Abstract:
:1-Aminocyclopropane-1-carboxylate (ACC) synthase, a key enzyme in ethylene biosynthesis, was isolated and partially purified from apple (Malus sylvestris Mill.) fruits. Unlike ACC synthase isolated from other sources, apple ACC synthase is associated with the pellet fraction and can be solubilized in active form with Triton X-100. Following five purification steps, the solubilized enzyme was purified over 5000-fold to a specific activity of 100 micromoles per milligram protein per hour, and its purity was estimated to be 20 to 30%. Using this preparation, specific monoclonal antibodies were raised. Monoclonal antibodies against ACC synthase immunoglobulin were coupled to protein-A agarose to make an immunoaffinity column, which effectively purified the enzyme from a relatively crude enzyme preparation (100 units per milligram protein). As with the tomato enzyme, apple ACC synthase was inactivated and radiolabeled by its substrate S-adenosyl-l-methionine. Apple ACC synthase was identified to be a 48-kilodalton protein based on the observation that it was specifically bound to immunoaffinity column and it was specifically radiolabeled by its substrate S-adenosyl-l-methionine.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Yip WK,Dong JG,Yang SFdoi
10.1104/pp.95.1.251subject
Has Abstractpub_date
1991-01-01 00:00:00pages
251-7issue
1eissn
0032-0889issn
1532-2548journal_volume
95pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The low temperature exotherms (LTE) of 1-year-old twigs of Haralson apple (Malus pumila Mill.), shagbark hickory (Carya ovata [Mill.] K. Koch), green ash (Fraxinus pennsylvanica Marsh), honey locust (Gleditsia triacanthos L.), American chestnut (Castanea dentata [Marsh] Borkh.), and red oak (Quercus rubra L.) were det...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.1.40
更新日期:1980-07-01 00:00:00
abstract::Cucumber (Cucumis sativus L., cv Poinsette) plants were sprayed with 20 millimolar 5-aminolevulinic acid and then incubated in the dark for 14 hours. The intact chloroplasts were isolated from the above plants in the dark and were exposed to weak light (250 micromoles per square meter per second). Within 30 minutes, p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.7
更新日期:1992-01-01 00:00:00
abstract::Maximum freezing tolerance of Arabidopsis thaliana L. Heyn (Columbia) was attained after 1 week of cold acclimation at 2[deg]C. During this time, there were significant changes in both the lipid composition of the plasma membrane and the freeze-induced lesions that were associated with injury. The proportion of phosph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.1.15
更新日期:1995-09-01 00:00:00
abstract::A nucleoside triphosphatase (NTPase) present in highly purified preparations of pea nuclei was partially characterized. The activity of this enzyme was stimulated by divalent cations (Mg2+ = Mn2+ > Ca2+), but was not affected by the monovalent cations, Na+ and K+. The Mg(2+)-dependent activity was further stimulated...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.609
更新日期:1986-01-01 00:00:00
abstract::Radioactive glutamic acid and N-acetylglutamic acid have been incubated with normal and wilted leaf discs and radioactivity recovered in uncombined proline. The discs which had been placed under moisture stress incorporated considerably more label in uncombined proline than did normal discs.In extracts of swiss chard ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.7.1023
更新日期:1969-07-01 00:00:00
abstract::Glycoprotein nonspecific elicitor (NSE) and a specific elicitor preparation from intercellular fluids (SE) of tomato (Lycopersicon esculentum Mill. cv Bonny Best or Potentate) infected with race 2.4.5 of Cladosporium fulvum Cooke [syn. Fulvia fulva (Cooke) Ciferri] were injected into cv Sonatine (resistant to race 2.4...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.867
更新日期:1989-07-01 00:00:00
abstract::In this study, we generated transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochrome b6f (cyt b6f) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cyt f (PetA) and cyt b6 (PetB), core proteins of t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00622
更新日期:2017-09-01 00:00:00
abstract::The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- and sterol-rich membrane domains. The aim of this work was to obtain evidence for lipid domain phenomena in plant membranes. A protocol to prepare Triton X-100 detergent-resistant me...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053041
更新日期:2005-01-01 00:00:00
abstract::A new indole-3-acetic acid metabolite was isolated from broad bean (Vicia faba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-(O-beta-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnet...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.3.667
更新日期:1985-11-01 00:00:00
abstract::Cell-cell and extracellular cell matrix (ECM) interactions provide cells with information essential for controlling morphogenesis, cell-fate specification, and cell death. In animals, one of the major groups of enzymes that degrade the ECM is the matrix metalloproteinases (MMPs). Here, we report the characterization o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.917
更新日期:2000-07-01 00:00:00
abstract::A study of the distribution of lectins over different vegetative tissues of barley (Hordeum vulgare L.) plants, which were grown under normal crop conditions, indicated that lectin occurs in roots, leaves, and developing ears. Isolation and characterization of both root and leaf lectins led to the conclusions (a) that...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.384
更新日期:1985-06-01 00:00:00
abstract::Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.2.597
更新日期:1995-06-01 00:00:00
abstract::Oleoresin (pitch) is a defensive secretion composed of monoterpene olefins (turpentine) and diterpene resin acids (rosin) that is produced in grand fir (Abies grandis Lindl.) stems in response to wounding. Monoterpene and diterpene biosynthesis are coordinately induced in wounded stems as determined by monitoring the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.999
更新日期:1994-11-01 00:00:00
