Abstract:
:Platelet-activating factor (PAF) has been implicated in the pathogenesis of gastrointestinal diseases such as necrotizing enterocolitis, Crohn's disease, and ulcerative colitis. However, neither the physiologic role of PAF in the intestine, nor the mechanisms by which PAF participates in the pathogenesis of disease are well understood. The aim of the present study was to determine the direct effect of PAF on intestinal epithelial cell ion transport, and to delineate the mechanisms of regulation. Ion transport was evaluated by measuring short circuit current (I(sc)) in HT29-CL19A cell monolayers using Ussing chambers. PAF receptor polarity was assessed using domain-selective biotinylation followed by immunoprecipitation and streptavidin blotting of intact epithelial monolayers. PAF (1-200 microM) stimulated I(sc) that followed the direction of a Cl(-) gradient and was specifically inhibited by the Cl(-) channel blockers glybenclamide, 2,2' iminodibenzoic acid and 4,4' diisothiocyanostilbene-2, 2' disulfonic acid, but was unaffected by the inhibition of prostaglandin synthesis with indomethacin. Stimulated I(sc) was only detected after apical addition of PAF, correlating with the results of biotinylation experiments indicating an exclusive apical polarity of the PAF receptor. PAF receptor antagonists CV6209 and octylonium bromide abolished PAF-stimulated I(sc). Thus, mucosal acting PAF directly and specifically stimulates ion transport via activation of an apical Cl(-) channel in intestinal epithelial cell monolayers independent of prostaglandin biosynthesis.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Claud EC,Li D,Xiao Y,Caplan MS,Jilling Tdoi
10.1203/00006450-200208000-00005subject
Has Abstractpub_date
2002-08-01 00:00:00pages
155-62issue
2eissn
0031-3998issn
1530-0447journal_volume
52pub_type
杂志文章abstract::Prolonged unconjugated hyperbilirubinemia in infants associated with breast milk feeding is a common pediatric problem known as breast milk jaundice (BMJ). A polymorphic mutation (G71R) of bilirubin UDP-glucuronosyltransferase (UGT1A1) is a known cause of BMJ on the infantile side, but the responsible components of br...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31822f242e
更新日期:2011-11-01 00:00:00
abstract::Long chain polyunsaturated fatty acids (LCPUFA)seem to be the most trophic macronutrients in inducing intestinal adaptation in adult short bowel syndrome (SBS), although their effects on intestinal adaptation in infants with SBS remain unknown.It is hypothesized that a high fat diet enriched with n-3 LCPUFA derived fr...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181eb2ee5
更新日期:2010-09-01 00:00:00
abstract::Patency of the ductus arteriosus in preterm infants is mediated by vasodilating prostanoids; however, reliable methods to monitor prostanoid activity or production in preterm infants are lacking. We measured the excretion rates of major and characteristic urinary metabolites of prostacyclin (PGI2), PGE1, and PGE2, 6-k...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198406000-00007
更新日期:1984-06-01 00:00:00
abstract:BACKGROUND:Fetal hypoxia-ischemia (H-I) results in significant morbidity and mortality. Little is known about the timing of death in human stillbirths. The vulnerability of the fetus varies with age at the time of insult, but it is unknown what happens to the timing of fetal death in relation to a fetal insult. We aske...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.65
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND:We examined the extent of fetal lung hypoplasia and lung maturation using the amniotic lamellar body count (LBC) in congenital diaphragmatic hernia (CDH). METHODS:We obtained 30 amniotic fluid samples from human CDH cases during cesarean section. We assessed LBC, magnetic resonance imaging (MRI), and ultras...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.187
更新日期:2013-03-01 00:00:00
abstract::In mice, secondary alveolar septal formation primarily occurs during a brief postnatal period and is accompanied by transient expansion of the interstitial lung fibroblast (LF) population. PDGF-A, which solely signals through PDGF-receptor-alpha (PDGF-Rα), is required for expansion, but the receptor's relevant downstr...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31821cfb5a
更新日期:2011-07-01 00:00:00
abstract::To examine the levels and relationship of blood pressure and insulin during puberty, blood pressure and serum insulin were measured in 3596 subjects, aged 3-18 y, whose pubertal status was graded according to the Tanner classification. The same study protocol was repeated in two follow-up surveys 3 and 6 y later for 2...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1203/00006450-199702000-00019
更新日期:1997-02-01 00:00:00
