Quality control limits for voriconazole disk susceptibility tests on Mueller-Hinton agar with glucose and methylene blue.

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for the identification of mycobacteria. Although mycobacteria require extraction prior to MALDI-TOF MS analysis, previously published protocols have been relatively c...

journal_title：Journal of clinical microbiology

authors： Mather CA,Rivera SF,Butler-Wu SM

更新日期：2014-01-01 00:00:00

abstract：:A group of 76 consecutive human immunodeficiency virus (HIV)-positive patients with fever of unknown origin (n = 52) or fever associated with pulmonary diseases was evaluated in order to assess the usefulness of PCR with peripheral blood in the diagnosis and follow-up of visceral leishmaniasis. We identified 10 cases ...

journal_title：Journal of clinical microbiology

authors： Pizzuto M,Piazza M,Senese D,Scalamogna C,Calattini S,Corsico L,Persico T,Adriani B,Magni C,Guaraldi G,Gaiera G,Ludovisi A,Gramiccia M,Galli M,Moroni M,Corbellino M,Antinori S

更新日期：2001-01-01 00:00:00

abstract：:Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle regi...

journal_title：Journal of clinical microbiology

authors： He Y,Zhou Y,Wu H,Kou Z,Liu S,Jiang S

更新日期：2004-11-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00

abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

更新日期：1979-01-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) infection and colonization, involving MRSA strains which differ from common human health care-associated clones, have become serious emerging conditions in equine veterinary hospitals. In 2010, MRSA spa type t535 caused an outbreak involving both horses and personnel ...

journal_title：Journal of clinical microbiology

authors： Steinman A,Masarwa S,Tirosh-Levy S,Gleser D,Kelmer G,Adler A,Carmeli Y,Schwaber MJ

更新日期：2015-09-01 00:00:00

abstract：:Antibody responses of 85 patients to human immunodeficiency virus type 1 antigens were quantitated by densitometric analysis of Western blot (immunoblot) assays. All patients had been classified into the following three clinical categories: asymptomatic (ASY), acquired immunodeficiency syndrome (AIDS)-related complex ...

journal_title：Journal of clinical microbiology

authors： Schmidt G,Amiraian K,Frey H,Wethers J,Stevens RW,Berns DS

更新日期：1989-05-01 00:00:00

abstract：:Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

journal_title：Journal of clinical microbiology

authors： Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

更新日期：2010-03-01 00:00:00

abstract：:Blood isolates of Salmonella enterica serovar Typhi from two recently returned Bangladeshi patients in Kuwait were ciprofloxacin resistant, with ciprofloxacin MICs of 12 mg/liter for both isolates. Both isolates had three novel gyrA mutations (55-Leu-->Trp, 87-Asp-->Ala, and 106-Gln-->Arg) and three novel parC mutatio...

journal_title：Journal of clinical microbiology

authors： Dimitrov T,Dashti AA,Albaksami O,Udo EE,Jadaon MM,Albert MJ

更新日期：2009-01-01 00:00:00

abstract：:A new blood lysis-filtration culture technique revealed a high incidence of Staphylococcus epidermidis in the blood of patients and of healthy people. Of 2,004 blood cultures from patients, the blood lysis method grew S. epidermidis in 233 (11.6%), whereas a conventional two-bottle culture system grew this organism in...

journal_title：Journal of clinical microbiology

authors： Zierdt CH

更新日期：1983-04-01 00:00:00

abstract：:The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had...

journal_title：Journal of clinical microbiology

authors： Bolton V,Pedersen NC,Higgins J,Jennings M,Carlson J

更新日期：1987-08-01 00:00:00

abstract：:The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western co...

journal_title：Journal of clinical microbiology

authors： Saribasak H,Salih BA,Yamaoka Y,Sander E

更新日期：2004-04-01 00:00:00

abstract：:Optimal conditions for a fluorescence immunoassay of antibodies to, and surface antigens of, Actinomyces viscosus ATCC 19246 are described. In the standard fluorescence immunoassay, 10(8) colony-forming units of A. viscosus reacted with an antibody preparation, were washed, and then were treated with an excess of fluo...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-02-01 00:00:00

abstract：:This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extr...

journal_title：Journal of clinical microbiology

authors： Srilohasin P,Chaiprasert A,Tokunaga K,Nishida N,Prammananan T,Smittipat N,Mahasirimongkol S,Chaiyasirinroje B,Yanai H,Palittapongarnpim P

更新日期：2014-12-01 00:00:00

abstract：:Helicobacter pylori strains display remarkable genetic diversity, and the presence of strains bearing the toxigenic vacA s1 allele, a complete cag pathogenicity island (PAI), cagA alleles containing multiple EPIYA phosphorylation sites, and expressing the BabA adhesin correlates with development of gastroduodenal dise...

journal_title：Journal of clinical microbiology

authors： Talarico S,Gold BD,Fero J,Thompson DT,Guarner J,Czinn S,Salama NR

更新日期：2009-06-01 00:00:00

abstract：:A convenient assay combining solution hybridization and enzyme immunoassay for DNA-RNA hybrids (polymerase chain reaction-enzyme immunoassay [PCR-EIA]) was developed to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the PCR and was compared with oligomer hybridization with 32P-labeled SK19. I...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Saint-Antoine P,Olivier C,Voyer H,Kessous-Elbaz A,Berrada F,Bégin P,Giroux L,Viscidi R

