Abstract：
:Cultures were initiated in Madin-Darby bovine kidney (MDBK) cells from ME49 strain bradyzoites. Specific antibody staining showed that two populations of parasites exist, one being a predominant population of tachyzoites that were positive for the tachyzoite-specific marker SAG1 and negative for the bradyzoite-specific marker P36. All of these parasites expressed the dense granule molecule GRA5, which in larger clusters was seen faintly in the membrane of the parasitophorous vacuole. No rosette formation or monolayer destruction was observed. Also seen was a sub-population of bradyzoites that were positive for P36 and negative for SAG1. Approximately 90% of these parasites expressed the matrix molecule P29. These parasites were also positive for the dense granule molecule GRA5, which was highly concentrated in the wall of the cyst. These bradyzoite clusters contained fewer parasites and were smaller in diameter than those expressing tachyzoite markers.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Lane A,Soete M,Dubremetz JF,Smith JEdoi
10.1007/s004360050123subject
Has Abstractpub_date
1996-01-01 00:00:00pages
340-6issue
4eissn
0932-0113issn
1432-1955journal_volume
82pub_type
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