Abstract:
:The effect of granulocyte-macrophage colony stimulatory factors (GM-CSF), acidic isoferritins, and E-type prostaglandins on the detection of the cycle status of human granulocyte-macrophage progenitor cells (CFU-GM) was investigated. Bone marrow cells were pulse-treated with control medium or high specific activity tritiated thymidine [3HTdr) and subsequently plated over feeder layers containing mononuclear blood leukocytes prepared in the absence or presence of anti-acidic isoferritins and/or indomethacin, or plated in the presence of medium conditioned by placental cell or GCT-conditioned media free of acidic isoferritins and prostaglandin-E. The presence of anti-acidic isoferritins and/or indomethacin in the blood leukocyte feeder layers increased the detectable stimulatory capacity of these cells and permitted detection of a larger proportion of marrow CFU-GM in cycle than in control cultures. The cycle status was not influenced by GM-CSF in conditioned medium regardless of the dilution of conditioned medium used to stimulate colony formation. This suggests that GM-CSF, supplied to the cells after treatment with 3HTdr, does not itself influence the detection of CFU-GM in cycle, but using sources of GM-CSF that contain acidic isoferritins or prostaglandin-E will underestimate the actual number of CFU-GM in S-phase.
journal_name
Bloodjournal_title
Bloodauthors
Broxmeyer HEsubject
Has Abstractpub_date
1982-10-01 00:00:00pages
1042-5issue
4eissn
0006-4971issn
1528-0020journal_volume
60pub_type
杂志文章相关文献
BLOOD文献大全abstract::We investigated 17 patients (12 males and 5 females, ages 2 to 57 years old) with posttransfusion non-A, non-B hepatitis to determine relationships between clinical courses and hepatitis C virus (HCV) markers. The patients were grouped according to time course of abnormal serum alanine aminotransferase (ALT) levels in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-03-15 00:00:00
abstract::Interleukin 21 (IL-21) is a member of the common gamma-chain family of cytokines, which influence a broad spectrum of immunologic responses. A number of studies have examined the function of IL-21, but its specific role in Th1/Th2-cell differentiation and related effector responses remains to be clarified. Thus, we ge...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-05-021600
更新日期:2007-03-01 00:00:00
abstract::Although the genes for four hematopoietic colony-stimulating factors (CSFs) have been cloned, neither the mechanism of the regulation of their production nor their cellular origins have been established with certainty. Monocytes are known to produce colony-stimulating and burst-promoting activities, as well as several...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::The inv(16)(p13q22) and t(16;16)(p13;q22) cytogenetic abnormalities occur commonly in acute myeloid leukemia (AML), typically associated with French-American-British (FAB) AML-M4Eo subtype. Reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been recently developed to detect the presence of severa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-03-15 00:00:00
abstract::Plasma fibronectin enhances platelet thrombus formation on surfaces coated with collagen. We investigated the role of fibronectin assembly in this process. Platelets adherent to fibrillar type I collagen, but not platelets adherent to von Willebrand factor (VWF), supported assembly of plasma fibronectin under static c...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-02-002063
更新日期:2006-10-01 00:00:00
abstract::Most bone marrow (BM) malignancies develop in association with an angiogenic phenotype and increased numbers of endothelial cells. The molecular mechanisms involved in the modulation and recruitment of BM endothelium are largely unknown and may provide novel therapeutic targets for neoplastic diseases. We observed tha...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-06-2208
更新日期:2006-01-01 00:00:00
abstract::Characterization of hematopoietic cells and measurement of their proliferative potential is critical in many research and clinical applications. Because in vivo assay of human cells is not possible and xenogeneic assays are not yet routine, in vitro assays such as the long-term culture-initiating cell (LTC-IC) assay h...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Epigenetic events that are essential drivers of lymphocyte transformation remain incompletely characterized. We used models of Epstein-Barr virus (EBV)-induced B-cell transformation to document the relevance of protein arginine methyltransferase 5 (PRMT5) to regulation of epigenetic-repressive marks during lymphomagen...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-12-619783
