Abstract:
:Recently, bovine pulmonary microvascular endothelial cells (PMV) were shown to contain cytokeratin 8 and 19 intermediate filaments (Patton et al., 1990). In this study, we examine the effect of culture contiguity and vasoactive agents on the content and assembly of cytokeratins in PMV. Immunofluorescent staining of PMV cultures show a progressive increase in cytokeratin filament assembly. In freshly plated PMV, keratin appears as hazy staining (less than 4 hr) and later organizes into keratin 'plaques' (4 days) associated with cell-cell contacts; post confluent (greater than 7 days) PMV cultures contain fully assembled cytokeratin filaments which extend to the cell periphery and approach filaments in apposed cells. Vimentin filaments are also present in freshly plated PMV cultures but unlike cytokeratins, become less filamentous at confluency. This cell density-dependent modulation of cytokeratins is also demonstrated by densitometric analysis of autoradiographs of 35S-methionine labeled keratins in which PMV keratin content is elevated at high cell densities, while vimentin content remains constant. Desmoplakins I and II, components of desmosomes, could not be demonstrated in PMV by immunoblotting. PMV treated with permeability modulating agents (4 x 10(-3) M EGTA, 1 microM cytochalasin B, 1 microM bradykinin, 1 microM A23187, and 1 microM PMA) exhibit border retraction and altered keratin filament staining. From these studies we conclude: 1) cytokeratin 8 and 19 containing intermediate filaments are present in confluent PMV cultures with vimentin but without desmosomes, 2) the state of assembly of PMV cytokeratin and vimentin filaments appears to be oppositely affected by culture contiguity, and 3) treatment of monolayers with vasoactive agents alters the state of assembly of cytokeratin filaments. We speculate that modulation of cytokeratin assembly in PMV may be involved in regulation of pulmonary microvascular structure and function.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Alexander JS,Patton WF,Yoon MU,Shepro Ddoi
10.1016/0040-8166(91)90069-6subject
Has Abstractpub_date
1991-01-01 00:00:00pages
141-50issue
2eissn
0040-8166issn
1532-3072pii
0040-8166(91)90069-6journal_volume
23pub_type
杂志文章相关文献
TISSUE & CELL文献大全abstract::A panel of 40 monoclonal antibodies was constructed in response to cationic endothelial cell growth factor (c-ECGF), the cationic peptide mitogen isolated from endothelial mitogen. The monoclonal antibodies were assayed by dot blot for immunoreactivity to various other peptide angiogenic factors. The panel of monoclon...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(87)90040-1
更新日期:1987-01-01 00:00:00
abstract::Nuclei in the giant polyploid silk gland cells of Calpodes ethlius grow by endomitosis and can develop hundreds of branches during larval life. The shape of the these nuclei is characteristic for each region of the gland. We have found shape to be correlated with arrangement of the nuclear matrix. Scanning electron mi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(91)90036-s
更新日期:1991-01-01 00:00:00
abstract::Spermatogenesis and the sperm structure of the terebrantian Aeolothrips intermedius Bagnall are described. Spermatogenesis consists of two mitotic divisions; the second is characterized by the loss of half of the spermatids, which have pyknotic nuclei. Early spermatids have two centrioles, but when spermiogenesis star...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2010.04.008
更新日期:2010-08-01 00:00:00
abstract::The spermatozoon of the Onychophoran Peripatopsis moseleyi is described. This cell is characterized by the general filiform shape, the absence of an acrosome, the presence of mitochondrial derivatives, of an annulus and of nine accessory tubules and a manchette of microtubules around the axoneme. All of these characte...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(76)90038-0
更新日期:1976-01-01 00:00:00
abstract::The reproductive system of the female Urodacus manicatus (Scorpionidae) is described. Situated on the ventral side of the ovariuterus tubes are buds and diverticulae belonging to three developmental phases: (1) Bud-like, rudimentary diverticulae that will form future generations. (2) Embryonic diverticulae that will h...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90060-4
更新日期:1994-10-01 00:00:00
abstract::Responses of cells in the tick salivary gland to parasitism by Theileria parva were studied by electron microscopy. The gland is composed of three distinct types of acini (I, II, III) which together include ten or more different cell types. Of some 30 infected cells observed in the present study, all were E-cells of a...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(82)90035-0
更新日期:1982-01-01 00:00:00
