Abstract：
:The aim of this project was to develop an analytical procedure with the required level of sensitivity for the determination of glucose concentrations in small volumes of unstimulated fasting whole saliva. The technique involves high-performance ion-exchange chromatography at high pH and pulsed amperometric detection. It has a high level of reproducibility, a sensitivity as low as 0.1 mumol/l and requires only 50 microliters samples (sensitivity = 0.002 pmol). Inhibition of glucose metabolism, by procedures such as collection into 0.1% (w/v) sodium fluoride, was shown to be essential if accurate results are to be obtained. Collection on to ice followed by storage at -20 degrees C was shown to be unsuitable and resulted in glucose loss by degradation. There were inter- and intraindividual variations in the glucose concentration in unstimulated mixed saliva (range; 0.02-0.4 mmol/l). The procedure can be used for the analysis of other salivary carbohydrates and for monitoring the clearance of dietary carbohydrates from the mouth.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Gough H,Luke GA,Beeley JA,Geddes DAdoi
10.1016/0003-9969(95)00121-2subject
Has Abstractpub_date
1996-02-01 00:00:00pages
141-5issue
2eissn
0003-9969issn
1879-1506pii
0003-9969(95)00121-2journal_volume
41pub_type
杂志文章abstract：:Tartrate-resistant acid phosphatase (TRAPase)-positive cells were examined during the early morphogenesis of mouse mandibular first molar teeth, using a simultaneous azo dye-coupling technique and confocal laser scanning microscopy (CLSM). CLSM of paraffin-embedded sections revealed that cells exhibiting TRAPase fluor...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(98)00019-3
更新日期：1998-05-01 00:00:00
abstract：:The peroxidase activity in human whole saliva is due to salivary peroxidase and, in some cases, myeloperoxidase; it is usually determined by spectrophotometric methods based on the rate of oxidation of chromogen substrates. Thiocyanate ion, a normal component of saliva, interferes with these kinetic assays by competin...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90095-6
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVE:To evaluatein vitro the antibacterial activity, the antibiofilm effect and the cytotoxic potential of mouthwashes containing Brazilian red propolis with or without fluoride. METHODS:The minimum inhibitory and bactericidal concentrations (MIC and MBC) against S. mutans, S. sanguinis, S. salivarius and L. case...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.104512
更新日期：2019-11-01 00:00:00
abstract：:The possible role of fibronectin in dental tissue repair was investigated by comparing its distribution and that of the 165-kDa fibronectin-binding membrane protein (165 kDa-FnBP) in odontoblasts underlying carious and sound dentine. By immunoperoxidase and light microscopy, fibronectin was localized in the dentine un...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00068-z
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to determine whether measurements of volatile sulfur compounds (VSCs) are useful to predict colonization of periodontopathic bacteria. For this purpose, we assessed the relationships among distributions of 4 species of periodontopathic bacteria in tongue coating and dental plaque, o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.09.019
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:Non-syndromic supernumerary teeth (NSST) are a common type of dental anomaly. microRNAs (miRNAs) play an important role in craniofacial and tooth development. Therefore, we hypothesized that single nucleotide polymorphisms (SNPs) of miRNAs may be associated with the susceptibility of NSST. MATERIALS AND METH...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104771
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:Several local factors can affect the wound-healing process, delaying its progression and postponing tissue homeostasis. It is known that local inflammation is related to wound healing; however, the maintenance of the inflammatory reaction can impair the proliferation and migration of oral mucosal cells. The a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.04.010
更新日期：2015-08-01 00:00:00
abstract：:The release studied by incubating tissue slices in an enriched, oxygenated medium in the presence and absence of secretagogues indicated that slices from 1-day-old rats released K+ in response to carbamylcholine, but not in response to epinephrine. The response to carbamylcholine was dose-related, with a 12.6 +/- 1.3 ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90100-5
更新日期：1982-01-01 00:00:00
abstract：:The scattering phase functions for HeNe-laser light of dentine sections 10-20 micron(s) thick were measured. The functions perpendicular to the tubules had first-order maxima at angles of 4 degrees for bovine dentine and 5 degrees for human dentine; those parallel to the tubules showed no first-order maxima. Several c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90098-f
更新日期：1991-01-01 00:00:00
abstract：:An outward flow of fluid through exposed dentine was demonstrated in anaesthetized cats. The flow was measured by observing the movement of the fat droplets of dilute milk in a glass capillary (i.d. 30 microns) with a microscope. The capillary was sealed to the dentine with a plastic cap. The resting flow rate through...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90087-o
更新日期：1992-03-01 00:00:00
abstract：OBJECTIVE:We previously proposed the hamster cheek pouch model of oral cancer for BNCT studies. We herein present the biodistribution of a non-toxic boron compound, GB-10 (Na(2)(10)B10H10), in this model to assess its potential for BNCT or BNCT enhanced Fast Neutron Therapy. MATERIALS AND METHODS:We evaluated the upta...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2003.10.003
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:Biomarkers in gingival crevicular fluid (GCF) have been investigated; however, measurements were limited by the small sample volume available. The aim of this study was to determine the levels of 40 different cytokines and chemokines in GCF samples. DESIGN:Eleven patients with generalised chronic periodontit...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.11.012
更新日期：2013-06-01 00:00:00
abstract：:Eight primary incisors obtained from a child with dentinogenesis imperfecta (DI) type II were examined histologically using light microscopy and scanning electron microscopy. In both the DI and control teeth, large dentinal canals were observed along the midline of the crown distributed mesial-distally and coursing to...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90116-5
更新日期：1985-01-01 00:00:00
