当前位置： SCI文献检索 > BIOCHEMICAL PHARMACOLOGY期刊下所有文献 > Impact of a novel cardioprotective agent on the ischaemia-reperfusion-induced Akt kinase activation.

Impact of a novel cardioprotective agent on the ischaemia-reperfusion-induced Akt kinase activation.

Abstract：

:Cardioprotective effect of a free radical-scavenging compound (HO-3073) was examined during ischaemia-reperfusion (IR) in isolated heart perfusion system and its influence on the pro-survival Akt signalling pathway was addressed. Rat hearts were perfused according to the Langendorff method and subjected to a global 25-min ischaemia and 15, 45 and 90-min reperfusion either untreated or treated with HO-3073 (2, 5 and 10 microM) and/or wortmannin (100 nM, inhibitor of phosphatidylinositol-3-kinase). HO-3073 facilitated the recovery of myocardial energy metabolism as assessed by 31P NMR spectroscopy (creatine phosphate recovery in reperfusion was 76+/-5%, while in untreated hearts 32+/-4%). Functional performance of the hearts followed by a left ventricular balloon manometer was also markedly improved by HO-3073 administration (recovery of rate-pressure product related to normoxia was 47+/-3%, while in untreated hearts 12+/-3%). HO-3073 diminished the infarct size measured by TTC staining (29+/-6% as opposed to 64+/-7% in untreated ischaemia-reperfusion). HO-3073 also significantly attenuated lipid peroxidation (thiobarbituric acid reactive substances) and protein oxidation (protein carbonyl content) compared to untreated hearts. HO-3073 enhanced the ischaemia-reperfusion-triggered phosphorylation of Akt-1 (activation) and glycogen synthase kinase-3 beta (inactivation) as evidenced by Western blot analysis. Wortmannin co-administration neutralised the beneficial effects of HO-3073 on cardiac energetics, contractile function, infarct size, as well as Akt signalling. Our results first display that a radical-scavenging molecule possesses the ability to intensify the pro-survival functioning of phosphatidylinositol-3-kinase/Akt pathway, which is presumed to play an additive role in the cardioprotective properties of HO-3073.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Toth A,Kovacs K,Deres P,Halmosi R,Czopf L,Hanto K,Kalai T,Hideg K,Sumegi B,Toth K

doi

10.1016/j.bcp.2003.08.007

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

2263-72

issue

11

eissn

0006-2952

issn

1873-2968

pii

S0006295203006257

journal_volume

66

pub_type

杂志文章

相关文献

BIOCHEMICAL PHARMACOLOGY文献大全
  • Effects of the enantiomers of lansoprazole (AG-1749) on (H+ + K+)-ATPase activity in canine gastric microsomes and acid formation in isolated canine parietal cells.

    abstract：:The effects of the enantiomers of 2-[[[3-methyl-4-(2,2,2-trifluoroethoxy)-2-pyridyl]methyl]-sulfinyl ]- 1H-benzimidazole (lansoprazole, AG-1749) on acid formation in isolated canine parietal cells and (H+ + K+)-ATPase activity in canine gastric microsomes were investigated. Both the (+)-and the (-)-enantiomer of lanso...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(91)90584-r

    authors： Nagaya H,Inatomi N,Nohara A,Satoh H

    更新日期：1991-10-24 00:00:00

  • Amplification of bleomycin-induced DNA cleavage at cytosine residues 3' to GGG sequences by pyrrole triamide.

    abstract：:We investigated the amplification of bleomycin-induced DNA cleavage by synthetic triamides containing N-methylpyrrole (Py) and/or N-methylimidazole (Im), PyPyPy, PyPyIm, PyImPy, and PyImIm, using 32P-labeled DNA fragments obtained from the human c-Ha-ras-1 and p53 genes. Peplomycin, a bleomycin analog, plus Fe(II) cau...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(00)00563-3

    authors： Hiraku Y,Oikawa S,Kuroki K,Sugiyama H,Saito I,Kawanishi S

    更新日期：2001-02-01 00:00:00

  • Role of fluroacetate in the toxicity of 2-fluroethylnitrosoureas.

