Abstract：
:Since hemopoietic cells are refractory to transfection by conventional chemical means, we have developed a reliable and efficient gene transfer system for K562 cells which uses electric field-mediated gene transfer (EFMGT). EFMGT involves the exposure of cells in suspension to an electric field which transiently allows the entry of DNA into the cell and its subsequent integration and expression. Plasmids bearing the neo gene were used to identify and select transfected clonogenic cells manifested by geneticin resistance in semisolid medium. Transfection efficiency is significantly affected by the following variables: voltage, capacitance, time constant, number of pulses, buffer type and temperature, DNA concentration, configuration, and promoter type. Cell cycle status also appears to be critical as shown in studies employing aphidicolin synchronization. Using optimal conditions, we have consistently achieved a transfection efficiency of 0.3-0.4% of clonogenic cells per microgram DNA. Stability of neo gene expression was also demonstrated after 4 months in nonselective culture conditions. This level of efficiency compares favorably with other reports of gene transfer into human hemopoietic progenitor cells.
journal_name
Leukemiajournal_title
Leukemiaauthors
Croaker GM,Wass EJ,Iland HJsubject
Has Abstractpub_date
1990-07-01 00:00:00pages
502-7issue
7eissn
0887-6924issn
1476-5551journal_volume
4pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract：:Nasal-type natural killer (NK) cell lymphoma is an infrequent aggressive malignant disease with very poor prognosis. We aimed to explore the possible role of the transcription factor STAT3 in the pathophysiology of this malignancy, as it was involved in oncogenesis and chemoresistance. For this, we established and cha...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2009.91
更新日期：2009-09-01 00:00:00
abstract：:A series of 60 acute nonlymphocytic leukemias (ANLL) was analyzed for the expression of terminal deoxynucleotidyl transferase (TdT). The detected TdT+ cells were studied in detail by use of double marker analyses for TdT and differentiation markers, such as myeloid markers (CD13 and CD33), B cell markers, T cell marke...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:Outcomes for patients with chronic myeloid leukemia (CML) have substantially improved due to advances in drug development and rational treatment intervention strategies. Despite these significant advances there are still unanswered questions on patient management regarding how to more reliably predict treatment failur...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：10.1038/s41375-019-0512-y
更新日期：2019-08-01 00:00:00
abstract：:We previously demonstrated that the EVI-1 gene was transcriptionally activated in the 3q21q26 syndrome and chromosomal breakpoints at 3q26 were clustered within 400 Kb of the EVI-1 gene. Since thrombocytosis is often observed in the 3q21q26 syndrome, we first mapped the thrombopoietin (TPO) gene and then we examined f...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:Population-based studies of classical Hodgkin lymphoma (cHL) in contemporary clinical practice are scarce. The aim of this nationwide population-based study is to assess trends in primary therapy and relative survival (RS) during 1989-2017. We included 9,985 patients with cHL. Radiotherapy alone was virtually not appl...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/s41375-020-0875-0
更新日期：2020-05-28 00:00:00
abstract：:The T-lineage phenotype in children with acute lymphoblastic leukaemia (ALL) is associated with in vitro drug resistance and a higher relapse-risk compared to a precursor B phenotype. Our study was aimed to investigate whether mutations in the ATM gene occur in childhood T-lineage acute lymphoblastic leukaemia (T-ALL)...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2403943
更新日期：2005-11-01 00:00:00
abstract：:The expression of the myeloperoxidase (MPO) gene was studied, by means of Northern blot analysis in 14 cases of acute myeloid leukemia (AML), 11 cases of chronic myeloid leukemia (CML), and 6 cases of CML blast crisis, and in HL60 cells before and after induction of terminal differentiation with retinoic acid (RA), ph...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：:Chimeric antigen receptor (CAR)-T-cell is a safe and effective therapy of B-cell cancers but it is unknown if this is so in persons with prior hepatitis B virus (HBV) infection. We studied 70 subjects with advanced B-cell cancers receiving CAR-T-cell therapy, 12 of whom had chronic HBV-infection (HBsAg positive) and 2...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/s41375-020-0936-4
