Cardioprotective effects of platelets against ischaemia-reperfusion injury are related in part to platelet glutathione redox cycle.


OBJECTIVE:Washed platelets have been shown to protect isolated buffer perfused rat hearts from injury and dysfunction following ischaemia-reperfusion. This study was designed to investigate whether platelet glutathione redox cycle contributes to the protective effect of platelets. METHODS:Isolated rat hearts were perfused with Krebs-Henseleit buffer alone, buffer containing platelets, or buffer containing platelets pretreated with inhibitors of glutathione redox cycle. All hearts were subjected to 40 min of global ischaemia followed by 30 min of reperfusion. RESULTS:Following ischaemia and reperfusion, hearts perfused with buffer alone showed a marked reduction in force of cardiac contraction and heart rate, an increase in coronary perfusion pressure and diastolic tone, and a release of creatine kinase in the coronary effluent. Myocardial creatine kinase, superoxide dismutase activity, and glutathione content all fell. Perfusion of hearts with washed platelets provided significant protection from ischaemia-reperfusion related myocardial injury and dysfunction. The cardioprotective effects of platelets were attenuated when they were preincubated with the glutathione redox cycle inhibitors dehydroepiandrosterone, 1,3-bis (2-chloroethyl)-1-nitrosurea, or 1-chloro-2,4-dinitrobenzene. Pretreatment of platelets with glutathione redox cycle inhibitors did not affect the platelet mediated preservation of myocardial superoxide dismutase activity. CONCLUSIONS:Platelet glutathione redox cycle contributes in part to the cardioprotective effects of platelets against ischaemia-reperfusion injury and dysfunction in the isolated rat heart.


Cardiovasc Res


Cardiovascular research


Yang BC,Mehta A,Mehta JL




Has Abstract


1994-10-01 00:00:00














  • Activation of vascular BK channels by docosahexaenoic acid is dependent on cytochrome P450 epoxygenase activity.

    abstract:AIMS:n-3 Polyunsaturated fatty acids (PUFAs) are known to protect the cardiovascular system and improve blood pressure control. These important dietary constituents are converted into bioactive metabolites, but their role in regulation of the cardiovascular system is unclear. In particular, the functions of the cytochr...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Wang RX,Chai Q,Lu T,Lee HC

    更新日期:2011-05-01 00:00:00

  • Pretreatment with the adenosine A1 selective agonist, 2-chloro-N6-cyclopentyladenosine (CCPA), causes a sustained limitation of infarct size in rabbits.

    abstract:OBJECTIVE:The highly selective adenosine A1 receptor agonist, 2-chloro-N6-cyclopentyl-adenosine (CCPA), has been shown to be as cardioprotective as ischaemic preconditioning when evaluated with an early staining method using tetrazolium. However, tetrazolium-positive tissue measured 3 h after reperfusion may still over...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Tsuchida A,Liu GS,Wilborn WH,Downey JM

    更新日期:1993-04-01 00:00:00

  • Disrupted Slit-Robo signalling results in membranous ventricular septum defects and bicuspid aortic valves.

    abstract:AIMS:The mesenchymal cushions lining the early embryonic heart undergo complex remodelling to form the membranous ventricular septum as well as the atrioventricular and semilunar valves in later life. Disruption of this process underlies the most common congenital heart defects. Here, we identified a novel role for Sli...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Mommersteeg MT,Yeh ML,Parnavelas JG,Andrews WD

    更新日期:2015-04-01 00:00:00

  • Strategies to achieve coronary arterial plaque stabilization.

    abstract::Acute coronary syndromes result from fissure, erosion or rupture of a vulnerable atherosclerotic plaque. The characteristics of a vulnerable plaque include a large lipid pool, an abundance of inflammatory cells and mediators, a reduced smooth muscle cell and collagen content and a thin overlying fibrous cap. Potential...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Rabbani R,Topol EJ

    更新日期:1999-02-01 00:00:00

  • The effects of sepsis on endothelium and clinical implications.

    abstract:ABSTRACT:Sepsis accounts for nearly 700 000 deaths in Europe annually and is caused by an overwhelming host response to infection resulting in organ failure. The endothelium is an active contributor to sepsis and as such represents a major target for therapy. During sepsis, endothelial cells amplify the immune response...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Dolmatova EV,Wang K,Mandavilli R,Griendling KK

