Abstract:
:rho genes have been found in both lower and higher eucaryotes. They code for proteins of 21 kDa, highly conserved in evolution, which belong to the superfamily of ras GTPases. Among the members of this superfamily there are proteins with a regulatory function, such as ras, and proteins involved in vesicular trafficking, such as the family of rab proteins. We have investigated the putative role of rho proteins from Aplysia californica as transforming GTPases utilizing the wild-type and a Val-14 mutant, equivalent to the oncogenic Val-12 mutation of ras genes found in animal and human tumors. Over-expression of either rho gene was sufficient to confer anchorage- and serum-independent growth. Moreover, when introduced into nude mice, selected clones generated from either gene were able to induce tumors, although those carrying the mutated version were more efficient. Pathological analysis indicated that generated tumors corresponded to well-differentiated fibrosarcomas with distinct and intersecting bundles and spindle cells. By contrast, ras-induced tumors were poorly differentiated fibrosarcomas. Thus, our results indicate that under appropriate conditions rho genes function as oncogenes and may have a role in the regulation of proliferation in fibroblast cells.
journal_name
Oncogenejournal_title
Oncogeneauthors
Perona R,Esteve P,Jiménez B,Ballestero RP,Ramón y Cajal S,Lacal JCsubject
Has Abstractpub_date
1993-05-01 00:00:00pages
1285-92issue
5eissn
0950-9232issn
1476-5594journal_volume
8pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Abundant data support a key role for the transcription factor nuclear factor-kappaB (NF-kappaB) signaling pathway in controlling the initiation and progression of human cancer. NF-kappaB and associated regulatory proteins such as IkappaB kinase (IKK) are activated downstream of many oncoproteins and there is much evid...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1209942
更新日期:2006-10-30 00:00:00
abstract::Expression of polyomavirus middle-T antigen (middle-T) is involved in the formation of various tumors in vivo, e.g. hemangiomas and mammary gland tumors. Several genes have been shown to be activated in middle-T-expressing cells, but the underlying mechanisms have only been partially elucidated. Among the genes regula...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-12-07 00:00:00
abstract::Post-translational acetylation of lysines is most extensively studied in histones, but this modification is also found in many other proteins and is implicated in a wide range of biological processes in both the cell nucleus and the cytoplasm. Like phosphorylation, acetylation patterns and levels are often altered in ...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2014.453
更新日期:2015-09-17 00:00:00
abstract::The IGF2 gene, which encodes a growth factor, is subject to genomic imprinting. The frequently observed loss of IGF2 imprinting in a variety of tumors has been suggested to contribute to neoplasia. Since these reports have not documented the imprinting status of IGF2 at the cellular level, it cannot be excluded that t...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201501
更新日期:1998-01-08 00:00:00
abstract::Currently, trastuzumab resistance is a major clinical problem in the treatment of Her2-overexpressing breast cancer. The underlying molecular mechanisms are not fully understood. Our previous study demonstrates that β2-adrenergic receptor (β2-AR) and Her2 comprise a positive feedback loop in human breast cancer cells ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.58
更新日期:2016-01-07 00:00:00
abstract::The integrin linked kinase (ILK) is a cytoplasmic effector of integrin receptors, involved in the regulation of integrin binding properties as well as the activation of cell survival and proliferative pathways, including those involving MAP kinase, PKB/Akt and GSK-3beta. Overexpression of ILK in cultured intestinal an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204910
更新日期:2001-10-25 00:00:00
abstract::Apoptin has been described to induce apoptosis in various human cancer cell lines, but not in normal cells, thus making it an interesting candidate for the development of novel therapeutic strategies. Apoptin was generated and cloned into several mammalian expression vectors. Transfection or microinjection of apoptin ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207224
更新日期:2004-02-05 00:00:00
abstract::Different diagnostic and prognostic groups of colorectal carcinoma (CRC) have been defined. However, accurate diagnosis and prediction of survival are sometimes difficult. Gene expression profiling might improve these classifications and bring new insights into underlying molecular mechanisms. We profiled 50 cancerous...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207262
