Abstract：
:Morris 7777 hepatoma cells, maintained in culture for 5 days in Lewis medium supplemented with 0.1 to 0.35 mM cis-unsaturated fatty acid of the oleic (number of carbon atoms: number of double bonds, 18:1), linoleic (18:2), or arachidonic (20:4) type, were compared to similarly maintained, unsupplemented cells as to ultrastructure and fatty acid composition. The cells of unsupplemented cultures were distinguished by their spherical shape, multilobated nuclei, large nucleoli, and distribution of chromatin. Cellular extensions in the form of pseudopodia and filopodia along with junctional complexes and structures resembling bile canaliculi were evident. The cytoplasmic matrix and cellular organelles appeared normal in morphology. The ultrastructure of fatty acid-supplemented cells differed from unsupplemented hepatoma cells in cell size, location of the nucleus, abundance of endoplasmic reticulum, accumulation of lipid droplets, mitochondrial abnormalities, redistribution of cytoplasmic fibrils, and plasmalemmal extensions. Supplemented cells contained lipid droplets varying in number from a optimum of six to a maximum of greater than 70. The size of these lipid droplets varied from 0.57 +/- 0.34 (S.D.) micrometers for oleic, 1.22 +/- 0.67 micrometers for linoleic, and 0.91 +/- 0.46 micrometers for arachidonic acid-supplemented cells. Variation in the ultrastructure of supplemented cells was also evident. Cytoplasmic vesiculation appeared more frequently and was more prominent in the linoleic acid-supplemented cells. Alterations in the number of surface specializations and nuclear morphology were more pronounced in the arachidonic acid-supplemented cells. Occasional swelling, loss of matrix density, and dilated cristae were evident in mitochondria of oleic acid-supplemented cells. The neutral and phospholipid fractions of arachidonic acid-supplemented cells differed from the unsupplemented cells in the concentrations of 14:0, 15:0, 16:0, 16:1, 17:0, 18:0, 18:1, 18:2, 20:1, 20:3, 20:4, 22:5, and 22:6 fatty acids. The most significant change was detected in the higher level, 26- or 8-fold, of fatty acids 20:3 omega 6 and 22:6 in the phospholipid fraction of arachidonic acid-supplemented cells.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Abbas MK,Yoo TJ,Viles Jsubject
Has Abstractpub_date
1982-11-01 00:00:00pages
4639-49issue
11eissn
0008-5472issn
1538-7445journal_volume
42pub_type
杂志文章相关文献
CANCER RESEARCH文献大全abstract：:Although peptide immunization often leads to the induction of strong T-cell responses, it is seldom effective against established tumors. One possibility is that these T-cell responses are not strong enough or do not last sufficiently long to have an effect in tumor eradication. Here, we examined the role of synthetic...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2003-06-15 00:00:00
abstract：:A quantitative assay for the biochemical transformation of thymidine kinaseless mouse cells (N cIA cI10 cells) to an enzyme-positive phenotype by herpes simplex virus has been used to examine the effect of the carcinogen nitrosomethylurea on cell transformation. Exposure of cells to the chemical carcinogen, followed b...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:Malignant and uninvolved human breast tissues were maintained in organ culture for 3 to 6 days. Under these conditions, the three-dimensional glandular architecture is maintained with the least disruption of tissue integrity. The biosynthesis and release of glycoproteins were studied by using the incorporation of [14C...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1982-11-01 00:00:00
abstract：:The pancreatic carcinogen N-nitrosobis(2-oxopropyl)-amine was administered to Syrian golden hamsters after cholecystoduodenostomy and choledochostomy to investigate a possible bile reflux mechanism as a factor in pancreatic carcinogenesis. The induced lesions were similar in morphology, multiplicity, and distribution ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:The effects of tumor-promoting phorbol diesters on ornithine decarboxyalse (ODC) activity and DNA synthesis in normal and chemically transformed hamster embryo fibroblasts (HEF) in culture were studied. Only those phorbol diesters with promoting activity in mouse skin induced ODC in HEF. ODC was induced in both cell t...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1977-11-01 00:00:00
abstract：:When L1210 cells growing logarithmically were exposed for 8 hr to a nonlethal dose of 5-fluorouracil (FU) (0.25 microgram/ml), the percentage of cells in S phase increased from 74.9% in the asynchronous culture to 93% in the FU-treated culture. This resulted in increased cell-kill by S-phase-specific inhibitors [1-bet...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Acute lymphoblastic leukemia (ALL) is the most common pediatric malignancy and constitutes 15% of adult leukemias. Although overall prognosis for pediatric ALL is favorable, high-risk pediatric patients and most adult patients have significantly worse outcomes. Multiagent chemotherapy is standard of care for both pedi...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-10-2238