更新日期：1991-11-01 00:00:00

abstract：:Strains of Candida albicans obtained from human immunodeficiency virus (HIV)-positive individuals prior to their first episode of oral thrush were already in a high-frequency mode of switching and were far more resistant to a number of antifungal drugs than commensal isolates from healthy individuals. Switching in the...

journal_title：Journal of clinical microbiology

authors： Vargas K,Messer SA,Pfaller M,Lockhart SR,Stapleton JT,Hellstein J,Soll DR

更新日期：2000-10-01 00:00:00

abstract：:Tropheryma whipplei is the causative agent of classic Whipple's disease (WD) and other clinical entities, such as localized infection. Asymptomatic carriers have also been reported, mainly based on the testing of fecal samples. Our objective was to undertake a retrospective analysis of molecular biology usage for the ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Fenollar F,Raoult D

更新日期：2012-12-01 00:00:00

abstract：:We report transmission of an azole-resistant, isogenic strain of Candida albicans in a human immunodeficiency virus (HIV)-infected family of two children with symptomatic oropharyngeal candidiasis and a mother with asymptomatic colonization over a 5-year period. These findings were confirmed by three different molecul...

journal_title：Journal of clinical microbiology

authors： Müller FM,Kasai M,Francesconi A,Brillante B,Roden M,Peter J,Chanock SJ,Walsh TJ

更新日期：1999-10-01 00:00:00

abstract：:Standard culture methods for diagnosis of Streptococcus pneumoniae pneumonia take at least 24 h. The BinaxNOW urine-based test for S. pneumoniae (BinaxNOW-SP) takes only 15 min to conduct, potentially enabling earlier diagnosis and targeted treatment. This study was conducted to assess whether the use of BinaxNOW-SP a...

journal_title：Journal of clinical microbiology

authors： Sinclair A,Xie X,Teltscher M,Dendukuri N

更新日期：2013-07-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, econom...

journal_title：Journal of clinical microbiology

authors： Piccolomini R,Di Bonaventura G,Neri M,Di Girolamo A,Catamo G,Pizzigallo E

更新日期：1999-01-01 00:00:00

abstract：:We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs wa...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-11-01 00:00:00

abstract：:In the last 2 decades, a variety of different molecular typing methods have been developed to differentiate strains of Mycobacterium avium subsp. paratuberculosis. The most successful techniques are based on insertion sequences, repetitive loci, comparative genomics, or single nucleotide polymorphisms. In the present ...

journal_title：Journal of clinical microbiology

authors： Griffiths TA,Rioux K,De Buck J

更新日期：2008-04-01 00:00:00

abstract：:The accuracy of disk susceptibility tests with ampicillin and ampicillin-sulbactam was not improved when the amount of ampicillin was increased from 10 to 20 or 30 micrograms per disk. For testing members of the family Enterobacteriaceae, ampicillin disk tests correlated better with broth microdilution tests when the ...

journal_title：Journal of clinical microbiology

authors： Barry AL,Jones RN

更新日期：1988-04-01 00:00:00

abstract：:A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

journal_title：Journal of clinical microbiology

authors： Wolfson JS,Waldron MA,Sierra LS

更新日期：1990-09-01 00:00:00

abstract：:We report disseminated adenovirus (ADV) infection in four adult cancer patients presenting with focal pulmonary consolidation. In all cases, ADV was recovered from respiratory specimens and ADV viremia (>1 × 10(5) copies/ml) was determined by a quantitative PCR assay. Despite antiviral therapy, 3 (75%) patients died. ...

journal_title：Journal of clinical microbiology

authors： Lee YJ,Palomino-Guilen P,Babady NE,Lamson DM,St George K,Tang YW,Papanicolaou GA

更新日期：2014-01-01 00:00:00

abstract：:Initially, multilocus enzyme electrophoresis was used to examine genetic relationships among 63 isolates of Mycobacterium bovis and 13 other members of the M. tuberculosis complex. The isolates were divided into five electrophoretic types, with a mean genetic diversity of 0.1. The strains were genetically homogenous, ...

journal_title：Journal of clinical microbiology

authors： Feizabadi MM,Robertson ID,Cousins DV,Hampson DJ

更新日期：1996-05-01 00:00:00

abstract：:A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

journal_title：Journal of clinical microbiology

authors： Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

更新日期：2011-10-01 00:00:00

abstract：:Microsporidia (phylum Microsproa) have recently become recognized as common opportunistic protozoans in the United States and worldwide, particularly affecting immunodeficient patients. Microsporidian organisms within the genus Encephalitozoon are the cause of nephrologic, ophthalmic, pneumologic, gastroenteric, and s...

journal_title：Journal of clinical microbiology

authors： Enriquez FJ,Ditrich O,Palting JD,Smith K

更新日期：1997-03-01 00:00:00