Thymidine and methionine syntheses in pregnant rats exposed to nitrous oxide.

Abstract:

:The dose-dependent effects of nitrous oxide on thymidine and methionine syntheses were investigated in pregnant rats. Female Sprague-Dawley rats were exposed on day 9 of gestation to 0.75%, 7.5%, or 75% nitrous oxide for 24 h. Immediately and 72 h after exposure, a deoxyuridine-suppression test was performed on maternal bone marrow and a methionine synthetase assay was performed on maternal liver to assess thymidine and methionine syntheses, respectively. Inhibition of thymidine synthesis was seen after exposure to 7.5% and 75%, but not after 0.75%, nitrous oxide. Recovery was complete 72 h after exposure. Methionine synthetase activity was abolished at all concentrations of nitrous oxide tested and did not return to control values 72 h after exposure. Fetal weight and gross appearance were not affected by exposure to nitrous oxide; however, the observed decrease in thymidine and methionine syntheses after nitrous oxide exposure may account for its teratogenic effects.

journal_name

Anesth Analg

journal_title

Anesthesia and analgesia

authors

Baden JM,Rice SA,Serra M,Kelley M,Mazze R

subject

Has Abstract

pub_date

1983-08-01 00:00:00

pages

738-41

issue

8

eissn

0003-2999

issn

1526-7598

journal_volume

62

pub_type

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