Abstract:
:It has been shown that glucocorticoids accelerate lung development by limiting alveolar formation resulting from a premature maturation of the alveolar septa. Based on these data, the aim of the present work was to analyze the influence of dexamethasone on cell cycle control mechanisms during postnatal lung development. Cell proliferation is regulated by a network of signaling pathways that converge to the key regulator of cell cycle machinery: the cyclin-dependent kinase (CDK) system. The activity of the various cyclin/CDK complexes can be modulated by the levels of the cyclins and their CDKs, and by expression of specific CDK inhibitors (CKIs). In the present study, newborn rats were given a 4-d treatment with dexamethasone (0.1-0.01 microg/g body weight dexamethasone sodium phosphate daily on d 1-4), or saline. Morphologically, the treatment caused a significant thinning of the septa and an acceleration of lung maturation on d 4. Study of cyclin/CDK system at d 1-36 documented a transient down-regulation of cyclin/CDK complex activities at d 4 in the dexamethasone-treated animals. Analysis of the mechanisms involved suggested a role for the CKIs p21CIP1 and p27KIP1. Indeed, we observed an increase in p21CIP1 and p27KIP1 protein levels on d 4 in the dexamethasone-treated animals. By contrast, no variations in either cyclin and CDK expression, or cyclin/CDK complex formation could be documented. We conclude that glucocorticoids may accelerate lung maturation by influencing cell cycle control mechanisms, mainly through impairment of G1 cyclin/CDK complex activation.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Corroyer S,Schittny JC,Djonov V,Burri PH,Clement Adoi
10.1203/00006450-200202000-00008subject
Has Abstractpub_date
2002-02-01 00:00:00pages
169-76issue
2eissn
0031-3998issn
1530-0447journal_volume
51pub_type
杂志文章abstract::Chitosan is a natural, cationic polysaccharide derived from fully or partially deacetylated chitin. Chitosan is capable of inducing large phospholipid aggregates, closely resembling the function of nonionic polymers tested previously as additives to therapeutic lung surfactants. The effects of chitosan on improving th...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000227558.14024.57
更新日期:2006-08-01 00:00:00
abstract::We investigated whether obese children and adolescents have early echocardiographic signs of subclinical cardiac dysfunction and evaluated the respective influence of obesity per se versus parameters of carbohydrate and lipid metabolism that are frequently abnormal in obese subjects. The role of tissue Doppler imaging...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e318176182b
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:Pulmonary hypertension (PH) in patients with bronchopulmonary dysplasia (BPD) results from vasoconstriction and/or vascular remodeling, which can be regulated by mitogen-activated protein kinases (MAPKs). MAPKs are deactivated by dual-specificity phosphatases (DUSPs). We hypothesized that single-nucleotide p...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0502-9
更新日期:2020-01-01 00:00:00
abstract::The interactions between CD40 and CD40L (CD154) are critical for effective humoral immune response. CD40 signaling facilitates T lymphocyte dependent B cell proliferation and immunoglobulin isotype switch. The objective of our study was to investigate the CD40 and CD40L expression on peripheral blood mononuclear cells...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000219298.96471.18
更新日期:2006-06-01 00:00:00
abstract::The present study employed an animal model in an attempt to document effects on brain growth of long-term exposure to theophylline in the presence or absence of malnutrition. One group of dams was randomly divided into half. One-half was fed a 20% protein diet and the other half a 6% protein diet. Similarly, a second ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198406000-00014
更新日期:1984-06-01 00:00:00
abstract::Our goal for this study was to determine whether the maturation of fat absorption in neonatal life is functionally related to an increased ability to hydrolyze dietary fat, to absorb long-chain fatty acids, or to do both. In 16 preterm and in eight term neonates, the intestinal ability to hydrolyze triacylglycerols an...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200201000-00011
更新日期:2002-01-01 00:00:00
abstract::To determine whether human milk contained soluble receptors and cytokine antagonists that might contribute to its anti-inflammatory properties, ELISA and enzyme-amplified sensitivity immunoassay methods were used to quantitate soluble intercellular and vascular cell adhesion molecules, soluble E-selectin, soluble IL-6...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199612000-00011
更新日期:1996-12-01 00:00:00
