Impact of fibronectin assembly on platelet thrombus formation in response to type I collagen and von Willebrand factor.


:Plasma fibronectin enhances platelet thrombus formation on surfaces coated with collagen. We investigated the role of fibronectin assembly in this process. Platelets adherent to fibrillar type I collagen, but not platelets adherent to von Willebrand factor (VWF), supported assembly of plasma fibronectin under static conditions. At a shear rate of 1250 s(-1), platelets adherent to collagen assembled coperfused plasma fibronectin and formed larger thrombi in a fibronectin-concentration-dependent manner, with a maximum effect at 250 mug/mL. Enhanced thrombus formation on collagen was blocked by a peptide that binds to the N-terminal region of fibronectin and inhibits fibronectin assembly. Cross-linking of fibronectin to collagen prior to exposure to platelets had no effect on thrombus formation. Collagen-induced platelet thrombus formation at a shear rate of 5000 s(-1) required coperfusion with VWF and did not result in assembly of coperfused fibronectin. VWF-mediated increase in platelet thrombi on collagen was not enhanced and indeed was somewhat attenuated by coperfused fibronectin at a shear rate of 5000 s(-1). These results indicate that, at moderately high but not very high shear rates, fibronectin assembly in platelet aggregates that form in response to collagen enhances thrombus formation and serves as an alternative to VWF-mediated enhancement.






Cho J,Mosher DF




Has Abstract


2006-10-01 00:00:00
















  • Platelets are necessary for airway wall remodeling in a murine model of chronic allergic inflammation.

    abstract::Asthma is associated with airway remodeling. Evidence of platelet recruitment to the lungs of asthmatics after allergen exposure suggests platelets participate in various aspects of asthma; although their importance is unknown in the context of airway remodeling, their involvement in atherosclerosis is established. St...


    pub_type: 杂志文章


    authors: Pitchford SC,Riffo-Vasquez Y,Sousa A,Momi S,Gresele P,Spina D,Page CP

    更新日期:2004-01-15 00:00:00

  • Caspase activation is required for nitric oxide-mediated, CD95(APO-1/Fas)-dependent and independent apoptosis in human neoplastic lymphoid cells.

    abstract::Nitric oxide (NO), an important effector molecule involved in immune regulation and host defense, was shown to induce apoptosis in lymphoma cells. In the present report the NO donor glycerol trinitrate was found to induce apoptosis in Jurkat cells that are sensitive to CD95-mediated kill. In contrast, a CD95-resistant...


    pub_type: 杂志文章


    authors: Chlichlia K,Peter ME,Rocha M,Scaffidi C,Bucur M,Krammer PH,Schirrmacher V,Umansky V

    更新日期:1998-06-01 00:00:00

  • EBV-tissue positive primary CNS lymphoma occurring after immunosuppression is a distinct immunobiological entity.

    abstract::Primary central nervous system lymphoma (PCNSL) is confined to the brain, eyes, and cerebrospinal fluid without evidence of systemic spread. Rarely, PCNSL occurs in the context of immunosuppression, e.g. post-transplant lymphoproliferative disorders (PTLD) or HIV (AIDS-related PCNSL). These cases are poorly characteri...


    pub_type: 杂志文章


    authors: Gandhi MK,Hoang T,Law SC,Brosda S,O'Rourke K,Tobin JWD,Vari F,Murigneux V,Fink JL,Gunawardana J,Gould CM,Oey H,Bednarska K,Delecluse S,Trappe RU,de Long LM,Sabdia MB,Bhagat G,Hapgood G,Blyth E,Clancy LE,Wight J

    更新日期:2020-11-17 00:00:00

  • Rituximab for the treatment of refractory autoimmune hemolytic anemia in children.

    abstract::Autoimmune hemolytic anemia (AIHA) in children is sometimes characterized by a severe course, requiring prolonged administration of immunosuppressive therapy. Rituximab is able to cause selective in vivo destruction of B lymphocytes, with abrogation of antibody production. In a prospective study, we have evaluated the...


    pub_type: 临床试验,杂志文章


    authors: Zecca M,Nobili B,Ramenghi U,Perrotta S,Amendola G,Rosito P,Jankovic M,Pierani P,De Stefano P,Bonora MR,Locatelli F

