Abstract：
:The denitration of a dihydropyridine derivative having two nitrate ester groups, 2-nitroxypropyl 3-nitrooxypropyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3, 5-pyridinedicarboxylate (NND), by rabbit hepatic cytosol was investigated. Sephadex G-150 chromatography of ammonium sulfate precipitate (30-60%) from the cytosol demonstrated the presence of two distinct activities (peak I and peak II) responsible for denitration of [14C]-NND. The first peak, peak I, was observed in the presence of dithiothreitol (DTT), but not in the presence of glutathione (GSH). Moreover, the denitration activity of peak I was not inhibited by S-hexyl GSH, an inhibitor of GSH S-transferase (GST), indicating that peak I possessed no GST activity. In contrast, the denitration activity of peak II, having GST activity, required GSH and was inhibited by S-hexyl GSH. These results strongly suggest that the GSH-independent enzyme system(s), in addition to GST, is responsible for denitration of nitrate esters of NND.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Ogawa N,Hirose T,Fukushima K,Suwa T,Satoh Tdoi
10.1016/s0006-2952(94)00534-6subject
Has Abstractpub_date
1995-01-18 00:00:00pages
141-6issue
2eissn
0006-2952issn
1873-2968pii
S0006-2952(94)00534-6journal_volume
49pub_type
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