更新日期:2015-04-16 00:00:00
abstract::IL-12 exerts several regulatory effects on natural killer (NK) cells by activating IL-12 signaling. IL-12 signaling is tightly auto-regulated to control its onset and termination, with prolonged IL-12 treatment resulting in IL-12 hyporesponsiveness. However, the mechanisms underlying IL-12 auto-regulation are still un...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-05-356162
更新日期:2011-12-22 00:00:00
abstract::Antibody-induced antigenic modulation (AIAM) of CD10 and CD19 was studied on NALM-6, RAJI, and JOK-1 cell lines using fluorescence microscopy (FM), flow cytometry (FCM), and immunoelectron microscopy (IEM). Cross-linking with monoclonal antibodies (MoAbs) induced rapid redistribution of CD10 and CD19 on the cell surfa...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-03-15 00:00:00
abstract::The report of the so-called Berlin patient cured of HIV with hematopoietic stem cell transplantation and a few other studies raised tremendous hope, excitement, and curiosity in the field. The National Heart, Lung and Blood Institute of the National Institutes of Health convened a Working Group to address emerging hea...
journal_title:Blood
pub_type:
doi:10.1182/blood-2013-07-518316
更新日期:2013-10-31 00:00:00
abstract::Patients who are chronically infected with hepatitis C virus (HCV) often develop mixed cryoglobulinemia (MC), a B-cell proliferative disorder with polyclonal activation and autoantibody production. We investigated if MC is associated with a deficit of CD4(+)CD25(+) immunoregulatory T (Treg) cells, which have been show...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-07-2598
更新日期:2004-05-01 00:00:00
abstract::Primary mediastinal large B-cell lymphoma (PMBCL) is a recognized non-Hodgkin lymphoma entity with unique pathologic, clinical, and molecular characteristics distinct from those of other diffuse large B-cell lymphomas. Immunohistochemical characterization and molecular studies strongly suggest that PMBCL is of germina...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2011-05-326538
更新日期:2011-09-08 00:00:00
abstract::Erythroid cell iron and transferrin uptake and release was studied in the anemia of the Belgrade laboratory rat (gene symbol, b), an autosomal recessive trait characterized by hypochromia and hyperferrinemia. When reticulocyte-rich red cells were incubated in vitro with doubly (59Fe, 125I) labeled transferrin, b/b cel...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Myelodysplastic and leukemic stem cell clones that evolve in children and adults with Fanconi anemia universally bear complex cytogenetic abnormalities. The abnormalities are generally recurring deletions or chromosomal loss and involve precisely the same chromosomes with the same frequency as has been described in ma...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-09-2781
更新日期:2003-07-01 00:00:00
abstract::NRAS is frequently mutated in hematologic malignancies. We generated Mx1-Cre, Lox-STOP-Lox (LSL)-Nras(G12D) mice to comprehensively analyze the phenotypic, cellular, and biochemical consequences of endogenous oncogenic Nras expression in hematopoietic cells. Here we show that Mx1-Cre, LSL-Nras(G12D) mice develop an in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-04-280750
更新日期:2011-02-10 00:00:00
abstract::2-Methoxyestradiol (2-ME), a new anticancer agent currently in clinical trials, has been demonstrated to inhibit superoxide dismutase (SOD) and to induce apoptosis in leukemia cells through a free radical-mediated mechanism. Because the accumulation of superoxide (O(2)-) by inhibition of SOD depends on the cellular ge...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-08-2512
更新日期:2003-05-15 00:00:00
abstract::Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels has been used in routine practice. We used limiting dilution analysis, which measures clonable T cells, to assess the influence of 500 to 3,000 cGy...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-03-15 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) play an important role in the regulation of the immune response. MDSC expansion occurs in many circumstances, including cancer, inflammation, stresses, and transplant tolerance. Liver transplants in mice are spontaneously accepted, but hepatocyte transplants are acutely rejecte...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-06-440214
