Expression of cytosolic and plastid acetyl-coenzyme A carboxylase genes in young wheat plants.

Abstract:

:Expression of cytosolic and plastid acetyl-coenzyme A carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5' splice site for the first intron accumulates to its highest level in roots. Cytosolic ACCase mRNA also consists of two species, one of which is present at approximately a constant level, whereas the other accumulates to a high level in the lower sheath section. It is likely that different promoters are also responsible for the two forms of cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase mRNAs in the sheath section of the plant are similar. ACCase protein level is significantly lower in the leaf blades, in parallel with changes in the total ACCase mRNA level. Homoeologous ACCase genes show the same expression patterns and similar mRNA levels, suggesting that none of the genes was silenced or acquired new tissue specificity after polyploidization.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Podkowinski J,Jelenska J,Sirikhachornkit A,Zuther E,Haselkorn R,Gornicki P

doi

10.1104/pp.013169

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

763-72

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

131

pub_type

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