    abstract：:The possible role of fluroacetate in the toxicity and antitumour activity of the fluroethylnitrosoureas, BFNU and FCNU has been studied in CBA mice bearing the TLX5 lymphoma either sensitive (TLXS) or resistant (TLXRT) to nitrosoureas. Treatment of mice bearing either TLXS or TLXRT tumours with either BFNU or FCNU cau...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(85)90353-3

    authors： Tisdale MJ,Brennan RA

    更新日期：1985-09-15 00:00:00

  • Increased Na+/K(+)-pump activity and adenosine triphosphate utilization after compound 48/80-induced histamine secretion from rat mast cells.

    abstract：:The Na+/K(+)-pump activity and the utilization of adenosine triphosphate (ATP) were studied in rat peritoneal mast cells after histamine secretion induced by compound 48/80. We measured the ouabain-sensitive K(+)-uptake by a radioactive technique (86Rb+). The ATP content and the glycolytic ATP-production were measured...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(94)90299-2

    authors： Johansen T,Praetorius H

    更新日期：1994-05-18 00:00:00

  • SGS742: the first GABA(B) receptor antagonist in clinical trials.

    abstract：:The GABA(B) receptor antagonist SGS742 (CGP36742) displays pronounced cognition enhancing effects in mice, young and old rats and in Rhesus monkeys in active and passive avoidance paradigms, in an eight-arm radial maze and a Morris water maze and in a social learning task. SGS742 blocks the late inhibitory postsynapti...

    journal_title：Biochemical pharmacology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.bcp.2004.07.030

    authors： Froestl W,Gallagher M,Jenkins H,Madrid A,Melcher T,Teichman S,Mondadori CG,Pearlman R

    更新日期：2004-10-15 00:00:00

  • Metabolic alterations by clofibric acid in the formation of molecular species of phosphatidylcholine in rat liver.

    abstract：:The mechanism by which p-chlorophenoxyisobutyric acid (clofibric acid) induces striking changes in the proportion of the molecular species of phosphatidylcholine (PC) in rat liver was studied. Treatment of rats with clofibric acid strikingly increased the content of 1-palmitoyl-2-oleoyl (16:0-18:1) PC, but decreased t...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(01)00735-3

    authors： Mizuguchi H,Kudo N,Kawashima Y

    更新日期：2001-10-01 00:00:00

  • Comparative in vitro metabolism of phospho-tyrosol-indomethacin by mice, rats and humans.

    abstract：:Phospho-tyrosol-indomethacin (PTI; MPI 621), a novel anti-cancer agent, is more potent and safer than conventional indomethacin. Here, we show that PTI was extensively metabolized in vitro and in vivo. PTI was rapidly hydrolyzed by carboxylesterases to generate indomethacin as its major metabolite in the liver microso...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2013.01.031

    authors： Xie G,Zhou D,Cheng KW,Wong CC,Rigas B

    更新日期：2013-04-15 00:00:00

  • Site-specific DNA damage induced by sulfite in the presence of cobalt(II) ion. Role of sulfate radical.

    abstract：:The reactivities of sulfite (SO23-) with DNA in the presence of metal ions were investigated by a DNA sequencing technique using 32P-labeled DNA fragments obtained from human c-Ha-ras-1 protooncogene. Sulfite caused DNA damage in the presence of Co2+, Cu2+ and Mn2+, although sulfite alone or metal ion alone did not. T...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(89)90119-6

    authors： Kawanishi S,Yamamoto K,Inoue S

    更新日期：1989-10-15 00:00:00

  • The effect of diabetes on the in vivo acetylation capacity of the spontaneously diabetic, insulin-dependent BB/Edinburgh Wistar rat.

    abstract：:In contrast to previous studies using chemically-induced diabetic rats, the in vivo acetylation of sulphamethazine is increased in spontaneously diabetic, insulin-dependent BB/Edinburgh (BB/E) Wistar rats compared to non-diabetic control animals from the same colony. In both diabetic and non-diabetic rats, male animal...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(91)90540-l

    authors： Lindsay RM,Baird JD

    更新日期：1991-02-01 00:00:00

  • Measurement of affective state during chronic nicotine treatment and withdrawal by affective taste reactivity in mice: the role of endocannabinoids.

