Abstract:
:Microdialysis is a valuable tool to measure tissue responses. We hypothesized that skeletal muscle metabolism can be modulated by microdialysis applied drugs which alter cytosolic calcium concentration. With approval of the local animal care committee, the hind limbs of sacrificed male Sprague Dawley rats were perfused either with Ringer's solution or with dantrolene 1 microM at 30 ml hr(-1) and 21 degrees. Microdialysis probes in both hind limbs were perfused at 1 microl min(-1) either with sorbitol 80 mM, calcium 20 mM, 40 mM, 80 mM, caffeine 40 mM, 80 mM, and halothane 10 vol% respectively, and at the contralateral adductor muscle with Ringer as control. Lactate was measured spectrophotometrically in the dialysate at 15 min. intervals. Lactate levels as measured by intramuscular microdialysis were not influenced by intramuscular application of sorbitol 80 mM compared to control measurements with Ringer's solution. Local application of calcium 20 mM, 40 mM, 80 mM, caffeine 40 mM, 80 mM, and halothane 10 vol% via microdialysis increased lactate concentrations, while organ perfusion by dantrolene 1 microM reduced the caffeine-induced lactate increase. Modulation of intramuscular lactate metabolism by exogenous compounds via microdialysis probes generates new insights in skeletal muscle metabolism.
journal_name
Basic Clin Pharmacol Toxicoljournal_title
Basic & clinical pharmacology & toxicologyauthors
Schuster F,Tas P,Müller R,Roewer N,Anetseder Mdoi
10.1111/j.1742-7843.2006.pto_250.xsubject
Has Abstractpub_date
2006-04-01 00:00:00pages
372-6issue
4eissn
1742-7835issn
1742-7843pii
PTOpto_250journal_volume
98pub_type
杂志文章abstract::Opioids alter resting state brain oscillations by multiple and complex factors, which are still to be elucidated. To increase our knowledge, multi-channel electroencephalography (EEG) was subjected to multivariate pattern analysis (MVPA), to identify the most descriptive frequency bands and scalp locations altered by ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,随机对照试验
doi:10.1111/bcpt.12330
更新日期:2015-05-01 00:00:00
abstract::Nicotine-methadone interactions have been studied in human beings and in various experimental settings regarding addiction, reward and pain. Most methadone maintenance treatment patients are smokers, and methadone administration has been shown to increase cigarette smoking. Previous in vitro studies have shown that me...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12317
更新日期:2015-04-01 00:00:00
abstract::Pain involves responses in which both peripheral and central mechanisms contribute to the generation of pain. Pre-clinical laboratory data have supported that a topical formulation of combined diclofenac and methadone (Diclometh) may alleviate local pain, and potentially, the side effect profile should be low. We hypo...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,随机对照试验
doi:10.1111/bcpt.12993
更新日期:2018-08-01 00:00:00
abstract::Several investigations demonstrate that neurotoxins isolated from venoms of spiders and wasps may exert specific and selective activity on structures of the mammalian CNS. In the present work we examine the neurological effects of the low molecular weight compounds of the denatured venom of the neotropical social wasp...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2005.pto_137.x
更新日期:2005-11-01 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is a frequent condition in obese patients that may progress to end-stage liver disease. This study was designed to evaluate the modulation of this condition by use of quercetin (Q), a flavonoid largely found in vegetable foods, with known anti-inflammatory and antioxidant propertie...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,收录出版
doi:10.1111/bcpt.12049
更新日期:2013-06-01 00:00:00
abstract::Several single nucleotide variations (SNVs) affect carboxylesterase 1 (CES1) activity, but the effects of genetic variants on CES1 gene expression have not been systematically investigated. Therefore, our aim was to investigate effects of genetic variants on CES1 gene expression in two independent whole blood sample c...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12916
更新日期:2018-03-01 00:00:00
abstract::δ-Containing GABAA receptors are located extrasynaptically and mediate tonic inhibition. Their involvement in brain physiology positions them as interesting drug targets. There is thus a continued interest in establishing reliable recombinant expression systems for δ-containing GABAA receptors. Inconveniently, the rec...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12778
更新日期:2017-08-01 00:00:00