abstract::The head sizes of 38 patients, growth hormone (GH) deficient following craniospinal (n = 26) or cranial irradiation (n = 12), have been assessed before (n = 38) and on completion of GH therapy (n = 15) or at the end of a similar period of observation without GH (n = 7). These results were compared to the change in hea...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198710000-00007
更新日期:1987-10-01 00:00:00
abstract:BACKGROUND:The aim was to identify susceptibility alleles for infantile hypertrophic pyloric stenosis (IHPS) in a pedigree previously linked to IHPS5 on chromosome 16q24. METHODS:We screened the positional and functional candidate gene FOXF1 by Sanger sequencing in a single affected individual. All family members for ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2016.244
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:The preterm newborn is at high risk of developing cardiovascular compromise during the first day of life and this is associated with increased risk of brain injury. Standard treatments are volume expansion and administration of inotropes, typically dopamine and/or dobutamine, but there is limited evidence th...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2016.156
更新日期:2016-12-01 00:00:00
abstract::Infants fed soy-based infant formulas are exposed to high levels of genistein, an isoflavone, with potential estrogen-like activity. This study determined whether neonatal exposure of mice to genistein resulted in higher bone mineral density (BMD) and greater resistance to fracture at adulthood. Male and female CD-1 m...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000250200.94611.03
更新日期:2007-01-01 00:00:00
abstract::Intestinal absorption and its relationship to increased total body water was studied in seven infants with congenital heart disease receiving regular diuretics due to congestive heart failure. All infants and six age-matched healthy controls were studied for a 3-d period during which time all food intake was recorded,...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-199410000-00023
更新日期:1994-10-01 00:00:00
abstract:BACKGROUND:Scoring tools used to quantify withdrawal in infants with neonatal abstinence syndrome (NAS) are often confounded by subjective measurements. This study assessed salivary cortisol as an objective biomarker of withdrawal severity in opioid-exposed newborns. METHODS:A prospective study was conducted in 25 ful...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0601-7
更新日期:2020-05-01 00:00:00
abstract::In its normal circulatory environment, the fetal left ventricle can maximally increase output less than 2-fold, in contrast to the nearly 3-fold increase that occurs at birth. Several studies have attributed this finding to fetal myocardial "immaturity," and speculated that there is a rapid maturation of the myocardiu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199105010-00011
更新日期:1991-05-01 00:00:00
abstract::The immunological competence of neonatal rabbits inoculated intradermally with Treponema pallidum was examined. Both cellular responses and the production of humoral antibody to specific T. pallidum antigens and to nonspecific antigens or mitogens were investigated. In blast transformation assays, splenic and poplitea...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198410000-00013
更新日期:1984-10-01 00:00:00
abstract::Patency of the ductus arteriosus in preterm infants is mediated by vasodilating prostanoids; however, reliable methods to monitor prostanoid activity or production in preterm infants are lacking. We measured the excretion rates of major and characteristic urinary metabolites of prostacyclin (PGI2), PGE1, and PGE2, 6-k...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198406000-00007
更新日期:1984-06-01 00:00:00
abstract::We determined the inhibitory effects of concentrations of tin- and zinc protoporphyrin (1-100 microM) and mesoporphyrin (0.1-10 microM) on the in vitro heme oxygenase (HO) (E.C.1.14.99.3) activity in liver, spleen, and intestine 13,000 x g tissue supernatants from fasted adult male Wistar rats through measurement of c...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198910000-00015
更新日期:1989-10-01 00:00:00
abstract::Concanavalin A surface receptors were detected on brush border enterocytes from child intestinal biopsies by the concanavalin A-peroxidase method. On electron microscopy, these receptors appeared as electron-dense deposits, located on a band running along the innermost part of the brush border membrane glycocalyx. In ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198206000-00008
更新日期:1982-06-01 00:00:00
abstract::Defective arousal mechanisms are viewed as contributory to sleep hypopnea disorders and sudden infant death syndrome. Sighs (i.e. augmented breaths) as well as startles have not traditionally been viewed as arousal-related phenomena in infants. We hypothesized that, if sighs and startles are the initial event in a seq...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199811000-00021