    更新日期:2021-01-01 00:00:00

  • Deletion of neuronal NOS prevents impaired vasodilation in septic mouse skeletal muscle.

    abstract:OBJECTIVE:Sepsis-stimulated nitric oxide (NO) production impairs arteriolar responsiveness in skeletal muscle. Using wild type (WT), eNOS(-/-), iNOS(-/-) and nNOS(-/-) mice, we aimed to determine the key nitric oxide synthase (NOS) isoenzyme(s) responsible for the arteriolar hyporesponsiveness to acetylcholine (ACh) in...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Lidington D,Li F,Tyml K

    更新日期:2007-04-01 00:00:00

  • Rho-kinase-mediated pathway induces enhanced myosin light chain phosphorylations in a swine model of coronary artery spasm.

    abstract:OBJECTIVE:We recently demonstrated in our swine model of coronary artery spasm that enhanced myosin light chain (MLC) phosphorylations (both MLC mono- and diphosphorylations) play a central role in the pathogenesis of the spasm. However, the molecular mechanism for and the phosphorylation sites for the enhanced MLC pho...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Shimokawa H,Seto M,Katsumata N,Amano M,Kozai T,Yamawaki T,Kuwata K,Kandabashi T,Egashira K,Ikegaki I,Asano T,Kaibuchi K,Takeshita A

    更新日期:1999-09-01 00:00:00

  • Effect of sustained stretch on dispersion of ventricular fibrillation intervals in normal rabbit hearts.

    abstract:OBJECTIVE:To determine the effect of acute left ventricular dilatation on refractoriness in normal hearts. METHODS:During sustained ventricular fibrillation (VF) in isolated perfused hearts, recording of local activation time yields VF intervals which provide an index of local refractoriness. Simultaneous measurement ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Burton FL,Cobbe SM

    更新日期:1998-08-01 00:00:00

  • Global variations in the prevalence, treatment, and impact of atrial fibrillation in a multi-national cohort of 153,152 middle-aged individuals.

    abstract:AIMS:To compare the prevalence of electrocardiogram (ECG)-documented atrial fibrillation (or flutter) (AF) across eight regions of the world, and to examine anti-thrombotic use and clinical outcomes. METHODS AND RESULTS:Baseline ECGs were collected in 153,152 middle-aged participants (ages 35 to 70 years) to document ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Joseph PG,Healey JS,Raina P,Connolly SJ,Ibrahim Q,Gupta R,Avezum A,Dans AL,Lopez-Jaramillo P,Yeates K,Teo K,Douma R,Bohonar A,Chifamba J,Lanas F,Dagenais GR,Lear SA,Kumar R,Kengne AP,Keskinler M,Mohan V,Mony P,

    更新日期:2020-08-10 00:00:00

  • The atherogen 3-methylcholanthrene induces multiple DNA adducts in mouse aortic smooth muscle cells: role of cytochrome P4501B1.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Moorthy B,Miller KP,Jiang W,Ramos KS

    更新日期:2002-03-01 00:00:00

  • Hypercapnic acidosis and dimethyl amiloride reduce reperfusion induced cell death in ischaemic ventricular myocardium.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Kaplan SH,Yang H,Gilliam DE,Shen J,Lemasters JJ,Cascio WE

    更新日期:1995-02-01 00:00:00

  • Amplification by phenylephrine and serotonin of coronary vasoconstriction induced by a high arterial blood oxygen tension.

    abstract:OBJECTIVE:The aim was to investigate the effect on the coronary network of the interaction between high arterial blood oxygen tension (PaO2) and stimulation by the alpha adrenergic agonist phenylephrine or by serotonin in an isolated, blood perfused rabbit heart preparation. METHODS:Fresh pig erythrocytes in Krebs-Hen...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Mouren S,Vicaut E,Charansonney O,Duvelleroy M

    更新日期:1994-09-01 00:00:00

  • Silencing TRPM7 promotes growth/proliferation and nitric oxide production of vascular endothelial cells via the ERK pathway.

    abstract:AIMS:The presence and potential function of transient receptor potential melastatin 7 (TRPM7), a Ca2+-permeable non-selective cation channel of the TRP channel superfamily in human vascular endothelial cells, were examined. METHODS AND RESULTS:Whole-cell patch-clamp recordings showed outward-rectifying currents in hum...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Inoue K,Xiong ZG

