当前位置： SCI文献检索 > ACTA PHARMACOLOGICA SINICA期刊下所有文献 > Activation of c-Jun and suppression of phospho-p44/42 were involved in diphenylhydantoin-induced apoptosis of cultured rat cerebellar granule neurons.

Activation of c-Jun and suppression of phospho-p44/42 were involved in diphenylhydantoin-induced apoptosis of cultured rat cerebellar granule neurons.

Abstract：

AIM:To investigate possible intracellular signal molecules involved in diphenylhydantoin (DPH)-mediated apoptosis of cerebellar granule neurons (CGN) and explore possible molecular mechanisms of neurotoxicity of DPH. METHODS:Fluorescein diacetate (FDA) stain, hochest 33258 stain, and agar gel electrophoresis were used to test morphological and biological characters of primary CGN and cortical neurons (CN) in the presence or absence of 100 micromol/L DPH; Western blot and RT-PCR were employed to further investigate apoptotic/survival signal moleculars involved in the neuronal apoptotic signal transdution. RESULTS:DPH 100 micromol/L induced a typical apoptosis of CGN but had no toxicity on CN. Cerebellar granule neural apoptosis induced by 100 micromol/L DPH was significantly inhibited by pre-treatment with SB203580 (10 micromol/L) or CEP-11004 (1 micromol/L) for 1 h. DPH markedly upregulated the levels of phospho-c-Jun (active c-Jun), total c-Jun protein and c-jun mRNA in CGN. The levels of phospho-c-Jun dramatically elevated by DPH at 8 h were significantly inhibited by SB203580 (10 micromol/L) or CEP-11004 (1 micromol/L). Moreover, the activities of p44/42 (ERK1/ERK2), other members of MAP kinases and generally believed to be important survival effetors in CGN, were markedly suppressed. However, the activities of both JNK and p38 were little affected in the process of apoptosis of CGN induced by 100 micromol/L DPH. CONCLUSION:The selective toxicity of DPH on CGN is likely due to its ability to induce apoptosis of CGN, it is a process involved activation of c-Jun and suppression of the activity of p44/42.

journal_name

Acta Pharmacol Sin

journal_title

Acta pharmacologica Sinica

authors

Zhao LZ,Su XW,Huang YJ,Qiu PX,Yan GM

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

539-48

issue

6

eissn

1671-4083

issn

1745-7254

journal_volume

24

pub_type

杂志文章

相关文献

ACTA PHARMACOLOGICA SINICA文献大全
  • Hydrogen peroxide mobilizes Ca2+ through two distinct mechanisms in rat hepatocytes.

    abstract：AIM:Hydrogen peroxide (H2O2) is produced during liver transplantation. Ischemia/reperfusion induces oxidation and causes intracellular Ca2+ overload, which harms liver cells. Our goal was to determine the precise mechanisms of these processes. METHODS:Hepatocytes were extracted from rats. Intracellular Ca2+ concentrat...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2008.4

    authors： Sato H,Takeo T,Liu Q,Nakano K,Osanai T,Suga S,Wakui M,Wu J

    更新日期：2009-01-01 00:00:00

  • Comparisons of somatic action potentials from dispersed and intact rat nodose ganglia using patch-clamp technique.

    abstract：AIM:To differentiate the electrophysiological characteristics of somatic action potentials (AP) from isolated Neo and Juv nodose sensory neurons (NSN) and those from slices of intact Juv and adult rat nodose ganglia. METHODS:For isolated cell recordings nodose ganglia from 3-8 d old Neo and 4 weeks old Juv rats were d...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Li BY,Schild JH

    更新日期：2002-06-01 00:00:00

  • Isolation of acetylcholinesterase G4 and G1 molecular isoforms from rat cortex.

    abstract：AIM:To isolate the G4 and G1 molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs. METHODS:Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and G1 molecular forms of AChE. RESULTS:After size exclusion...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Zhao Q,Tang XC