abstract::Chemokines are chemotactic cytokines whose involvement in nociceptive processing is being increasingly recognized. Based on the previous description of the involvement of CC chemokine receptor type 1 (CCR1) in pathological pain, we have assessed the participation of CCR1 and its endogenous ligands CCL3 and CCL5 in hyp...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12543
更新日期:2016-07-01 00:00:00
abstract::Endotoxin-induced sepsis in pigs is a recognized experimental model for the study of human septic shock. Generally, pigs are brought into general anaesthesia before sepsis is induced. It is our experience that drug dosages of propofol and fentanyl need to be reduced during endotoxin-induced sepsis, in order to prevent...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2007.00099.x
更新日期:2007-09-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) and MSC-derived small extracellular vesicles (sEVs) are promising candidates for cell-based and cell-free regenerative medicine, respectively. By virtue of their multiple lineage differentiation capacity, MSCs have been implicated as an ideal tool for bone and cartilage regeneration. Howe...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13478
更新日期:2021-01-01 00:00:00
abstract::The currently recommended first-line treatments of erectile dysfunction (ED), phosphodiesterase type 5 inhibitors (PDE5i), for example sildenafil, are efficacious in many patients with ED of vascular origin, but this therapy is insufficient in approximately 30-40% of men with ED where there is also a neuronal affectio...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/bcpt.12653
更新日期:2016-10-01 00:00:00
abstract::In this MiniReview, we provide general considerations for the planning and conduct of pharmacoepidemiological studies of associations between drug use and cancer development. We address data sources, study design, assessment of drug exposure, ascertainment of cancer outcomes, confounder adjustment and future perspecti...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/bcpt.12946
更新日期:2018-05-01 00:00:00
abstract::Vascular networks are dynamic structures, adapting to changing conditions by structural remodelling of vessel diameters and by growth of new vessels and regression of existing vessels. The vast number of blood vessels in the circulatory system, more than 10⁹, implies that vessels' arrangement and structure are not und...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/j.1742-7843.2011.00815.x
更新日期:2012-01-01 00:00:00
abstract::Epidemiological data have shown that methamphetamine (MA) abuse significantly increases the risk of developing pulmonary arterial hypertension (PAH). To investigate whether MA could induce PAH and its possible mechanism, rats were exposed daily to MA for 5 weeks in the absence or presence of fluoxetine. The results sh...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2012.00933.x
更新日期:2013-02-01 00:00:00
abstract::The reactivating and therapeutic efficacy of three original bispyridinium oximes (K727, K733 and K203) and one currently available oxime (trimedoxime) was evaluated in tabun-poisoned rats and mice. The oxime-induced reactivation of tabun-inhibited acetylcholinesterase was measured in diaphragm and brain of tabun-poiso...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12327
更新日期:2015-04-01 00:00:00
abstract::Hypotensive effects of oral sodium nitrite have been reported as alternative sources of nitric oxide (NO) formation in animals and human beings. Reductions in NO bioavailability were observed in lead-induced hypertension. However, no previous study has examined whether a single daily dose of sodium nitrite prevents th...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12480
更新日期:2016-03-01 00:00:00
abstract::Toxic epidermal necrolysis (TEN) is a life-threatening mucocutaneous disease with high mortality. Dithiocarbamates (DTC) are organosulphur compounds widely used in agriculture, industry and households. We report a case of TEN after exposure to mancozeb in fungicide. A 48-year-old 75 kg b.w. man was admitted with fever...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12430
更新日期:2016-01-01 00:00:00
abstract::Copper deficiency had been suggested to link between fructose-enriched diet (FED) and the development of non-alcoholic fatty liver disease (NAFLD). In this study, we characterized changes in hepatic copper concentrations and hepatic oxidative milieu, in rats with the metabolic syndrome and NAFLD as a result of FED wit...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12283
更新日期:2014-12-01 00:00:00
abstract::Simvastatin (SV), a HMG-CoA reductase inhibitor, is widely used for the treatment of hyperlipidaemia. The objectives of the present study were to develop a population pharmacokinetic-pharmacodynamic (PK-PD) model for simvastatin and to evaluate its usefulness in predicting the dose-response relationship of simvastatin...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2011.00700.x