更新日期：2020-10-01 00:00:00
abstract：:Less-intense remission induction regimens for adults with newly diagnosed acute myeloid leukemia (AML) aim to reduce treatment-related mortality (TRM), here defined as death within 4 weeks after starting induction therapy. This assumes that TRM rates are similar to the 15-20% observed 20 years ago. Herein we test this...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2013.176
更新日期：2014-02-01 00:00:00
abstract：:Primary myelofibrosis (PMF) is a hematopoietic stem cell (HSC) disease, characterized by aberrant differentiation of all myeloid lineages and profound disruption of the bone marrow niche. PMF samples carry several mutations, but their cell origin and hierarchy in regulating the different waves of clonal and aberrant m...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/s41375-018-0159-0
更新日期：2019-01-01 00:00:00
abstract：:Twenty new cases of acute lymphoblastic leukemia (ALL) with the dicentric chromosome dic(9;20)(p1113;q11) are presented. This chromosomal abnormality is difficult to identify from G-banding alone. It masquerades as monosomy 20 and is only accurately identified by fluorescence in situ hybridization (FISH). Monosomy 20 ...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2401654
更新日期：2000-02-01 00:00:00
abstract：:The high efficacy of the standard treatment of chronic myeloid leukemia (CML) with imatinib has prompted the need for accurate methods to monitor response at levels below the landmark of complete cytogenetic remission. Quantification of BCR-ABL transcripts has proven to be the most sensitive method available, and has ...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：10.1038/leu.2009.168
更新日期：2009-11-01 00:00:00
abstract：:Real-time quantitative polymerase chain reaction (PCR) for BCR-ABL mRNA in the peripheral blood (RQ-PCR) provides an accurate and reliable measure of response to therapy in chronic myeloid leukaemia (CML). We wanted to determine in what circumstances additional clinically relevant information was provided by simultane...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2404139
更新日期：2006-04-01 00:00:00
abstract：:Bone marrow cells from most patients with acute promyelocytic leukemia contain a highly specific cytogenetic rearrangement, a reciprocal translocation between the long arms of chromosomes 15 and 17. Several cases of variant translocations involving 17q but not 15q have been reported, leading to the suggestion that the...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:A human acute lymphoblastic leukemia (ALL) cell line, BALM-9, was established from the peripheral blood specimen of a immunoglobin (lg) phenotype, the established BALM-9 cell line expressed both kappa and lambda light (L) chains simultaneously in a range of 30-80%. Two-color flow cytometric analysis demonstrated that ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among...
journal_title：Leukemia
pub_type： 杂志文章,评审
doi：10.1038/leu.2012.122
更新日期：2012-09-01 00:00:00
abstract：:Recent studies suggest that most cases of myelodysplastic syndrome (MDS) are clonally heterogeneous, with a founding clone and multiple subclones. It is not known whether specific gene mutations typically occur in founding clones or subclones. We screened a panel of 94 candidate genes in a cohort of 157 patients with ...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2013.58
更新日期：2013-06-01 00:00:00
abstract：:By combining the polymerase chain reaction and differential library screening, a cDNA for an mRNA expressed in chicken avian myeloblastosis virus (AMV)-transformed monoblasts was isolated. This mRNA is not expressed in erythroblast or T-lymphoblast cell lines. Induced differentiation of the cells of the AMV-transforme...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Hematopoiesis is orchestrated by interactions between hematopoietic stem/progenitor cells (HSPCs) and stromal cells within bone marrow (BM) niches. Side population (SP) functionality is a major characteristic of HSPCs related to quiescence and resistance to drugs and environmental stresses. At steady state, SP cells a...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/leu.2013.256
更新日期：2014-04-01 00:00:00
abstract：:An increasing number of variants of unknown significance are being identified in leukemia patients with the application of deep sequencing and these include CSF3R cytoplasmic mutations. Previous studies have demonstrated oncogenic potential of certain CSF3R truncation mutations prior to internalization motifs. However...