abstract：:Various fruit juices with relatively low pH are known to have erosive effects on human tooth enamel in a reasonably short time. Honey, also with a relatively low pH, could do the same, but scanning electron microscopy showed no erosion of enamel by honey over a period of 30 min, neither did Knoop microhardness tests s...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90110-4
更新日期：1994-02-01 00:00:00
abstract：:Age-related changes in the content of non-reducible crosslink amino acids, pyridinoline and histidinoalanine, in rat mandibular bone were studied. The pyridinoline content markedly increased up to 12 months of age and thereafter slightly increased. The histidinoalanine content was low until 3 months of age, but therea...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90075-5
更新日期：1985-01-01 00:00:00
abstract：:Two experimental approaches were used to examine the relationship between c-fos gene expression and tissue proliferative responses. Beta-Adrenergic and muscarinic receptor stimulation yielded equivalent levels of c-fos expression, although only beta-adrenergic receptor agonists are reported as capable of eliciting DNA...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90088-5
更新日期：1989-01-01 00:00:00
abstract：:Using a computerized technique the bone height and prevalence of approximal periodontal intrabony defects were assessed on posterior bite-wing radiographs from 151 young adults. The results were related to the buffering capacity and counts of Streptococcus mutans and lactobacilli in whole stimulated saliva. The mean d...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90161-3
更新日期：1990-01-01 00:00:00
abstract：:Previous laboratory studies have revealed that some axons branch to supply the pulps of two teeth, but the incidence of such fibres in different regions of the jaws has not been investigated. The present study has used electrophysiological techniques to determine the incidence and distribution of branched pulpal axons...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90109-y
更新日期：1993-11-01 00:00:00
abstract：:Herpes simplex virus type 1 (HSV1) can be rapidly identified in saliva from patients with acute herpetic gingivostomatitis, by in vitro amplification using the polymerase chain reaction and specific primers. Amplification of DNA results in a product of 110 bp length corresponding to the region 1381-1490 bp of the HSV1...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90113-m
更新日期：1992-10-01 00:00:00
abstract：:Cementum of teeth previously analysed for fluoride was re-examined in order to determine whether or not periodontal disease had affected the thickness of the tissue. In the periodontally diseased teeth the cementum was thinner than normal in the middle region of the root. The apical cementum, however, was significantl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90132-r
更新日期：1992-08-01 00:00:00
abstract：:In the majority of studies on masticatory performance, the degree of food breakdown has been determined by sieving the food with one or more sieves. The aim of the present study was to compare the results obtained with a single and a multiple sieve method, as these methods have never been compared. We analysed the che...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2003.08.007
更新日期：2004-03-01 00:00:00
abstract：:Specific, well-characterized antisera to the extracellular matrix glycoprotein tenascin were used. Immunoreactivity was detected in association with dentinal tubules; it was particularly prominent in the tooth crown, and was stronger within matrix than within predentine. In contrast, there was no anti-fibronectin stai...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90080-e
更新日期：1991-01-01 00:00:00
abstract：:After an initial demineralisation, an intensive fluoridation is capable of inhibiting the erosive mineral loss in dentine completely, which might be related to the presence of the exposed organic dentine matrix. Aim of the present study was, therefore, to evaluate whether fluoride is also effective when the organic ma...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.05.010
更新日期：2004-11-01 00:00:00
abstract：:In 14 ovariectomized and 15 control rats, killed at intervals over a 117-day period, the height of the mandible in the molar region was determined radiographically. Mandibular height was significantly related to time after operation in both groups, but their rate of mandibular growth was not significantly different. I...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90110-v
更新日期：1990-01-01 00:00:00
abstract：:Perfusion with a variety of commonly-used fixatives was used. Quality of cell preservation and occurrence of stippled material between the mineralization front and the secretory ameloblasts were compared in normal animals and in ones injected with fluoride. There was no relationship between the formation of stippled m...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90172-9
更新日期：1984-01-01 00:00:00
abstract：:The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that M...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90070-6
更新日期：1985-01-01 00:00:00
abstract：:The EMG patterns of temporalis, masseter and digastric muscles of twenty Wistar white rats were studied as they ate large and small standard food pellets, bread and pudding. Bipolar EMG electrodes were placed in the muscles and led subcutaneously to a connector pedestal on the rat's head. Integrated records of the EMG...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90090-0
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVE:Dental follicle cells (DFCs) are the genuine precursors of alveolar osteoblasts. Previous studies suggested that collagen I supports the osteogenic differentiation of DFCs. This study investigated the effect of collagen I on the osteogenic differentiation of human DFCs. MATERIALS AND METHODS:We modified the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.07.013
更新日期：2014-12-01 00:00:00
abstract：:Nicotine, a major component in tobacco smoke, stimulates the synthesis of prostaglandin E(2). We investigated the involvement of the transcription nuclear factor kappa B (NFkappaB) in the nicotine-induced expression of cyclooxygenase-2 (COX-2), a key enzyme for prostaglandin synthesis, in human gingival fibroblasts. N...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.11.006
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVE:The neonatal line (NNL) is in principle found in all primary teeth and the line represents the time of birth. Earlier findings of the appearance of the NNL in light microscope and in microradiographs have shown not only changes in the prism direction of the enamel, but that the NNL has a hypomineralized chara...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.05.003
更新日期：2008-10-01 00:00:00