    更新日期:2003-05-15 00:00:00

  • The development of lymphomas in families with autoimmune lymphoproliferative syndrome with germline Fas mutations and defective lymphocyte apoptosis.

    abstract::Lymphomas were studied in kindreds with autoimmune lymphoproliferative syndrome (ALPS; Canale-Smith syndrome), a disorder of lymphocyte homeostasis usually associated with germline Fas mutations. Fas (CD95/APO-1) is a cell surface receptor that initiates programmed cell death, or apoptosis, of activated lymphocytes. L...


    pub_type: 杂志文章


    authors: Straus SE,Jaffe ES,Puck JM,Dale JK,Elkon KB,Rösen-Wolff A,Peters AM,Sneller MC,Hallahan CW,Wang J,Fischer RE,Jackson CE,Lin AY,Bäumler C,Siegert E,Marx A,Vaishnaw AK,Grodzicky T,Fleisher TA,Lenardo MJ

    更新日期:2001-07-01 00:00:00

  • Solid-phase von Willebrand factor contains a conformationally active RGD motif that mediates endothelial cell adhesion through the alpha v beta 3 receptor.

    abstract::The interaction of von Willebrand factor (vWF) with the alpha v beta 3 integrin of human umbilical vein endothelial cells is dependent on the RGD sequence present at residues 1744-1746 of the mature vWF subunit. We compared vWF and its two dimeric fragments, SpIII (residues 1-1365) and SpII (residues 1366-2050), as ad...


    pub_type: 杂志文章


    authors: Denis C,Williams JA,Lu X,Meyer D,Baruch D

    更新日期:1993-12-15 00:00:00

  • Chemotherapy-only treatment effects on long-term neurocognitive functioning in childhood ALL survivors: a review and meta-analysis.

    abstract::Therapy for childhood acute lymphoblastic leukemia (ALL) is associated with 5-year survival rates of ∼90% even after largely eliminating cranial radiation. This meta-analysis assesses the long-term neurocognitive functioning after chemotherapy-only regimens among survivors of childhood ALL. We conducted a systematic r...


    pub_type: 杂志文章,meta分析,评审


    authors: Iyer NS,Balsamo LM,Bracken MB,Kadan-Lottick NS

    更新日期:2015-07-16 00:00:00

  • Comparison of activated protein C/protein S-mediated inactivation of human factor VIII and factor V.

    abstract::The proteolytic cleavage and subsequent inactivation of recombinant human factor VIII (rhFVIII) and human factor VIIIa (rhFVIIIa) by recombinant human activated protein C (rAPC) was analyzed in the presence and absence of human protein S and human factor V (FV). Membrane-bound rhFVIIIa spontaneously looses most of its...


    pub_type: 杂志文章


    authors: Lu D,Kalafatis M,Mann KG,Long GL

    更新日期:1996-06-01 00:00:00

  • RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy.

    abstract::Vaccination with antigen-presenting cells (APCs) engineered to mimic mechanisms of immune stimulation represents a promising approach for cancer immunotherapy. Dendritic cell vaccines have entered phase 3 testing in adult malignancies, but such vaccines in children have been limited. We demonstrate that CD40-activated...


    pub_type: 杂志文章


    authors: Coughlin CM,Vance BA,Grupp SA,Vonderheide RH

    更新日期:2004-03-15 00:00:00

  • Photochemical inactivation of cell-associated human immunodeficiency virus in platelet concentrates.

    abstract::Photochemical decontamination (PCD) of platelet concentrates, with adequate preservation of platelet function, has been shown using 8-methoxypsoralen (8-MOP) and long wavelength UV light (UVA). To further evaluate this technique, models for the inactivation of pathogenic human cell-associated viruses and integrated pr...


    pub_type: 杂志文章


    authors: Lin L,Londe H,Hanson CV,Wiesehahn G,Isaacs S,Cimino G,Corash L

    更新日期:1993-07-01 00:00:00

  • Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A.

    abstract::Neuropilin-1 (Npn-1) is a receptor shared by class 3 semaphorins and heparin-binding forms of vascular endothelial growth factor (VEGF), protein families that regulate endothelial and neuronal-cell function. Ligand interaction with Npn-1 dictates the choice of signal transducer; plexins transduce semaphorin signals, a...