    abstract：:Despite tobacco being highly addictive, it is unclear if nicotine has significant affective properties. To address this, we studied taste reactions to gustatory stimuli, palatable sucrose and unpalatable quinine, which are believed to reflect ongoing affective state. Taste reactivity was assessed during chronic nicoti...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2009.06.017

    authors： Wing VC,Cagniard B,Murphy NP,Shoaib M

    更新日期：2009-10-01 00:00:00

  • Lidocaine inhibits priming and protein tyrosine phosphorylation of human peripheral neutrophils.

    abstract：:The addition of agents, such as tumor necrosis factor-alpha, to human peripheral neutrophils (HPPMN) induces priming, which enhances the receptor-mediated superoxide (O2-) generation and tyrosine phosphorylation of several HPPMN proteins. Lidocaine, a local anesthetic, inhibited both enhanced O2- generation and tyrosi...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(93)90299-c

    authors： Kanbara T,Tomoda MK,Sato EF,Ueda W,Manabe M

    更新日期：1993-04-22 00:00:00

  • Effect of chenodeoxycholate feeding upon the biliary output of plasma membrane enzymes in the rat.

    abstract：:In model experiments using human erythrocytes, glycochenodeoxycholate caused extensive membrane damage (as judged by release of membrane phospholipid and acetylcholinesterase and by cell lysis) at approximately 10-fold lower concentrations than glycocholate. Chenodeoxycholate feeding had no effect upon the total prote...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(84)90660-9

    authors： Rahman K,Billington D

    更新日期：1984-07-15 00:00:00

  • Inhibition of IgE-mediated release of histamine and peptide leukotriene from human basophils and mast cells by forskolin.

    abstract：:Forskolin, a diterpene compound isolated from the roots of Coleus forskohlii, activates adenylate cyclase in membranes from a variety of mammalian tissues. We found that forskolin (10(-7) to 3 X 10(-5) M) caused a concentration-related inhibition of IgE-mediated release of histamine and peptide leukotriene C4 (LTC4) f...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(87)90377-7

    authors： Marone G,Columbo M,Triggiani M,Cirillo R,Genovese A,Formisano S

    更新日期：1987-01-01 00:00:00

  • Alteration of mast cell responsiveness to adenosine by pertussis toxin.

    abstract：:Adenosine potentiates mouse bone marrow-derived mast cell mediator release by a mechanism that appears to involve cell surface adenosine receptors. In an attempt to explore possible interactions between G proteins and adenosine receptors, mast cells were incubated with activated pertussis toxin, an agent that ADP-ribo...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(88)90088-3

    authors： Marquardt DL,Walker LL

    更新日期：1988-10-15 00:00:00

  • Insulin and glucose induced changes in expression level of nucleoside transporters and adenosine transport in rat T lymphocytes.

    abstract：:Adenosine is an endogenous agent exerting potent action on the immune system including regulation of lymphocyte functioning. Impaired T lymphocyte functioning is a common feature of diabetes. The aims of this study were to examine the effects of glucose and insulin on nucleoside transporters (NT) expression level and ...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2004.06.020

    authors： Sakowicz M,Szutowicz A,Pawelczyk T

    更新日期：2004-10-01 00:00:00

  • A selective NaV1.1 activator with potential for treatment of Dravet syndrome epilepsy.

    abstract：:Dravet syndrome (DS) is a catastrophic epileptic encephalopathy characterised by childhood-onset polymorphic seizures, multiple neuropsychiatric comorbidities, and increased risk of sudden death. Heterozygous loss-of-function mutations in one allele of SCN1A, the gene encoding the voltage-gated sodium channel 1.1 (NaV...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2020.113991

    authors： Chow CY,Chin YKY,Ma L,Undheim EAB,Herzig V,King GF

    更新日期：2020-11-01 00:00:00

  • Stereoselective sulfoxidation of sulindac sulfide by flavin-containing monooxygenases. Comparison of human liver and kidney microsomes and mammalian enzymes.

