Abstract:
:Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a critical role in growth and differentiation of myeloid cells. We previously reconstituted high affinity human GM-CSF receptor (hGM-CSFR) in a proB cell line BA/F3 by cotransfecting alpha and beta chain cDNA clones and showed that the reconstituted receptor could transduce growth promoting signals. The high affinity hGM-CSFR was also reconstituted in mouse NIH3T3 cells, but its ability to transduce signals in fibroblasts remained unanswered. In the present study, we further characterized signal transduction by the reconstituted hGM-CSFR both in NIH3T3 cells and BA/F3 cells. We found that the reconstituted hGM-CSFR transduces signals in NIH3T3 fibroblasts and BA/F3 cells in response to human GM-CSF to activate transcription of c-fos, c-jun and c-myc protooncogenes. hGM-CSF also induces protein tyrosine phosphorylation and DNA synthesis in both cell types. The ability of hGM-CSFR to transduce signals was affected by inhibitors of tyrosine kinase. These results indicated that the hGM-CSFR is functional in fibroblasts, that signal transduction via the hGM-CSFR in fibroblasts involves tyrosine kinase(s) and that association of hGM-CSFR with factor(s) specific to hematopoietic cell lineage is not essential to transduce growth promoting signals.
journal_name
Leukemiajournal_title
Leukemiaauthors
Yokota T,Watanabe S,Mui AL,Muto A,Miyajima A,Arai Ksubject
Has Abstractpub_date
1993-08-01 00:00:00pages
S102-7eissn
0887-6924issn
1476-5551journal_volume
7 Suppl 2pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract::Activation of the MEK/ERK/MAP kinase signaling pathway promotes the proliferation and survival of hematopoietic cells. The kinases MEK-1, MEK-2, ERK-1/MAPK and ERK-2/MAPK are activated by phosphorylation at specific sites, and these events can be monitored using phospho-specific antibodies. In this report we examined ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402400
更新日期:2002-04-01 00:00:00
abstract::In this study, the long-term outcome of 1818 patients treated in five consecutive clinical trials (the cooperative study group for childhood acute lymphoblastic leukaemia (COALL) 82, 85, 89, 92 and 97) from 24 cooperating centres in Germany is reported. The probability of event-free survival (pEFS) improved significan...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:10.1038/leu.2009.249
更新日期:2010-02-01 00:00:00
abstract::Acute graft-versus-host disease (aGVHD) is a common complication of allogeneic hematopoietic stem cell transplantation (alloHCT) and is a major cause of morbidity and mortality. Systemic steroid therapy is the first-line treatment for aGVHD, although about half of patients will become refractory to treatment. As the n...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/s41375-020-0804-2
更新日期:2020-05-01 00:00:00
abstract::During infection with different retroviruses, high levels of unintegrated extrachromosomal DNA accumulate in infected cells. While extrachromosomal linear DNA is the immediate precursor of the integrated provirus, the function, if any, of extrachromosomal circular DNA has been unclear. Several groups have attempted to...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2400776
更新日期:1997-09-01 00:00:00
abstract::Adults with acute lymphoblastic leukemia (ALL) do worse than children. From 7/2008 to 12/2014, Nordic and Baltic centers treated 1509 consecutive patients aged 1-45 years with Philadelphia chromosome-negative ALL according to the NOPHO ALL2008 without cranial irradiation. Overall, 1022 patients were of age 1-9 years (...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.265
更新日期:2018-03-01 00:00:00
abstract::Allogeneic and autologous stem cell transplantation (SCT) are increasingly considered for treatment of patients with chronic lymphocytic leukemia (CLL). In order to assess the potential therapeutic value of SCT for CLL, the present article aims at answering the following crucial questions: (1) Is SCT a curative treatm...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2402530
更新日期:2002-06-01 00:00:00
abstract::Immunoglobulin (IG) gene repertoire restrictions strongly support antigen selection in the pathogenesis of chronic lymphocytic leukemia (CLL). Given the emerging multifarious interactions between CLL and bystander T cells, we sought to determine whether antigen(s) are also selecting T cells in CLL. We performed a larg...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2016.362
