Abstract:
:The effects of recombinant human insulin-like growth factor I (IGF-I) on both vasoactive intestinal peptide (VIP) and PRL production and gene expression were studied using rat anterior pituitary cell cultures grown in serum-free defined medium. We also examined whether pituitary VIP could be involved in the PRL response to IGF-I and hence in a paracrine regulatory system. Exposure of cultured anterior pituitary cells to IGF-I (2.6 nM) for 3 h caused a significant decrease in both VIP content and media PRL. Treatment with IGF-I (from 0.65-5.2 nM) for 48 h increased VIP production and VIP messenger RNA (mRNA) accumulation, whereas only an increase in media and intracellular PRL content without changes in mRNA was observed. In all these experiments, IGF-I led to a decrease in both GH secretion and expression. Immunoglobulins G purified from VIP antiserum inhibited IGF-I-induced PRL release without affecting intracellular and mRNA levels. The inhibition of both GH secretion and gene expression induced by IGF-I was not blocked by VIP antiserum. In conclusion, these results indicate that IGF-I induces VIP gene expression, and its secretion and also increases PRL secretion. The effect of IGF-I on PRL release is specifically mediated by VIP through a paracrine or autocrine mechanism.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lara JI,Lorenzo MJ,Cacicedo L,Tolón RM,Balsa JA,López-Fernández J,Sánchez-Franco Fdoi
10.1210/endo.135.6.7988440subject
Has Abstractpub_date
1994-12-01 00:00:00pages
2526-32issue
6eissn
0013-7227issn
1945-7170journal_volume
135pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Ovarian hormones, including 17β-estradiol, are implicated in numerous physiological processes, including sleep. Beginning at puberty, girls report more sleep complaints than boys, which is maintained throughout the reproductive life stage. Sleep problems are exacerbated during the menopausal transition, evidenced by g...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqaa128
更新日期:2020-09-01 00:00:00
abstract::GH and IGF-I are potent regulators of muscle growth and function. Although IGF-I is known to mediate many of the effects of GH, it is not yet clear whether all effects of GH are completely dependent on the IGF-I system. To evaluate the biological effects of the GH/IGF-I axis on muscle growth, we administrated recombin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0906
更新日期:2005-04-01 00:00:00
abstract::The disappearance of 125-I-ACTH from the circulation of 4 pregnant and fetal sheep has been followed after a single injection to the ewe or fetus. The mean metabolic clearance rate for the fetus and the ewe was 55 and 34 ml/min/kg respectively, giving a half-life in each case of about 1 min. The higher fetal than mate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-1-231
更新日期:1975-01-01 00:00:00
abstract::Hypophysectomized rats bearing three transplanted pituitaries under the kidney capsule responded to synthetic lysine vasopressin or pitressin with a significant elevation of plasma corticosterone, whereas hypophysectomized rats with no grafts did not. This response was completely abolished by pretreatment of animals w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-3-906
更新日期:1978-09-01 00:00:00
abstract::We studied the effects of vitamin D deficiency and its correction by vitamin D or calcium-lactose supplementation on vitamin D receptor (VDR) expression in skin keratinocytes, kidney, and duodenum of adult rats. VDR messenger RNA (mRNA) was assayed by Northern blot, and VDR protein was determined immunocytochemically....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5903
更新日期:1998-04-01 00:00:00
abstract::Arginine-vasopressin (AVP) acts on vasoconstriction and diuresis through two different types of receptors (V1 and V2, respectively). Since AVP also modifies ACTH release, we have attempted to determine which class of receptors mediates the capacity of AVP to increase ACTH secretion and to potentiate the effect of cort...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-882
更新日期:1984-09-01 00:00:00
abstract::To identify genes involved in regulated insulin secretion, we have established and characterized two sublines derived from the mouse pancreatic beta-cell line MIN6, designated B1 and C3. They have a similar insulin content, but differ in their secretory properties. B1 responded to glucose in a concentration- and cell ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220916
更新日期:2003-04-01 00:00:00