    更新日期:2009-08-01 00:00:00

  • The angiotensin II type 2 receptor activates flow-mediated outward remodelling through T cells-dependent interleukin-17 production.

    abstract:AIMS:The angiotensin II type 1 receptor (AT1R) through the activation of immune cells plays a key role in arterial inward remodelling and reduced blood flow in cardiovascular disorders. On the other side, flow (shear stress)-mediated outward remodelling (FMR), involved in collateral arteries growth in ischaemic disease...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Caillon A,Grenier C,Grimaud L,Vessieres E,Guihot AL,Blanchard S,Lelievre E,Chabbert M,Foucher ED,Jeannin P,Beauvillain C,Abraham P,Loufrani L,Delneste Y,Henrion D

    更新日期:2016-10-01 00:00:00

  • Left atrial pacing induces memory and is associated with atrial tachyarrhythmias.

    abstract:OBJECTIVE:Transiently altering the atrial activation sequence induces atrial memory, manifested as an altered atrial gradient as measured in electrocardiographic XYZ leads. We hypothesized that protracted periods of left atrial impulse initiation alter the atrial gradient in a manner predictive of arrhythmias. METHODS...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Chandra P,Rosen TS,Herweg B,Danilo P Jr,Rosen MR

    更新日期:2003-11-01 00:00:00

  • Aging, telomeres, and atherosclerosis.

    abstract::Although the level and pace of population aging display high geographical variability, virtually all countries have been experiencing growth in their elderly population, particularly in developed nations. Because aging is a major risk factor for atherosclerosis and associated disease, it is of up most importance to un...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Edo MD,Andrés V

    更新日期:2005-05-01 00:00:00

  • The aorta can act as a site of naïve CD4+ T-cell priming.

    abstract:AIMS:Aortic adaptive immunity plays a role in atherosclerosis; however, the precise mechanisms leading to T-cell activation in the arterial wall remain poorly understood. METHODS AND RESULTS:Here, we have identified naïve T cells in the aorta of wild-type and T-cell receptor transgenic mice and we demonstrate that naï...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: MacRitchie N,Grassia G,Noonan J,Cole JE,Hughes CE,Schroeder J,Benson RA,Cochain C,Zernecke A,Guzik TJ,Garside P,Monaco C,Maffia P

    更新日期:2020-02-01 00:00:00

  • Vasopressin-induced coronary constriction at low perfusion pressures.

    abstract::We studied the effects of intracoronary vasopressin on the relationship between pressure and flow in the coronary circulation of anaesthetised swine. In addition to measurements at control levels, diastolic pressure-flow relationships were constructed from steady-state points below a coronary pressure of 50 mmHg, wher...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Pantely GA,Ladley HD,Anselone CG,Bristow JD

    更新日期:1985-07-01 00:00:00

  • Cardiac angiotensin converting enzyme overproduction indicates interstitial activation in renovascular hypertension.

    abstract:OBJECTIVES:Angiotensin converting enzyme (ACE) activity in the plasma does not change significantly with hypertension in two-kidney, one-clip hypertensive (2K-1C) rats. However, heart ACE activity and mRNA increase with hypertension. We measured the ACE activity and mRNA in hypertrophied hearts at different times after...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Challah M,Nicoletti A,Arnal JF,Philippe M,Laboulandine I,Allegrini J,Alhenc-Gelas F,Danilov S,Michel JB

    更新日期:1995-08-01 00:00:00

  • Tubulin polymerization modifies cardiac sodium channel expression and gating.

    abstract:AIMS:Treatment with the anticancer drug taxol (TXL), which polymerizes the cytoskeleton protein tubulin, may evoke cardiac arrhythmias based on reduced human cardiac sodium channel (Na(v)1.5) function. Therefore, we investigated whether enhanced tubulin polymerization by TXL affects Na(v)1.5 function and expression and...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Casini S,Tan HL,Demirayak I,Remme CA,Amin AS,Scicluna BP,Chatyan H,Ruijter JM,Bezzina CR,van Ginneken AC,Veldkamp MW

    更新日期:2010-03-01 00:00:00

  • Post implantation viability in canine allograft heart valves.