    pub_type: 杂志文章


    authors: Narazaki M,Tosato G

    更新日期:2006-05-15 00:00:00

  • Residual vein thrombosis to establish duration of anticoagulation after a first episode of deep vein thrombosis: the Duration of Anticoagulation based on Compression UltraSonography (DACUS) study.

    abstract::Residual vein thrombosis (RVT) indicates a prothrombotic state and is useful for evaluating the optimal duration of oral anticoagulant treatment (OAT). Patients with a first episode of deep vein thrombosis, treated with OAT for 3 months, were managed according to RVT findings. Those with RVT were randomized to either ...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Siragusa S,Malato A,Anastasio R,Cigna V,Milio G,Amato C,Bellisi M,Attanzio MT,Cormaci O,Pellegrino M,Dolce A,Casuccio A,Bajardi G,Mariani G

    更新日期:2008-08-01 00:00:00

  • CD4+ T-cell alloreactivity toward mismatched HLA class II alleles early after double umbilical cord blood transplantation.

    abstract::Although double umbilical cord blood transplantation (dUCBT) in adult patients may be associated with less graft failure compared with single UCBT, hematopoietic recovery generally originates from a single cord blood unit (CBU). CBU predominance is still incompletely understood. We recently showed that blood CD4+ T-ce...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: Lamers CH,Wijers R,van Bergen CA,Somers JA,Braakman E,Gratama JW,Debets R,Falkenburg JH,Cornelissen JJ

    更新日期:2016-10-27 00:00:00

  • Regulatory control of the terminal complement proteins at the surface of human endothelial cells: neutralization of a C5b-9 inhibitor by antibody to CD59.

    abstract::Functionally inhibitory antibody to the plasma membrane complement inhibitor CD59 has been used to investigate control of the terminal complement proteins at the endothelial cell surface. Antibodies against purified human erythrocyte CD59 (polyclonal anti-CD59 and monoclonal antibodies [MoAbs] 1F1 and 1F5) were found ...


    pub_type: 杂志文章


    authors: Hamilton KK,Ji Z,Rollins S,Stewart BH,Sims PJ

    更新日期:1990-12-15 00:00:00

  • An important role of lymphatic vessel activation in limiting acute inflammation.

    abstract::In contrast to the established role of blood vessel remodeling in inflammation, the biologic function of the lymphatic vasculature in acute inflammation has remained less explored. We studied 2 established models of acute cutaneous inflammation, namely, oxazolone-induced delayed-type hypersensitivity reactions and ult...


    pub_type: 杂志文章


    authors: Huggenberger R,Siddiqui SS,Brander D,Ullmann S,Zimmermann K,Antsiferova M,Werner S,Alitalo K,Detmar M

    更新日期:2011-04-28 00:00:00

  • Differential requirements for the O-linked branching enzyme core 2 beta1-6-N-glucosaminyltransferase in biosynthesis of ligands for E-selectin and P-selectin.

    abstract::Selectins are carbohydrate-binding adhesion molecules that play important roles in control of leukocyte traffic. Glycosyltransferases involved in selectin ligand biosynthesis include the alpha1,3-fucosyltransferases FucT-VII and FucT-IV, one or more sialyltransferases, and at least one O-linked branching enzyme. Previ...


    pub_type: 杂志文章


    authors: Snapp KR,Heitzig CE,Ellies LG,Marth JD,Kansas GS

    更新日期:2001-06-15 00:00:00

  • A novel costimulatory signaling in human T lymphocytes by a splice variant of CD28.

    abstract::We have characterized a splice variant (isoform) of the human CD28 T cell costimulatory receptor. The nucleotide sequence of this CD28 isoform was identical to that of CD28 in the signal peptide, the transmembrane domain, and the cytoplasmic tail, but it was missing a large segment of the extracellular ligand-binding ...


    pub_type: 杂志文章


    authors: Hanawa H,Ma Y,Mikolajczak SA,Charles ML,Yoshida T,Yoshida R,Strathdee CA,Litchfield DW,Ochi A

    更新日期:2002-03-15 00:00:00

  • Induction of fibrinogen binding and platelet aggregation as a potential intrinsic property of various glycoprotein IIb/IIIa (alphaIIbbeta3) inhibitors.

