Relation of P-glycoprotein expression with spontaneous in vitro apoptosis in B-cell chronic lymphocytic leukemia.

Abstract:

:Prolonged lifespan of monoclonal lymphocytes in B-cell lymphocytic leukemia (B-CLL) arises from their resistance to programmed cell death. In contrast, when cultured in vitro, B-CLL tumour cells rapidly undergo apoptosis. There is mounting evidence that P-glycoprotein (P-gp), an adenosine triphosphate-binding cassette (ABC) family transporter, plays a significant role in the regulation of apoptosis induced by various stimuli. Since P-gp is commonly expressed in B-CLL cells, we aimed to establish whether its expression level influences resistance to spontaneous apoptosis in B-CLL. For that purpose, P-gp expression by UIC2 antibody staining and P-gp activity by rhodamine 123 (Rh123) efflux in presence or absence of P-gp inhibitor verapamil were studied in peripheral blood lymphocytes obtained from 43 previously untreated B-CLL patients. Simultaneously, the percentage of cells undergoing spontaneous in vitro apoptosis (apoptotic index, AI) by means of activation of caspases and annexin-V-based assays was evaluated. The AI were higher in B-CLL cells than in normal peripheral blood mononuclear cells (medians of AI 27.7% vs 3.9%, p=0.0001 and 34.7% vs 7.4%, p=0.0038, in 24 and 48-hour culture respectively). The AI were also higher among female patients as compared to male patients (medians: 29.7 vs 19.2 p=0.048). Interestingly, we found moderate inverse correlation between P-gp protein expression and AI after 24-hour culture in analysed B-CLL samples (r= -0.36, p=0.019). Moreover, P-gp positive B-CLL samples expressed significantly higher AI than P-gp negative samples with an arbitrary cut-off at Kolmogorov-Smirnov statistics D-value 0.2 (medians of AI 18.4% vs 29.7%, p=0.026). Based on these results we suggest that P-gp expression has some protective effect on B-CLL cell survival in vitro. The difference in the rates of spontaneous apoptosis among male and female patients may contribute to gender-dependent variations in clinical outcome in B-CLL.

journal_name

Neoplasma

journal_title

Neoplasma

authors

Jamroziak K,Smolewski P,Cebula B,Szmigielska-Kaplon A,Darzynkiewicz Z,Robak T

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

181-7

issue

3

eissn

0028-2685

issn

1338-4317

journal_volume

51

pub_type

杂志文章
  • Circulating immune complexes in patients with melanoma.

    abstract::Sera from 51 melanoma patients and from 55 healthy donors were examined for the presence of immune complexes (ICs) by the EA-rosette forming cell inhibition assay. Using a cut-off point of 5% rosette inhibition, ICs were found in 144 out of 213 sera from patients with melanoma (67.6%) and in 11 out of 55 control subje...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Sztaba-Kania M,Jassem J,Piskorzyńska M,Kondrat W

    更新日期:1981-01-01 00:00:00

  • Alterations in erythrocyte enzymes in cancer.

    abstract::An increase in lactate production, and in activities of phosphohexoisomerase, phosphofructokinase and pyruvate kinase was found in erythrocytes of patients with advanced cancer disease. Phosphofructokinase isolated from patients' erythrocytes showed enhanced affinity for substrate and coenzyme, diminished thermal stab...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Kwiatkowska D,Dawiskiba J,Kwiatkowska J

    更新日期:1980-01-01 00:00:00

  • Expression of cyclin A in human leukemia cell lines HL-60 and K-562 at the level of light and electron microscope by using immunocytochemical methods.

    abstract::In this study subcellular localization of cyclin A in the human promyelocytic leukemia cell line HL-60 and the human erythroleukemic K-562 cell line was examined by immunocytochemical methods. Studies were based on light and electron microscope evaluations. Cyclin A at the level of light microscope was present in 48% ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Grzanka A,Styczynski J

    更新日期:2001-01-01 00:00:00

  • Heterochromatin condensation in central and peripheral nuclear regions of maturing lymphocytes in the peripheral blood of patients suffering from B chronic lymphocytic leukemia - a cytochemical study.

    abstract::The present study was undertaken to provide complementary information on heterochromatin condensation in central and peripheral nuclear regions during maturation of human leukemic lymphocytes using simple image processing and DNA image densitometry at the single cell level. Such approach indicated that the heterochrom...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2011_06_476

    authors: Smetana K,Karhan J,Trneny M

    更新日期:2011-01-01 00:00:00

  • Co-expression of GFAP, vimentin and cytokeratins in GL-15 glioblastoma cell line.