abstract::Premature newborns are highly susceptible to severe bacterial infections. This is partially due to their immature innate immune system, characterized by decreased neutrophil and monocyte activity as well as by reduced concentrations of complement factors. However, additional mechanisms might be important for innate im...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000163397.53466.0F
更新日期:2005-07-01 00:00:00
abstract::The mitochondrial DNA (mtDNA) point mutation T8993G has been associated with maternally inherited Leigh syndrome (MILS) when very abundant (> 95%). MILS patients are usually severely affected and die in early infancy. In 1993, a novel T8993C point mutation was described in a juvenile form of Leigh syndrome (LS) charac...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199605000-00028
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:To investigate relationships between infant body composition (BC) and human milk (HM) immunomodulatory proteins (IMPs) during the first 12 months of lactation. METHODS:BC of breastfeeding dyads (n = 20) was measured with ultrasound skinfolds (infants) and bioimpedance spectroscopy (infants/mothers) at 2, 5,...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-0961-z
更新日期:2020-05-21 00:00:00
abstract:BACKGROUND:Cardiovascular instability immediately after birth is associated with intraventricular hemorrhage (IVH) in very-low-birth-weight (VLBW) infants. For circulatory management, evaluation of organ blood flow is important. In this study, the relationship between peripheral perfusion within 48 h after birth and IV...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.215
更新日期:2014-02-01 00:00:00
abstract::We have shown that vitamin A (VA) and retinoic acid (RA) synergistically increase lung retinyl ester content in neonatal rats. To confirm whether this biochemical synergism attenuates early neonatal hyperoxic lung injury in mice, we exposed newborn C57BL/6 mice to 95% O2 or air from birth to 4 d. The agent [vehicle, V...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181dbac3d
更新日期:2010-06-01 00:00:00
abstract::Hereditary surfactant protein B (SP-B) deficiency has been lethal in the first year of life without lung transplantation. We tested the hypothesis that SP-B gene mutations may result in milder phenotypes by investigating the mechanisms for lung disease in two children with less severe symptoms than have been previousl...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200009000-00003
更新日期:2000-09-01 00:00:00
abstract::Several lines of evidence suggest that formula with protein content of 3.0 g/100 kcal does not fully meet the protein needs of very-low-birth weight infants. Our purpose was to compare nitrogen balance, metabolic status and growth in infants fed a standard (3.0 g/100 kcal; RegPro) and high (3.6 g/100 kcal; HiPro) prot...
journal_title:Pediatric research
pub_type: 杂志文章,随机对照试验
doi:10.1203/01.pdr.0000196376.99101.34
更新日期:2006-02-01 00:00:00
abstract::The purpose of the study was to investigate the effect of low doses of dopamine in children. Fourteen cases were studied after open heart surgery. Cardiac output and renal parameters were determined under baseline conditions and under continuous infusion of dopamine 2.5 and 5 micrograms/kg/min. During the control peri...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198909000-00009
更新日期:1989-09-01 00:00:00
abstract::The present study employed an animal model in an attempt to document effects on brain growth of long-term exposure to theophylline in the presence or absence of malnutrition. One group of dams was randomly divided into half. One-half was fed a 20% protein diet and the other half a 6% protein diet. Similarly, a second ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198406000-00014
更新日期:1984-06-01 00:00:00
abstract::Chronic fetal anemia causes polyhydramnios and fetal hydrops and is associated with increased fetal diuresis and natriuresis. To determine the role of atrial natriuretic peptide (ANP) in the renal adaptation to chronic fetal anemia we studied the effects of HS-142-1 (HS), a specific inhibitor of the guanylate cyclase-...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199511000-00015
更新日期:1995-11-01 00:00:00
abstract::To examine uridine diphosphate hexose (UDPhexose) content of cells that have more complete metabolic patterns than erythrocytes, which have been commonly used in the study of galactosemia, the concentrations of uridine diphosphate galactose (UDPgalactose) and uridine diphosphate glucose (UDPglucose) were determined in...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199411000-00014
更新日期:1994-11-01 00:00:00
abstract::Keratinocyte growth factor (KGF, or fibroblast growth factor 7) was previously reported to enhance the synthesis of surfactant in alveolar type II cells. We investigated the possible interactions between KGF and a glucocorticoid, dexamethasone (Dex), on surfactant protein (SP) gene expression. In cultured fetal rat ty...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000047840.77635.AE