abstract::Myosin as well as actin filaments could be seen in negatively stained preparations of fresh chicken gizzard homogenized in buffered KCI (I=0.12, pH 6.85), in a 1 : 1 ratio. Myosin filaments were also present when homogenates were diluted (1 : 9) with solutions containing additional Mg(++) and ATP provided naturally oc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(69)80007-8
更新日期:1969-01-01 00:00:00
abstract::Germ cells' proliferation during testicular organogenesis in Wistar rat embryos and neonates [14.5, 18.5, 20.5 days post conception (dpc), birth (day 0), 1, 3, 5, 7 days post partum (dpp)] was evaluated via immunohistochemistry, using the PCNA and Ki-67 nuclear antibodies. Estimation of the reactive/total cell ratio, ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.09.004
更新日期:2008-02-01 00:00:00
abstract::Pyridoxal-5'-phosphate, the active form of vitamin B6, is associated with activities of several enzymes and the treatment of various neurological disorders. Here, we investigated the effects of pyridoxine on the immunoreactivity and protein levels of γ-aminobutyric acid (GABA)-synthesizing and degradation enzymes such...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.09.005
更新日期:2019-12-01 00:00:00
abstract::The hydrozoa life cycle is characterized, in normal conditions, by the alternation of a post-larval benthic polyp and an adult pelagic medusa; however, some species of Hydrozoa react to environmental stress by reverting their life cycle: i.e. an adult medusa goes back to the juvenile stage of polyp. This very uncommon...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(03)00028-4
更新日期:2003-06-01 00:00:00
abstract::A hydrogenosome is a hydrogen-producing organelle, evolutionary related to mitochondria and is found in Parabasalia protozoa, certain chytrid fungi and certain ciliates. It displays similarities to and differences from mitochondria. Hydrogenosomes are spherical or slightly elongated organelles, although very elongated...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/j.tice.2009.01.001
更新日期:2009-06-01 00:00:00
abstract::Gap junctions are plasma membrane specializations characterized as aggregates of intramembranous particles in two apposed membranes meeting particle-to-particle in the 2-4 nm intermembrane 'gap'. Recent thin-section and freeze-fracture evidence has revealed significant structural variations of gap junctional structure...
journal_title:Tissue & cell
pub_type: 杂志文章,评审
doi:10.1016/0040-8166(77)90001-5
更新日期:1977-01-01 00:00:00
abstract::The contribution of radiosensitive cells and macrophages to myocardial immunopathology has been studied in rats inoculated with Trypanosoma cruzi, Y strain. Immunodepression was induced by gamma irradiation and depletion of radioresistant macrophages was achieved by silica, a selective cytotoxic agent for macrophages....
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1054/tice.1999.0040
更新日期:1999-06-01 00:00:00
abstract::Using a monoclonal antibody (GT 335) we previously demonstrated that glutamylation is a predominant posttranslational modification of alpha and beta tubulin isoforms in the axoneme of mouse spermatozoa (Fouquet et al., Cell Motil. Cytoskel. 27, 49, 1994). However, we noted that the staining intensity and/or distributi...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(95)80053-0
更新日期:1995-06-01 00:00:00
abstract::A culture technique for the in vitro growth of antennal cells from honeybee is described. On the basis of morphological and immunocytochemical criteria, the cultured cells could be classified into neural and non-neural cells. Neural cells (type D) exhibited the main morphological features of insect olfactory receptor ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(94)90007-8
更新日期:1994-08-01 00:00:00
abstract::Docetaxel (DCT) is an anticancer drug which acts by disrupting microtubule dynamics in the highly mitotic cancer cells. Thus, this drug has a potential to affect function and organization of tissues exhibiting high cellular turnover. We investigated, in the rabbit, the effects of a single human equivalent dose (6.26 m...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2014.04.005
更新日期:2014-06-01 00:00:00
abstract::The teleost species Notopterus notopterus (Pallas) possess bundled photoreceptors in their retina. It was found that the margin of the inner portion (the vitreal half) of photoreceptor bundles emits thin processes. Each process terminates on the contralateral photoreceptor bundle, or the processes from adjacent photor...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2019.03.003
更新日期:2019-06-01 00:00:00
abstract::The ultrastructural localization of two types of biochemically characterized phenol oxidase activity is described in the larva of the sheep blowfly, Lucilia cuprina. Cuticular tyrosinase activity (enzyme A) is seen in epicuticular filaments and procuticle. Procuticle activity can be detected only after a presumed proc...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90073-0
更新日期:1988-01-01 00:00:00