更新日期：2011-02-15 00:00:00
abstract：:We describe a method to postlabel, in vivo, biotinylated monoclonal antibodies pretargeted onto tumor deposits when most of the non-tumor-bound antibodies have already been cleared as avidin-bound complexes. The application of this principle to tumor detection by immunoscintigraphy was tested in 20 patients with histo...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:Interleukin 15 (IL-15) is a novel cytokine that shares no homology with IL-2, but it requires the use of beta and gamma chains of the IL-2 receptor complex for binding and signaling. In vitro studies have shown induction of CTL and lymphokine-activated killer (LAK) cell activity in peripheral blood mononuclear cells (...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The acute hemorrhagic necrosis of tumor nodules caused by the systemic administration of recombinant human tumor necrosis factor alpha (rhTNF-alpha) has been partially attributed to changes in tumor neovascularity. In this study, the effects of rhTNF-alpha were tested on primary autochthonous sarcomas induced in C57BL...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-04-15 00:00:00
abstract：:A balanced lipid metabolism is crucial for all cells. Disturbance of this homeostasis by nonphysiological intracellular accumulation of fatty acids can result in apoptosis. This was proven in animal studies and was correlated to some human diseases, like lipotoxic cardiomyopathy. Some metabolic mechanisms of lipo-apop...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-03-3149
更新日期：2004-06-01 00:00:00
abstract：:The expression of monomorphic determinants of the histocompatibility leukocyte antigens (HLA) class I antigens by human malignant tumor cells was studied in tissue specimens of 70 primary tumor lesions obtained from patients with carcinoma of the breast (41 patients), colon (8 patients), urinary bladder (8 patients), ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:TGF-β has limited effects on ovarian cancer cells, but its contributions to ovarian tumor growth might be mediated through elements of the tumor microenvironment. In the present study, we tested the hypothesis that TGF modulates ovarian cancer progression by modulating the contribution of cancer-associated fibroblasts...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-13-0023
更新日期：2013-08-15 00:00:00
abstract：:Heregulin (HRG) is a member of the neuregulin family of ligands that have been shown to interact with and activate erbB receptors. A transgenic mouse model in which full-length HRG is overexpressed has proven that this protein can induce carcinomas in the murine mammary gland. These tumors display a high level of apop...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2000-07-15 00:00:00
abstract：:Astrocytoma, the most common brain tumor in humans, is usually malignant and virtually incurable. Two types of malignant astrocytomas can be distinguished histopathologically: anaplastic astrocytoma and glioblastoma multiforme. Studies using DNA markers that detect restriction fragment length polymorphisms have shown ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1990-09-15 00:00:00
abstract：:Combining microtubule-targeting antimitotic drugs with targeted apoptosis potentiators is a promising new chemotherapeutic strategy to treat cancer. In this study, we investigate the cellular mechanism by which navitoclax (previously called ABT-263), a Bcl-2 family inhibitor, potentiates apoptosis triggered by paclita...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-10-4336
更新日期：2011-07-01 00:00:00
abstract：:Centrosome amplification is a hallmark of virtually all types of cancers, including solid tumors and hematologic malignancies. Cancer cells with extra centrosomes use centrosome clustering (CC) to allow for successful division. Because normal cells do not rely on this mechanism, CC is regarded as a promising target to...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-16-1144
更新日期：2016-11-15 00:00:00
abstract：:The transcription factor Dmp1 is a Ras/HER2-activated haplo-insufficient tumor suppressor that activates the Arf/p53 pathway of cell-cycle arrest. Recent evidence suggests that Dmp1 may activate p53 independently of Arf in certain cell types. Here, we report findings supporting this concept with the definition of an A...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-11-2410
