Differential expression of methicillin resistance by different biofilm-negative Staphylococcus epidermidis transposon mutant classes.


:Biofilm formation mediated by polysaccharide intercellular adhesin (PIA) is the major virulence factor of Staphylococcus epidermidis and is often associated with methicillin resistance. Transposon Tn917 insertions leading to a biofilm-negative phenotype in the biofilm-producing S. epidermidis strain 1457 (mecA-negative) were transferred into the methicillin-resistant, biofilm-producing S. epidermidis 1057 (mecA-positive) by transduction. According to their phenotypes and genotypes, the mutants could be separated into genetic classes I to IV (D. Mack, H. Rohde, S. Dobinsky, J. Riedewald, M. Nedelmann, J. K. M. Knobloch, H.-A. Elsner, and H. H. Feucht, Infect. Immun. 68:3799-3807, 2000). All transductants of S. epidermidis 1057 had phenotypes for biofilm formation similar to those of the corresponding mutants of S. epidermidis 1457. With a mecA-specific probe, identical hybridization patterns were observed for wild-type S. epidermidis 1057 and all the transductants. There were minor changes in oxacillin MICs for Class II and III transductants compared to those for wild-type S. epidermidis 1057. On population analysis, S. epidermidis 1057 displayed a heterogeneous expression type of resistance with an oxacillin MIC of > or =6 microg/ml for more than 90% of the cells. An almost identical profile was observed with biofilm-negative class I mutants, where the transposon insertions inactivate the icaADBC gene locus essential for PIA synthesis. In contrast, class III mutants were more sensitive to oxacillin with a MIC of < or =1 microg/ml for more than 90% of the cells. The class IV mutant displayed homogeneous resistance with a MIC of > or =50 microg/ml for more than 90% of the cells. On oxacillin gradient plates, the class II mutant displayed decreased resistance. Apparently, different independent mutations leading to a biofilm-negative phenotype of S. epidermidis by influencing expression of icaADBC on the level of transcription significantly influence the expression of methicillin resistance. However, transcription of mecA was not significantly altered in the different transductants compared to the wild type, independent of mecA induction with oxacillin, indicating that other mechanisms influencing phenotypic expression of methicillin resistance are involved.


Mack D,Sabottke A,Dobinsky S,Rohde H,Horstkotte MA,Knobloch JK




Has Abstract


2002-01-01 00:00:00












  • Penetration of colistin into cerebrospinal fluid.

    abstract::Colistin penetration into the cerebrospinal fluid (CSF) was studied in five critically ill adult patients receiving colistin methanesulfonate for infections by multiresistant gram-negative bacilli. Colistin concentrations were determined in paired serum and CSF samples, with the latter taken by lumbar puncture, with t...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Markantonis SL,Markou N,Fousteri M,Sakellaridis N,Karatzas S,Alamanos I,Dimopoulou E,Baltopoulos G

    更新日期:2009-11-01 00:00:00

  • Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolates of Enterobacter cloacae in a Taiwanese hospital.

    abstract::The prevalence of three plasmid-mediated quinolone resistance determinants, QnrA, QnrB, and QnrS, among 526 nonreplicate clinical isolates of Enterobacter cloacae collected at a Taiwanese university hospital in 2004 was determined by PCR and colony hybridization, and the association of Qnr with the IMP-8 metallo-beta-...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Wu JJ,Ko WC,Tsai SH,Yan JJ

    更新日期:2007-04-01 00:00:00

  • Study of use of cefdinir versus cephalexin for treatment of skin infections in pediatric patients. The Cefdinir Pediatric Skin Infection Study Group.

    abstract::Three hundred ninety-four patients, aged 6 months to 12 years, entered a multicenter, randomized, controlled, investigator-blind study comparing cefdinir, 7 mg/kg of body weight twice a day, with cephalexin, 10 mg/kg four times a day, each given for 10 days. The most common infections treated were impetigo and seconda...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Tack KJ,Keyserling CH,McCarty J,Hedrick JA

