The A-state of barnase.

abstract：:Cellular toxicity resulting from nucleation-dependent polymerization of amyloid beta-peptide (Abeta) is considered to be a major and possibly the primary component of Alzheimer's disease (AD). Inhibition of Abeta polymerization has thus been identified as a target for the development of therapeutic agents for the trea...

journal_title：Biochemistry

authors： Findeis MA,Musso GM,Arico-Muendel CC,Benjamin HW,Hundal AM,Lee JJ,Chin J,Kelley M,Wakefield J,Hayward NJ,Molineaux SM

更新日期：1999-05-25 00:00:00

abstract：:CD6 is a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). This family includes many cell surface proteins whose three-dimensional structures and functions are presently not well understood. The extracellular region of CD6 includes 3 SRCR domains. The membrane proximal SRCR domain specifical...

journal_title：Biochemistry

authors： Bodian DL,Skonier JE,Bowen MA,Neubauer M,Siadak AW,Aruffo A,Bajorath J

更新日期：1997-03-04 00:00:00

abstract：:The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...

journal_title：Biochemistry

authors： Bentrop D,Bertini I,Cremonini MA,Forsén S,Luchinat C,Malmendal A

更新日期：1997-09-30 00:00:00

abstract：:Nogo-66 receptor (NgR1) is a leucine-rich repeat (LRR) protein that forms part of a signaling complex modulating axon regeneration. Previous studies have shown that the entire LRR region of NgR1, including the C-terminal cap of the LRR, LRRCT, is needed for ligand binding, and that the adjacent C-terminal region (CT s...

journal_title：Biochemistry

authors： Wen D,Wildes CP,Silvian L,Walus L,Mi S,Lee DH,Meier W,Pepinsky RB

更新日期：2005-12-20 00:00:00

abstract：:The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...

journal_title：Biochemistry

authors： Yanagisawa S,Sato K,Kikuchi M,Kohzuma T,Dennison C

更新日期：2003-06-10 00:00:00

abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...

journal_title：Biochemistry

authors： Sulkes M,Lewis A,Marcus MA

更新日期：1978-10-31 00:00:00

abstract：:SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural differences...

journal_title：Biochemistry

authors： Smola MJ,Calabrese JM,Weeks KM

更新日期：2015-11-24 00:00:00

abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...

journal_title：Biochemistry

authors： Sato M,Kanamori T,Kamo N,Demura M,Nitta K

更新日期：2002-02-19 00:00:00

abstract：:Phage T4 gene 32 protein (gp32) is a zinc metalloprotein which binds cooperatively and preferentially to single-stranded nucleic acids and functions as a replication and recombination accessory protein. Zn(II) coordination by gp32 employs a His-Cys3 metal ligand donor set derived from the His64-X12-Cys77-X9-Cys87-X2-C...

journal_title：Biochemistry

authors： Guo J,Giedroc DP

更新日期：1997-01-28 00:00:00

abstract：:Human P-glycoprotein (Pgp), a plasma membrane protein that confers multidrug resistance, functions as an ATP-dependent drug efflux pump. Pgp contains two ATP binding/utilization sites and exhibits ATPase activity that is stimulated in the presence of substrates and modulating agents. The mechanism of coupling of ATP h...

journal_title：Biochemistry

authors： Ramachandra M,Ambudkar SV,Chen D,Hrycyna CA,Dey S,Gottesman MM,Pastan I

更新日期：1998-04-07 00:00:00

abstract：:The alpha-helix [Pauling, L., Corey, R. B., & Branson, H. R. (1951) Proc. Natl. Acad. Sci. U.S.A. 37, 205-211] is a common motif in both proteins and peptides. Despite intense investigation, predictive understanding of helices is still lacking. A recent hypothesis [Presta, L. G., & Rose, G. D. (1988) Science 240, 1632...

journal_title：Biochemistry

authors： Harper ET,Rose GD

更新日期：1993-08-03 00:00:00

abstract：:Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...

journal_title：Biochemistry

authors： Nguyen TT,Fedorov AA,Williams L,Fedorov EV,Li Y,Xu C,Almo SC,Raushel FM

更新日期：2009-09-22 00:00:00

abstract：:Nitrobenzamido ligands linked to the DNA intercalator 9-aminoacridine via poly(methylene) chains induce single-strand nicks in DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than or equal to 300 nm). Optimal photocleavage activity was found for the reagent 9-[[6-(4-nitrobenzamido)hexyl]ami...

journal_title：Biochemistry

authors： Nielsen PE,Jeppesen C,Egholm M,Buchardt O

更新日期：1988-08-23 00:00:00

abstract：:The folding of the peptide chain of the bovine heart oxoglutarate carrier in the inner mitochondrial membrane and in the membrane of reconstituted proteoliposomes has been investigated by enzymatic and immunochemical approaches using proteinase K and polyclonal site-directed antibodies, respectively. Two peptides corr...

journal_title：Biochemistry

authors： Bisaccia F,Capobianco L,Brandolin G,Palmieri F

更新日期：1994-03-29 00:00:00

abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...

journal_title：Biochemistry

authors： Bradley M,Bücheler US,Walsh CT

更新日期：1991-06-25 00:00:00

abstract：:Factor XIII is the terminal enzyme of the clotting cascade. A cDNA sequence encoding human placental factor XIII was expressed in Saccharomyces cerevisiae with the yeast ADH2-4c promoter. Expression levels were a strong function of the noncoding flanking DNA content of the construction. When the terminal 3'-flanking n...

