Abstract：
:Male pronucleus formation involves sperm nucleus decondensation and sperm chromatin remodeling. In sea urchins, male pronucleus decondensation was shown to be modulated by protein kinase C and a cdc2-like kinase sensitive to olomoucine in vitro assays. It was further demonstrated that olomoucine blocks SpH2B and SpH1 phosphorylation. These phosphorylations were postulated to participate in the initial steps of male chromatin remodeling during male pronucleus formation. At final steps of male chromatin remodeling, all sperm histones (SpH) disappear from male chromatin and are subsequently degraded by a cysteine protease. As a result of this remodeling, the SpH are replaced by maternal histone variants (CS). To define if sperm nucleus decondensation is coupled with sperm chromatin remodeling, we have followed the loss of SpH in zygotes treated with olomoucine. SpH degradation was followed with anti-SpH antibodies that had no cross-reactivity with CS histone variants. We found that olomoucine blocks SpH1 and SpH2B phosphorylation and inhibits male pronucleus decondensation in vivo. Interestingly, the normal schedule of SpH degradation remains unaltered in the presence of olomoucine. Taken together these results, it was concluded that male nucleus decondensation is uncoupled from the degradation of SpH associated to male chromatin remodeling. From these results, it also emerges that the phosphorylation of SpH2B and SpH1 is not required for the degradation of the SpH that is concurrent to male chromatin remodeling.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Monardes A,Iribarren C,Morin V,Bustos P,Puchi M,Imschenetzky Mdoi
10.1002/jcb.20583subject
Has Abstractpub_date
2005-10-01 00:00:00pages
235-41issue
2eissn
0730-2312issn
1097-4644journal_volume
96pub_type
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更新日期：2006-05-01 00:00:00
abstract：:Paeoniflorin (PF), one of the main effective ingredients from the root of Paeonia lactiflora Pall., was reported to possess antitumor, anti-inflammatory, and antiallergic properties. However, the roles of PF in activated human mast cell line, HMC-1 cells, have not yet been elucidated. Thus, the aim of this study was t...
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pub_type： 杂志文章
doi：10.1002/jcb.27135
更新日期：2018-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
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journal_title：Journal of cellular biochemistry
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doi：10.1002/jcb.24857
更新日期：2014-11-01 00:00:00
abstract：:Fourteen stable subclones derived from the murine chondrogenic cell line MC615 were established and characterised regarding their differentiation stages and responsivity to BMP2. Based on their gene expression profiles which revealed remarkable variances in Col2a1 and Col10a1 expression, subclones could be grouped int...
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更新日期：2009-10-15 00:00:00
abstract：:The four-subunit protease complex γ-secretase cleaves many single-pass transmembrane (TM) substrates, including Notch and β-amyloid precursor protein to generate amyloid-β (Aβ), central to Alzheimer's disease. Two of the subunits anterior pharynx-defective 1 (APH-1) and presenilin (PS) exist in two homologous forms AP...
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更新日期：2021-01-01 00:00:00
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journal_title：Journal of cellular biochemistry
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更新日期：2018-01-01 00:00:00
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doi：10.1002/jcb.28365
更新日期：2019-06-01 00:00:00
abstract：:Ovarian cancer (OC) is a lethal disease diagnosed at advanced stages due to the lack of specific biomarkers. Tyrosine receptor kinase B (TrkB), which has recently been found to be related to OC progression, represents a promising potential biomarker for OC diagnosis and prognosis. The discovery of circulating exosomes...
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doi：10.1002/jcb.10253
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doi：10.1002/(sici)1097-4644(20000101)76:1<44::aid-jcb6
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更新日期：2016-08-01 00:00:00
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