    更新日期：2002-02-01 00:00:00

  • Ca2+ signaling during vertebrate somitogenesis.

    abstract：:A variety of Ca2+ signals, in the form of intercellular pulses and waves, have been reported to be associated with the various sequential stages of somitogenesis: from convergent extension and the formation of the paraxial mesoderm; during the patterning of the paraxial mesoderm to establish segmental units; throughou...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7254.2006.00401.x

    authors： Webb SE,Miller AL

    更新日期：2006-07-01 00:00:00

  • Aspirin alleviates cardiac fibrosis in mice by inhibiting autophagy.

    abstract：:Aspirin (ASA) is a cardioprotective drug with anti-cardiac fibrosis action in vivo. This study was aimed to clarify the anti-cardiac fibrosis action of ASA and the underlying mechanisms. Two heart injury models (injection of isoproterenol and ligation of the left anterior descending branch) were used in mice to induce...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2016.143

    authors： Liu PP,Liu HH,Sun SH,Shi XX,Yang WC,Su GH,Zhao J

    更新日期：2017-04-01 00:00:00

  • Novel neuroprotectant chiral 3-n-butylphthalide inhibits tandem-pore-domain potassium channel TREK-1.

    abstract：AIM:To study the effects of 3-n-butylphthalide (NBP) on the TREK-1 channel expressed in Chinese hamster ovary (CHO) cells. METHODS:Whole-cell patch-clamp recording was used to record TREK-1 channel currents. The effects of varying doses of l-NBP on TREK-1 currents were also observed. Current-clamp recordings were perf...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2010.210

    authors： Ji XC,Zhao WH,Cao DX,Shi QQ,Wang XL

    更新日期：2011-02-01 00:00:00

  • Sodium butyrate and dexamethasone promote exocrine pancreatic gene expression in mouse embryonic stem cells.

    abstract：AIM:The feasibility of inducing endocrine pancreatic differentiation of embryonic stem (ES) cells has been well documented. However, whether ES cells possess the potential for exocrine pancreatic differentiation requires further exploration. Here, we investigated whether sodium butyrate and glucocorticoids were conduci...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2009.115

    authors： Ren M,Yan L,Shang CZ,Cao J,Li FP,Li JY,Cheng H,Min J

    更新日期：2009-09-01 00:00:00

  • Functions and the related signaling pathways of the neurotrophic factor neuritin.

    abstract：:Neuritin is a member of the neurotrophic factor family, which is activated by neural activity and neurotrophins, and promotes neurite growth and branching. It has shown to play an important role in neuronal plasticity and regeneration. It is also involved in other biological processes such as angiogenesis, tumorigenes...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2017.197

    authors： Yao JJ,Zhao QR,Lu JM,Mei YA

    更新日期：2018-09-01 00:00:00

  • A novel synthetic compound MCAP suppresses LPS-induced murine microglial activation in vitro via inhibiting NF-kB and p38 MAPK pathways.

    abstract：AIM:To investigate the anti-neuroinflammatory activity of a novel synthetic compound, 7-methylchroman-2-carboxylic acid N-(2-trifluoromethyl) phenylamide (MCAP) against LPS-induced microglial activation in vitro. METHODS:Primary mouse microglia and BV2 microglia cells were exposed to LPS (50 or 100 ng/mL). The express...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2015.138

    authors： Kim BW,More SV,Yun YS,Ko HM,Kwak JH,Lee H,Suk K,Kim IS,Choi DK

    更新日期：2016-03-01 00:00:00

  • Cycloastragenol upregulates SIRT1 expression, attenuates apoptosis and suppresses neuroinflammation after brain ischemia.

    abstract：:Cycloastragenol (CAG) is the active form of astragaloside IV isolated from Astragalus Radix, which displays multiple pharmacological effects. Silent information regulator 1 (SIRT1), a class III histone deacetylase, has been shown to play an important role in neuroprotection against cerebral ischemia. In this study, we...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-0386-6

    authors： Li M,Li SC,Dou BK,Zou YX,Han HZ,Liu DX,Ke ZJ,Wang ZF

    更新日期：2020-08-01 00:00:00

  • Adenovirus-mediated expression of Tob1 sensitizes breast cancer cells to ionizing radiation.

