Abstract:
:Lethally irradiated rhesus monkeys were treated with autologous bone marrow that had either been (1) nontreated, i.e., normal; (2) depleted of T lymphocytes with a monoclonal antibody directed against rhesus T lymphocytes; (3) fractionated with the soybean agglutinin (SBA- fraction); or (4) fractionated with SBA and further depleted of T cells by E-rosetting. There was no difference in hematologic reconstitution among the animals, but all showed a marked lowering of the T helper/T suppressor ratio during the first 10 months posttransplant and reduced capability of their peripheral blood leukocytes (PBL) to produce Ig upon stimulation with pokeweed mitogen. This subnormal ability of PBL to produce Ig, as measured by plaque-forming cells in a reverse hemolytic plaque assay, was not explained entirely by the altered T-H/T-S ratio but was correlated with the functional status of the T-H, T-S, and B lymphocytes. Isolated populations of the different lymphocyte subsets from PBL of the experimental animals were cocultured with normal cells of the appropriate subset to obtain Ig synthesis when stimulated with PWM. Animals treated with normal bone marrow showed recovery of T-H cell function after 5 months, but their T-S cells showed excessive suppressor activity that persisted for 20 months posttransplant. In contrast, those animals receiving treated marrow (mAb plus complement, or SBA) showed a much-delayed (12 months or more) return to normal T-H cell function and an earlier return of T-S cells expressing a normal level of suppressor activity. Since the SBA- fraction of marrow contains very few or no T-H cells and T cell depletion of marrow with mAb also removes these cells, it is suggested that the kinetics of immune recovery of the different lymphocyte subsets of PBL is influenced by the presence or absence of T-H cells in the marrow inocula.
journal_name
Transplantationjournal_title
Transplantationauthors
Gengozian N,Moriarty C,Good RAdoi
10.1097/00007890-199206000-00027subject
Has Abstractpub_date
1992-06-01 00:00:00pages
1313-22issue
6eissn
0041-1337issn
1534-6080journal_volume
53pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::We examined the effect of methotrexate (MTX) combined with cyclosporine (CSP) on the prevention of graft-versus-host disease (GVHD) in dogs. Ten recipients were prepared for grafting with 9 Gy of total-body irradiation and given marrow and buffy coat cells from littermate donors differing for one DLA haplotype (AA lea...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198401000-00017
更新日期:1984-01-01 00:00:00
abstract::We have previously observed that transplant recipient sera with endothelial antibodies are bound to epidermal cells, as shown by immunofluorescence on sections of skin. It was also reported that early kidney failures that occurred despite negative T cell crossmatches were associated with, and could have been predicted...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199102000-00019
更新日期:1991-02-01 00:00:00
abstract::Nuclear imaging is used to evaluate renal allografts demonstrating delayed function after transplantation. Interpretation of the nuclear scan in the context of clinical data, provides helpful information in the management of the transplant recipient. The better quality of images obtained with technetium-99m mercaptoac...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199711270-00023
更新日期:1997-11-27 00:00:00
abstract::Acute rejection (AR) remains the biggest challenge during the first year after heart transplantation despite advances in immunosuppressive therapy. The early detection and curbing of AR are crucial to the survival of transplant recipients. However, as the criterion standard for AR, endomyocardial biopsy has several li...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/TP.0000000000001780
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:The hepatic arteries (HAs) in a hepatic graft are usually reconstructed using the recipient's left or right HAs in living donor liver transplantation (LDLT). There have been no apparent selection criteria concerning which of the recipient's HAs should be used. This study retrospectively investigated whether ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/tp.0b013e3181ce77c4
更新日期:2010-04-27 00:00:00
abstract::Over the decade between 2003 and 2012, the UK has seen major changes in how organ donation and transplantation is delivered. The number of deceased organ donors has increased from 709 (12.0 per million population [pmp]) to 1,164 (18.3 pmp); this increase has been predominantly a result of an increase in donors after c...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000438215.16737.68
更新日期:2014-01-15 00:00:00
abstract:BACKGROUND:Recent evidence suggests that living kidney donors are at an increased risk of end-stage renal disease. However, predicting which donors will have renal dysfunction remains challenging, particularly among those with no clinical evidence of disease at the time of donation. Although renal biopsies are not rout...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001236
