Abstract:
:Connective tissue growth factor (CTGF) has been identified as a secretory protein encoded by an immediate early gene and is a member of the CCN family. In vitro CTGF directly regulates the proliferation and differentiation of chondrocytes; however, a previous study showed that it was localized only in the hypertrophic chondrocytes in the costal cartilages of E 18 mouse embryos. We described the expression of CTGF mRNA and protein in chondrocytes of different types of cartilages, including femoral growth plate cartilage, costal cartilage, femoral articular cartilage, mandibular condylar cartilage, and cartilage formed during the healing of mandibular ramus fractures revealed by in situ hybridization and immunohistochemistry. To characterize the CTGF-expressing cells, we also analyzed the distribution of the type I, type II, and type X collagen mRNA expression. Among these different types of cartilages we found distinct patterns of CTGF mRNA and protein expression. Growth plate cartilage and the costal cartilage showed localization of CTGF mRNA and protein in the hypertrophic chondrocytes that expressed type X collagen mRNA with less expression in proliferating chondrocytes that expressed type II collagen mRNA, whereas it was also expressed in the proliferating chondrocytes that expressed type I collagen mRNA in the condylar cartilage, the articular cartilage, and the cartilage appearing during fracture healing. In contrast, the growth plate cartilages or the costal cartilages were negative for type I collagen and showed sparse expression of CTGF mRNA in the proliferating chondrocytes. We found for the first time that CTGF mRNA could be differentially expressed in five different types of cartilage associated with those expressing type I collagen. Moreover, the spatial distribution of CTGF mRNA in the cartilages with type I collagen mRNA suggested its roles in the early differentiation, as well as in the proliferation and the terminal differentiation, of those cartilages.
journal_name
Bonejournal_title
Boneauthors
Fukunaga T,Yamashiro T,Oya S,Takeshita N,Takigawa M,Takano-Yamamoto Tdoi
10.1016/j.bone.2003.07.010subject
Has Abstractpub_date
2003-12-01 00:00:00pages
911-8issue
6eissn
8756-3282issn
1873-2763pii
S8756328203002758journal_volume
33pub_type
杂志文章相关文献
BONE文献大全abstract::To better understand the effects of prolonged bed-rest in women, 24 healthy women aged 25 to 40 years participated in 60-days of strict 6° head-down tilt bed-rest (WISE-2005). Subjects were assigned to either a control group (CON, n=8) which performed no countermeasure, an exercise group (EXE, n=8) undertaking a combi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.06.021
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Chronic kidney disease (CKD) is commonly associated with mineral and bone metabolism disorders, but these are less frequently studied in non-dialysis CKD patients than in dialysis patients. We examined and described international variation in mineral and bone disease (MBD) markers and their tr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115058
更新日期:2019-12-01 00:00:00
abstract::Suppression of bone remodeling by bisphosphonates leads to accumulation of microdamage in bone. If this microdamage develops due to suppressed repair of remodeling only, more potent bisphosphonates should cause more damage. In this study, we evaluated the effects of reduced bone turnover produced by a potent bisphosph...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.01.016
更新日期:2013-05-01 00:00:00
abstract::Osteogenesis imperfecta (OI) is a heritable collagen-related bone dysplasia, characterized by brittle bones with increased fracture risk that presents most severely in children. Anti-resorptive bisphosphonates are frequently used to treat pediatric OI and controlled clinical trials have shown that bisphosphonate thera...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.10.012
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:This observational safety study used national registers to compare the real world cardiovascular and skeletal safety of zoledronic acid (ZA) against oral bisphosphonates (oBP) and untreated population controls. METHODS:Propensity score matched cohort study in Sweden and Denmark. RESULTS:Matched cohort 1 in...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115296
更新日期:2020-05-01 00:00:00
abstract::High-resolution peripheral quantitative computed tomography (HR-pQCT) is clinically available today and provides a non-invasive measure of 3D bone geometry and micro-architecture with unprecedented detail. In combination with microarchitectural finite element (μFE) models it can be used to determine bone strength usin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.02.022
更新日期:2011-06-01 00:00:00
abstract::Paget's disease of bone (PDB) is a chronic bone metabolic disorder. Currently, PDB is the second most frequent bone disorder. PDB is a focal disorder affecting the skeleton segmentally but the cause of which is unknown. It has been hypothesised that somatic mutations could be responsible for the mosaicism described in...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.04.003
更新日期:2018-07-01 00:00:00
abstract::Adiponectin regulates various metabolic processes including glucose flux, lipid breakdown and insulin response. We recently reported that adiponectin receptor1 (adipoR1) activation by a small molecule reverses osteopenia in leptin receptor deficient db/db (diabetic) mice. However, the role of adiponectin in bone metab...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.08.005
更新日期:2017-12-01 00:00:00
abstract::There are conflicting reports on the influence of lean and fat mass on bone accrual during childhood. No infant's studies have been reported that describe the influence of changes in body composition with changes in bone accrual during the first year of life. The objective of this research was to test the hypothesis t...