更新日期:2017-07-01 00:00:00
abstract::Since hemopoietic cells are refractory to transfection by conventional chemical means, we have developed a reliable and efficient gene transfer system for K562 cells which uses electric field-mediated gene transfer (EFMGT). EFMGT involves the exposure of cells in suspension to an electric field which transiently allow...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Basic fibroblast growth factor (bFGF) is a pleiotropic cytokine which has recently been shown to delay fludarabine-induced apoptosis in B cell chronic lymphocytic leukemia (B-CLL) cells. To investigate the potential mechanism of bFGF-mediated delay of apoptosis, two EBV-transformed B prolymphocytic cell lines (JVM-2, ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2400556
更新日期:1997-02-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Leukemia
pub_type: 已发布勘误
doi:10.1038/s41375-020-0782-4
更新日期:2020-09-01 00:00:00
abstract::Treatment resistance in T-cell acute lymphoblastic leukemia (T-ALL) is associated with phosphatase and tensin homolog (PTEN) deletions and resultant phosphatidylinositol 3'-kinase (PI3K)-AKT pathway activation, as well as MYC overexpression, and these pathways repress mitochondrial apoptosis in established T-lymphobla...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2014.78
更新日期:2014-09-01 00:00:00
abstract::MK-0457, an Aurora kinase and BCR-ABL inhibitor, was studied on a Phase I/II study in 77 patients with refractory hematologic malignancies. The average number of cycles per patient was 3 (range 1-21). Maximum tolerated doses for a 5-day short infusion and continuous infusion regimens were 40 mg/m(2)/h and 144 mg/m(2)/...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2012.186
更新日期:2013-01-01 00:00:00
abstract::Polymerase chain reaction (PCR) techniques based on amplification and identification of leukemia-specific DNA sequences provide a sensitive diagnostic method for detection of minimal residual disease (MRD) with a detection limit of 10(-5) to 10(-6) (1-10 malignant cells in 10(6) normal cells). To date, the main leukem...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::In this study, the abilities of constitutive and conditional forms of the three Raf kinases to abrogate the cytokine dependency of FDC-P1 cells were examined. The constitutively active forms (delta) of all three Raf kinases were fused to the hormone-binding domain of the estrogen receptor (ER), rendering their activit...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401720
更新日期:2000-04-01 00:00:00
abstract::Abnormal karyotypes are common in cancer cells and frequently observed in acute myeloid leukemia (AML), in which complex karyotype aberrations are associated with poor prognosis. How exactly abnormal karyotypes arise and are propagated in AML is unclear. TP53 mutations and deletions are frequent in complex karyotype A...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/s41375-019-0550-5
更新日期:2019-11-01 00:00:00
abstract::In a study carried out for patients receiving intrafamilial HLA-A,B,DR identical, MLC negative bone marrow transplants, RFLP profiles of HLA-class II for 27 donor recipient pairs were analyzed. Twenty-four pairs were found HLA-class II identical while three pairs were HLA-DP incompatible. The patients of these three p...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Clinical outcomes for multiple myeloma (MM) are highly heterogeneous and it is now clear that pivotal genetic events are the primary harbingers of such variation. These findings have broad implications for counseling, choice of therapy and the design and interpretation of clinical investigation. Indeed, as in acute le...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404516
更新日期:2007-03-01 00:00:00
abstract::The AML1/ETO fusion transcript can be detected by reverse transcription polymerase chain reaction (RT-PCR) in patients with t(8;21)-associated acute myeloid leukemia (AML) in long-term complete remission (CR). Quantitation of the amount of the fusion transcript during CR may therefore be more predictive of cure or rel...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401128
更新日期:1998-09-01 00:00:00
abstract::Resistance to anthracyclines is related to a poor prognosis in childhood acute lymphoblastic leukemia (ALL). Resistance to this class of drugs may (partly) be reversed by modulating agents, as has been demonstrated in a variety of cell lines. However, it is unknown which modulators may be of clinical benefit in childh...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401035