更新日期:2016-06-01 00:00:00
abstract::Recent suggestions that the rat prostate is an alymphatic, immunologically privileged site stimulated further investigation of its status using a variety of techniques. Fixation of certain organs by vascular perfusion of glutaraldehyde followed by plastic embedding avoids the distortion and stromal reorganization that...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197902000-00011
更新日期:1979-02-01 00:00:00
abstract:BACKGROUND:An increase in serum alpha-glutathione S-transferase concentration (GST) has been shown to be a more sensitive and specific marker of hepatocellular damage than equivalent increases in transaminase activities. A randomized clinical trial of 60 liver transplants in 49 patients was carried out to assess the cl...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199711270-00013
更新日期:1997-11-27 00:00:00
abstract::Thirteen renal allograft recipients (6 patients on cyclosporine/7 patients on azathioprine) participated in a short-term oral protein-loading study to determine the effect of cyclosporine on an allograft's functional reserve. The baseline glomerular filtration rate of the two groups were similar (52 +/- 18 ml/min/1.73...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199101000-00030
更新日期:1991-01-01 00:00:00
abstract::The glycosaminoglycan hyaluronan is not only involved in cellular differentiation and migration but may also play a role in several inflammatory diseases. We have previously demonstrated an increased local production of hyaluronan in chronically rejected kidneys and the correspondingly affected renal vessels. In this ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199306000-00025
更新日期:1993-06-01 00:00:00
abstract::OKT3 is considered to be effective in the prophylaxis and treatment of rejection. However, anti-OKT3 immunization is still a major side-effect. Only the IgG antiidiotypic component of the response is responsible for neutralization of OKT3 therapeutic activity by inhibiting OKT3's binding to T cells (i.e., blocking ant...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/00007890-199201000-00016
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:An artificial neural networks (ANNs) model was developed to predict 5-year graft survival of living-donor kidney transplants. Predictions from the validated ANNs were compared with Cox regression-based nomogram. METHODS:Out of 1900 patients with living-donor kidney transplant; 1581 patients were used for tr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31818b221f
更新日期:2008-11-27 00:00:00
abstract:BACKGROUND:Chronic red cell aplasia can develop in immunocompromised patients including transplant recipients infected with parvovirus B19 (PV B19). Renal involvement with PV B19 infection is not well-recognized. METHODS:We diagnosed erythroid hypoplasia associated with PV B19 infection in three renal transplant recip...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199712270-00037
更新日期:1997-12-27 00:00:00
abstract:BACKGROUND:CTLA4IgG that binds to B7 effectively inhibits the signaling of CD28/B7 pathway and induces antigen-specific T-cell unresponsiveness in vitro and in vivo. We examined whether the development of obliterative bronchiolitis in a murine heterotopic airway transplantation model is T cell dependent and whether CTL...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200003150-00012
更新日期:2000-03-15 00:00:00
abstract:BACKGROUND:Dysregulation of the coagulation system due to inflammatory responses and cross-species molecular incompatibilities represents a major obstacle to successful xenotransplantation. We hypothesized that complement inhibition mediated by transgenic expression of human CD46 in pigs might also regulate the coagula...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000746
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Liver endothelial cells (LECs) perform an essential role in important pathophysiologic functions in the liver. Establishment of a human LEC line facilitates advances in LEC research. Here, we present immortalization of human LECs using retroviral gene transfer of simian virus 40 large T antigen (SV4...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000124286.82961.7e
更新日期:2004-05-15 00:00:00
abstract::The role of TNF in the expression of GVHD and GVHD-related immunodeficiency was studied in a well-established murine GVHD model of bone marrow transplantation across minor histocompatibility barriers (B10.BR-->GBA/J) both in vitro and in vivo. Splenocytes from animals with GVHD profoundly inhibited the proliferation o...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199401001-00021
更新日期:1994-01-01 00:00:00