    更新日期:1997-04-01 00:00:00

  • Multicenter Study of the Relationship between Carbapenem MIC Values and Clinical Outcome of Patients with Acinetobacter Bacteremia.

    abstract::The Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) offer different recommendations for carbapenem MIC susceptibility breakpoints for Acinetobacter species. In addition, the clinical efficacy of the intermediate category remains uncertain. ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章,多中心研究


    authors: Yang YS,Wang YC,Kuo SC,Chen CT,Liu CP,Liu YM,Chen TL,Lee YT

    更新日期:2017-08-24 00:00:00

  • The novel parainfluenza virus hemagglutinin-neuraminidase inhibitor BCX 2798 prevents lethal synergism between a paramyxovirus and Streptococcus pneumoniae.

    abstract::An association exists between respiratory viruses and bacterial infections. Prevention or treatment of the preceding viral infection is a logical goal for reducing this important cause of morbidity and mortality. The ability of the novel, selective parainfluenza virus hemagglutinin-neuraminidase inhibitor BCX 2798 to ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Alymova IV,Portner A,Takimoto T,Boyd KL,Babu YS,McCullers JA

    更新日期:2005-01-01 00:00:00

  • In vitro activities of five fluoroquinolone compounds against strains of Streptococcus pneumoniae with resistance to other antimicrobial agents.

    abstract::Ciprofloxacin, clinafloxacin, PD 131628, sparfloxacin, and trovafloxacin were tested against 236 strains of Streptococcus pneumoniae, most of which were resistant to other agents. Resistance to multiple antibiotics did not affect the organism's susceptibility to the fluoroquinolones. The fluoroquinolones with in vitro...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Barry AL,Fuchs PC,Brown SD

    更新日期:1996-10-01 00:00:00

  • Roles of beta-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniae.

    abstract::Two clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae were noted to be less susceptible than expected to imipenem. Both were missing outer membrane proteins that serve as channels for antibiotic entry. The role of beta-lactamase in resistance was investigated by eliminating t...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Martínez-Martínez L,Pascual A,Hernández-Allés S,Alvarez-Díaz D,Suárez AI,Tran J,Benedí VJ,Jacoby GA

    更新日期:1999-07-01 00:00:00

  • Attenuation of colistin bactericidal activity by high inoculum of Pseudomonas aeruginosa characterized by a new mechanism-based population pharmacodynamic model.

    abstract::Colistin is increasingly being utilized against Gram-negative pathogens, including Pseudomonas aeruginosa, resistant to all other antibiotics. Since limited data exist regarding killing by colistin at different initial inocula (CFUo), we evaluated killing of Pseudomonas aeruginosa by colistin at several CFUo and devel...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Bulitta JB,Yang JC,Yohonn L,Ly NS,Brown SV,D'Hondt RE,Jusko WJ,Forrest A,Tsuji BT

    更新日期:2010-05-01 00:00:00

  • Comparative efficacy of cephalexin and ampicillin for shigellosis and other types of acute diarrhea in infants and children.

    abstract::Most ampicillin-resistant Shigella are susceptible to cephalexin. Randomized treatment with cephalexin or ampicillin was given to 154 infants and children with acute diarrhea. Rectal swab cultures revealed Shigella in 42%, Salmonella in 6%, enteropathogenic Escherichia coli in 2%, and no pathogen in 50%. Cephalexin fa...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Nelson JD,Haltalin KC

    更新日期:1975-04-01 00:00:00

  • Biochemical properties of beta-lactamase produced by Legionella gormanii.

    abstract::beta-Lactamase was purified from a strain of Legionella gormanii. The molecular weight of the purified enzyme was 25,000, and its isoelectric point was 10.5. The enzyme hydrolyzed oxyiminocephalosporins, cephamycins, penicillins, and imipenem. The enzyme activity was inhibited by EDTA, Hg2+, and Cu2+, but not by clavu...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Fujii T,Sato K,Miyata K,Inoue M,Mitsuhashi S

    更新日期:1986-05-01 00:00:00

  • Impact of antifungal prophylaxis on colonization and azole susceptibility of Candida species.

