Abstract:
OBJECTIVE:To investigate the characteristics and expression of transforming growth factor (TGF)-beta receptor subtypes on vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats. METHODS:The effects of TGF-beta 1 on DNA synthesis were evaluated by [3H]-thymidine incorporation into quiescent VSMC plated at high (5 x 10(4) cells/cm2) or low (5 x 10(3) cells/cm2) cell density. Specific binding of TGF-beta to VSMC was assessed by incubation of the cells with [125I]-TGF-beta 1. Affinity labelling of receptor subtypes was achieved by exposure of the cells to [125I]-TGF-beta 1 and cross-linking with disuccimidyl suberate. RESULTS:VSMC from SHR displayed a biphasic DNA synthesis response to TGF-beta 1 at high cell density, with DNA synthesis stimulated by low concentrations of TGF-beta 1 but not by high concentrations, whereas at low cell density there was a small increase in DNA synthesis in response to TGF-beta 1. TGF-beta 1 inhibited DNA synthesis in VSMC from WKY rats at both high and low cell densities. Binding assays revealed that VSMC from SHR had a larger number of TGF-beta receptors and a higher affinity for TGF-beta at high and at low cell densities. The affinity labelling with [125I]-TGF-beta 1 revealed the presence of receptor subtypes with relative molecular masses of 280-300, 85, 70, 60 and 50 x 10(3) on vascular smooth muscle cells from both rat strains at high cell density. The abundance of the 85 x 10(3) molecular mass receptor subtype was greater in VSMC from SHR. The 85 x 10(3) molecular mass receptor subtype was not detected on VSMC from either strain at low cell density. CONCLUSION:The present results suggest a different expression of TGF-beta receptor subtypes on VSMC from SHR and WKY rats. These differences may account for the exaggerated proliferative response of VSMC from SHR to TGF-beta.
journal_name
J Hypertensjournal_title
Journal of hypertensionauthors
Fukuda N,Kubo A,Izumi Y,Soma M,Kanmatsuse Kdoi
10.1097/00004872-199508000-00003subject
Has Abstractpub_date
1995-08-01 00:00:00pages
831-7issue
8eissn
0263-6352issn
1473-5598journal_volume
13pub_type
杂志文章abstract:OBJECTIVE:We examined the relationship between brachial-ankle pulse wave velocity and the development of cardiovascular disease in a general Japanese population. METHODS:A total of 2916 community-dwelling Japanese individuals without history of cardiovascular disease aged at least 40 years were followed up for an aver...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e32835c5c23
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVE(S):To study the changes in macrophage inducible nitric oxide synthase (iNOS) activity, plasma levels of nitrite, lipid peroxidation (LPO) and melatonin in human essential hypertension before and 6 months after 4 mg/day lacidipine treatment. DESIGN:The study was carried out in a total of 25 subjects--11 healt...
journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章
doi:10.1097/00004872-200403000-00027
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:The aldosterone synthase gene (CYP1B2) locus is a candidate region involved in the development of hypertension. OBJECTIVE:To study the relationship between the C-344T CYP1B2 polymorphism, plasma aldosterone, renin activity and blood pressure in a multi-ethnic population. DESIGN:Population-based, cross-sect...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-200410000-00011
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVE:We attempted to determine a valid pharmacological approach for the control of high blood pressure in obese hypertensive patients using two antihypertensive agents with different pharmacological actions. DESIGN:Thirty-two obese hypertensive black women were randomly treated with chlorthalidone or clonidine to...
journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004872-199205000-00013
更新日期:1992-05-01 00:00:00
abstract:OBJECTIVE:We investigated the impact of renal function on outcomes after percutaneous transluminal angioplasty. METHODS:A total of 139 hypertensive patients with atherosclerotic renal artery stenosis (mean age, 70 years, 80.6% men) who underwent renal percutaneous transluminal angioplasty were included. Renal function...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001499
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:To assess the effect of orlistat plus diet compared with diet alone in promoting weight loss and blood pressure reduction in hypertensive, overweight/obese patients. DESIGN:A pragmatic randomized, controlled trial. SETTING:Hypertension clinic of a university hospital. PATIENTS:Hypertensive patients aged 18...
journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004872-200311000-00026
更新日期:2003-11-01 00:00:00
abstract:OBJECTIVES:Genetic variants that influence large conductance calcium-activated potassium channel's function may alter arterial function and contribute to the known heritability of arterial stiffness and blood pressure. The beta1-subunit (KCNMB1) of the large conductance calcium-activated potassium channel includes two ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e328317c8ae
更新日期:2009-01-01 00:00:00
abstract:UNLABELLED:PURPOSE IDENTIFICATION: Inter-individual variability in blood pressure response to treatment is well documented, but a clinically useful means to distinguish responders from non-responders has been elusive. With the advent of new technologies and genomic knowledge, more investigators are seeking to identify ...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/01.hjh.0000173510.52987.68
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVE:We have previously reported that hearts from N-[omega]-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats exhibited an enhanced expression of Gi proteins. Since, losartan, an AT1 receptor antagonist, has been shown to attenuate the L-NAME-induced increase in blood pressure, we undertook the pres...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-200401000-00028
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Previous work has demonstrated that treatment of hypertensive patients with the angiotensin-converting enzyme inhibitor lisinopril was associated with a reduced incidence of a composite conduction system disease endpoint and also left bundle branch block (LBBB) compared with chlorthalidone therapy. The relat...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001915
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:To test whether angiotensin II (Ang II) through the Ang II type 2 receptor (AT2R), downregulates RhoA/Rho kinase, which plays a role in AT1 receptor (AT1R)-mediated function. METHODS:In vitro studies were performed in A10 vascular smooth muscle cells (VSMC) and in vivo studies in mesenteric arteries from Wis...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000166845.49850.39
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVES:Urocortin 1 (Ucn1) and the sympathetic nervous system both participate in cardiac and circulatory regulation, but little is known about their possible interactions. METHODS:We report the effects of Ucn1 on the cardiac sympathetic nerve activity (CSNA), haemodynamics and plasma catecholamines in normal sheep...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3282f01428
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVES:Arterial stiffness is associated with excess morbidity and mortality, independently of other cardiovascular risk factors. Age is the main determinant responsible for arterial wall changes leading to arterial stiffening. Environmental and genetic factors may however influence the magnitude of the effects of a...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-200103000-00008
更新日期:2001-03-01 00:00:00
abstract:OBJECTIVE:To test the hypothesis that vascular beta 2-adrenoceptor hyporesponsiveness in spontaneously hypertensive rats (SHR) is not induced by increased blood pressure or venous hypertrophy. DESIGN:We compared the attenuating effects or isoprenaline, sodium nitroprusside and verapamilon the portal veins from Wistar-...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199509000-00013
更新日期:1995-09-01 00:00:00
abstract:OBJECTIVE:The physiopathological mechanisms implicated in hypertensive heart disease are multi-factorial, including myocyte hypertrophy, apoptosis and myocardial remodelling. In this process, some hormonal and local growth factors have a regulatory influence. The aim of this study was to evaluate the potential role of ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000493
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVE:Recent studies implied that angiotension receptor blockers (ARBs) not only have an antihypertensive effect but also have beneficial effects on dementia. The purpose of this study was to investigate the effects of ARBs on dementia and the subtypes. METHODS:We conducted a population-based cohort study with dat...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000086
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:A recent editorial published by Verma and Strauss, entitled 'Angiotensin receptor blockers and myocardial infarction', examined, through a partial analysis of individual trials, the use of angiotensin receptor blockers (ARBs) in a variety of clinical settings. This editorial was reported widely in the lay pr...
