Abstract:
OBJECTIVES:Cardiovascular disease is rare in premenopausal women, but increases after the menopause when hormone replacement therapy reduces coronary events. Vascular smooth muscle cell (SMC) proliferation and migration occur in atherosclerosis, restenosis and venous graft disease. We studied the effects of 17 beta-estradiol on SMC proliferation and migration. METHODS:SMC were cultured from saphenous veins of postmenopausal women and age-matched men. Cell growth was determined by 3H-thymidine incorporation and cell counting. Migration of SMC was assessed in 4-well chambers. SMC were seeded in one corner and PDGF-BB in filter paper glued onto the opposite wall. RESULTS:PDGF-BB (5 ng/ml for 24 h) similarly stimulated 3H-thymidine incorporation in female (511 +/- 57%; n = 8) and male (528 +/- 62%; n = 12) SMC. This was reduced by 17 beta-estradiol (10(-8)-10(-6) M; female 313 +/- 52%; male 337 +/- 54%; P < 0.05). PDGF-BB increased the number of SMC (P < 0.0001 at 10 days) obtained from females (153 +/- 3%; n = 5) and males (150 +/- 4%; n = 5), which was inhibited by 17 beta-estradiol (10(-6) M; female 134 +/- 7%; male 128 +/- 5%; P < 0.05). Similar results were obtained with basic fibroblast growth factor. In contrast to 17 beta-estradiol, another steroid (dexamethasone) had no effects on 3H-thymidine incorporation in these cells stimulated with PDGF-BB, PDGF-BB (0.01-1 ng) stimulated SMC migration (P < 0.05) which was inhibited by 17 beta-estradiol (10(-10)-10(-6) M; n = 5; P < 0.005). CONCLUSION:17 beta-Estradiol inhibits growth-factor-induced SMC proliferation and migration regardless of gender. These effects of 17 beta-estradiol may contribute to its cardiovascular protective properties in postmenopausal women during replacement therapy.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Dai-Do D,Espinosa E,Liu G,Rabelink TJ,Julmy F,Yang Z,Mahler F,Lüscher TFsubject
Has Abstractpub_date
1996-11-01 00:00:00pages
980-5issue
5eissn
0008-6363issn
1755-3245pii
0008636396001496journal_volume
32pub_type
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journal_title:Cardiovascular research
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journal_title:Cardiovascular research
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journal_title:Cardiovascular research
pub_type: 杂志文章
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/cvp252
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/s0008-6363(98)00119-9
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doi:10.1016/s0008-6363(01)00372-8
更新日期:2001-11-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
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更新日期:2017-11-01 00:00:00
abstract::Vascular smooth muscle cells (vSMCs) play a crucial role in both the pathogenesis of Aneurysms and Dissections of the ascending thoracic aorta (TAAD) in humans and in the associated adaptive compensatory responses, since thrombosis and inflammatory processes are absent in the majority of cases. Aneurysms and dissectio...
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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更新日期:2014-04-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/11.4.277
更新日期:1977-07-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/28.9.1326
更新日期:1994-09-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:2007-01-15 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/18.4.220
更新日期:1984-04-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章,评审
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更新日期:2009-09-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/27.5.801
更新日期:1993-05-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:2002-12-01 00:00:00
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doi:10.1016/j.cardiores.2005.11.020
更新日期:2006-03-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/s0008-6363(97)00189-2
更新日期:1997-11-01 00:00:00
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journal_title:Cardiovascular research
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doi:10.1093/cvr/25.5.353
更新日期:1991-05-01 00:00:00