Abstract:
:Newborn foreskin and adult skin keratinocytes (KTs) were cultured in 24-well plates using keratinocyte basal medium (KBM) either alone or supplemented with epidermal growth factor (EGF) or nerve growth factor (NGF), plus one of the following: insulin (INS), insulin-like growth factors (IGF)-1 or -2, transforming growth factor alpha (TGF alpha), basic fibroblast growth factor (bFGF). Culture was maintained until one group of cells reached about 30,000 cells/well, when cells were stained with crystal violet and the extracted dye used to quantify cell numbers. In some cases, cells were subjected to the hexosaminidase assay for enumeration. In KBM alone, EGF, IGF-1, IGF-2 and TGF alpha were mitogenic to newborn KTs. In addition, NGF increased the growth of adult KTs, possibly by mechanisms involving synergy with autocrine growth factors. EGF augmented the growth of newborn cells in the presence of each of the growth factors except TGF alpha, but adult cells exhibited only additive effects. In the presence of IGF-1 or IGF-2, NGF stimulated the growth of both newborn and adult cells by as much as 150% above purely additive increases in cell numbers. NGF amplifies the effects of most neurotrophic factors that are also KT mitogens and may therefore be significant in psoriatic lesions, where many of these factors are overexpressed, and in wound healing, in promoting KT growth.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Wilkinson DI,Theeuwes MJ,Farber EMdoi
10.1111/j.1600-0625.1994.tb00283.xsubject
Has Abstractpub_date
1994-10-01 00:00:00pages
239-45issue
5eissn
0906-6705issn
1600-0625journal_volume
3pub_type
杂志文章abstract::The skin forms a barrier that prevents dehydration and keeps us safe from pathogens. To ensure proper function, the skin possesses a myriad of stem cell populations that are essential for maintenance and repair upon damage. In order to protect, the skin is also an active immunological site, with abundant resident immu...
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pub_type: 评论,杂志文章
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journal_title:Experimental dermatology
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pub_type: 杂志文章,评审
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pub_type: 信件,随机对照试验
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
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journal_title:Experimental dermatology
pub_type: 信件
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journal_title:Experimental dermatology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00