    abstract::Two large studies compared posaconazole and fluconazole or itraconazole for prophylaxis in subjects undergoing allogeneic hematopoietic stem cell transplantation or subjects with acute myelogenous leukemia. To assess the impact of prophylaxis on colonization and the development of resistance in Saccharomyces yeasts, i...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章,随机对照试验


    authors: Mann PA,McNicholas PM,Chau AS,Patel R,Mendrick C,Ullmann AJ,Cornely OA,Patino H,Black TA

    更新日期:2009-12-01 00:00:00

  • Susceptibility to thrombin-induced platelet microbicidal protein is associated with increased fluconazole efficacy against experimental endocarditis due to Candida albicans.

    abstract::Platelet microbicidal proteins (PMPs) are believed to be integral to host defense against endovascular infection. We previously demonstrated that susceptibility to thrombin-induced PMP 1 (tPMP-1) in vitro negatively influences Candida albicans virulence in the rabbit model of infective endocarditis (IE). This study ev...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Yeaman MR,Cheng D,Desai B,Kupferwasser LI,Xiong YQ,Gank KD,Edwards JE Jr,Bayer AS

    更新日期:2004-08-01 00:00:00

  • In vitro evaluation of the effectiveness of the macrolide rokitamycin and chlorpromazine against Acanthamoeba castellanii.

    abstract::The present study demonstrates the in vitro effectiveness of the macrolide rokitamycin and the phenothiazine compound chlorpromazine against Acanthamoeba castellanii. Growth curve evaluations revealed that both drugs inhibit trophozoite growth in dose- and time-dependent ways. The effects of both drugs when they were ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Mattana A,Biancu G,Alberti L,Accardo A,Delogu G,Fiori PL,Cappuccinelli P

    更新日期:2004-12-01 00:00:00

  • Examination of major disagreements in susceptibility test results by Autobac-1 and MS-2.

    abstract::Major disagreements between two automated susceptibility test instruments, Autobac-1 and MS-2, generated from 4,213 tests on 630 bacterial isolates, were analyzed. The major disagreement rate between the instruments was only 7.4%. The highest major disagreement rates for bacteria were with Staphylococcus epidermidis, ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Lindsey NJ,Barnes WG

    更新日期:1981-07-01 00:00:00

  • Penicillin-binding proteins in a Staphylococcus aureus strain resistant to specific beta-lactam antibiotics.

    abstract::The penicillin-binding proteins (PBPs) of a clinical isolate of Staphylococcus aureus specifically resistant to oral cephalosporins were compared with those of a susceptible strain. In the resistant strain, PBP3 (75,000 molecular weight) was missing or had substantially (greater than 100-fold) reduced affinity for pen...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Georgopapadakou NH,Smith SA,Bonner DP

    更新日期:1982-07-01 00:00:00

  • Activity of daptomycin against Listeria monocytogenes isolates from cerebrospinal fluid.

    abstract::We tested the activity of daptomycin against 76 Listeria monocytogenes isolates from cerebrospinal fluid by broth dilution and Etest methods. For the broth dilution method, the MIC range was 1.0 to 8.0 and the MIC at which 90% of the isolates tested were inhibited (MIC(90)) was 4.0 mg/liter. For the Etest method, the ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Spanjaard L,Vandenbroucke-Grauls CM

    更新日期:2008-05-01 00:00:00

  • N-acylated derivatives of sulfamethoxazole and sulfafurazole inhibit intracellular growth of Chlamydia trachomatis.

    abstract::Antibacterial compounds with novel modes of action are needed for management of bacterial infections. Here we describe a high-content screen of 9,800 compounds identifying acylated sulfonamides as novel growth inhibitors of the sexually transmitted pathogen Chlamydia trachomatis. The effect was bactericidal and distin...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Marwaha S,Uvell H,Salin O,Lindgren AE,Silver J,Elofsson M,Gylfe A

    更新日期:2014-05-01 00:00:00

  • Selective interaction of the human immunodeficiency virus type 1 reverse transcriptase nonnucleoside inhibitor efavirenz and its thio-substituted analog with different enzyme-substrate complexes.

