Effects of Toll-like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis.

Abstract:

:In this study we compared the effects of the Toll-like receptor (TLR) ligands lipopolysaccharide (LPS), flagellin, the synthetic bacterial triacylated lipopeptide Pam3-Cys-Ser-Lys4 (Pam3CSK4), Polyinosinic:polycytidylic acid (Poly I:C), and macrophage-activating lipopeptide (MALP-2), which are TLR4, TLR5, TLR1/2, TLR3, and TLR2/6 agonists, respectively, on cell morphology and phagocytosis of zymosan particles, derived from Saccharomyces cerevisiae, and rich in fungal PAMPs including beta-glucan, mannose, and chitin. LPS, Pam3CSK4, and MALP-2 induced an activated macrophage phenotype and enhanced zymosan phagocytosis. In contrast, flagellin and Poly I:C, respectively, had little effect on cell morphology and phagocytosis. We examined the role of scavenger receptor A (SR-A) on zymosan phagocytosis. Cells cultured in medium alone expressed SR-A, and LPS induced further expression of the receptor. We also observed inhibitory effects of scavenger receptor antagonists fucoidan, dextran sulphate, and Polyinosinic (Poly I), respectively, on zymosan phagocytosis of cells in medium alone and those pre-treated with LPS. We conclude that exposure to specific TLR ligands impacts both cellular morphology and phagocytic capacity, and that scavenger receptors contribute to zymosan ingestion as well as LPS-induced augmentation of phagocytosis.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Sigola LB,Fuentes AL,Millis LM,Vapenik J,Murira A

doi

10.1016/j.tice.2016.04.002

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

389-96

issue

4

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(16)30084-2

journal_volume

48

pub_type

杂志文章
  • Analysis of the morphology and distribution of argentaffin, argyrophil and insulin-immunoreactive endocrine cells in the small intestine of the adult opossum Didelphis aurita (Wied-Neuwied, 1826).

    abstract::The aim of this study was to identify and quantify the argyrophil, argentaffin and insulin-immunoreactive cells (IIC) in the small intestine of the opossum Didelphis aurita. Seven adult male specimens of opossums were investigated. The animals were captured, and their blood insulin levels were determined. After euthan...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2012.04.008

    authors: Basile DR,Novaes RD,Marques DC,Fialho MC,Neves CA,Fonseca CC

    更新日期:2012-10-01 00:00:00

  • Fusion of myocytes with larval muscle remnants during flight muscle development in the Colorado beetle.

    abstract::The transformation of the slow contracting larval m. obliquus lateralis caudalis II during metamorphosis into the asynchronous indirect flight muscle, m. obliquus lateralis dorsalis, in the Colorado beetle, Leptinotarsa decemlineata, was examined by electron microscopy. Particular attention was paid to the fate of the...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(86)90066-2

    authors: Smit WA,Velzing EH

    更新日期:1986-01-01 00:00:00

  • In vitro effects of juvenile hormone analog on wing disc morphogenesis under ecdysteroid treatment in the female-wingless bagworm moth Eumeta variegata (Insecta: Lepidoptera, Psychidae).

    abstract::Female adults of the bagworm moth, Eumeta variegata, lack wings completely, whereas male adults of this species have functional wings. We previously found that ecdysteroid induces apoptotic events in the female wing rudiment of E. variegata in vitro, whereas the male wing discs cultured with 20-hydroxyecdysone (20E) u...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2011.01.003

    authors: Niitsu S,Lobbia S,Kamito T

    更新日期:2011-06-01 00:00:00

  • Recycling of mast cells following degranulation in vitro: an ultrastructural study.

    abstract::Mature mast cells, isolated from the rat peritoneal cavity, were placed into suspension culture, either as resting or after degranulation by exposure to compound 48/80, and were maintained for up to 63 hr. No mitotic cells were observed, and cell number was conserved. The culture conditions did not cause spontaneous d...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(82)90012-x

    authors: Burwen SJ

    更新日期:1982-01-01 00:00:00

  • Histochemical localization of biogenic monoamines in the posterior salivary glands of octopods.

    abstract::Tubules of the octopod posterior salivary gland are lined by two distinct epithelia, type A and B of which type A is predominant. A positive chromaffin reaction is given only by a small proportion of columnar cells of the type A epithelium, and is apparently associated with the large (3mu) cytoplasmic granules of thes...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(71)80040-x

    authors: Matus AI

    更新日期:1971-01-01 00:00:00

  • Partial development of the steroidogenic ultrastructural features in degenerative corpora lutea after a single injection of pituitary extract in the Western painted turtle (Chrysemys picta).