    abstract::Glial fibrillary acidic protein (GFAP), vimentin (Vi) and cytokeratin (CK) intermediate filament (IF) proteins were studied in glioblastoma cell line GL-15. The immunofluorescence staining revealed strong positive staining for vimentin in all cultured cells. Approximately 20% of analyzed cells showed strong and 50% mo...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Perzelcová A,Máciková,Tardy M,Mráz P,Steno J,Bízik I

    更新日期:2000-01-01 00:00:00

  • Management of germ cell testicular cancer with pulmonary metastases.

    abstract::Twenty eight patients with germ cell testicular cancer pulmonary metastases received primary chemotherapy including bleomycin, etoposide, and cisplatin (BEP). Complete response was achieved in 21 (75%) patients, in 11 of them CR was achieved following chemotherapy alone. Postchemotherapy surgery of residual mass was p...

    journal_title:Neoplasma

    pub_type: 临床试验,杂志文章

    doi:

    authors: Schnorrer M,Ondrus D,Cársky S,Hornák M,Belan V,Kausitz J,Matośka J

    更新日期:1996-01-01 00:00:00

  • MicroRNA-101 regulates autophagy, proliferation and apoptosis via targeting EZH2 in laryngeal squamous cell carcinoma.

    abstract::Laryngeal carcinoma is the second commonest head and neck carcinoma globally. MicroRNA-101 (miR-101) has been reported as a tumor suppressor in multiple malignancies including laryngeal squamous cell carcinoma (LSCC). However, the roles and molecular mechanisms of miR-101 in the development of LSCC have not been fully...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2018_180811N611

    authors: Chen L,Jia J,Zang Y,Li J,Wan B

    更新日期:2019-07-23 00:00:00

  • Annexin A2 is implicated in multi-drug-resistance in gastric cancer through p38MAPK and AKT pathway.

    abstract::Studies have shown that Annexin A2 (ANXA2) is related with tumor proliferation, apoptosis, differentiation, invasion, migration, and drug resistance. The purpose of this study was to investigate the role and its mechanisms of ANXA2 in multi-drug-resistance (MDR) in gastric cancer. ANXA2 expression in both gastric canc...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2014_078

    authors: Zhang ZD,Li Y,Fan Q,Zhao B,Tan B,Zhao XF

    更新日期:2014-01-01 00:00:00

  • Expression of serine and glycine-related enzymes in phyllodes tumor.

    abstract:UNLABELLED:Expression patterns of proteins involved in serine and glycine metabolism, and correlations of these patterns with clinicopathologic factors in phyllodes tumor were investigated. Tissue microarrays were prepared from 203 phyllodes tumors (PT) and stained with antibodies specific for glycine decarboxylase (GL...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2014_069

    authors: Kwon JE,Kim DH,Jung WH,Koo JS

    更新日期:2014-01-01 00:00:00

  • Lymphocyte populations in premalignant lesions and cancer of the oral cavity.

    abstract::A comparison of the absolute numbers of leukocytes, proportions, and absolute numbers of various lymphocyte populations in patients with precancerous lesions and cancer of the oral cavity along with normal controls was carried out. Total leukocytes and lymphocytes showed reduction in their numbers in the patient group...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Pillai MR,Balaram P,Abraham T,Nair MK

    更新日期:1987-01-01 00:00:00

  • Endocrine factors in the etiology of endometrial carcinoma.

    abstract::In the blood of 39 women with endometrial carcinoma the FSH, LH, estrone, androstendion, estradiol and dehydroepiandrosterone were determined by the RIA method. Compared to the control group the estrone (303.3 +/- 44.3 pmol/l), estradiol (165.2 +/- 29.4 pmol/l) and the androstendion (5.24 +/- 0.44 nmol/l) were signifi...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Gimes G,Szarvas Z,Siklósi G

    更新日期:1986-01-01 00:00:00

  • Combined therapy of B16(F10) murine melanoma using E. coli cytosine deaminase gene and murine interleukin-4 gene.

    abstract::This paper summarizes preliminary results of combining suicide gene strategy (E. coli cytosine deaminase gene--CD) with immunotherapy (murine interleukin-4 gene) for treatment of experimental B16(F10) melanomas implanted into C57Bl/6 mice. The best therapeutic results, inhibition of tumor growth and prolonged survival...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Missol-Kolka E,Sochanik A,Szala S

    更新日期:1998-01-01 00:00:00

  • Sensitivity of TSTA and species-specific cell membrane antigens of tumor cells to glutaraldehyde treatment.

    abstract::In vivo immunogenicity and in vitro species-specific membrane antigens in tumor cells treated or untreated with glutaraldehyde (GA) were studied. Two different syngeneic Syrian hamster transplantable tumor cell lines (spontaneous liver cancer and SV40-induced sarcoma) not only lost immunogenicity after GA treatment bu...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Kluchareva TE,Matveeva VA,Deichman GI