abstract::Methods are described for preparing large amounts of horse anti-human thymocyte globulin (ATG, ATGAM; The Upjohn Company) for clinical use. These methods have been used since 1968 to provide material for clinical trials. Characteristics of 40 lots of ATG are summarized. ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197910000-00007
更新日期:1979-10-01 00:00:00
abstract:BACKGROUND:Recently published pediatric trial of Rituximab for the treatment of CD20+ acute renal allograft rejection (AR) demonstrated transient depletion of circulating/intragraft B cells (Zarkhin et al., Am J Transplant 2008; 8: 2607). In this study, we have evaluated phenotypic definition of circulating B-cell subs...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318213df29
更新日期:2011-05-15 00:00:00
abstract:BACKGROUND:Infections and acute graft-versus-host disease (GvHD) represent major complications of allogeneic stem-cell transplantation (SCT). Dendritic cells (DCs) display an extraordinary capacity to induce innate and adaptive immune responses. Therefore, they play a crucial role in the elimination of pathogens and in...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31824fd8b4
更新日期:2012-06-27 00:00:00
abstract::Long-term graft survival is mainly influenced by early graft rejection and posttransplant graft function. The ability of both complement-dependent cytotoxicity cross-match (CDC) and flow cytometry cross-match (FCXM) to predict acute rejection episodes has been evaluated by cross-matching 40 patients who received cadav...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199702270-00012
更新日期:1997-02-27 00:00:00
abstract::The interactions of steroid hormones and human peripheral blood lymphocytes (PBLs) have been investigated following glass-wool column separation of PBLs and incubation in a serum-free medium in the presence of phytohemagglutinin (PHA). Addition of hydrocortisone or progesterone above physiological concentrations resul...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197905000-00004
更新日期:1979-05-01 00:00:00
abstract::Chronic pulmonary disease is associated with varying degrees of cardiac dysfunction. Because of the potentially predominant effect of severe lung disease on right ventricular (RV) size and function, a reliable method to assess RV mechanics before and after lung transplantation may provide information of long-term sign...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199305000-00020
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:Over 85% of US centers adhere to practice guidelines that consider morbid obesity to be a contraindication to liver transplantation (LT). The relationship of morbid obesity with LT outcomes and survival benefit in the current era is unknown. METHODS:We investigated the association of body mass index with wa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001461
更新日期:2016-12-01 00:00:00
abstract::Islets were isolated using neutral red as a dye to exclude lymphatic and acinar impurities in the transplant preparation. In a one donor-one recipient model, intraportally transplanted isogeneic islets survived indefinitely. Mean graft survival could be prolonged from 5 to 90 days, using a 3-dose rejection prophylaxis...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198604000-00003
更新日期:1986-04-01 00:00:00
abstract::Hepatic artery thrombosis is a dreadful complication of orthotopic liver transplantation. It should be suspected in cases of fulminant liver failure, delayed bile leak, or intermittent sepsis of unknown cause after liver transplantation. Accurate diagnosis is assisted by ultrasound and computerized tomography scans, b...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198512000-00019
更新日期:1985-12-01 00:00:00
abstract::The results of OKT3 use for steroid-resistant rejection rescue in adult liver transplantation were analyzed retrospectively from a single transplant center. Comparison was made with concurrent patients who had no rejection (NR) or steroid-responsive rejections (SR). The records of 290 patients who underwent 323 liver ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199301000-00017
更新日期:1993-01-01 00:00:00
abstract::Patients undergoing primary cadaveric kidney transplantation were followed for 1 year as part of a phase II, multicenter, open-label concentration-ranging trial of FK506 and cyclosporine. One hundred twenty patients were randomly assigned to a cyclosporine-based regimen or one of three FK506-based regimens designed to...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1097/00007890-199606150-00005
更新日期:1996-06-15 00:00:00
abstract:BACKGROUND:Galbeta1-4GlcNAcalpha2-6 sialyltransferase (ST6GalI) is an acute phase reactant whose release from cells can be induced by proinflammatory cytokines. Because patients with chronic renal failure have high circulating levels of proinflammatory cytokines, we hypothesized that patients on the renal transplant wa...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200003150-00022
更新日期:2000-03-15 00:00:00