    abstract::The blockade of platelet integrin glycoprotein (GP) IIb/IIIa is a promising new antiplatelet strategy. The binding of ligands or of the ligand-mimetic peptide RGD causes a conformational change of GP IIb/IIIa from the nonactivated to the activated state. Because several blocking agents/inhibitors are ligand-mimetics, ...


    pub_type: 杂志文章


    authors: Peter K,Schwarz M,Ylänne J,Kohler B,Moser M,Nordt T,Salbach P,Kübler W,Bode C

    更新日期:1998-11-01 00:00:00

  • Circulating forms of intercellular adhesion molecule (ICAM)-1 in mice lacking membranous ICAM-1.

    abstract::Mice deficient in intercellular adhesion molecule-1 (ICAM-1), lacking membranous ICAM-1, show a normal development but abnormalities of inflammatory and immune functions. Although the membrane-bound form of ICAM-1 is not detectable in the mutant strain, circulating ICAM-1 (cICAM) is present in serum from ICAM-1-defici...


    pub_type: 杂志文章


    authors: van Den Engel NK,Heidenthal E,Vinke A,Kolb H,Martin S

    更新日期:2000-02-15 00:00:00

  • Phase 2 study of subcutaneous omacetaxine mepesuccinate after TKI failure in patients with chronic-phase CML with T315I mutation.

    abstract::Chronic myeloid leukemia (CML) patients with the BCR-ABL T315I mutation do not benefit from therapy with currently approved tyrosine kinase inhibitors. Omacetaxine mepesuccinate is a protein synthesis inhibitor that has demonstrated activity in cells harboring the T315I mutation. This phase 2 trial assessed the effica...


    pub_type: 杂志文章,多中心研究


    authors: Cortes J,Lipton JH,Rea D,Digumarti R,Chuah C,Nanda N,Benichou AC,Craig AR,Michallet M,Nicolini FE,Kantarjian H,Omacetaxine 202 Study Group.

    更新日期:2012-09-27 00:00:00

  • Overexpression and involvement in migration by the metastasis-associated phosphatase PRL-3 in human myeloma cells.

    abstract::Multiple myeloma (MM) is characterized by accumulation and dissemination of malignant plasma cells (PCs) in the bone marrow (BM). Gene expression profiling of 2 MM cell lines (OH-2 and IH-1) indicated that expression of PRL-3, a metastasis-associated tyrosine phosphatase, was induced by several mitogenic cytokines. Cy...


    pub_type: 杂志文章,多中心研究


    authors: Fagerli UM,Holt RU,Holien T,Vaatsveen TK,Zhan F,Egeberg KW,Barlogie B,Waage A,Aarset H,Dai HY,Shaughnessy JD Jr,Sundan A,Børset M

    更新日期:2008-01-15 00:00:00

  • Expression and functional role of the proto-oncogene c-kit in acute myeloblastic leukemia cells.

    abstract::The c-kit proto-oncogene encodes a receptor tyrosine kinase that is thought to play an important role in hematopoiesis. In a series of human acute myeloblastic leukemia (AML), the expression of the c-kit proto-oncogene and its product was studied by means of Northern blot and immunoblot analyses. The c-kit mRNA was ex...


    pub_type: 杂志文章


    authors: Ikeda H,Kanakura Y,Tamaki T,Kuriu A,Kitayama H,Ishikawa J,Kanayama Y,Yonezawa T,Tarui S,Griffin JD

    更新日期:1991-12-01 00:00:00

  • CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.

    abstract::In the maternal decidua, natural killer (NK) cells, characterized by lack of CD16, are found in direct contact with the fetal extravillous trophoblasts (EVTs). It is yet unknown which factors contribute to the specific homing of this unique NK subset to the decidua. In this study we analyze the chemokine receptor repe...


    pub_type: 杂志文章


    authors: Hanna J,Wald O,Goldman-Wohl D,Prus D,Markel G,Gazit R,Katz G,Haimov-Kochman R,Fujii N,Yagel S,Peled A,Mandelboim O

    更新日期:2003-09-01 00:00:00

  • High frequency of monoallelic retinoblastoma gene deletion in B-cell chronic lymphoid leukemia shown by interphase cytogenetics.