更新日期:2013-03-07 00:00:00
abstract::It has previously been shown that human granulocyte-macrophage colony-stimulating factor (GM-CSF) can be fused to a truncated diphtheria toxin (DT) to produce a recombinant fusion toxin that kills GM-CSF receptor-bearing cells. We now report that DT388-GM-CSF induces apoptosis and inhibition of colony formation in sem...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::The mechanical properties of sickle erythrocyte membranes were evaluated in the ektacytometer. When ghosts from the total red blood cell population were examined, the rigidity of the resealed ghosts and their rate of fragmentation by shear stress (t1/2) were normal. However, fractionation on Stractan density gradients...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-04-15 00:00:00
abstract::Sickle cell anemia (SCA) is an inherited disorder of beta-globin, resulting in red blood cell rigidity, anemia, painful crises, organ infarctions, and reduced life expectancy. Allogeneic blood or marrow transplantation (BMT) can cure SCA but is associated with an 8% to 10% mortality rate, primarily from complications ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.12.3960
更新日期:2001-06-15 00:00:00
abstract::Mice lacking all 3 Vav proteins fail to produce significant numbers of recirculating follicular or marginal zone B cells. Those B cells that do mature have shortened lifespans. The constitutive nuclear factor-kappaB (NF-kappaB) activity of resting naive B cells required Vav function and expression of cellular reticulo...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-12-4894
更新日期:2005-10-01 00:00:00
abstract::MEIS1 is a three-amino acid loop extension class homeodomain-containing homeobox (HOX) cofactor that plays key roles in normal hematopoiesis and leukemogenesis. Expression of Meis1 is rate-limiting in MLL-associated leukemias and potently interacts with Hox and NUP98-HOX genes in leukemic transformation to promote sel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-06-225573
更新日期:2010-05-20 00:00:00
abstract::Adenovirus infections occur in 5% to 21% of patients following stem cell transplantation (SCT), with an associated mortality of up to 50%. However, a lack of prospective studies has hampered further developments in the understanding and management of this infection in the posttransplantation setting. We prospectively ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-02-0377
更新日期:2002-09-01 00:00:00
abstract::Chimeric antigen receptor (CAR) T cells have radically improved the treatment of B cell-derived malignancies by targeting CD19. The success has not yet expanded to treat acute myeloid leukemia (AML). We developed a Sequentially Tumor-Selected Antibody and Antigen Retrieval (STAR) system to rapidly isolate multiple nan...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019002779
更新日期:2020-03-05 00:00:00
abstract::Immunosenescence is the functional deterioration of the immune system during natural aging. Despite increased susceptibility to bacterial infections in older adults, age-associated changes to neutrophil responses are only partially understood, and neutrophil migration has not been characterized in detail. Here we desc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-08-519520
更新日期:2014-01-09 00:00:00
abstract::We evaluated the potential role of photoradiation therapy with a benzoporphyrin derivative, monoacid ring A (BPD-MA), and dihematoporphyrin ether (DHE), for the ex vivo purging of residual tumor cells from autologous bone marrow (BM) grafts. BPD-MA and DHE photosensitizing activity was tested against two human large-c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::We tested the ability of CD34+lin- precursor cells isolated from marrow after treatment with 4-hydroperoxycyclophosphamide (4HC) to generate colony-forming cells (CFC). In liquid cultures, recombinant human stem cell factor (SCF), in combination with interleukin-1 (IL-1), IL-3, IL-6, granulocyte-macrophage colony-stim...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Two sisters in whom a diagnosis of Fanconi's anemia was made at ages 12 and 18 subsequently developed acute nonlymphocytic leukemia (ANLL). A third sibling had previously died at age 11 of apparent sepsis. Both sisters had cytogenetic studies that showed increased chromosomal breakage and a 46,XX karyotype, but subseq...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00