    abstract::Pituitary glands were removed from sexually mature female turtles (Chrysemys picta) and they were injected intraperitoneally (i.p.) into other mature females of the same species (experimental). In addition mature females of the same species received saline injection only (controls). Initially all the turtles used in t...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2006.02.001

    authors: Mahmoud IY,Ba-Omar T,Alkindi A

    更新日期:2006-06-01 00:00:00

  • Localization of calcium in an annelid visceral muscle by pyroantimonate deposition and by x-ray microprobe analysis.

    abstract::The loci of calcium distribution in Nereis pharyngeal visceral muscle have been examined by cytochemical precipitation using potassium pyroantimonate. In Na-, Ca- and Mg-free media, pyroantimonate incubation was used to pinpoint loci of intracellularly bound calcium. This method also revealed heavy deposition on the i...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(81)90024-0

    authors: Alohan FI,Huddart H

    更新日期:1981-01-01 00:00:00

  • Germ cell proliferation and apoptosis during testicular regression in a seasonal breeding fish kept in captivity.

    abstract::Cell proliferation and apoptosis regulate germ cells stock and sperm production, eliminate anomalous gametes, and are essential parameters to consider in fish farming. Herein, spermatogenic activity as well as germ cell proliferation and apoptosis were assessed in Leporinus taeniatus, a seasonal breeding species from ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2017.09.003

    authors: Ribeiro YM,Matos SA,Domingos FFT,Santos HBD,Cruz Vieira AB,Bazzoli N,Rizzo E

    更新日期:2017-12-01 00:00:00

  • Acute effects of rat growth hormone (GH), human GH and prolactin on proliferating rat liver cells in vitro: a study of mitotic behaviour and ultrastructural alterations.

    abstract::Examination of livers from transgenic mice over expressing human growth hormone (hGH) revealed numerous alterations including a striking incidence of mitotic figures. The reason for increased proliferation is unclear, but could be related to effects of hGH, which is also acting as a lactogen in rodents. In order to id...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(94)90029-9

    authors: Vergani G,Mayerhofer A,Bartke A

    更新日期:1994-06-01 00:00:00

  • Influence of several extracellular matrix components in primary cultures of bovine mammary epithelial cells.

    abstract::Mammary epithelial cells, obtained from lactating cows, were cultured onto inserts coated with several components of extracellular matrix. The influence of these components upon the maintenance of differentiation has been determinated. Every day, alpha S1-casein secretion was measured by radioimmunoassay (RIA) in apic...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(97)80076-6

    authors: Delabarre S,Claudon C,Laurent F

    更新日期:1997-02-01 00:00:00

  • The very rapid induction of filopodia in insect cells.

    abstract::Many insect cells, including epidermis, fat body, ocnocytcs and pericardial cells, can very easily be induced to form long fine processes or filopodia. Filopodia contain microfilaments hut differ from epidermal feet in lacking microtubules and in having a much smaller and uniform diameter. Although they may be 10-30 m...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(87)90014-0

    authors: Locke M

    更新日期:1987-01-01 00:00:00

  • Kupffer's vesicle in Fundulus heteroclitus: a scanning and transmission electron microscope study.

    abstract::Kupffer's vesicle, a transitory organ peculiar to teleost embryos, was investigated in embryos of Fundulus heteroclitus by scanning electron microscopy. The roof of the vesicle is constructed to rather large columnar cells which are in continuity wiht hypoblast cells. Each of the roof cells is adorned with a single lo...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(78)90003-4

    authors: Brummett AR,Dumont JN

    更新日期:1978-01-01 00:00:00

  • Automated oral squamous cell carcinoma identification using shape, texture and color features of whole image strips.

    abstract::Despite profound knowledge of the incidence of oral cancers and a large body of research beyond it, it continues to beat diagnosis and treatment management. Post physical observation by clinicians, a biopsy is a gold standard for accurate detection of any abnormalities. Towards the application of artificial intelligen...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2019.101322

    authors: Rahman TY,Mahanta LB,Das AK,Sarma JD

    更新日期:2020-04-01 00:00:00

  • In situ analysis of microvascular pericytes in hypertensive rat brains.

