Abstract:
:We report on the cloning of the gene smeT, which encodes the transcriptional regulator of the Stenotrophomonas maltophilia efflux pump SmeDEF. SmeT belongs to the TetR and AcrR family of transcriptional regulators. The smeT gene is located upstream from the structural operon of the pump genes smeDEF and is divergently transcribed from those genes. Experiments with S. maltophilia and the heterologous host Escherichia coli have demonstrated that SmeT is a transcriptional repressor. S1 nuclease mapping has demonstrated that expression of smeT is driven by a single promoter lying close to the 5' end of the gene and that expression of smeDEF is driven by an unique promoter that overlaps with promoter PSMET: The level of expression of smeT is higher in smeDEF-overproducing S. maltophilia strain D457R, which suggests that SmeT represses its own expression. Band-shifting assays have shown that wild-type strain S. maltophilia D457 contains a cellular factor(s) capable of binding to the intergenic smeT-smeD region. That cellular factor(s) was absent from smeDEF-overproducing S. maltophilia strain D457R. The sequence of smeT from D457R showed a point mutation that led to a Leu166Gln change within the SmeT protein. This change allowed overexpression of both smeDEF and smeT in D457R. It was noteworthy that expression of wild-type SmeT did not fully complement the smeT mutation in D457R. This suggests that the wild-type protein is not dominant over the mutant SmeT.
journal_name
Antimicrob Agents Chemotherjournal_title
Antimicrobial agents and chemotherapyauthors
Sánchez P,Alonso A,Martinez JLdoi
10.1128/aac.46.11.3386-3393.2002subject
Has Abstractpub_date
2002-11-01 00:00:00pages
3386-93issue
11eissn
0066-4804issn
1098-6596journal_volume
46pub_type
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journal_title:Antimicrobial agents and chemotherapy
pub_type: 杂志文章
doi:10.1128/AAC.00485-10
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journal_title:Antimicrobial agents and chemotherapy
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doi:10.1128/AAC.40.1.260
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doi:10.1128/aac.38.11.2572
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pub_type: 杂志文章
doi:10.1128/aac.47.5.1681-1688.2003
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pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章
doi:10.1128/AAC.42.7.1542
更新日期:1998-07-01 00:00:00
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journal_title:Antimicrobial agents and chemotherapy
pub_type: 杂志文章
doi:10.1128/aac.35.3.497
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doi:10.1128/AAC.43.1.85
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2015-09-01 00:00:00
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