    abstract：:The stereoselective sulfoxidation of the pharmacologically active metabolite of sulindac, sulindac sulfide, was characterized in human liver, kidney, and cDNA-expressed enzymes. Kinetic parameter estimates (pH = 7.4) for sulindac sulfoxide formation in human liver microsomes (N = 4) for R- and S-sulindac sulfoxide wer...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(00)00301-4

    authors： Hamman MA,Haehner-Daniels BD,Wrighton SA,Rettie AE,Hall SD

    更新日期：2000-07-01 00:00:00

  • Changes in rat hepatic microsomal mixed function oxidase activity following exposure to halothane under various oxygen concentrations.

    abstract：:This study demonstrates that the exposure of phenobarbitone-treated rats to halothane at an oxygen concentration of either 10% or 14% results in marked decreases in cytochrome P-450 content and aminopyrine demethylase activity in animals sacrificed from 1 to 48 hr post-exposure. The alterations observed in the hepatic...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(87)90182-1

    authors： Knights KM,Gourlay GK,Cousins MJ

    更新日期：1987-03-15 00:00:00

  • 7-Ketocholesterol induces P-glycoprotein through PI3K/mTOR signaling in hepatoma cells.

    abstract：:7-Ketocholesterol (7-KC) is found at an elevated level in patients with cancer and chronic liver disease. The up-regulation of an efflux pump, P-glycoprotein (P-gp) leads to drug resistance. To elucidate the effect of 7-KC on P-gp, P-gp function and expression were investigated in hepatoma cell lines Huh-7 and HepG2 a...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2013.06.006

    authors： Wang SF,Chou YC,Mazumder N,Kao FJ,Nagy LD,Guengerich FP,Huang C,Lee HC,Lai PS,Ueng YF

    更新日期：2013-08-15 00:00:00

  • Fluctuation of fetal rat hepatic histidine decarboxylase activity through the glucocorticoid-ACTH system.

    abstract：:Our studies suggest that the fluctuation of HDC activity in fetal liver in late gestation is regulated by the plasma glucocorticoid level through the pituitary-adrenal system. Taken together, these results support the conclusion that glucocorticoid promotes a rapid increase in HDC synthesis in fetal liver histamine-fo...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(91)90092-j

    authors： Ohmori E,Imanishi N,Ohgoh M,Fukui T,Ichikawa A

    更新日期：1991-03-01 00:00:00

  • A sensitive new method for clinically monitoring cytarabine concentrations at the DNA level in leukemic cells.

    abstract：:Cytarabine (ara-C), a major antileukemic agent, is phosphorylated in the cell to cytarabine triphosphate (ara-CTP), which is then partly incorporated into DNA. The drug incorporation into DNA poisons the extending primer against further incorporation of deoxyribonucleotides including dCTP, ultimately inhibiting DNA sy...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2005.03.013

    authors： Yamauchi T,Ueda T

    更新日期：2005-06-15 00:00:00

  • Pharmacogenetics of human 3'-phosphoadenosine 5'-phosphosulfate synthetase 1 (PAPSS1): gene resequencing, sequence variation, and functional genomics.

    abstract：:3'-Phosphoadenosine 5'-phosphosulfate (PAPS) is the high-energy "sulfate donor" for reactions catalyzed by sulfotransferase (SULT) enzymes. The strict requirement of SULTs for PAPS suggests that PAPS synthesis might influence the rate of sulfate conjugation. In humans, PAPS is synthesized from ATP and SO(4)(2-) by two...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(03)00104-7

    authors： Xu ZH,Thomae BA,Eckloff BW,Wieben ED,Weinshilboum RM

    更新日期：2003-06-01 00:00:00

  • Changes in the biliary excretion of organic anions following exhaustive exercise in rats.

    abstract：:The effect of exhaustive exercise on the hepatobiliary transport of organic anions was investigated in rats. Animals were run on a rodent treadmill at 24 m/min up a 12% grade (152 +/- 15 min). Exercise resulted in significant hypoglycaemia (-46%) and increased plasma levels of lactate (+12%), together with a marked re...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(90)90094-2

    authors： Villa JG,Collado PS,Almar MM,Gonzalez J

    更新日期：1990-12-01 00:00:00

  • Specific, high-affinity bradykinin binding by purified porcine kidney post-proline cleaving enzyme.