    abstract::We used immunofluorescence microscopy and isoactin-specific antibodies to characterize the pattern and prevalence of pericytes within the brain microcirculation. Blood pressures of normotensive, Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats were measured prior to sacrifice and pressure-perfusion fixatio...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(88)90002-x

    authors: Herman IM,Jacobson S

    更新日期:1988-01-01 00:00:00

  • Azoramide ameliorates fructose-induced nonalcoholic fatty liver disease in mice.

    abstract::Nonalcoholic fatty liver disease (NAFLD) is a frequent health problem. The insulin resistance and endoplasmic reticulum (ER) stress have been suggested to play important roles in the development and progression of NAFLD. However these processes and correlations have not fully been understood yet. Azoramide, an antidia...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2019.07.001

    authors: Bagci R,Sahinturk V,Sahin E

    更新日期:2019-08-01 00:00:00

  • Endocrine cells and nerves in the stomach of the lizard Podarcis hispanica detected by immunocytochemistry.

    abstract::The general identification of endocrine cells in the stomach of the lizard Podarcis hispanica was carried out by their response to the Grimelius and Masson-Fontana techniques. 11 immunoreactive cell-types, positive for chromogranin-, serotonin-, caerulein/gastrin/ cholecystokinin (CAER/G/CCK)-, glucagon-like-peptide-1...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(92)90041-5

    authors: De Rada OD,Burrell MA,García C,Barrenechea MA,Sesma P

    更新日期:1992-01-01 00:00:00

  • Atypical 'fibrillar' flight muscle in strepsiptera.

    abstract::The fine structure of the principal and ancillary metathoracic flight muscle fibres in the adult male of a strepsipteran, Elenchus tenuicornis, is described. Power-producing dorsal longitudinal and dorso-ventral flight muscles show features consistent with myoneural asynchrony: myofibrils are large and discrete and ar...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(84)90072-7

    authors: Smith DS,Kathirithamby J

    更新日期:1984-01-01 00:00:00

  • Satureja khozestanica essential oil ameliorates progression of diabetic nephropathy in uninephrectomized diabetic rats.

    abstract::Diabetic nephropathy is the common cause of leading to end stage of renal disease (ESRD). Satureja khozestanica essential oil (SKEO) was used as an antioxidant and antidiabetic for the inhibition of diabetic nephropathy. Forty male rats were uninephrectomized and divided in four groups randomly; group one as control, ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2010.11.004

    authors: Tavafi M,Ahmadvand H,Tamjidipoor A,Delfan B,Khalatbari AR

    更新日期:2011-02-01 00:00:00

  • Morphology of the liver of the brook lamprey, Lampetra lamottenii before and during infection with the nematode, Truttaedacnitis stelmioides, hepatocytes, sinusoids, and perisinusoidal cells.

    abstract::Routine light microscopy and transmission and scanning electron microscopy were used to describe and compare the livers of larval lampreys, Lampetra lamottenii before and during infection of the bile ducts by the nematode, Truttaedacnitis stelmioides. The hepatocytes of uninfected animals differ from other lamprey spe...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(92)90073-g

    authors: Eng F,Youson JH

    更新日期:1992-01-01 00:00:00

  • A feasible method for the isolation of mesenchymal stem cells from menstrual blood and their exosomes.

    abstract:BACKGROUND:Mesenchymal stem cells (MSCs) are currently the most promising candidates in regenerative medicine. Nonetheless, there are several limitations associated with the MSC tissue source such as infrequent and invasive bone marrow sampling methods. To overcome these limitations, we have procured MSCs from the mens...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2018.09.010

    authors: Dalirfardouei R,Jamialahmadi K,Mahdipour E

    更新日期:2018-12-01 00:00:00

  • Postembryonic development of leucokinin-like immunoreactive neurons in the moth Spodoptera litura.

    abstract::Antiserum to leucokinin I, a neuropeptide originally isolated from the cockroach Leucophaea maderae, was used for immunocytochemical labeling of neurons in the brain and ventral ganglia of the moth Spodoptera litura during postembryonic development. In the ventral ganglia, leucokinin-like immunoreactivity begins to oc...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(98)80008-6

    authors: Lee BH,Kang H,Kwon D,Park CI,Kim WK,Kim MY

    更新日期:1998-02-01 00:00:00

  • Effect of low radiation dose on the expression and location of aquaporins in rat submandibular gland.