journal_title:Bone
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.bone.2011.11.011
更新日期:2012-03-01 00:00:00
abstract::We compared the distribution of a cysteine proteinase inhibitor, cystatin C, with that of cathepsin K in osteoclasts of the mouse tibia by immunolight and immunoelectron microscopy. Light microscopically, strong immunoreactivity for cystatin C was found extracellularly along the resorption lacuna and intracellularly i...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00466-5
更新日期:2001-07-01 00:00:00
abstract::Bone morbidity associated with antineoplastic treatment is a significant health concern for postmenopausal breast cancer patients. Trials have demonstrated that adjuvant therapy with an Aromatase Inhibitor is associated with an increase in musculoskeletal disorders and bone fractures. The objective of this study was t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.09.025
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:The pathogenesis of osteonecrosis of the femoral head (ONFH) probably reflects multiple etiologies. Recent studies have explored associations between genetic mutations and/or polymorphisms and ONFH. Annexins (ANXs) have been implicated in many physiological functions, including blood coagulation, inflammation...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.03.670
更新日期:2009-07-01 00:00:00
abstract::Despite the remarkable healing potential of long bone fractures, traumatic injuries that result in critical defects require challenging reconstructive limb sparing surgery. While devitalized allografts are the gold standard for these procedures, they are prone to failure due to their limited osseointegration with the ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.10.003
更新日期:2011-03-01 00:00:00
abstract::Collagen sponge disks (6 mm diameter, 1 mm thickness) were impregnated with recombinant human bone morphogenetic protein-2 (rhBMP-2) (5 microg/disk) and implanted onto the back muscles of mice. Ten or 20 mg/kg per day of Rolipram, a selective inhibitory agent to phosphodiesterase type 4 (PDE-4), or vehicle, was inject...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00681-6
更新日期:2002-04-01 00:00:00
abstract::A predictable animal model with skeletal remodeling characteristics similar to those of humans is needed to facilitate the understanding of the mechanism of postmenopausal osteoporosis. We have utilized the ovariohysterectomized (Ovh) dog to examine cellular and biochemical responses to estrogen depletion and PTH stim...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(92)90075-8
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Patients with primary hyperparathyroidism (PHPT) generally show reduced bone mineral density (BMD) at cortical sites with relatively preserved trabecular bone. However, the increased fracture risk at all skeletal sites suggests that areal BMD probably is not effective in capturing all the determinants of bon...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.11.041
更新日期:2013-03-01 00:00:00
abstract::We have previously shown that after bone fracture, galanin (GAL) and GAL receptor expression is increased in osteoblast-like cells of callus; however, the role of elevated GAL/GAL receptors in this instance of bone injury is not known. We hypothesize that in injury, GAL may facilitate bone formation by suppressing the...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.10.018
更新日期:2007-04-01 00:00:00
abstract::The role of prostaglandins (PGs) in physiological remodeling has not yet been defined. The present study was undertaken to determine whether they intervene during the activation phase in a highly reproducible and synchronized model of bone remodeling. Indomethacin was employed to inhibit PG synthesis. This treatment t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(88)90003-8
更新日期:1988-01-01 00:00:00
abstract::In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(00)00459-2
更新日期:2001-04-01 00:00:00
abstract::Given the adaptation of bone to prevalent loading, bone loss should follow, but lag behind, the decline in physical performance during aging. Furthermore, bone responsiveness to load-induced strains is believed to decrease with aging. However, the relationship between bone and lean body ( approximately muscle) mass ap...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.07.014