abstract::We established a human pancreatic carcinoma cell line, designated SPH, from cancerous ascites of a 57-year-old male patient with ductal adenocarcinoma of the pancreas. The cells have been cultured for 32 months with RPMI-1640 medium supplemental with 10% fetal calf serum. The population doubling time of this cell line...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.04.001
更新日期:2007-08-01 00:00:00
abstract::The role of phagocytosis in eliminating apoptotic spermatogenic cells caused by mono(2-ethylhexyl) phthalate (MEHP) was studied. Twenty-one-day-old C57Bl/6N male mice were given a single dose of 800 mg/kg MEHP in corn oil by oral gavage and sacrificed at 1, 3, 5, 7 and 9 days after initial exposure. At the same time, ...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2007.04.002
更新日期:2007-08-01 00:00:00
abstract::In this study we compared the effects of the Toll-like receptor (TLR) ligands lipopolysaccharide (LPS), flagellin, the synthetic bacterial triacylated lipopeptide Pam3-Cys-Ser-Lys4 (Pam3CSK4), Polyinosinic:polycytidylic acid (Poly I:C), and macrophage-activating lipopeptide (MALP-2), which are TLR4, TLR5, TLR1/2, TLR3...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2016.04.002
更新日期:2016-08-01 00:00:00
abstract::We have recently shown that down-regulation of mouse Thy28 (mThy28) protein expression appears to be accompanied by apoptotic processes. Thymocytes from mice contain moderate amounts of mThy28 protein and undergo proliferation, differentiation, or apoptosis during murine thymic maturation. As a first step to examine t...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(03)00073-9
更新日期:2003-12-01 00:00:00
abstract::Granulocytes (GRs) and/or plasmatocytes (PLs), the two major immunocytes in arthropods, participate in cellular encapsulation of foreign tissue. Although gap and desmosome junctions have been reported in insect capsules, smooth septate junctions are being reported for the first time by both thin section and freeze-fra...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(88)90064-x
更新日期:1988-01-01 00:00:00
abstract::The ovoid cells of the branchial heart complex of Sepia officinalis L. were investigated with respect to their role in detoxification processes. The electron microscopical localization of in situ injected ferritin in the endocytotic-lysosomal system, and the fluorescence microscopical localization of protein-bound Eva...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(98)80085-2
更新日期:1998-12-01 00:00:00
abstract::The expression and localization of gap junctional proteins connexin (Cx) 26, 32, and 43 was examined in human dental pulp. Dental pulp tissues were obtained from human third molars immediately after extraction. Some pulp tissues were used for cell culture, and the rest for histological observations. Immunostaining for...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s0040-8166(02)00028-9
更新日期:2002-06-01 00:00:00
abstract::Pelagic amphipods belonging to the genus Phronima have four compound eyes; two lateral eyes and two large transparent medial eyes which comprise the entire top of the head. The eyes are structurally similar but the crystalline cones of the medial eyes are more than twenty times as long as those of the lateral eyes, re...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(77)90010-6
更新日期:1977-01-01 00:00:00
abstract::The fine structure of the principal and ancillary metathoracic flight muscle fibres in the adult male of a strepsipteran, Elenchus tenuicornis, is described. Power-producing dorsal longitudinal and dorso-ventral flight muscles show features consistent with myoneural asynchrony: myofibrils are large and discrete and ar...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(84)90072-7
更新日期:1984-01-01 00:00:00
abstract::Female adults of the bagworm moth, Eumeta variegata, lack wings completely, whereas male adults of this species have functional wings. We previously found that ecdysteroid induces apoptotic events in the female wing rudiment of E. variegata in vitro, whereas the male wing discs cultured with 20-hydroxyecdysone (20E) u...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/j.tice.2011.01.003
更新日期:2011-06-01 00:00:00
abstract::Using immunoelectronmicroscopy we analyzed qualitative and quantitatively the intracellular distribution of bothropasin, hemorrhagic factor 2 (HF2) and hemorrhagic factor 3 (HF3) in the venom secretory cells from adult snakes in the active (7 days after venom extraction) and in the resting (without venom extraction fo...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/s004081660200068x
更新日期:2002-12-01 00:00:00
abstract::A novel type of pore canal is described from the cuticle of three species of Gammarus. Each canal passes from the epidermis vertically through the endocuticle and exocuticle, and in the most distal layers of the latter is slightly expanded. Before entering the epicuticle the canal narrows, forming a neck the base of w...
journal_title:Tissue & cell
pub_type: 杂志文章
doi:10.1016/0040-8166(78)90053-8
更新日期:1978-01-01 00:00:00