    abstract::Accumulating data have brought the nonnucleoside reverse transcriptase (RT) inhibitors (NNRTIs) into the forefront of antiretroviral therapy. Among the emerging compounds in this class, a particularly attractive one is efavirenz (Sustiva), recently approved for clinical use by the U.S. Food and Drug Administration. In...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Maga G,Ubiali D,Salvetti R,Pregnolato M,Spadari S

    更新日期:2000-05-01 00:00:00

  • Novel Protease Inhibitors Containing C-5-Modified bis-Tetrahydrofuranylurethane and Aminobenzothiazole as P2 and P2' Ligands That Exert Potent Antiviral Activity against Highly Multidrug-Resistant HIV-1 with a High Genetic Barrier against the Emergence of

    abstract::Combination antiretroviral therapy has achieved dramatic reductions in the mortality and morbidity in people with HIV-1 infection. Darunavir (DRV) represents a most efficacious and well-tolerated protease inhibitor (PI) with a high genetic barrier to the emergence of drug-resistant HIV-1. However, highly DRV-resistant...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Takamatsu Y,Aoki M,Bulut H,Das D,Amano M,Sheri VR,Kovari LC,Hayashi H,Delino NS,Ghosh AK,Mitsuya H

    更新日期:2019-07-25 00:00:00

  • Activities of benzimidazole D- and L-ribonucleosides in animal models of cytomegalovirus infections.

    abstract::Since human cytomegalovirus (HCMV) does not infect or replicate in nonhuman cells and tissues, there are few animal models currently available for evaluation of antiviral therapies for these infections. In the present studies, we utilized two different models in which severe combined immunodeficient (SCID) mice were i...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Kern ER,Hartline CB,Rybak RJ,Drach JC,Townsend LB,Biron KK,Bidanset DJ

    更新日期:2004-05-01 00:00:00

  • In vitro antimicrobial activity of ceftizoxime against glucose-nonfermentative gram-negative rods.

    abstract::Ceftizoxime, a new cephalosporin, was active against Pseudomonas cepacia, Flavobacterium meningosepticum, Alcaligenes faecalis, and Acinetobacter calcoaceticus and was more potent against Pseudomonas aeruginosa and Pseudomonas putida than was carbenicillin. ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Yabuuchi E,Ito T,Tanimura E,Yamamoto N,Ohyama A

    更新日期:1981-07-01 00:00:00

  • Emergence of reduced susceptibility and resistance to fluoroquinolones in Escherichia coli in Taiwan and contributions of distinct selective pressures.

    abstract::A survey of 1,203 Escherichia coli isolates from 44 hospitals in Taiwan revealed that 136 (11.3%) isolates were resistant to fluoroquinolones and that another 261 (21.7%) isolates had reduced susceptibility. Resistance was more common in isolates responsible for hospital-acquired (mostly in intensive care units) infec...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: McDonald LC,Chen FJ,Lo HJ,Yin HC,Lu PL,Huang CH,Chen P,Lauderdale TL,Ho M

    更新日期:2001-11-01 00:00:00

  • HIV-positive nigerian adults harbor significantly higher serum lumefantrine levels than HIV-negative individuals seven days after treatment for Plasmodium falciparum infection.

    abstract::Management of coinfection with malaria and HIV is a major challenge to public health in developing countries, and yet potential drug-drug interactions between antimalarial and antiviral regimens have not been adequately investigated in people with both infections. Each of the constituent components of artemether-lumef...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Chijioke-Nwauche I,van Wyk A,Nwauche C,Beshir KB,Kaur H,Sutherland CJ

    更新日期:2013-09-01 00:00:00

  • Surveillance of carbapenem-resistant Klebsiella pneumoniae: tracking molecular epidemiology and outcomes through a regional network.