    abstract::Head and neck cancers are common in several regions of the world and the treatment usually includes radiotherapy. This treatment can generate adverse effects to the salivary flow, with a relationship between the dose and the damage caused. Salivary gland cells are highly permeable to water and therefore, they express ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2018.06.006

    authors: Araujo MVT,Spadella MA,Chies AB,Arruda GV,Santos TM,Cavariani MM,Domeniconi RF

    更新日期:2018-08-01 00:00:00

  • Comparison of pulmonary endothelial cell and fibroblast proliferation using time-lapse cinematographic analysis.

    abstract::Time lapse cinematography was used to study and compare the proliferation and migration activity of pulmonary endothelial cells and fibroblasts, two cell types with very different structural and functional properties. Endothelial cells were found to have a more rapid growth rate than fibroblasts. Contributing to the s...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(81)80002-x

    authors: Absher M,Ryan US

    更新日期:1981-01-01 00:00:00

  • The same cell lineage is involved in scale formation and regeneration in the teleost fish Hemichromis bimaculatus.

    abstract::The elasmoid scales of the cichlid fish, Hemichromis bimaculatus, are localized within dermal pockets, the floors of which are separated from the stratum compactum by uninterrupted cellular sheets, the scale-pocket linings (SPL). TEM study of the fry skin shows that the SPL cells originate from the cell population con...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(89)90058-x

    authors: Sire JY

    更新日期:1989-01-01 00:00:00

  • Inflammatory cell profile using different autologous fibrin protocols.

    abstract::Autologous fibrin has been widely used in surgical procedures for both soft and hard tissue repair. There are different protocols and devices to obtain this matrix, with varying centrifugal time, gravity force, speed, angle of the sample tube and spinning radius. The aim of this study was to compare three methods of o...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2020.101407

    authors: Nogueira LS,Martinez EF,Peruzzo DC,Joly JC,Napimoga MH

    更新日期:2020-12-01 00:00:00

  • The development of branched silk gland nuclei.

    abstract::Nuclei in the giant polyploid silk gland cells of Calpodes ethlius grow by endomitosis and can develop hundreds of branches during larval life. The shape of the these nuclei is characteristic for each region of the gland. We have found shape to be correlated with arrangement of the nuclear matrix. Scanning electron mi...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(91)90036-s

    authors: Henderson SC,Locke M

    更新日期:1991-01-01 00:00:00

  • Unusual light-reflecting pigment cells appear in the Xenopus neural tube culture system in the presence of guanosine.

    abstract::Isolation and culture of Xenopus laevis neural tubes resulted in differentiation of melanophores and iridophores from neural crest cells; the differentiated melanophores and iridophores were then maintained in culture for more than 6 months. Guanosine has been reported to promote reflecting platelet formation in melan...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2018.08.003

    authors: Fukuzawa T,Kikuchi Y

    更新日期:2018-10-01 00:00:00

  • Growth factors and chondrogenic differentiation of mesenchymal stem cells.

    abstract::The main purpose of the article is to review recent knowledge about growth factors and their effect on the chondrogenic differentiation of mesenchymal stem cells under in vitro conditions. Damaged or lost articular cartilage leads to progressive debilitation, which have major impact on the life quality of the affected...

    journal_title:Tissue & cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.tice.2011.11.005

    authors: Danišovič L,Varga I,Polák S

    更新日期:2012-04-01 00:00:00

  • Microtubules and the development of the corkscrew region of a mealybug sperm bundle.

    abstract::Two systems of microtubules appear to be functionally significant in the transformation of a small cell into an elongated, acellular 'corkscrew', which represents the anterior region of a sperm bundle of Pseudococcus obscurus. One system of microtubules lies within the cell; it is oriented parallel to the long axis of...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(71)80029-0

    authors: Ross J

    更新日期:1971-01-01 00:00:00

  • Modeling traumatic injury in organotypic spinal cord slice culture obtained from adult rat.

    abstract::Nowadays there are various models of spinal cord injury (SCI) that recreate mechanisms of human SCI. The ex vivo modeling of injury is a robust approach, confronts with less experimental and ethical challenges. Currently almost all ex vivo models are obtained either from embryonic or postnatal animals, which can hardl...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2019.01.002

    authors: Pandamooz S,Salehi MS,Zibaii MI,Safari A,Nabiuni M,Ahmadiani A,Dargahi L

    更新日期:2019-02-01 00:00:00