    更新日期:1978-01-01 00:00:00

  • Comparison of two non-anthracycline-containing regimens for elderly patients with diffuse large-cell non Hodgkin's lymphoma--possible pitfalls in results reporting and interpretation.

    abstract::Age over 65 years is a risk factor per se for doxorubicin administration, and coexisting diseases pose additional problems. There is still controversy whether chemotherapy regimens for elderly patients with aggressive NHL should be full-dose doxorubicin containing or whether development of non-anthracycline containing...

    journal_title:Neoplasma

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Jelić S,Milanović N,Tomasević Z,Matković S,Gavrilović D

    更新日期:1999-01-01 00:00:00

  • Tumor microenvironment and the role of mesenchymal stromal cells.

    abstract:UNLABELLED:Solid tumors are generally composed of two major components: heterogeneous malignant cells and non-malignant stromal part. The latter comprises several types of non-malignant cells of mesenchymal, endothelial and immune origin and together with the extracellular matrix significantly affects the biological pr...

    journal_title:Neoplasma

    pub_type: 杂志文章,评审

    doi:10.4149/neo_2013_001

    authors: Kucerova L,Skolekova S

    更新日期:2013-01-01 00:00:00

  • Distribution of 64copper in the blood and different tissues of mice bearing induced fibrosarcoma.

    abstract::To understand the nature of copper distribution during malignancy, 27 strains A/RB mice with fibrosarcoma were used. Tracer methodology with radioactive isotope of copper (64Cu) was applied. In the study made at different time intervals after isotope administration whole blood plasma and different tissues like liver, ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Chakravarty PK,Ghosh A,Chowdhury JR

    更新日期:1985-01-01 00:00:00

  • Effect of chemotherapy on the lymph node and bone marrow cell chromosomes in patients with Hodgkin's disease.

    abstract::Chromosomal aberrations in bone marrow and lymph node cells have been investigated in 47 patients with lymphogranulomatosis 24--72 hr after administration of a single therapeutic dose of cyclophosphan and natulan. It is shown that the injurious action of cyclophosphan and natulan lasts more than 72 hr. An analysis of ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Neistadt EL,Gershanovich ML,Kolygin BA,Ogorodnikova BN,Fedoreev GA,Chekharina EA,Filov VA

    更新日期:1978-01-01 00:00:00

  • Flotillin 1 is differentially expressed in human epithelial ovarian tumors.

    abstract::Although Flotillin 1 (FLOT1) is highly expressed in various human cancers, its relationship with ovarian cancer (OC) remains unknown. This study determines FLOT1 expression in human ovarian tumors and examines its effect on OC   cell proliferation. FLOT1 protein expression was assessed in a tissue microarray by immuno...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2018_170714N483

    authors: Li J,Zuo X,Shi J,Zhang J,Duan X,Xu G

    更新日期:2018-01-01 00:00:00

  • Mitochondrial transmembrane potential in spontaneous and camptothecin-induced apoptosis of melanotic and amelanotic melanoma cells.

    abstract::In this work we tried to estimate the role of mitochondria in the ability of cells of two: melanotic and amelanotic transplantable melanoma lines to undergo spontaneous and camptothecin-induced apoptosis. We measured mitochondrial transmembrane potential (DeltaPsi) changes during culture without (spontaneous apoptosis...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Cichorek M,Kozłowska K,Bryl E

    更新日期:2007-01-01 00:00:00

  • Sacharose density gradient separation of Ehrlich ascites carcinoma and L-5178Y tumor cells in different cell cycle phases.

    abstract::Optimal conditions were determined for the distribution of Ehrlich ascites carcinoma (EAC) and L-5178Y mouse tumor cells, proliferating in vivo, by their age within the cell cycle by sedimentation in a buffered linear sacharose density gradient. Measurements of cell size, DNA content and incorporation of tritiated thy...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Horáková K,Fusková A,Ceglédyová N

    更新日期:1976-01-01 00:00:00

  • Identification of differential proteins in colon cancer SW480 cells with HIF1-alpha silence by proteome analysis.

    abstract::Hypoxia is one of the basic characteristics of the colorectal cancer and HIF1 plays a central role in tumor hypoxia adaptation and controls the expression of a variety of genes. But it had not reached a comprehensive understanding of them in colorectal cancer in hypoxia. This study silenced HIF1-alpha, which is the re...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2010_04_299

    authors: Wu XY,Fu ZX,Wang XH,Shen W

    更新日期:2010-01-01 00:00:00

  • DNA methylation patterns of SOCS1 gene in peripheral blood identifies risk loci associated with Bladder cancer based on principal component analysis.

