Abstract：
:The Ras gene family has been implicated in the development of many human epithelial cancers. Mutations in K-ras or its downstream mediator BRAF have been detected in about two thirds of low-grade serous carcinomas and borderline serous tumors; mutations in K-ras are also often present in benign and invasive mucinous ovarian cancers. Although the oncogenic allele H-ras(V12) is present in only approximately 6% of ovarian cancers, physiologically activated H-ras protein is commonly detected in human ovarian cancer, presumably because of an increase in upstream signals from tyrosine kinase growth factor receptors such as Her-2/neu, despite the lack of a Ras mutation. The mechanisms by which ras oncogenes transform human epithelial cells are not clear. The methods described here are what we use to culture human ovarian surface epithelial cells, to immortalize those cells, and to transform the immortalized cells with oncogenic H-ras or K-ras. These Ras-transformed human ovarian surface epithelial cells form tumors in nude mice and recapitulate many features of human ovarian cancer, thus providing an excellent model system for studying the initiation and progression of human ovarian cancer.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Rosen DG,Yang G,Bast RC Jr,Liu Jdoi
10.1016/S0076-6879(05)07052-7subject
Has Abstractpub_date
2006-01-01 00:00:00pages
660-76eissn
0076-6879issn
1557-7988pii
S0076-6879(05)07052-7journal_volume
407pub_type
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journal_title：Methods in enzymology
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