更新日期：2012-04-01 00:00:00
abstract：:Matrix metalloproteinase-2 (MMP-2) expression is often up-regulated in advanced cancers and known to play an important role in tumor angiogenesis. We previously showed that adenoviral-mediated delivery of siRNA for MMP-2 (Ad-MMP-2-Si) inhibited lung cancer growth, angiogenesis, and metastasis. In this study, we invest...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-07-6612
更新日期：2008-06-15 00:00:00
abstract：:The expression of osteopontin (OPN), CD44 variants, and integrins has been correlated with tumorigenesis and metastasis. Here we show that these proteins cooperate to enhance cell motility. First, we demonstrate that several different CD44 variants bind to OPN in an arginine-glycineaspartic acid-independent manner, bu...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Multiple endocrine neoplasia 2B (MEN 2B) is an inherited syndrome of early onset endocrine tumors and developmental anomalies. The disease is caused primarily by a methionine to threonine substitution of residue 918 in the kinase domain of the RET receptor (2B-RET); however, the molecular mechanisms that lead to the d...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-06-3329
更新日期：2006-11-15 00:00:00
abstract：:Neuropilin-1 (NP1), in conjunction with plexins, promotes axon repulsion by binding to semaphorin 3A (SEMA3A). Although NP1 is expressed in carcinoma cells, its functions have remained elusive, and neither SEMA3A nor plexin expression has been explored in cancer. Here we provide evidence that breast carcinoma cells su...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:The presence of a non-small cell lung carcinoma (NSCLC)-related gene or genes on chromosome band 11p15.5 is of particular interest, given the specific loss of heterozygosity (LOH) measured in this region for lung as well as many other pediatric and adult neoplasms. We have undertaken high-density polymorphic marker an...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Low income, poor diet, obesity, and a lack of exercise are interrelated lifestyle factors that can profoundly alter our biologic make up to increase cancer risk, growth, and development. We recently reported a potential mechanistic link between carbohydrate-derived metabolites and cancer, which may provide a biologic ...
journal_title：Cancer research
pub_type： 杂志文章,评审
doi：10.1158/0008-5472.CAN-15-0169
更新日期：2015-05-15 00:00:00
abstract：:Many epithelial tumors show deletion of the short arm of chromosome 8 that is related to aggressive disease or adverse prognosis. In undissected samples of urothelial cell carcinoma of the bladder, at least two regions of loss of heterozygosity (LOH) were identified previously within a small region of 8p11-p12. LOH an...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:FET cells, derived from an early-stage colon carcinoma, are nontumorigenic in athymic mice. Stable transfection of a dominant-negative transforming growth factor beta (TGFbeta) type II receptor (DNRII) into FET cells that express autocrine TGFbeta shows loss of TGFbeta signaling and increased tumorigenicity in vivo in...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-07-5348
更新日期：2008-05-01 00:00:00
abstract：:There is no effective treatment for prostate cancer arising after androgen ablation. Previous studies have analyzed the short-term effects of androgen ablation on the immune system and suggest an abatement of immune suppression by hormone removal. Because castration-resistant disease can arise years after treatment, i...
journal_title：Cancer research
pub_type： 杂志文章
doi：10.1158/0008-5472.CAN-09-2490
更新日期：2010-05-01 00:00:00
abstract：:Radioimmunoassay has been used to measure acetylated and deacetylated deoxyguanosine C-8 DNA adducts of 2-acetylaminofluorene in liver and kidney DNA of male Wistar-Furth rats fed a dietary regimen of 0.02 or 0.04% 2-acetylaminofluorene. When adduct formation was monitored during continuous feeding up to 60 days, subs...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:The monoclonal antibodies (MAbs) 323/A3 and 17-1A both recognize a 40-kDa carcinoma-associated epithelial glycoprotein (EGP40). MAb 17-1A has been used in many therapeutic trials as an immunotherapeutic agent to combat advanced colorectal cancer, and about 5-10% overall responses have been observed. It has been shown ...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Human intraepithelial lymphocytes (IEL), predominantly CD8+ T-lymphocytes located between intestinal epithelial cells (EC), may represent the first-line immune defense against colon cancer. The mechanism by which IEL bind to the colon cancer line, DLD-1, was evaluated. A larger fraction of IEL than peripheral blood mo...
journal_title：Cancer research
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