更新日期:1998-06-01 00:00:00
abstract::Fresh and/or frozen bone marrow cells from five healthy individuals and seven patients with myeloid leukemia were studied using growth factors and a cytogenetic technique which allows simultaneous analysis of karotype and cell lineage. Cell lineages were identified using monoclonal antibodies in an alkaline phosphatas...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::The interactions between haemopoietic progenitor cells and marrow stromal cells that are essential for the regulation of normal haemopoiesis are defective in chronic phase chronic myeloid leukaemia (CML). The presence of primitive progenitor cells (blast colony-forming cells, Bl-CFC) in the blood of patients with CML ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::ET is a chronic myeloproliferative disorder rarely evolving into AML, sometimes preceded by a myelodysplastic syndrome (MDS). Such transformations mostly occur in patients treated with radiophosphorous ((32)P) or alkylating agents, especially busulfan. Recently, concern has also arisen about the long-term safety of hy...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402638
更新日期:2002-10-01 00:00:00
abstract::Successful treatment results for MLL-rearranged Acute Lymphoblastic Leukemia (ALL) in infants remain difficult to achieve. Significantly contributing to therapy failure is poor response to glucocorticoids (GCs), like prednisone. Thus, overcoming resistance to these drugs may be a crucial step towards improving prognos...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2013.245
更新日期:2014-04-01 00:00:00
abstract::HOX homeobox proteins are key oncogenic drivers in hematopoietic malignancies. Here we demonstrate that HOXA1, HOXA6 and predominantly HOXA9 are able to induce the production of insulin-like growth factor 1 (Igf1). In chromatin immunoprecipitations, HOXA9 bound directly to the putative promoter and a DNase-hypersensit...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2014.287
更新日期:2015-04-01 00:00:00
abstract::Multidrug resistance (MDR), a significant impediment to the successful treatment of cancer clinically, has been attributed to the overexpression of P-glycoprotein (P-gp), a plasma membrane multidrug efflux transporter. P-gp maintains sublethal intracellular drug concentrations by virtue of its drug efflux capacity. Th...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2009.76
更新日期:2009-09-01 00:00:00
abstract::In this work we have mapped by double-label immunofluorescence the cellular distribution of integrins and their relationship with cytoskeletal proteins in normal and malignant monocytes. In normal monocytes, CD18 and CD11c are concentrated at specific adhesion sites, named podosomes, together with actin, vinculin, and...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::The chimeric fusion oncogene early B-cell factor 1-platelet-derived growth factor receptor-β (EBF1-PDGFRB) is a recurrent lesion observed in Philadelphia-like B-acute lymphoblastic leukemia (B-ALL) and is associated with particularly poor prognosis. While it is understood that this fusion activates tyrosine kinase sig...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.166
更新日期:2018-01-01 00:00:00
abstract::Deletion and translocation involving the bands 11q14 and 11q21 have been detected in five patients with T cell acute lymphoblastic leukemia (ALL). The breakpoint on chromosome 11 was on the same band as that previously described in some acute nonlymphocytic leukemias: monocytic or myelomocytic. The existence of a new ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::Mantle cell lymphoma (MCL) is a moderately aggressive B-cell lymphoma that responds poorly to currently used therapeutic protocols. In order to identify tumour characteristics that improve the understanding of biology of MCL, analysis of oligonucleotide microarrays were used to define specific gene expression profiles...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403057
更新日期:2003-09-01 00:00:00
abstract::Cytogenetic evolution in the myelodysplastic syndrome (MDS) has been associated with an abrupt shift to acute myelogenous leukemia (AML). To investigate the 'evolution' of MDS to AML we compared the karyotypes of MDS patients at presentation and at development of AML. Of 170 patients with MDS who developed AML, 63 had...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00