更新日期:1998-11-01 00:00:00
abstract::Phototherapy results in the conversion of bilirubin to more water-soluble isomers. Six clinically used phototherapy lamps which differ in their emission spectra have been compared in their ability to produce configurational and structural isomers of bilirubin in vitro. For all of the lamps, the initial rate of configu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198407000-00021
更新日期:1984-07-01 00:00:00
abstract::Injuries continue to be the leading cause of morbidity and mortality for children, adolescents, and young adults aged 1-24 years in industrialized countries in the twenty-first century. In this age group, injuries cause more fatalities than all other causes combined in the United States (U.S.). Importantly, many of th...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/s41390-019-0549-7
更新日期:2020-01-01 00:00:00
abstract::We used in vivo microdialysis to determine the impact of a focal hypoxic-ischemic insult on hippocampal amino acid efflux in the immature brain. Microdialysis probes were inserted into the right hippocampus of postnatal d 7 rats. To induce hypoxic-ischemic injury, the right carotid artery was ligated and animals were ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199112000-00021
更新日期:1991-12-01 00:00:00
abstract::To evaluate the maturation of glucose homeostasis in the small for gestational age (SGA) neonate, glucose kinetics were measured with 78% enriched D-[U-13C]glucose by the prime plus constant infusion technique in nine SGA infants and compared with the rate obtained in seven term appropriate for gestational age infants...
journal_title:Pediatric research
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Obese children have a great risk of hypertension and cardiovascular morbidity in adults. The insulin-like growth factor type II (IGF-II) regulates glucose homeostasis, cardiovascular functions, and lipid metabolism. IGF2 gene variants have shown a strong association with weight, body mass index (BMI), and metabolic pr...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181d22757
更新日期:2010-04-01 00:00:00
abstract::A technique is described in which paper chromatoelectrophoresis is used to measure the bilirubin binding capacity of albumin in serum. It is equivalent to the Sephadex G-25 method and its results are highly reproducible. From a single pool of serum, multiple determinations indicated the mean bilirubin binding capacity...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197707000-00006
更新日期:1977-07-01 00:00:00
abstract::BackgroundPatent ductus arteriosus (PDA) is a common complication seen in preterm infants. Indomethacin is routinely used to treat PDA. Evidence suggests that the response of indomethacin is highly heritable. This study investigated the association between single-nucleotide polymorphisms (SNPs) in CYP2C9 and the closu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2017.145
更新日期:2017-11-01 00:00:00
abstract::In the small bowel of patients with cystic fibrosis, primary defects involving both chloride transport and mucus secretion have been demonstrated, but there is no general consensus about the morphologic counterpart of functional and biochemical abnormalities. We have studied the intestinal mucosa in a group of patient...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199802000-00013
更新日期:1998-02-01 00:00:00
abstract::There is increasing evidence that cortisol contributes to cardiovascular risk. It is unclear whether physiological concentrations of serum cortisol are related to vascular risk markers in children. The cross-sectional associations between morning serum cortisol and cardiovascular risk markers: blood pressure (BP) and ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181efc310
更新日期:2010-11-01 00:00:00
abstract::Preterm infants are more prone to bone mineral deficiency the lower their birth weight. To achieve the intrauterine bone mineral accretion rate postnatally, 74 low-birth-weight infants (median birth weight, 980 g; range 430-1.580 g) were each supplemented enterally and/or parenterally with calcium and/or phosphorus in...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-199401000-00027
更新日期:1994-01-01 00:00:00
abstract::Sex steroids accelerate bone maturation, but it is believed that estrogen action is needed for terminal epiphyseal fusion. In this study, we investigated the effects of a new estrogen-blocking agent, Faslodex (ICI 182,780), on estrogen-accelerated skeletal maturation in immature mice. On day-of-life 2 through 8, mice ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199909000-00004
更新日期:1999-09-01 00:00:00