更新日期:2011-09-01 00:00:00
abstract::Cisplatin and other platinum complexes are important chemotherapeutic agents and useful in the treatment for several cancers such as prostate, ovarian and testis. However, severe side effects including reproductive toxicity of cisplatin and other platinum complex cause limitations in their clinical usage. In this cont...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2011.00737.x
更新日期:2011-11-01 00:00:00
abstract::Cell damage and spatial localization deficits are often reported as long-term consequences of pilocarpine-induced status epilepticus. In this study, we investigated the neuroprotective effects of repeated drug administration after long-lasting status epilepticus. Groups of six to eight Wistar rats received microinject...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2009.00403.x
更新日期:2009-06-01 00:00:00
abstract::The aim of the present study was to investigate the effect of intraperitoneal administration of caffeic acid (0.5, 1, 2, 4 or 8 mg/kg) on elevated plus-maze and open field tasks in rats and its possible neuroprotection/neurotoxicity using the comet assay. Caffeic acid at 1 mg/kg increased the number of entries and the...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2006.pto_533.x
更新日期:2006-11-01 00:00:00
abstract::In order to perform relevant in vivo pharmacological investigations in drug discovery within dermatology it is fundamental to master or have access to relevant skin disease models that makes it possible to identify and validate targets and to screen and discover drugs in vivo. There is a strong need for highly predict...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/j.1742-7843.2006.pto_298.x
更新日期:2006-08-01 00:00:00
abstract::The cannabinoid 1 receptor antagonist AM 251 is known to block the inhibitory effects of endocannabinoids and synthetic cannabinoid agonists on transmitter release through an action at presynaptic cannabinoid 1 receptors in brain. We examined the ability of AM 251 to inhibit sodium channel-dependent functions and the ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2004.pto940204.x
更新日期:2004-02-01 00:00:00
abstract::The OECD GD 129 BALB/c 3T3 neutral red uptake (NRU) assay is a standardized test method for estimating starting dose for an acute oral systemic toxicity test in rodents. Mouse BALB/c 3T3 fibroblasts are the most commonly used cells in the NRU assay. We have previously transferred and validated BALB/c 3T3 NRU assay in ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12790
更新日期:2017-09-01 00:00:00
abstract::Since the 1970s, we have known that aspirin can reduce the risk of pre-eclampsia. However, the underlying mechanisms explaining this risk reduction are poorly understood. Both cyclooxygenase (COX)-1- and COX-2-dependent effects might be involved. As a consequence of this knowledge hiatus, the optimal dose and timing o...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/bcpt.13308
更新日期:2020-08-01 00:00:00
abstract::To study the potential interactions of naringin (NAR), talinolol (TAL) and protein-energy undernutrition (PEU) in the absorption process of saquinavir (SQV), perfusion experiments were performed in the small intestine of rats at different SQV concentrations. The results obtained demonstrated that SQV intestinal absorp...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2011.00714.x
更新日期:2011-10-01 00:00:00
abstract::The present in vivo study investigated the genotoxicity of four dental resin monomers: triethyleneglycoldimethacrylate (TEGDMA), hydroxyethylmethacrylate (HEMA), urethanedimethacrylate (UDMA) and bisphenol A-glycidylmethacrylate (BisGMA). The Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster w...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2009.00479.x
更新日期:2010-02-01 00:00:00
abstract::Alcohol consumption increases apoptosis of hepatocytes. Death of hepatocytes is a characteristic feature of chronic liver disease for various causes. Bee venom (Apis mellifera) has been traditionally used for the treatment of various chronic diseases, such as chronic inflammatory arthritis and chronic liver disease. H...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2010.00549.x
更新日期:2010-07-01 00:00:00
abstract::This study investigated the effects of single nucleotide polymorphisms (SNPs) in xenobiotic and steroid hormone-metabolizing genes in relation to breast cancer risk and explored possible effect modifications on persistent organic pollutants (POPs) and breast cancer associations. The study also assessed effects of Gree...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13002
更新日期:2018-09-01 00:00:00