    abstract::Inactivation of the retinoblastoma tumor-suppressor gene (RB-1) has been associated with tumorigenicity in various human malignancies. In chronic lymphoid leukemias of B-cell origin (B-CLL) an involvement of RB-1 has been suggested based on cytogenetic data. We examined RB-1 and its chromosomal locus 13q14 in 35 cases...


    pub_type: 杂志文章


    authors: Stilgenbauer S,Döhner H,Bulgay-Mörschel M,Weitz S,Bentz M,Lichter P

    更新日期:1993-04-15 00:00:00

  • Endothelial Smad4 restrains the transition to hematopoietic progenitors via suppression of ERK activation.

    abstract::In mouse mid-gestational embryos, definitive hematopoietic stem progenitor cells are derived directly from a very small proportion of the arterial endothelium. However, the physiological mechanisms restraining excessive endothelial-hematopoietic transition remain elusive. We show here that genetic deletion of Smad4 fr...


    pub_type: 杂志文章


    authors: Lan Y,He W,Li Z,Wang Y,Wang J,Gao J,Wang W,Cheng T,Liu B,Yang X

    更新日期:2014-04-03 00:00:00

  • Prognostic implications of evaluation for lymph node involvement by T-cell antigen receptor gene rearrangement in mycosis fungoides.

    abstract::We investigated the correlation between the detection of clonal rearrangement of the T-cell antigen receptor gene (TCRR) in lymph node tissue with histopathologic lymph node classification in 33 patients with mycosis fungoides with and without the Sezary Syndrome. We analyzed DNA extracted from lymph nodes that were h...


    pub_type: 杂志文章


    authors: Lynch JW Jr,Linoilla I,Sausville EA,Steinberg SM,Ghosh BC,Nguyen DT,Schechter GP,Fischmann AB,Ihde DC,Stocker JL

    更新日期:1992-06-15 00:00:00

  • Treatment of non-obese diabetic (NOD)/Severe-combined immunodeficient mice (SCID) with flt3 ligand and interleukin-7 impairs the B-lineage commitment of repopulating cells after transplantation of human hematopoietic cells.

    abstract::Until recently, the identification of cellular factors that govern the developmental program of human stem cells has been difficult due to the absence of repopulation assays that detect human stem cells. The transplantation of human bone marrow (BM) or cord blood (CB) into non-obese diabetic (NOD)/severe-combined immu...


    pub_type: 杂志文章


    authors: Kapp U,Bhatia M,Bonnet D,Murdoch B,Dick JE

    更新日期:1998-09-15 00:00:00

  • Interaction between FXIII and fibrinogen.

    abstract::In this issue of Blood, Smith and colleagues report on the functional role of the interaction between these 2 proteins by studying the involved binding sites responsible for clot stabilization.(1) ...


    pub_type: 评论,杂志文章


    authors: Kohler HP

    更新日期:2013-03-14 00:00:00

  • CD4+CD25+ regulatory T-cell deficiency in patients with hepatitis C-mixed cryoglobulinemia vasculitis.

    abstract::Patients who are chronically infected with hepatitis C virus (HCV) often develop mixed cryoglobulinemia (MC), a B-cell proliferative disorder with polyclonal activation and autoantibody production. We investigated if MC is associated with a deficit of CD4(+)CD25(+) immunoregulatory T (Treg) cells, which have been show...


    pub_type: 杂志文章


    authors: Boyer O,Saadoun D,Abriol J,Dodille M,Piette JC,Cacoub P,Klatzmann D

    更新日期:2004-05-01 00:00:00

  • Antitumor effect of donor marrow graft rejection induced by recipient leukocyte infusions in mixed chimeras prepared with nonmyeloablative conditioning: critical role for recipient-derived IFN-gamma.

    abstract::Some patients lose chimerism following nonmyeloablative hematopoietic cell transplantation (HCT), yet, surprisingly, enjoy sustained tumor remissions. We hypothesized that host-versus-graft (HVG) alloresponses might induce antitumor effects against recipient tumors. We explored this question in mice by administering r...


    pub_type: 杂志文章


    authors: Rubio MT,Kim YM,Sachs T,Mapara M,Zhao G,Sykes M

    更新日期:2003-09-15 00:00:00