    abstract：AIM:To investigate the effect of the Tob1 gene, a member of the Transducing Molecule of ErbB2/B-cell Translocation Ggene (TOB/BTG) family, by using the adenovirus-mediated expression of Tob1 on radiosensitivity in a human breast cancer cell line MDA-MB-231. METHODS:Cell survival was determined by clonogenic assay. Apo...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00647.x

    authors： Jiao Y,Ge CM,Meng QH,Cao JP,Tong J,Fan SJ

    更新日期：2007-10-01 00:00:00

  • Smart nanoparticles improve therapy for drug-resistant tumors by overcoming pathophysiological barriers.

    abstract：:The therapeutic outcome of chemotherapy is severely limited by intrinsic or acquired drug resistance, the most common causes of chemotherapy failure. In the past few decades, advancements in nanotechnology have provided alternative strategies for combating tumor drug resistance. Drug-loaded nanoparticles (NPs) have se...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2016.84

    authors： Liu JP,Wang TT,Wang DG,Dong AJ,Li YP,Yu HJ

    更新日期：2017-01-01 00:00:00

  • Crocetin induces cytotoxicity and enhances vincristine-induced cancer cell death via p53-dependent and -independent mechanisms.

    abstract：AIM:To investigate the anticancer effect of crocetin, a major ingredient in saffron, and its underlying mechanisms. METHODS:Cervical cancer cell line HeLa, non-small cell lung cancer cell line A549 and ovarian cancer cell line SKOV3 were treated with crocetin alone or in combination with vincristine. Cell proliferatio...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.109

    authors： Zhong YJ,Shi F,Zheng XL,Wang Q,Yang L,Sun H,He F,Zhang L,Lin Y,Qin Y,Liao LC,Wang X

    更新日期：2011-12-01 00:00:00

  • Icariside II attenuates cerebral ischemia/reperfusion-induced blood-brain barrier dysfunction in rats via regulating the balance of MMP9/TIMP1.

    abstract：:Cerebral ischemia/reperfusion (I/R) results in harmful consequences during ischemic stroke, especially the disruption of the blood-brain barrier (BBB), which leads to severe hemorrhagic transformation through aggravation of edema and brain hemorrhage. Our previous study demonstrated that icariside II (ICS II), which i...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-0409-3

    authors： Liu MB,Wang W,Gao JM,Li F,Shi JS,Gong QH

    更新日期：2020-12-01 00:00:00

  • Effects of angiotensin II on release of CRH and AVP from hypothalamus during acute hypoxia.

    abstract：AIM:To investigate the effects of angiotensin II (Ang II) on corticotropin-releasing hormone (CRH) and argipressin (AVP) release from median eminence (ME) of hypothalamus during acute hypoxia in rats. METHODS:Simulated hypoxia was performed in a hypobaric chamber. CRH and AVP were determined by radioimmunoassay (RIA)....

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Wu Y,Du JZ

    更新日期：2000-11-01 00:00:00

  • Anti-PD-L1 mediating tumor-targeted codelivery of liposomal irinotecan/JQ1 for chemo-immunotherapy.

    abstract：:Immune checkpoint blockade therapy has become a first-line treatment in various cancers. But there are only a small percent of colorectal patients responding to PD-1/PD-L1 blockage immunotherapy. How to increase their treatment efficacy is an urgent and clinically unmet need. It is acknowledged that immunogenic cell d...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-00570-8

    authors： He ZD,Zhang M,Wang YH,He Y,Wang HR,Chen BF,Tu B,Zhu SQ,Huang YZ

    更新日期：2020-12-11 00:00:00

  • Why should autophagic flux be assessed?