journal_title:Journal of hypertension
pub_type: 杂志文章,meta分析,评审
doi:10.1097/01.hjh.0000194114.12228.16
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVE:Recent studies have demonstrated that stimulating autoantibodies against the angiotensin II type 1 receptor (AT1R-AA) are frequently detected in the sera from women with preeclampsia, suggesting that they may play an important role in the pathogenesis of this disease. Nevertheless, the real clinical significa...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3283376cc6
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVES:Visit-to-visit variability of blood pressure (VVV of BP) is an important independent risk factor for premature death and cardiovascular events, but relatively little is known about this phenomenon in patients with chronic kidney disease (CKD) not yet on dialysis. METHODS:We conducted a retrospective study i...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000779
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:Dipeptidyl peptidase-4 (DPP4) is a key protein in glucose homeostasis and a pharmacological target in type 2 diabetes mellitus. This study explored whether the novel adipokine soluble DPP4 (sDPP4) can cause endothelial dysfunction, an early marker of impaired vascular reactivity. METHOD:Reactivity was studi...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000886
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to examine the associations of birth outcomes and longitudinally measured fetal and infant growth patterns with retinal vessel calibers in childhood. METHODS:In a population-based prospective cohort study among 4122 children, we measured growth characteristics in second and third tri...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000561
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to evaluate the 24-h urinary sodium excretion in children of 10-12 years at a school in the north of Portugal and to examine the influence on salt intake and blood pressure of three different educational interventions for 6 months. METHODS:Blood pressure (BP) and sodium excretion in ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e328363572f
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Sitagliptin, a new antidiabetic drug that inhibits dipeptidyl peptidase (DPP)-4 enzyme activity, has been reported to possess neuroprotective property. We tested the protective effects of sitagliptin against chronic cerebral hypoperfusion (CHP) in mice after bilateral carotid artery stenosis (BCAS). METHOD:...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000529
更新日期:2015-05-01 00:00:00
abstract::Hypertension is a leading risk factor for mortality. Detecting, treating and monitoring hypertensive patients require accurate blood pressure measurements. Protocols for validation of automatic sphygmomanometers exist, but quality control of validations is limited to peer review of published reports, which has severe ...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/HJH.0b013e32833e1011
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of hypertension and diabetes mellitus on vascular albumin permeability and hypertrophy in 11-week-old Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) with and without 3 weeks of streptozotocin-induced diabetes. METHODS:Vascular albumin permeability was measured as...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199505000-00008
更新日期:1995-05-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to define the relationship between physical activity and the magnitude of the percentage fall in blood pressure at night (nocturnal dip). METHODS:We simultaneously monitored 24-h ambulatory blood pressure and measured physical activity by actigraphy in 434 patients. Blood pressure w...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-200018040-00008
更新日期:2000-04-01 00:00:00
abstract:INTRODUCTION AND OBJECTIVE:Blood pressure variability (BPV) within 24 h or between visits has been found to represent an independent risk factor for cardiovascular disease. The present study was aimed at determining whether a clinical significance can be given also to the BP variations occurring within a single clinica...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000700
更新日期:2015-11-01 00:00:00
abstract:AIM:To investigate same day repeated measures and diurnal variation of arterial stiffness, cardiac output (CO), stroke volume (SV) and total peripheral resistance (TPR) during the third trimester of normal pregnancy. METHODOLOGY:Pulse wave velocity (PWV) and augmentation index (AIx) were recorded using the Arteriograp...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001482
更新日期:2017-12-01 00:00:00
abstract::Endothelin-1 was infused into the non-dominant brachial artery in two male subjects. We then monitored intra-arterial mean blood pressure, right atrial pressure, the heart rate and forearm blood flow (by plethysmography). Endothelin-1 at a dose of 5 x 10(-14) to 5 x 10(-9) mol, infused over 5 min periods, elicited no ...
journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章
doi:10.1097/00004872-199009000-00004
更新日期:1990-09-01 00:00:00
abstract:OBJECTIVE:To determine whether obese hypertensive patients manifest a diminished regional vasodilator response to an oral glucose challenge. METHODS:Nineteen obese hypertensives (body mass index 34.1 +/- 1.3 kg/m2, blood pressure 144 +/- 3/96 +/- 2 mmHg) and 13 lean controls (23.6 +/- 0.3 kg/m2, 127 +/- 4/83 +/- 3 mmH...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00