更新日期:2004-02-19 00:00:00
abstract::Inherited mutations in the BRCA1 gene confer increased susceptibility to breast and ovarian cancer. Its role in sporadic carcinogenesis is not well defined. Somatic mutations in breast cancers have not been reported and to date there are only three reports of somatic mutations in sporadic ovarian cancers. To investiga...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202847
更新日期:1999-08-12 00:00:00
abstract::Constitutively active AR-V7, one of the major androgen receptor (AR) splice variants lacking the ligand-binding domain, plays a key role in the development of castration-resistant prostate cancer (CRPC) and anti-androgen resistance. However, our understanding of the regulatory mechanisms of AR-V7-driven transcription ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-017-0047-5
更新日期:2018-03-01 00:00:00
abstract::Tumor suppressor p53 functions are downregulated in most cervical cancers, because the product of human papilloma virus (HPV) oncogene E6 binds to and inactivates p53 by promoting its degradation. p73, a p53 homologue, is similar to p53 in structure and function but yet not degraded by HPV E6 gene product. In this stu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206908
更新日期:2003-11-20 00:00:00
abstract::O6-methylguanine-DNA methyltransferase (MGMT) repairs the cytotoxic and mutagenic O6-alkylguanine produced by alkylating agents such as chemotherapeutic agents and mutagens. Recent studies have shown that in a subset of tumors, MGMT expression is inversely linked to hypermethylation of the CpG island in the promoter r...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207183
更新日期:2003-12-04 00:00:00
abstract::Myxoid and round-cell liposarcomas share the translocation t(12;16)(q13;p11) creating the TLS-CHOP fusion gene as a common genetic alteration. We previously reported several unique characteristics of genomic sequences around the breakpoints in the TLS and CHOP loci, and among them was the presence of consensus recogni...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203943
更新日期:2000-11-23 00:00:00
abstract::Increased glucose consumption is a hallmark of cancer cells. The increased consumption and subsequent metabolism of glucose during proliferation creates the need for a constant supply of NAD, a co-factor in glycolysis. Regeneration of the NAD required to support enhanced glycolysis has been attributed to the terminal ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2017.36
更新日期:2017-07-06 00:00:00
abstract::Graft versus host disease (GvHD), which is the primary complication of allogeneic bone marrow transplantation, can alter the intestinal barrier targeted by activated donor T-cells. Chemical inhibition of the stress protein HSP90 was demonstrated in vitro to inhibit T-cell activation and to modulate endoplasmic reticul...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.242
更新日期:2016-06-02 00:00:00
abstract::Mutant p53 proteins were shown to exert complex DNA-interactions in vitro, like binding to MAR-DNA, but so far it is unknown whether such interactions also occur in vivo. Therefore we analysed the binding of mutant (mut) p53 (Gly245-->Ser) in Onda 11 glioma cells to cellular DNA in vivo, using p53-specific chromatin i...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203745
更新日期:2000-08-24 00:00:00
abstract::The Rel/NF-kappaB transcription factors regulate the expression of many genes. The activity of RelA, a member of the Rel/NF-kappaB transcription factor family, is constitutively activated in the majority of pancreatic adenocarcinomas and cell lines. We report that the urokinase-type plasminogen activator (uPA), one of...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202833
更新日期:1999-08-12 00:00:00
abstract::The matricellular protein CCN5/WISP-2 represents a promising target in triple-negative breast cancer (TNBC) because treatment or induced activation of CCN5 in TNBC cells promotes cell growth arrest at the G0/G1 phase, reduces cell proliferation and delays tumor growth in the xenograft model. Our studies found that the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.250
更新日期:2015-06-11 00:00:00
abstract::Expression level of metastasis-associated protein 1 (MTA1) is closely related to tumor growth and metastasis in various cancers. Although increased expression level of MTA1 was observed in hepatocellular carcinoma (HCC), role of MTA1 complex containing histone deacetylase (HDAC) in hepatitis B virus (HBV)-associated h...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1211000