更新日期:2003-02-01 00:00:00
abstract::Serum copper (Cu) and zinc (Zn) concentrations were measured in neonates with an appropriate birth weight for gestational age (AGA) and in small-for-gestational age infants. At 7 days of age, there was a positive correlation between serum Cu concentration and gestational age (GA) (r = 0.63; P < 0.001) and a negative c...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198009000-00005
更新日期:1980-09-01 00:00:00
abstract:BACKGROUND:The pathogenesis of BPD includes inflammation and oxidative stress in the immature lung. Corticosteroids improve respiratory status and outcome, but the optimal treatment regimen for benefit with low systemic effects is uncertain. METHODS:In a pilot dose escalation trial, we administered ≤5 daily doses of b...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-01343-z
更新日期:2021-01-19 00:00:00
abstract::The relationship between the development of cytotoxic cellular immune response to herpes simplex virus type I (HSV-1)-infected autologous cells and the production of interferon (IFN) was studied using in vitro secondary sensitization of peripheral blood leukocytes in subjects with recurrent herpes labialis (RHL) and i...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198609000-00020
更新日期:1986-09-01 00:00:00
abstract::In Chinese children with steroid-resistant nephrotic syndrome (SRNS), it was reported that NPHS2 mutation was detected in 4.3%, which was lower than that in Caucasians (10-30%). However, there were no data on WT1 mutation in nephrotic syndrome (NS), especially in early-onset NS of Chinese children. Thus, a study, whic...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181e4c9e3
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Intrauterine growth restriction (IUGR) has been linked to heart disease in adulthood. Hence the IUGR heart is likely to be vulnerable to diabetic heart disease. The aim of this study was to examine the effect of induction of type 1 diabetes on myocardial collagen deposition and cardiac function in adult rats...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.94
更新日期:2012-10-01 00:00:00
abstract::We examined adult sheep lymphocytes for the following surface markers: Surface membrane immunoglobulin, antigen identified by anti-sheep thymocyte globulin and complement receptors. We quantitated sheep peripheral blood mononuclear leukocyte proliferative responses to mitogens and alloantigens, and demonstrated a neut...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198405000-00012
更新日期:1984-05-01 00:00:00
abstract:BACKGROUND:Prolonged hypoglycemia leads to brain injury, despite treatment with 10% dextrose. Whether induction of hyperglycemia or ketonemia achieves better neuroprotection is unknown. Hyperglycemia is neuroprotective in other brain injuries during development; however, it worsens hypoglycemia-induced injury in the ad...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2014.146
更新日期:2015-01-01 00:00:00
abstract::Docosahexaenoic acid (DHA) is an omega-3 fatty acid that is highly concentrated in CNS tissues. Although breast milk contains the fatty acids DHA and arachidonic acid, infant formulas marketed in North America do not contain these nutrients. The potential deleterious effects of rearing infants with formulas devoid of ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200203000-00003
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Social determinants of health (SDH) can substantially impact health outcomes. A systematic review, however, has never been conducted on associations of SDH with congenital heart disease (CHD) outcomes. The aim, therefore, was to conduct such a systematic review. METHODS:Seven databases were searched through...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/s41390-020-01196-6
更新日期:2020-10-17 00:00:00
abstract::Our aim was to compare white matter (WM) microstructure in preterm infants with and without punctate WM lesions on MRI using tract-based spatial statistics (TBSS) and probabilistic tractography. We studied 23 preterm infants with punctate lesions, median GA at birth 30 (25-35) wk, and 23 GA- and sex-matched preterm co...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3182182836
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Staphylococcus epidermidis (SE) is an important cause of late-onset sepsis in neonates. SE frequently produces a polysaccharide intercellular adhesin (PIA) biofilm, important in the pathogenesis of these infections. Little is known about how the neonatal innate immune system reacts to SE biofilm-associated i...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.193
更新日期:2013-03-01 00:00:00