journal_title：Leukemia
pub_type： 杂志文章,多中心研究
doi：10.1038/leu.2017.126
更新日期：2017-12-01 00:00:00
abstract：:We have examined the respiratory status of murine and human marrow progenitor cells proliferating in response to growth factors. Proliferation of fresh marrow cells, which are almost entirely entering terminal differentiation, depends on oxidative phosphorylation as determined by oxygen uptake, lactic acid production,...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Of 240 Japanese children with acute lymphoblastic leukemia (ALL) treated between 1983 and 1990, 75 (31%) had normal diploidy, 47 (20%) hyperdiploidy with more than 50 chromosomes, 18 (8%) hyperdiploidy with 47-50 chromosomes, 77 (32%) pseudodiploidy, 22 (9%) hypodiploidy and one hypotetraploidy in the leukemic cells. ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Schedule dependency of idarubicin (Ida) and doxorubicin (Dox) toxicity was investigated in vitro using the K562 human leukemia cell line. For Dox, repeated exposure to the IC30 (d x 3) resulted in comparable survival as single exposure to the total accumulative dose (20%). For Ida, repeated exposure to the IC30 (d x 3...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:A mutational analysis of the delta region of the Jun protein shows an inverse correlation between transforming and transactivation potential of the mutant proteins if both properties are measured in chicken embryo fibroblasts. The possibility that Jun acquires oncogenicity not by gain but by loss of function is also s...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:The p21MDA6 gene product induces cell cycle arrest in p53-null human leukemic cells exposed to differentiation stimuli. We employed an HL-60 cell line stably transfected with a p21MDA6 antisense construct to compare the effects of p21MDA6 dysregulation on the response of myeloid leukemia cells to differentiating and c...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2400625
更新日期：1997-04-01 00:00:00
abstract：:Trisomy 12 is the most common chromosomal aberration in chronic B lymphocytic leukemia (B-CLL). In this study we have investigated trisomy 12 and posed two major questions: (a) What is the origin of the third copy of chromosome 12? and (b) What is the proportion of trisomy 12 cells in malignant clones with this aberra...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:Some anaplastic large cell lymphomas (ALCLs) carry a specific chromosomal translocation, t(2;5)(p23;q35). Recently, we found a novel hyperphosphorylated 80-kDa protein tyrosine kinase, p80, in ALCLs with t(2;5). Subsequent cDNA cloning revealed p80 to be a fusion protein of two genes, the novel tyrosine kinase gene an...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Abnormalities of several cell-cycle regulatory genes including cyclin D1, p16CDKN2 and p15CDKN2B have been described in B cell non-Hodgkin's lymphoma (B-NHL). We describe a new B-NHL cell line (Granta 519), with concurrent abnormalities of the cyclin D1, pl6CDKN2 and pl5CDKN2B genes. An independent clinical case of ma...
journal_title：Leukemia
pub_type： 杂志文章
doi：10.1038/sj.leu.2400555
更新日期：1997-01-01 00:00:00
abstract：:We have investigated the involvement of tumor suppressor genes (p53 and RB1) and dominantly acting oncogenes (Ras family genes) in BCR/ABL positive and negative chronic myeloproliferative disorders (CMPD) at different stages of the disease, including 26 cases of BCR/ABL+ chronic myeloid leukemia (CML) blast crisis, 9 ...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Using the polymerase chain reaction and Southern blot analysis the expression was detected of a bcr-abl mRNA lacking abl exon a2. This was due to a corresponding unusual localization of the breakpoint in the c-abl gene and was seen in a patient with Philadelphia (Ph) chromosome positive chronic myelogeneous leukemia i...
journal_title：Leukemia
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