abstract::To examine the mechanism of estrogen's direct stimulation of steroidogenesis in the rabbit corpus luteum, we tested the hypothesis that the effect of estrogen on progestin production occurs at the site of processing of the precursor for pregnenolone (i.e. cholesterol) in the mitochondrion. For this purpose, we manipul...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1948
更新日期:1988-05-01 00:00:00
abstract::As a metabolic sensor, the serine/threonine protein kinase AMP-activated protein kinase (AMPK) promotes the adaptation of cells to signals arising from nutrients, hormones, and growth factors. The ability of IGF-I to stimulate protein synthesis is suppressed by AMPK, therefore, these studies were undertaken to determi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-0155
更新日期:2011-08-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) is a multifunctional cytokine that is thought to play a major role in the regulation of growth and differentiation of thyroid cells. However, little is known of its detailed mechanisms of action in thyrocytes. We have therefore studied the molecular mechanisms of TGF-beta1 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7918
更新日期:2001-01-01 00:00:00
abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1336
更新日期:2011-12-01 00:00:00
abstract::GnRH release in the median eminence (ME) is the central output for control of reproduction. GnRH processes in the preoptic area (POA) also release GnRH. We examined region-specific regulation of GnRH secretion using fast-scan cyclic voltammetry to detect GnRH release in brain slices from adult male mice. Blocking endo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1458
更新日期:2015-01-01 00:00:00
abstract::Metastatic pheochromocytoma continues to be an incurable disease, and treatment with conventional cytotoxic chemotherapy offers limited efficacy. In the present study, we evaluated a novel topoisomerase I inhibitor, LMP-400, as a potential treatment for this devastating disease. We found a high expression of topoisome...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1476
更新日期:2015-11-01 00:00:00
abstract::The multifunctional cytokine, transforming growth factor-beta (TGF beta), is found in many tissues in a latent or inactive form. The nature and composition of the latent complex can vary depending on tissue type. The release of active TGF beta from its latent complex is a potentially important mechanism for regulation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4944
更新日期:1997-02-01 00:00:00
abstract::The perinatal rat exhibits low serum TSH levels in the presence of very low concentrations of T3 and T4. We had reported previously that this animal does not require endogenous TRH for TSH release. We investigated here whether the inhibitory hormone somatostatin (SS) has a role in maintaining the low TSH secretory set...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1683
更新日期:1985-10-01 00:00:00
abstract::Thyroid dysfunction during human pregnancy is closely related to serious pregnancy outcome. However, the regulation and function of thyroid hormones during early pregnancy are largely unknown. We found that type II deiodinase, an enzyme converting T4 to activated T3, is highly expressed in the mouse uterus on days 3 a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1015
更新日期:2014-07-01 00:00:00
abstract::Prolactinomas are the most prevalent functional pituitary adenomas. Dopamine D2 receptor (D2R) agonists, such as bromocriptine are the first line of therapy; however, drug intolerance/resistance to D2R agonists exists. Apart from D2R agonists, there is no established medical therapy for prolactinomas; therefore, ident...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1760
更新日期:2008-08-01 00:00:00
abstract::Consumption of a low-protein, high-carbohydrate diet induces a striking increase in circulating fibroblast growth factor-21 (FGF21), which is associated with improved cardiometabolic health and increased longevity. Increased lifespan during this dietary protein "dilution" has been explained by resource-mediated trade-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00331
更新日期:2017-10-01 00:00:00
abstract::We studied the effects of 17 beta-estradiol (E2) and PTH on alkaline phosphatase activity in SaOS-2 cells that had been passaged and grown continually in the presence (SaOS + Dex) or absence (SaOS - Dex) of 10(-8) M dexamethasone (Dex). We showed that the more differentiated SaOS + Dex cells had higher alkaline phosph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.8299560