    abstract::Carbapenem resistance in Gram-negative bacteria is on the rise in the United States. A regional network was established to study microbiological and genetic determinants of clinical outcomes in hospitalized patients with carbapenem-resistant (CR) Klebsiella pneumoniae in a prospective, multicenter, observational study...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: van Duin D,Perez F,Rudin SD,Cober E,Hanrahan J,Ziegler J,Webber R,Fox J,Mason P,Richter SS,Cline M,Hall GS,Kaye KS,Jacobs MR,Kalayjian RC,Salata RA,Segre JA,Conlan S,Evans S,Fowler VG Jr,Bonomo RA

    更新日期:2014-07-01 00:00:00

  • In vitro susceptibilities of Candida bloodstream isolates to the new triazole antifungal agents BMS-207147, Sch 56592, and voriconazole.

    abstract::BMS-207147, Sch 56592, and voriconazole are three new investigational triazoles with broad-spectrum antifungal activity. The in vitro activities of these three agents were compared with those of itraconazole and fluconazole against 1,300 bloodstream isolates of Candida species obtained from over 50 different medical c...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Pfaller MA,Messer SA,Hollis RJ,Jones RN,Doern GV,Brandt ME,Hajjeh RA

    更新日期:1998-12-01 00:00:00

  • Chronic Salmonella bacteriuria with intermittent bacteremia treated with low doses of amoxicillin or ampicillin.

    abstract::Amoxicillin and ampicillin were compared at a dose of 250 mg twice daily for 4 weeks to treat Salmonella typhi or Salmonella paratyphi A chronic bacteriuria with intermittent bacteremia. Eleven patients received amoxicillin, and 15 received ampicillin. Concentrations of the two drugs were measured in the urine and ser...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Bassily SB,Kilpatrick ME,Farid Z,Mikhail IA,El-Masry NA

    更新日期:1981-11-01 00:00:00

  • Influence of P-glycoprotein inhibitors on accumulation of macrolides in J774 murine macrophages.

    abstract::The influence of inhibitors of P-glycoprotein (verapamil [VE], cyclosporine [CY], and GF120918 [GF]) on the cell handling of macrolides (erythromycin [ERY], clarithromycin [CLR], roxithromycin [ROX], azithromycin [AZM], and telithromycin [TEL]) was examined in J774 murine macrophages. The net influx rates of AZM and T...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Seral C,Michot JM,Chanteux H,Mingeot-Leclercq MP,Tulkens PM,Van Bambeke F

    更新日期:2003-03-01 00:00:00

  • Population pharmacokinetics and pharmacodynamics of efavirenz, nelfinavir, and indinavir: Adult AIDS Clinical Trial Group Study 398.

    abstract::The present population pharmacokinetic (PK) and pharmacodynamic (PD) study modeled the effects of covariates including drug adherence and the coadministration of protease inhibitors (PIs) on the pharmacokinetics of efavirenz (EFV) and the relationship between EFV exposure and virological failure in patients who failed...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Pfister M,Labbé L,Hammer SM,Mellors J,Bennett KK,Rosenkranz S,Sheiner LB,Adult AIDS Clinical Trial Group Study 398.

    更新日期:2003-01-01 00:00:00

  • A quinazoline-2,4-diamino analog suppresses Vibrio cholerae flagellar motility by interacting with motor protein PomB and induces envelope stress.

    abstract::Vibrio cholerae strains of serogroups O1 and O139, the causative agents of the diarrheal illness cholera, express a single polar flagellum powered by sodium motive force and require motility to colonize and spread along the small intestine. In a previous study, we described a high-throughput assay for screening for sm...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Wang H,Zhang L,Silva AJ,Benitez JA

    更新日期:2013-08-01 00:00:00

  • Plasmid-determined resistance to boron and chromium compounds in Pseudomonas aeruginosa.

    abstract::Plasmids determining resistance to arsenic, mercury, silver, and tellurium compounds in Escherichia coli and Pseudomonas aeruginosa were tested for resistance to 40 other metal compounds. Resistance to trivalent boron and hexavalent chromium compounds was a property of certain P. aeruginosa plasmids. ...

    journal_title:Antimicrobial agents and chemotherapy

    pub_type: 杂志文章


    authors: Summers AO,Jacoby GA

    更新日期:1978-04-01 00:00:00