abstract::White light (25 watts per square meter) induced an increase in plasma membrane K(+)-channel activity and a 30- to 70-millivolt transient membrane depolarization (completed in 2-3 minutes) in Arabidopsis thaliana leaf mesophyll cells. Transport characteristics of three types of ion channels in the plasma membrane were ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.1.96
更新日期:1992-05-01 00:00:00
abstract::Chlorophyll a fluorescence of Synechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO(2) fixation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of Q(a), the primary acceptor of photosyst...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.6
更新日期:1988-09-01 00:00:00
abstract::We recently identified a cDNA encoding a putative isovaleryl-coenzyme A (CoA) dehydrogenase in Arabidopsis (AtIVD). In animals, this homotetrameric enzyme is located in mitochondria and catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. Ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.2.601
更新日期:2001-06-01 00:00:00
abstract::Storage protein hydrolysis in the cotyledons of germinating mung beans (Phaseolus aureus Roxb.) was examined by histochemical techniques, and the autolytic capacity of isolated protein bodies was studied with biochemical methods. The localization of endopeptidase activity within the cotyledons was studied using an Ind...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.2.292
更新日期:1975-08-01 00:00:00
abstract::Stimulation of stem elongation on green cuttings of Phaseolus aureus by malformin occurred only in red light and was specifically reversible by subsequent treatment with far red radiation. Inhibition of stem elongation of etiolated cuttings by malformin in the dark was alleviated by red light and was repeatedly revers...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.4.622
更新日期:1977-10-01 00:00:00
abstract::Amino compounds representative of the major N solutes of xylem sap were pulse-fed (10 to 20 minutes) singly in (14)C-labeled form to cut transpiring shoots of white lupin (Lupinus albus L.). (14)C distribution was studied by autoradiography and radioassays of phloem sap, leaflet tissues, and shoot parts harvested at i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1076
更新日期:1979-06-01 00:00:00
abstract::Several angiosperm plant genomes, including Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), poplar (Populus trichocarpa), and grapevine (Vitis vinifera), have been sequenced, but the lack of reference genomes in gymnosperm phyla reduces our understanding of plant evolution and restricts the potential impacts ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.179663
更新日期:2011-09-01 00:00:00
abstract::Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082743
更新日期:2006-07-01 00:00:00
abstract::Chlorophyll levels in l-cm sections of the youngest fully expanded leaves of normal (Y) Festuca pratensis L. declined almost to zero over a period of 6 days after excision. Chlorophyll in a mutant genotype (NY) remained near the initial level for the whole of this period. Abscisic acid promoted pigment loss in Y but h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.3.438
更新日期:1975-09-01 00:00:00
abstract::A method has been devised for loading chloroplast inner envelope vesicles prepared from pea (Pisum sativum L. var Progress No. 9) or spinach (Spinacia oleracea L.) with 8-hydroxypyrene-1,3,6-trisulfonate (pyranine), a membrane impermeant, fluorescent pH indicator. Two known proton-linked transport activities of the in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.999
更新日期:1988-04-01 00:00:00
abstract::The cyanobacterial CO2-concentrating mechanism (CCM) allows photosynthesis to proceed in CO2-limited aquatic environments, and its activity is modulated in response to inorganic carbon (Ci) availability. Real-time reverse transcriptase-PCR analysis was used to examine the transcriptional regulation of more than 30 CCM...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.029728
更新日期:2003-12-01 00:00:00
abstract::Mitogen-activated protein kinases (MAPKs) are fundamental components of the plant innate immune system. MPK3 and MPK6 are Arabidopsis (Arabidopsis thaliana) MAPKs activated by pathogens and elicitors such as oligogalacturonides (OGs), which function as damage-associated molecular patterns, and flg22, a well-known micr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.174003
更新日期:2011-10-01 00:00:00
abstract::Pretreatment with pertussis toxin or microinjection of guanosine- 5[prime]-(3-thiotriphosphate) (GTP-[gamma]-S) into guard cells in peeled epidermis of Commelina communis L. promoted stomatal opening under subsaturating white light. Guanosine-5[prime]-(2-thiodiphosphate) (GDP-[beta]-S) and adenosine-5[prime]-(3-thiotr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.1.95
更新日期:1993-05-01 00:00:00
abstract::Cytochrome P450s (P450s) constitute one of the major classes of enzymes that are responsible for detoxification of exogenous molecules both in animals and plants. On the basis of its inducibility by exogenous chemicals, we recently isolated a new plant P450, CYP76B1, from Jerusalem artichoke (Helianthus tuberosus) and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.3.1049
更新日期:1998-11-01 00:00:00
abstract::Experiments were conducted on Nicotiana tabacum, L. to study the relation in the grana among chlorophylls, carotenoids, and proteins. The effect of iron chlorosis on protein and pigment synthesis was studied at different stages of chlorosis using glycine-U-C(14). Pigments were separated by thin layer chromatography.Ch...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.3.415
更新日期:1966-03-01 00:00:00
abstract::A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp). Replacement of the serine at position 65 with a threonine (S65Tpgfp) or a cy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.893
更新日期:1996-11-01 00:00:00
abstract::Cool season crops face intermittent drought. Exposure to drought and other abiotic stresses is known to increase tolerance of the plants against subsequent exposure to such stresses. Storage of environmental signals is also proposed. Preexposure to a dehydration shock improved adaptive response during subsequent dehyd...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.043141
更新日期:2004-07-01 00:00:00