journal_title：Biochemistry

authors： Bishop PD,Teller DC,Smith RA,Lasser GW,Gilbert T,Seale RL

更新日期：1990-02-20 00:00:00

abstract：:Bovine brain adenylate cyclase was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium cholate, sodium deoxycholate, or these detergents plus (NH4)2SO4. The specific activity of the extract obtained with 13 mM CHAPS alone was several times those of the other detergent extracts wi...

journal_title：Biochemistry

authors： Bitonti AJ,Moss J,Hjelmeland L,Vaughan M

更新日期：1982-07-20 00:00:00

abstract：:A 37 kDa protein that binds to diadenosine tetraphosphate (Ap4A) was purified from human HeLa cells and identified as uracil DNA glycosylase/glyceraldehyde-3-phosphate dehydrogenase (UDG/GAPDH). Utilizing photoaffinity labeling with [alpha-32P]8N3-Ap4A, an Ap4A binding protein of 37 kDa was identified from HeLa cell n...

journal_title：Biochemistry

authors： Baxi MD,Vishwanatha JK

更新日期：1995-08-01 00:00:00

abstract：:A series of sulfonate esters that are attached to a noncationic minor-groove-binding N-methylpyrrole dipeptide (Lex) related to netrospin have been synthesized. The compounds prepared differ in two respects: (1) the length [(CH2)2 vs (CH2)8] of the tether between the DNA affinity binding portion of the molecule and th...

journal_title：Biochemistry

authors： Zhang Y,Chen FX,Mehta P,Gold B

更新日期：1993-08-10 00:00:00

abstract：:The rapid phosphorylation and dephosphorylation of a variety of proteins downstream of the endothelin receptors A and B was investigated following stimulation of human lung fibroblasts with endothelin-1. Changes in the phosphorylation of proteins involved in the cell cycle, cytoskeleton, membrane channels, transcripti...

journal_title：Biochemistry

authors： Stannard C,Soskic V,Godovac-Zimmermann J

更新日期：2003-12-02 00:00:00

abstract：:The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...

journal_title：Biochemistry

authors： Mizushina Y,Ohkubo T,Sugawara F,Sakaguchi K

更新日期：2000-10-17 00:00:00

abstract：:Structural properties of the isolated extrinsic regulatory 33 kDa protein of the water-oxidizing complex were analyzed at different pH values. It was found that (a) titrations of the buffer capacity reveal a characteristic hysteresis effect that is unique for the 33 kDa subunit and is not observed for the other extrin...

journal_title：Biochemistry

authors： Shutova T,Irrgang KD,Shubin V,Klimov VV,Renger G

更新日期：1997-05-27 00:00:00

abstract：:We have studied the effects of site-directed mutations in Escherichia coli nitrate reductase A (NarGHI) on heme reduction by a menaquinol analogue (menadiol) using the stopped-flow method. For NarGHI(H66Y) and NarGHI(H187Y), both lacking heme b(L) but having heme b(H), the heme reduction by menadiol is abolished. For ...

journal_title：Biochemistry

authors： Zhao Z,Rothery RA,Weiner JH

更新日期：2003-12-09 00:00:00

abstract：:Apolipoprotein AIV is one of the principal apolipoproteins synthesized by the rat small intestine. We have cloned a cDNA encoding a portion of preapolipoprotein AIV mRNA. A kinetically fractionated cDNA probe highly enriched for the abundant intestinal mucosal mRNA sequences was used to screen a library of recombinant...

journal_title：Biochemistry

authors： Gordon JI,Smith DP,Alpers DH,Strauss AW

更新日期：1982-10-26 00:00:00

abstract：:A mechanism by which the Klenow fragment of DNA polymerase I monitors the geometry of the base pairs may involve hydrogen bonds between the polymerase and the minor groove of the nascent base pair. The involvement of the 3-position of guanine in the template strand was examined by synthesizing oligodeoxynucleotides co...

journal_title：Biochemistry

authors： Spratt TE

更新日期：1997-10-28 00:00:00

abstract：:2-Cys peroxiredoxins (Prxs) are a large and diverse family of peroxidases which, in addition to their antioxidant functions, regulate cell signaling pathways, apoptosis, and differentiation. These enzymes are obligate homodimers (alpha(2)), utilizing a unique intermolecular redox-active disulfide center for the reduct...

journal_title：Biochemistry

authors： Wood ZA,Poole LB,Hantgan RR,Karplus PA

更新日期：2002-04-30 00:00:00

abstract：:The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

journal_title：Biochemistry

authors： Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

更新日期：2010-01-26 00:00:00

abstract：:The effects of urea on apomyoglobin solubility have been investigated. Apomyoglobin precipitation was found to be a thermodynamically reversible process independent of the pathway of aggregation. A liquid-solid phase diagram was constructed for the precipitation of apomyoglobin as a function of urea and protein concen...

journal_title：Biochemistry

authors： De Young LR,Dill KA,Fink AL

更新日期：1993-04-20 00:00:00

abstract：:The heme binding sites of rabbit histidine-rich glycoprotein (HRG), 94 kDa, were studied with rose bengal (RB), a fluorescein derivative that associates with histidine residues. Difference absorbance spectra indicate that HRG binds RB at two thermodynamically preferred sites (Kd approximately 2 microM) that are spectr...

journal_title：Biochemistry

authors： Burch MK,Morgan WT

更新日期：1985-10-08 00:00:00

abstract：:Inactivation of rabbit lung angiotensin converting enzyme (ACE) by 1-fluoro-2,4-dinitrobenzene (Dnp-F) has been shown to be due primarily to the modification of a tyrosine residue [Bünning, P., Kleeman, S.G., & Riordan, J.F. (1990) Biochemistry (preceding paper in this issue)]. Rabbit testicular ACE is also inactivate...

journal_title：Biochemistry

authors： Chen YN,Riordan JF

更新日期：1990-11-20 00:00:00