    abstract：:As autophagy is involved in cell growth, survival, development and death, impaired autophagic flux has been linked to a variety of human pathophysiological processes, including neurodegeneration, cancer, myopathy, cardiovascular and immune-mediated disorders. There is a growing need to identify and quantify the status...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2012.184

    authors： Zhang XJ,Chen S,Huang KX,Le WD

    更新日期：2013-05-01 00:00:00

  • Growth inhibitory effects and molecular mechanisms of crotoxin treatment in esophageal Eca-109 cells and transplanted tumors in nude mice.

    abstract：AIM:To investigate the antitumor actions of the Crotalus durissus neurotoxin (crotoxin) on human esophageal carcinoma (Eca-109) cells in vitro and transplanted esophageal Eca-109 tumors in nude mice. METHODS:The growth-inhibitory effect was analyzed in Eca-109 cells using MTT assay. Cell morphology changes in nuclei w...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2012.156

    authors： He JK,Wu XS,Wang Y,Han R,Qin ZH,Xie Y

    更新日期：2013-02-01 00:00:00

  • Effects of melittin on isolated papillary muscles of guinea pig.

    abstract：AIM:To investigate the effect of melittin (Mel) on papillary muscles of guinea pigs. METHODS:Contraction of papillary muscles were examined by conventional method and action potentials (AP) were recorded by standard glass microelectrode technique. RESULTS:Mel (0.5, 3 micromol/L) significantly increased the contractil...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Zhang YL,Yang S,Shi NC,Jiang MH

    更新日期：2001-08-01 00:00:00

  • Organ preservation: from the past to the future.

    abstract：:Organ transplantation is the most effective therapy for patients with end-stage disease. Preservation solutions and techniques are crucial for donor organ quality, which is directly related to morbidity and survival after transplantation. Currently, static cold storage (SCS) is the standard method for organ preservati...

    journal_title：Acta pharmacologica Sinica

    pub_type： 历史文章,杂志文章,评审

    doi：10.1038/aps.2017.182

    authors： Jing L,Yao L,Zhao M,Peng LP,Liu M

    更新日期：2018-05-01 00:00:00

  • Bioavailability, tissue distribution, and excretion characteristics of the novel carbonic anhydrase inhibitor tolsultazolamide in rats.

    abstract：AIM:Tolsultazolamide, a novel carbonic anhydrase inhibitor, is designed for the prophylaxis and treatment of acute mountain sickness. The aim of this study was to investigate the pharmacokinetics, tissue distribution, and excretion characteristics of tolsultazolamide and the sex difference in pharmacokinetics in rats. ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2013.146

    authors： Wang JD,Shi YP,Yin J,Pan ZY,Cui WY,Zhang YF,Wang H

    更新日期：2014-02-01 00:00:00

  • Hypoxia, HIF-1α, and COVID-19: from pathogenic factors to potential therapeutic targets.

    abstract：:The pandemic of coronavirus disease 2019 (COVID-19) and its pathogen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have become the greatest current threat to global public health. The highly infectious SARS-CoV-2 virus primarily attacks pulmonary tissues and impairs gas exchange leading to acute respir...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/s41401-020-00554-8

    authors： Serebrovska ZO,Chong EY,Serebrovska TV,Tumanovska LV,Xi L

    更新日期：2020-12-01 00:00:00

  • Association of CYP3A polymorphisms with the pharmacokinetics of cyclosporine A in early post-renal transplant recipients in China.

    abstract：AIM:To evaluate retrospectively the association of cytochrome P450 3A (CYP3A) and ATP-binding cassette sub-family B member 1 (ABCB1) gene polymorphisms with the pharmacokinetics of cyclosporine A (CsA) in Chinese renal transplant patients. METHODS:One hundred and twenty-six renal transplant patients were recruited. Bl...

    journal_title：Acta pharmacologica Sinica

    pub_type： 临床试验,杂志文章

    doi：10.1038/aps.2012.136

    authors： Meng XG,Guo CX,Feng GQ,Zhao YC,Zhou BT,Han JL,Chen X,Shi Y,Shi HY,Yin JY,Peng XD,Pei Q,Zhang W,Wang G,He M,Liu M,Yang JK,Zhou HH