更新日期:1994-02-01 00:00:00
abstract::The biosynthesis of various hypothalamic neuropeptides has been previously reported in anterior pituitary cells but not in intermediate lobe cells. We have recently demonstrated the occurrence of two somatostatin isoforms in the frog brain, namely somatostatin-14 (SS1) and [Pro(2),Met(13)]somatostatin-14 (SS2). In the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220406
更新日期:2002-09-01 00:00:00
abstract::Around the onset of labor, uterine sensitivity to oxytocin (OT) increases tremendously. Although this is considered to reflect OT receptor (OTR) augmentation in myometrium, neither spatial expression of OTR nor the level of the receptor message during the course of pregnancy have been investigated at the molecular lev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.2.8593830
更新日期:1996-02-01 00:00:00
abstract::It is believed that a combination of environmental factors with mutagens induces carcinomas derived from thyroid follicular cells. In this study we tried to ascertain whether a single short-term exposure to external radiation is sufficient to induce thyroid carcinomas in rats under long-term high or low dietary iodine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.7.8914
更新日期:2002-07-01 00:00:00
abstract::Neurons that produce kisspeptin play a critical role in reproduction. However, understanding the molecular physiology of kisspeptin neurons has been limited by the lack of an in vivo marker for those cells. Here, we report the development of a Kiss1-CreGFP knockin mouse, wherein the endogenous Kiss1 promoter directs t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1521
更新日期:2011-11-01 00:00:00
abstract::Circulating levels of the pancreatic beta-cell peptide hormone amylin and the gut peptide PYY[3-36] increase after nutrient ingestion. Both have been implicated as short-term signals of meal termination with anorexigenic and weight-reducing effects. However, their combined effects are unknown. We report that the combi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0898
更新日期:2007-12-01 00:00:00
abstract::It has been suggested that ewes carrying the Booroola gene (Fec(B)) consistently ovulate more follicles because they recruit more primordial follicles and/or have a lower rate of atresia. If the former is correct, the pool of follicles would be depleted sooner in Fec(B) animals. We have studied follicular dynamics and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1722
更新日期:2004-06-01 00:00:00
abstract::The estrogen-induced and -dependent Syrian hamster renal tumor is the most intensively studied model in estrogen carcinogenesis. Yet, it remains confounding that the kidney of this species behaves as an estrogen target tissue. As both reproductive and urinary systems arise from the same germinal ridge, we propose that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8355
更新日期:2001-09-01 00:00:00
abstract::A thyroid transcription factor-1 (TTF-1)-binding element in the rat TSH receptor (TSHR) promoter, between -189 and -175 basepairs (bp), is important for both thyroid-specific expression and thyroid-specific TSH/cAMP autoregulation of the TSHR. The identification of an up-stream TTF-1-binding site and its relationship ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828540
更新日期:1995-01-01 00:00:00
abstract::Two experiments tested whether serotonergic neurons participate in the acute feedback effects of estradiol on LH and PRL secretion. In the first study, ovariectomized female rats received oil vehicle or estradiol benzoate and were killed 3 h later. The turnover of serotonin [5-hydroxytryptamine (5-HT)] in microdissect...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-6-1935
更新日期:1983-12-01 00:00:00
abstract::Thyroid papillary carcinoma is the most common type of endocrine cancer. It is frequently associated with genetic alterations leading to activation of the MAPK signaling pathway. The two most frequently affected genes, BRAF and RET, are activated by either point mutation or as a result of chromosomal rearrangement. Th...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0921
更新日期:2007-03-01 00:00:00
abstract::Hypothalamic peninsulae, posteriorly continuous with the brain stem, were produced in ovariectomized rhesus monkeys by aspiration of the cerebral hemispheres and all brain structures anterior and dorsal to the optic chiasm. These preparations were sustained for 52 to 60 h postoperatively by maintaining blood gases and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-4-1264
更新日期:1977-10-01 00:00:00