Abstract:
:The discovery of new metal-selective complexing agents may be facilitated by applying an in vitro selection strategy. Such a strategy was recently devised to identify and enrich populations of bacteriophage that rely on Mg(II)-, Zn(II)-, or Au(III)-selective stabilization for survival in the presence of denaturing urea. The potential for extension of the strategy to other metal ions is investigated here. The kinetics of phage inactivation in 5 M urea was measured for a spectrum of metals. At a concentration of 1 mM, Mg(II), Ca(II), Co(II), and Ni(II) were found to be the most stabilizing, followed by Cd(II), Cu(II), and Zn(II), respectively. K(I) had virtually no effect. In contrast, Al(III) and Au(III) significantly destabilized the phage, even at concentrations of 0.25 mM.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Whang T,Daly B,Yin Jdoi
10.1016/0162-0134(96)85609-1subject
Has Abstractpub_date
1996-07-01 00:00:00pages
1-7issue
1eissn
0162-0134issn
1873-3344pii
0162013496856091journal_volume
63pub_type
杂志文章abstract::The study aimed to develop simple assays to study aluminium-ligand interactions in natural/biological systems where equilibrium is rarely reached and thus where the initial seconds or hours of interactions are important. The immediate and non-equilibrium precipitation of aluminium hydroxide, in aqueous solution at neu...
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章,评审
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
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abstract::Oxaliplatin-based chemotherapy is the mainstay for the treatment of advanced colorectal cancer. Copper transporter proteins have been implicated in the transport of platinum-based anticancer drugs, but their expression in human colorectal cancer cell lines and roles in controlling their sensitivity to oxaliplatin are ...
journal_title:Journal of inorganic biochemistry
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