New construct approaches for efficient gene silencing in plants.

abstract：:N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...

journal_title：Plant physiology

authors： Chen CM,Leisner SM

更新日期：1984-06-01 00:00:00

abstract：:The structure of phosphatidylinositol in barley (Hordeum vulgare) aleurone layers was investigated by chemical degradation. In vivo myo-[2-(3)H]inositol-labeled phosphatidylinositol was first converted to glycerophosphoinositol and, subsequently, after removal of the glycerol moiety, to inositol monophosphate. Here, w...

journal_title：Plant physiology

authors： Murthy PP,Pliska-Matyshak G,Keranen LM,Lam P,Mueller HH,Bhuvarahamurthy N

更新日期：1992-04-01 00:00:00

abstract：:The distribution of solutes in the various cells of sugar beet (Beta vulgaris L.) source leaves, petioles, and sink leaves was studied in tissue prepared by freeze-substitution. The differences in degree of cryoprotection indicated that sieve elements and companion cells of the source leaf, petiole, and sink leaf cont...

journal_title：Plant physiology

authors： Geiger DR,Giaquinta RT,Sovonick SA,Fellows RJ

更新日期：1973-12-01 00:00:00

abstract：:The sites of action of several compounds, reported to inhibit CO(2) fixation by chloroplast preparations were located by developing assays in lettuce chloroplasts to test their effect on partial reactions of the carbon cycle and on carbonic anhydrase. The results indicated that: d, l-glyceral-dehyde and 5'-AMP inhibit...

journal_title：Plant physiology

authors： Bamberger ES,Avron M

更新日期：1975-10-01 00:00:00

abstract：:Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...

journal_title：Plant physiology

authors： Fowler JL,Morgan PW

更新日期：1972-04-01 00:00:00

abstract：:The presence of chloroplasts in guard cells from leaf epidermis, coleoptile, flowers, and albino portions of variegated leaves was established by incident fluorescence microscopy, thus confirming the notion that guard cell chloroplasts are remarkably conserved. Room temperature emission spectra from a few chloroplasts...

journal_title：Plant physiology

authors： Zeiger E,Armond P,Melis A

更新日期：1981-01-01 00:00:00

abstract：:Transcription of the Arabidopsis thaliana gene encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (RCA) is organ-specific, light-responsive, and regulated by the circadian clock. RCA is transcribed throughout the green parts of the plant, but not in roots and petals. Responses elicited by shor...

journal_title：Plant physiology

authors： Liu Z,Taub CC,McClung CR

更新日期：1996-09-01 00:00:00

abstract：:Ultraviolet-B (UV-B) photons can cause substantial cellular damage in biomolecules, as is well established for DNA. Because RNA has the same absorption spectrum for UV as DNA, we have investigated damage to this cellular constituent. In maize (Zea mays) leaves, UV-B radiation damages ribosomes by crosslinking cytosoli...

journal_title：Plant physiology

authors： Casati P,Walbot V

更新日期：2004-10-01 00:00:00

abstract：:A method to fractionate corn (Zea mays L. B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondrial enzyme activities. The Krebs cycle ...

journal_title：Plant physiology

authors： Hayes MK,Luethy MH,Elthon TE

更新日期：1991-12-01 00:00:00

abstract：:Using photoacoustic spectroscopy, state 1-state 2 transitions were demonstrated in vivo in intact sugar maple leaves (Acer saccharum Marsh.) by following the changes in energy storage of photosystems (PS) I and II. Energy storage measured with 650 nm modulated light (light 2) in the presence of background white light ...

journal_title：Plant physiology

authors： Veeranjaneyulu K,Charland M,Charlebois D,Leblanc RM

更新日期：1991-09-01 00:00:00

abstract：:Low red light/far-red light ratio (R:FR) serves as an indicator of impending competition and has been demonstrated to suppress branch development. The regulation of Arabidopsis (Arabidopsis thaliana) rosette bud outgrowth by the R:FR and the associated mechanisms were investigated at several levels. Growth under low R...