更新日期:2009-11-01 00:00:00
abstract::Conventional methods used to image and quantify microdamage accumulation in bone are limited to histological sections, which are inherently invasive, destructive, two-dimensional, and tedious. These limitations inhibit investigation of microdamage accumulation with respect to volumetric spatial variation in mechanical...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.10.160
更新日期:2011-03-01 00:00:00
abstract::The role of drinking water fluoride content for prevention of osteoporosis remains controversial. Therefore, we analyzed the influence of drinking water fluoridation on the incidence of osteoporotic hip fractures and bone mineral density (BMD) in two different communities in eastern Germany: in Chemnitz, drinking wate...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00273-1
更新日期:1998-03-01 00:00:00
abstract::Identification of a reliable marker of skeletal precursor cells within calcified and soft tissues remains a major challenge for the field. To address this, we used a transgenic model in which osteoblasts can be eliminated by pharmacological treatment. Following osteoblast ablation a dramatic increase in a population o...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.04.023
更新日期:2008-09-01 00:00:00
abstract::Sclerostin monoclonal antibody (Scl-Ab) has been shown to increase bone mass and bone strength by stimulating bone formation in an ovariectomy-induced bone loss rat model. The purpose of this study was to determine the effects of Scl-Ab in a rat immobilization/disuse model in which there was both a decrease in bone fo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.09.009
更新日期:2011-02-01 00:00:00
abstract:CONTEXT:Selective serotonin reuptake inhibitors (SSRIs) are commonly prescribed medications to treat depression and anxiety. SSRIs exert their effects by inhibiting the serotonin transporter and modulating extracellular serotonin levels, a neurotransmitter that has been shown to affect bone metabolism in animals. Studi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.04.042
更新日期:2015-09-01 00:00:00
abstract::Bone structure is the key to the understanding of fracture risk. The hypothesis tested in this prospective study is that dense mandibular trabeculation predicts low fracture risk, whereas sparse trabeculation is predictive of high fracture risk. Out of 731 women from the Prospective Population Study of Women in Gothen...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.06.036
更新日期:2011-10-01 00:00:00
abstract::In elderly subjects and in particular in those with osteoporosis the evidence on age related volume changes of the hip is still very limited. Even less is known about bone changes of the femoral head. The aim of this study is to explore associations of bone size of the femoral head and neck with age in postmenopausal ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115545
更新日期:2020-10-01 00:00:00
abstract::Cathepsin K is a major osteoclastic protease. We have recently shown that overexpression of mouse cathepsin K gene in transgenic UTU17 mouse model results in high turnover osteopenia of metaphyseal trabecular bone at the age of 7 months. The present report extends these studies to a systematic analysis of cortical bon...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.02.006
更新日期:2005-05-01 00:00:00
abstract::Bone tissue undergoes permanent and lifelong remodeling with a concerted action of bone-building osteoblasts and bone-resorbing osteoclasts. A precise cooperation between those two cell types is critical in the complex process of bone renewal. Galectin-3 is a member of the β-galactoside-binding lectin family playing m...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.08.013
更新日期:2017-12-01 00:00:00
abstract::Osteoclasts are multinucleated cells of monocyte/macrophage origin that degrade bone matrix. The differentiation of osteoclasts is dependent on a tumor necrosis factor (TNF) family cytokine, receptor activator of nuclear factor (NF)-kappaB ligand (RANKL), as well as macrophage colony-stimulating factor (M-CSF). Congen...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2006.09.023
更新日期:2007-02-01 00:00:00