    abstract::Bladder cancer (BCa) is a common carcinoma of the urinary tract which occurs in the bladder mucosa. In recent years, people have recognized that epigenetic changes such as DNA methylation play important roles in the development of BCa, but the specific mechanism is unclear. In this study we detected the methylation ra...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2020_200928N1035

    authors: Guan H,Lu J,Qu M,Meng L,Guo Y,Li X,Shen H

    更新日期:2021-01-14 00:00:00

  • Anti-tumor immunity elicited by cross-linking vaccine heat shock protein 72 and alpha-fetoprotein epitope peptide.

    abstract::Hepatocellular carcinoma (HCC) is one of the most common malignancies over the world. Alpha-fetoprotein (AFP) is an oncofetal protein during HCC development, which could generate weaker and less reproducible antitumor protection, and may serve as a target for immunotherapy. Therefore, it is imperative to enhance its i...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2015_085

    authors: Li Z,Wang XP,Lin HP,Xu B,Zhao Q,Qi BN,Yang YX,Wang ZR

    更新日期:2015-01-01 00:00:00

  • Prognostic significance of mitotic and apoptotic index and the DNA cytometry in head and neck cancer.

    abstract::The lack of suitable criteria to predict the response to chemo- and or radiotherapy for individual patients with squamous cell carcinoma of the head and neck (HNSCC) remains still a major problem. This study was conducted to analyze prognostic significance of mitotic and apoptotic index and the DNA flow cytometric ana...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Smilek P,Dusek L,Veselý K,Rottenberg J,Kostrica R

    更新日期:2005-01-01 00:00:00

  • Administration of liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE) and diclofenac in the combination attenuates their anti-tumor activities.

    abstract::The anti-tumor effects of i.p. administered cyclooxygenase inhibitor - diclofenac and i.v. administered liposomal muramyl tripeptide phosphatidylethanolamine (MTP-PE) were investigated using a s.c. growing murine fibrosarcoma tumor. Tumor growth was assessed by measuring tumor volumes and survival of the mice. Both of...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Fedorocko P,Hoferová Z,Hofer M,Brezáni P

    更新日期:2003-01-01 00:00:00

  • HELQ in cancer and reproduction.

    abstract::Helicase POLQ-like (HELQ), an ATP-dependent 3'-5' DNA helicase, plays pivotal roles in DNA processing, such as DNA replication, recombination and repair. The recent discovery of HELQ in mouse model and human cancer cell reveal that genetic alteration of HELQ is involved in cancer susceptibility and subfertility. Here ...

    journal_title:Neoplasma

    pub_type: 杂志文章,评审

    doi:10.4149/neo_2016_601

    authors: Han X,Zhao L,Li X

    更新日期:2016-01-01 00:00:00

  • The potential of ciRS-7 for predicting onset and prognosis of cervical cancer.

    abstract::Considering the potency of circRNAs in manifesting neoplastic progression, we attempted to explore the feasibility of applying ciRS-7 for diagnosis and prognosis estimation of cervical cancer (CC). Here 352 CC patients, 204 cervical intraepithelial neoplasia (CIN) patients and 227 healthy controls were recruited. The ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:10.4149/neo_2019_190415N334

    authors: Zhou Y,Shen L,Wang YZ,Zhou CC

    更新日期:2020-03-01 00:00:00

  • Analysis of surface and cytoplasmic immunoglobulin light/heavy chains by flow cytometry using a lysed-whole-blood technique: Implications for the differential diagnosis of B-cell malignancies.

    abstract::The study was aimed at the proper detection of surface and cytoplasmic clonal Ig light/heavy chains in the frame of multiparameter flow cytometry analysis of some B-cell malignancies. An exact direct evidence has been obtained that the leukemia cells following staining by antibodies to immunoglobulins will need to be ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Babusíková O,Stevulová L

    更新日期:2004-01-01 00:00:00

  • Stepwise transition of aggregate structure of high-molecular-weight avian myeloblastosis virus RNA. Mode of releasing of associated 4S RNA.

    abstract::Mode of releasing of associated 4S RNA species was studied during a controlled transition of aggregate structure of high-molecular-weight AMV-RNA. It has been found that associated 4S RNA constitutes 2.5% of 60S AMV-RNA complex. Approximately 60% of associated 4S RNA is successively released during treatment of viral ...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Trávnícek M,Ríman J

    更新日期:1976-01-01 00:00:00

  • Combined radioprotective effect of Broncho-Vaxom and WR-2721 on hemopoiesis and circulating blood cells.

    abstract::Possibilities of combined radioprotection, using preirradiation WR-2721 administration and post- or preirradiation Broncho-Vaxom administration in lethally whole-body gamma-irradiated mice were investigated. Combined modality treatments were more effective than individual treatments alone. The ameliorative influence o...

    journal_title:Neoplasma

    pub_type: 杂志文章

    doi:

    authors: Macková NO,Fedorocko P

    更新日期:1995-01-01 00:00:00