journal_title：Plant physiology

authors： Reddy SK,Holalu SV,Casal JJ,Finlayson SA

更新日期：2013-10-01 00:00:00

abstract：:Membrane preparation (sedimenting between 13,000(g) and 80,000(g)) of germinating radish seeds (Raphanus sativus L.) was active in hydrolyzing ATP and, to a lesser extent, a variety of other phosphorylated compounds. Dicyclohexylcarbodiimide (DCCD) and diethylstilbestrol significantly inhibited the ATPase activity (40...

journal_title：Plant physiology

authors： Cocucci M,Ballarin-Denti A

更新日期：1981-08-01 00:00:00

abstract：:Plant cystatins are inhibitors of cysteine-proteases of the papain C1A and legumain C13 families. Cystatin data from multiple plant species have suggested that these inhibitors act as defense proteins against pests and pathogens and as regulators of protein turnover. In this study, we characterize the entire cystatin ...

journal_title：Plant physiology

authors： Martinez M,Cambra I,Carrillo L,Diaz-Mendoza M,Diaz I

更新日期：2009-11-01 00:00:00

abstract：:Severe Arabidopsis (Arabidopsis thaliana) gibberellin (GA)-deficient mutant ga1-3 fails to germinate and is impaired in floral organ development. In contrast, the ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 mutant confers GA-independent seed germination and floral development. This fact suggests that GA-regulated transcriptomes...

journal_title：Plant physiology

authors： Cao D,Cheng H,Wu W,Soo HM,Peng J

更新日期：2006-10-01 00:00:00

abstract：:THE PRESENT STUDY COMPARES THE BINDING AND INHIBITORY ACTIVITY OF TWO PHOTOSYSTEM II INHIBITORS: 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron [DCMU]) and 2-chloro-4-(ethylamine)-6-(isopropyl amine)-S-triazene (atrazine). Chloroplasts isolated from naturally occurring triazine-susceptible and triazine-resistant biot...

journal_title：Plant physiology

authors： Pfister K,Radosevich SR,Arntzen CJ

更新日期：1979-12-01 00:00:00

abstract：:Continuous wave nuclear magnetic resonance (NMR) studies indicated that the line width of the water absorption peak (Deltav(1/2)) from crowns of winter and spring wheat (Triticum aestivum L.) increased during cold acclimation. There was a negative correlation between Deltav(1/2) and crown water content, and both of th...

journal_title：Plant physiology

authors： Gusta LV,Fowler DB,Chen P

更新日期：1979-04-01 00:00:00

abstract：:Soybeans (Glycine max) exposed to chronic levels of ozone showed a linear decrease in biomass with increasing concentration. The foliar free sterols increased while the steryl ester, and the steryl glycosides, a minor component, decreased with increasing pollutant concentration. Of the free sterols, stigmasterol showe...

journal_title：Plant physiology

authors： Grunwald C,Endress AG

更新日期：1985-01-01 00:00:00

abstract：:Fatty acid desaturases regulate the unsaturation status of cellular lipids. They comprise two distinct evolutionary lineages, a soluble class found in the plastids of higher plants and an integral membrane class found in plants, yeast (Saccharomyces cerevisiae), animals, and bacteria. Both classes exhibit a dimeric qu...

journal_title：Plant physiology

authors： Liu Q,Chai J,Moche M,Guy J,Lindqvist Y,Shanklin J

更新日期：2015-09-01 00:00:00

abstract：:The actin cytoskeleton is hypothesized to play a major role in gravity perception and transduction mechanisms in roots of plants. To determine whether actin microfilaments (MFs) are involved in these processes in stem-like organs, we studied gravitropism in Arabidopsis inflorescence stems and hypocotyls. Localization ...

journal_title：Plant physiology

authors： Yamamoto K,Kiss JZ

更新日期：2002-02-01 00:00:00

abstract：:Heat treatment (37 degrees C) of transgenic tobacco (Nicotiana tabacum) plants led to a reversible reduction or complete loss of transgene-encoded activities in about 40% of 10 independent transformants carrying the luciferase-coding region fused to the 355 cauliflower mosaic virus or the soybean small subunit promote...

journal_title：Plant physiology

authors： Neumann K,Dröge-Laser W,Köhne S,Broer I

更新日期：1997-11-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance in model plant species such as Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum). However, the importance of MAPK signaling pathways in the disease resistance of crops is still largely uninvestigated. To better...