    abstract::Known viable allograft canine pulmonary valves were inserted in an isotopic position ensuring normal physiological function and simulating the clinical situation. Valves removed at periods up to eight weeks were assessed for viability using previously established criteria of tissue culture and protein and collagen syn...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Wheatley DJ,McGregor CG

    更新日期:1977-01-01 00:00:00

  • Physiological And Pathological Roles Of Protein Kinase A In The Heart.

    abstract::Protein kinase A (PKA) is a central regulator of cardiac performance and morphology. Myocardial PKA activation is induced by a variety of hormones, neurotransmitters and stress signals, most notably catecholamines secreted by the sympathetic nervous system. Catecholamines bind β-adrenergic receptors to stimulate cAMP-...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Liu Y,Chen J,Fontes SK,Bautista EN,Cheng Z

    更新日期:2021-01-23 00:00:00

  • Basilar arterial construction caused by intracisternal NG-nitro-L-arginine in anesthetized monkeys.

    abstract:OBJECTIVES:The present study was designed to determine whether tonic nitric oxide (NO)-mediated vasodilator innervation participates in basilar arterial dilatation in the anesthetized Japanese monkey. METHODS:The basilar arterial diameter was angiographically measured, and NG-nitro-L-arginine (L-NNA), a nitric oxide s...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Okamura T,Ayajiki K,Toda N

    更新日期:1995-11-01 00:00:00

  • Sarcoplasmic reticulum Ca2+-ATPase modulates cardiac contraction and relaxation.

    abstract::The cardiac SR Ca(2+)-ATPase (SERCA2a) regulates intracellular Ca(2+)-handling and thus, plays a crucial role in initiating cardiac contraction and relaxation. SERCA2a may be modulated through its accessory phosphoprotein phospholamban or by direct phosphorylation through Ca(2+)/calmodulin dependent protein kinase II ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Frank KF,Bölck B,Erdmann E,Schwinger RH

    更新日期:2003-01-01 00:00:00

  • Effects of oxidised low density lipoprotein on dendritic cells: a possible immunoregulatory component of the atherogenic micro-environment?

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Alderman CJ,Bunyard PR,Chain BM,Foreman JC,Leake DS,Katz DR

    更新日期:2002-09-01 00:00:00

  • Rate of reflow and reperfusion induced arrhythmias: studies with dual coronary perfusion.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Ibuki C,Hearse DJ,Avkiran M

    更新日期:1992-04-01 00:00:00

  • Contraction in cardiac endothelial cells contributes to changes in capillary dimensions following ischaemia and reperfusion.

    abstract:OBJECTIVE:Ischaemia followed by reperfusion brings about a reduction in cardiac capillary cross-sectional dimensions which is consistent with constriction. The aim of this study was to test the hypothesis that the reduction in cardiac capillary dimensions that occurs in ischaemia and reperfusion is caused by endothelia...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Glyn MC,Ward BJ

    更新日期:2000-11-01 00:00:00

  • Effect of acute anaesthesia on synthesis of contractile and non-contractile proteins of heart muscle and mixed proteins of types I and II fibre rich skeletal muscles of rat.

    abstract:STUDY OBJECTIVE:The aim of the study was to determine whether the deleterious effects of anaesthesia on cardiac muscle function were due to disturbance of protein synthesis. Comparative investigations were made on anaerobic and aerobic skeletal muscles, and plasma insulin and growth hormone levels were also measured to...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Siddiq T,Richardson PJ,Hashim IA,Preedy VR

    更新日期:1991-04-01 00:00:00

  • Soluble E-selectin, ICAM-1 and VCAM-1 levels in systemic and coronary circulation in patients with variant angina.

    abstract:OBJECTIVE:The purpose of this study was to assess whether the plasma levels of soluble adhesion molecules including E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are elevated in patients with variant angina and whether they are released in the coronary circulation...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Miwa K,Igawa A,Inoue H

    更新日期:1997-10-01 00:00:00


    abstract:AIM:Diabetes is a conventional risk factor for atherosclerotic cardiovascular disease and myocardial infarction (MI) is the most common cause of death among these patients. Mesenchymal stromal cells (MSCs) in patients with type 2 diabetes (T2DM) and atherosclerosis have impaired ability to suppress activated T-cells (i...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Mancini OK,Huynh DN,Menard L,Shum-Tim D,Ong H,Marleau S,Colmegna I,Servant MJ

    更新日期:2020-04-27 00:00:00