    abstract：:Post-proline cleaving enzyme (PPCE) was purified from porcine kidney cytosol. The purified enzyme bound [125I-Tyr5]-bradykinin but neither [125I-Tyr1]-kallidin nor [125I-Tyr8]-bradykinin. Scatchard analysis of the data was consistent with a single class of binding sites with a Kassoc = 1.3 +/- 0.1 X 10(8) M-1. The opt...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(87)90380-7

    authors： Odya CE,Dally RD,Georgiadis KE

    更新日期：1987-01-01 00:00:00

  • Comparative affinity of steroidal and non-steroidal antioestrogens, cholesterol derivatives and compounds with a dialkylamino side chain for the rat liver antioestrogen binding site.

    abstract：:Steroidal and non-steroidal antioestrogens, steroidal compounds with (disubstituted) dialkyl amino side chain, cholesterol derivatives, histaminic and (anti)-progestational compounds were tested for their ability to compete with [3H]tamoxifen for the specific antioestrogen binding site (AEBS) in the post-mitochondrial...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(92)90138-9

    authors： van den Koedijk CD,Vis van Heemst C,Elsendoorn GM,Thijssen JH,Blankenstein MA

    更新日期：1992-06-23 00:00:00

  • Behavioural and neurochemical interactions between chronic reserpine and chronic antidepressants. A possible model for the detection of atypical antidepressants.

    abstract：:Chronic treatment with a low dose of reserpine (0.1 mg/kg) caused rats to become hyperactive in the "open field" apparatus. When mianserin (5 mg/kg) or the selective serotonin uptake inhibitor ORG. 6582 (5 mg/kg) was chronically administered in combination with reserpine, the hyperactivity was attenuated. Both antidep...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(83)90329-5

    authors： Jancsár SM,Leonard BE

    更新日期：1983-05-15 00:00:00

  • Intervention in free radical mediated hepatotoxicity and lipid peroxidation by indole-3-carbinol.

    abstract：:The cytoprotective effect of the natural dietary constituent indole-3-carbinol (I-3-C) on carbon tetrachloride (CCl4) mediated hepatotoxicity in mice was examined. I-3-C pretreatment by gavage 1 hr prior to intraperitoneal injection of CCl4 produced a 63% decrease in CCl4-mediated centrolobular necrosis and a related ...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/0006-2952(88)90737-x

    authors： Shertzer HG,Berger ML,Tabor MW

    更新日期：1988-01-15 00:00:00

  • Comparison of the effects of bupropion and nicotine on locomotor activation and dopamine release in vivo.

    abstract：:Bupropion is an atypical anti-depressant that is approved for smoking cessation. In addition to inhibiting dopamine reuptake, bupropion has been reported to block nicotinic acetylcholine receptors in vitro, and this action might contribute to its efficacy for smoking cessation. In this study we investigated if nicotin...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/j.bcp.2007.06.025

    authors： Sidhpura N,Redfern P,Rowley H,Heal D,Wonnacott S

    更新日期：2007-10-15 00:00:00

  • Correlation of a unique 220-kDa protein with vitamin D sensitivity in glioma cells.

    abstract：:We have investigated the antitumor and apoptotic effects of 1, 25-dihydroxyvitamin D(3) (VD(3)) in glioma cell lines and in primary cultures derived from surgical specimens from patients. Our results showed that certain glioma cells underwent apoptosis, whereas others were resistant. In an attempt to search for parame...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(00)00438-x

    authors： Zou J,Landy H,Feun L,Xu R,Lampidis T,Wu CJ,Furst AJ,Savaraj N

    更新日期：2000-11-01 00:00:00

  • Concordance between enzyme activity and genotype of glutathione S-transferase theta (GSTT1).

    abstract：:Blood samples from 140 healthy German volunteers were used to further characterize the genetic polymorphism of the human theta class glutathione S-transferase 1 (GSTT1). For measurements of GSTT1 activity, hemolysates were incubated in vitro with different concentrations of dichloromethane. The resulting enzymatically...

    journal_title：Biochemical pharmacology

    pub_type： 杂志文章

    doi：10.1016/s0006-2952(98)00191-9

    authors： Bruhn C,Brockmöller J,Kerb R,Roots I,Borchert HH

    更新日期：1998-11-01 00:00:00