journal_title：Plant physiology

authors： Liu JZ,Horstman HD,Braun E,Graham MA,Zhang C,Navarre D,Qiu WL,Lee Y,Nettleton D,Hill JH,Whitham SA

更新日期：2011-11-01 00:00:00

abstract：:The translocation of radioactive photosynthate was studied in blades detached from plants which had been grown in nutrient solution with and without potassium. Basipetal translocation decreased in the blades of plants deprived of potassium, even when deficiency symptoms were not visible. In such slightly potassium-def...

journal_title：Plant physiology

authors： Hartt CE

更新日期：1970-02-01 00:00:00

abstract：:Experiments were conducted to determine whether sucrose synthase (SuSy) was phosphorylated in the elongation zone of maize (Zea mays L.) leaves. The approximately 90-kD subunit of SuSy was 32P-labeled on seryl residue(s) when excised shoots were fed [32P]orthophosphate. Both isoforms of SuSy (the SS1 and SS2 proteins)...

journal_title：Plant physiology

authors： Huber SC,Huber JL,Liao PC,Gage DA,McMichael RW Jr,Chourey PS,Hannah LC,Koch K

更新日期：1996-10-01 00:00:00

abstract：:Carbonic anhydrase (CA) (EC 4.2.1.1) enzymes catalyze the reversible hydration of CO2, a reaction that is important in many physiological processes. We have cloned and sequenced a full-length cDNA encoding an intracellular beta-CA from the unicellular green alga Coccomyxa. Nucleotide sequence data show that the isolat...

journal_title：Plant physiology

authors： Hiltonen T,Björkbacka H,Forsman C,Clarke AK,Samuelsson G

更新日期：1998-08-01 00:00:00

abstract：:It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transien...

journal_title：Plant physiology

authors： Moshkov IE,Novikova GV,Mur LA,Smith AR,Hall MA

更新日期：2003-04-01 00:00:00

abstract：:p-Hydroxybenzoic acid (pHBA) is the major monomer in liquid crystal polymers. In this study, the Escherichia coli ubiC gene that codes for chorismate pyruvate-lyase (CPL) was integrated into the tobacco (Nicotiana tabacum) chloroplast genome under the control of the light-regulated psbA 5' untranslated region. CPL cat...

journal_title：Plant physiology

authors： Viitanen PV,Devine AL,Khan MS,Deuel DL,Van Dyk DE,Daniell H

更新日期：2004-12-01 00:00:00

abstract：:Mutant strain 1073 of Lemna perpusilla is concluded to be blocked between plastoquinone and cytochrome f in the photosynthetic electron transport system. The location of the block is based on the following observations of activities in chloroplasts isolated from the mutant and wild-type plants. (a) Relative to wild ty...

journal_title：Plant physiology

authors： Shahak Y,Posner HB,Avron M

更新日期：1976-04-01 00:00:00

abstract：:The oat (Avena sativa L.) seed globulin was found to be synthesized in vitro as 60,000 to 64,000 dalton precursors. In vivo protein labeling yielded polypeptides of 58,000 to 62,000 daltons, suggesting cleavage of signal sequences from the precursors. Further cleavage is apparently required to separate the alpha and b...

journal_title：Plant physiology

authors： Brinegar AC,Peterson DM

更新日期：1982-12-01 00:00:00

abstract：:Freshly isolated cotyledons from 10-day developing pea (Pisum sativum) seeds were fed radiolabeled precursors for 5 hours, and the specific radioactivity of the free and total protein amino acids was determined using a dansylation procedure. When the seven most abundant amino acids in phloem exudate of pea fruits (asp...

journal_title：Plant physiology

authors： Macnicol PK

更新日期：1977-09-01 00:00:00

abstract：:Enhanced disease susceptibility1 (EDS1) and phytoalexin deficient4 (PAD4) are well-known regulators of both basal and resistance (R) protein-mediated plant defense. We identified two EDS1-like (GmEDS1a/GmEDS1b) proteins and one PAD4-like (GmPAD4) protein that are required for resistance signaling in soybean (Glycine m...

journal_title：Plant physiology

authors： Wang J,Shine MB,Gao QM,Navarre D,Jiang W,Liu C,Chen Q,Hu G,Kachroo A

更新日期：2014-07-01 00:00:00