更新日期:2008-05-29 00:00:00
abstract::Loss of von Hippel Lindau (VHL) protein function is a key driver of VHL diseases, including sporadic and inherited clear cell renal cell carcinoma. Modulation of the proteostasis of VHL, especially missense point-mutated VHL, is a promising approach to augmenting VHL levels and function. VHL proteostasis is regulated ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.40
更新日期:2016-09-22 00:00:00
abstract::We show that expression of the microtubule depolymerizing kinesin KIF2C is induced by transformation of immortalized human bronchial epithelial cells (HBEC) by expression of K-Ras(G12V) and knockdown of p53. Further investigation demonstrates that this is due to the K-Ras/ERK1/2 MAPK pathway, as loss of p53 had little...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.486
更新日期:2014-11-20 00:00:00
abstract::Antimicrobial peptides, such as the cathelicidin LL-37/hCAP-18 and its mouse homolog cathelicidin-related antimicrobial peptide (CRAMP), are important effectors of the innate immune system with direct anti-bacterial activity. Cathelicidin is possibly involved in the regulation of tumor cell growth. The aim of this stu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.248
更新日期:2014-05-22 00:00:00
abstract::Genomic instability is a hallmark feature of cancer cells, and can be caused by defective DNA repair, for instance due to inactivation of BRCA2. Paradoxically, loss of Brca2 in mice results in embryonic lethality, whereas cancer cells can tolerate BRCA2 loss. This holds true for multiple DNA repair genes, and suggests...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0470-2
更新日期:2019-02-01 00:00:00
abstract::Mouse plasmacytomas induced by pristane oil alone, or in combination with Abelson murine leukemia virus (A-MuLV), regularly carry one of three alternative chromosomal translocations that juxtapose c-myc to immunoglobulin heavy- or light-chain loci. E mu-c-myc transgenic mice develop translocation-free plasmacytomas af...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Hexokinase-II (HK2) is a key enzyme involved in glycolysis, which is required for breast cancer progression. However, the underlying post-translational mechanisms of HK2 activity are poorly understood. Here, we showed that Proviral Insertion in Murine Lymphomas 2 (PIM2) directly bound to HK2 and phosphorylated HK2 on ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0386-x
更新日期:2018-11-01 00:00:00
abstract::A single heat shock factor (HSF), mediating the heat shock response, exists from yeast to Drosophila, whereas several related HSFs have been found in mammals. This raises the question of the specific or redundant functions of the different members of the HSF family and in particular of HSF1 and HSF2, which are both ub...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.171
更新日期:2010-07-22 00:00:00
abstract::Checkpoint protein Chk1 has been identified as an Hsp90 client. Treatment with 100 nM geldanamycin (GM) for 24 h markedly reduced the Chk1 amount in Jurkat and ML-1 leukemia cell lines. Because Chk1 plays a central role in G2 checkpoint, we added GM to G2-arrested Jurkat and HL-60 cells pretreated with 50 nM doxorubic...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210978
更新日期:2008-05-15 00:00:00
abstract::Low dose treatment with the DNA methylation inhibitor decitabine has been shown to be applicable for the management of certain types of cancer. However, its antitumor effect and mechanisms are context dependent and its activity has never been systematically studied in bladder cancer treatment. We used mouse models, cu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0799-1
更新日期:2019-07-01 00:00:00
abstract::Previous observations suggest expression of cyclooxygenase-2 to convey macrophage protection towards apoptotic cell death. We reasoned prostaglandin formation and in turn a cAMP increase as the underlying protective principle. Here we report that exposure of macrophages to lipopolysaccharide/interferon-gamma or lipoph...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201926
更新日期:1998-07-23 00:00:00
abstract::MicroRNAs (miRNAs) as modulators of gene expression have been described to display both tumor-promoting and tumor-suppressive functions. Although their role has been studied in different tumor types, little is known about how they regulate nuclear factor κB (NF-κB) signaling in breast cancer. Here, we performed an unb...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.571
更新日期:2012-09-13 00:00:00