe a case of diabetes insipidus (DI) due to a pituitary tumor in a 33-year-old pregnant woman who developed a sudden onset of polyuria (over 8 l/day) and polydipsia at 30 weeks of gestation. Her plasma concentration of vasopressin (AVP) was low compared with high serum osmolality (298 mOsm/kg), and her urine ...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.43.577
更新日期:1996-10-01 00:00:00
abstract::Rickets and osteomalacia are diseases characterized by impaired mineralization of bone matrix. Recent investigations revealed that the causes for rickets and osteomalacia are quite variable. While these diseases can severely impair the quality of life of the affected patients, rickets and osteomalacia can be completel...
journal_title:Endocrine journal
pub_type: 杂志文章,实务指引
doi:10.1507/endocrj.EJ15-0289
更新日期:2015-01-01 00:00:00
abstract::Obesity consists of hypertrophy and hyperplasia of adipocytes. Although the number of adipocytes is influenced by anatomical location, nutritional environment, hormone and genetic variation, it has been thought to be determined by the proliferation of precursor cells and subsequent differentiation. However, our recent...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.ej13-0053
更新日期:2013-01-01 00:00:00
abstract::Exercise is a fundamental component of diabetes management. However, choosing inappropriate type or timing of exercise is associated with mild or severe hypoglycemia either during exercise or several hours after exercise. Several studies have shown that impaired counterregulatory responses triggers hypoglycemia. There...
journal_title:Endocrine journal
pub_type: 杂志文章,评审
doi:10.1507/endocrj.EJ19-0153
更新日期:2019-09-28 00:00:00
abstract::The measurement of TSH receptor antibody (TRAb) has been recommended to predict the risk of neonatal hyperthyroidism (NH) in pregnant women with Graves' disease (GD). For the first generation TRAb (TRAb1) assay with commercial kit (Brahms, Berlin, Germany; or Cosmic co., Tokyo, Japan) an arbitrary limit of 40 U/l or 5...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.k06-196
更新日期:2007-08-01 00:00:00
abstract::We here describe two patients with empty sella syndrome who had variable and abnormal ACTH and cortisol secretory profiles. The patients are a 58-year old male and a 41-year old female, both of whom were neurotic. In both cases, low-dose dexamethasone suppression tests (1 mg, p.o., overnight) caused variable responses...
journal_title:Endocrine journal
pub_type: 杂志文章
doi:10.1507/endocrj.43.417
更新日期:1996-08-01 00:00:00