    更新日期：2012-12-01 00:00:00

  • Pathogenic roles of microvesicles in diabetic retinopathy.

    abstract：:Diabetic retinopathy (DR) is a common complication of diabetes and has been recognized as the leading cause of blindness in adults. Several interrelated molecular pathways are involved in the development of DR. Microvesicles (MVs) are cell membrane vesicles, which carry many biologic molecules, such as mRNAs, microRNA...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2017.77

    authors： Zhang W,Chen S,Liu ML

    更新日期：2018-01-01 00:00:00

  • Protein tyrosine phosphatases as potential therapeutic targets.

    abstract：:Protein tyrosine phosphorylation is a key regulatory process in virtually all aspects of cellular functions. Dysregulation of protein tyrosine phosphorylation is a major cause of human diseases, such as cancers, diabetes, autoimmune disorders, and neurological diseases. Indeed, protein tyrosine phosphorylation-mediate...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2014.80

    authors： He RJ,Yu ZH,Zhang RY,Zhang ZY

    更新日期：2014-10-01 00:00:00

  • Insights into the molecular mechanisms of Polygonum multiflorum Thunb-induced liver injury: a computational systems toxicology approach.

    abstract：:An increasing number of cases of herb-induced liver injury (HILI) have been reported, presenting new clinical challenges. In this study, taking Polygonum multiflorum Thunb (PmT) as an example, we proposed a computational systems toxicology approach to explore the molecular mechanisms of HILI. First, the chemical compo...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2016.147

    authors： Wang YY,Li J,Wu ZR,Zhang B,Yang HB,Wang Q,Cai YC,Liu GX,Li WH,Tang Y

    更新日期：2017-05-01 00:00:00

  • Open channel block of Kv1.5 currents by citalopram.

    abstract：AIM:To examine whether selective serotonin reuptake inhibitor citalopram interacts with Kv1.5, one of the cardiovascular-specific Kv channel isoforms. METHODS:The interaction between citalopram and Kv1.5 expressed in Chinese hamster ovary cells was studied using the whole-cell patch-clamp technique. RESULTS:Citalopra...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2010.14

    authors： Lee HM,Hahn SJ,Choi BH

    更新日期：2010-04-01 00:00:00

  • Gambogic acid induces death inducer-obliterator 1-mediated apoptosis in Jurkat T cells.

    abstract：AIM:To explore the anticancer effects and the molecular mechanisms of gambogic acid (GA) on Jurkat cells. METHODS:Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Annexin V-fluorescein-isothiocyanate/propidium iodide, DNA defragmentation, and comet assay were used to d...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2008.00762.x

    authors： Wang Y,Chen Y,Chen Z,Wu Q,Ke WJ,Wu QL

    更新日期：2008-03-01 00:00:00

  • Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer.

    abstract：:Korean Panax ginseng C A Meyer is mainly used to maintain the homeostasis of the body, and the pharmacological efficacy of Korean ginseng identified by modern science includes improved brain function, pain-relieving effects, preventive effects against tumors as well as anti-tumor activity, enhanced immune system funct...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7254.2008.00869.x

    authors： Choi KT

    更新日期：2008-09-01 00:00:00

  • Changes and mechanisms of protein-tyrosine kinase and protein-tyrosine phosphatase activities after brain ischemia/reperfusion.

    abstract：AIM:To study the changes and mechanisms of protein-tyrosine kinase (PTK) and protein-tyrosine phosphatase (PTP) activities in the hippocamal synaptosome following cerebral ischemia/reperfusion (I/R) in gerbil. METHODS:Transient (15 min) global ischemia was produced by bilateral carotid artery occlusion. Total PTK and ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Pei L,Li Y,Yan JZ,Zhang GY,Cui ZC,Zhu ZM

    更新日期：2000-08-01 00:00:00