Abstract:
AIM:To study the alterations of myocardial taurine transport function, taurine transporter (TAUT), and cysteine sulfinate decarboxylase (CSD) mRNA as well as effect of exogenous taurine in rats with isoproterenol (ISO)-induced cardiomegaly. METHODS:[3H]-Taurine uptake and release on myocardium were determined. Binding sites of [3H]-taurine for myocardial sarcolemma were measured. TAUT and CSD mRNA levels were assayed using competitive quantitative reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS:ISO group as compared with control group, myocardial taurine uptake markedly reduced, taurine release obviously increased; Bmax value of [3H]-taurine binding on cardiac sarcolemma reduced by 42% (P<0.05); TAUT and CSD mRNA levels decreased by 40% and 38% (P<0.05), respectively. ISO+taurine group as compared with ISO-treated group, the amounts of taurine uptake and TAUT mRNA returned to normal; taurine release reduced; Bmax increased by 92% (P<0.01), and CSD mRNA content augmented by 23% (P<0.05). There were no statistical differences of Kd values among the four groups (P>0.05). CONCLUSION:The data indicate that the failure to generate sufficient TAUT on myocardial sarcolemma may be the fundamental abnormality in ISO-induced cardiac injury. The mechanism by which administration of taurine considerably improves ISO-induced cardiac damage is probably to increase the expression of TAUT gene and recover taurine transport function.
journal_name
Acta Pharmacol Sinjournal_title
Acta pharmacologica Sinicaauthors
Shi YR,Bu DF,Qi YF,Gao L,Jiang HF,Pang YZ,Tang CS,Du JBsubject
Has Abstractpub_date
2002-10-01 00:00:00pages
910-8issue
10eissn
1671-4083issn
1745-7254journal_volume
23pub_type
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journal_title:Acta pharmacologica Sinica
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doi:
更新日期:2001-12-01 00:00:00
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journal_title:Acta pharmacologica Sinica
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更新日期:2004-08-01 00:00:00
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doi:
更新日期:2004-06-01 00:00:00
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doi:
更新日期:2002-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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doi:
更新日期:2001-08-01 00:00:00
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journal_title:Acta pharmacologica Sinica
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Acta pharmacologica Sinica
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00